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http://dx.doi.org/10.5187/jast.2021.e92

Development and evaluation of probiotic delivery systems using the rennet-induced gelation of milk proteins  

Ha, Ho-Kyung (Department of Animal Science and Technology, Sunchon National University)
Hong, Ji-Young (Department of Animal Bioscience (Institute of Agriculture and Life Science), Gyeongsang National University)
Ayu, Istifiani Lola (Department of Food and Nutrition, Daegu University)
Lee, Mee-Ryung (Department of Food and Nutrition, Daegu University)
Lee, Won-Jae (Department of Animal Bioscience (Institute of Agriculture and Life Science), Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.63, no.5, 2021 , pp. 1182-1193 More about this Journal
Abstract
The aims of this study were to develop a milk protein-based probiotic delivery system using a modified rennet-induced gelation method and to determine how the skim milk powder concentration level and pH, which can affect the rennet-induced intra- and inter-molecular association of milk proteins, affect the physicochemical properties of the probiotic delivery systems, such as the particle size, size distribution, encapsulation efficiency, and viability of probiotics in simulated gastrointestinal tract. To prepare a milk protein-based delivery system, skim milk powder was used as a source of milk proteins with various concentration levels from 3 to 10% (w/w) and rennet was added to skim milk solutions followed by adjustment of pH from 5.4 or 6.2. Lactobacillus rhamnosus GG was used as a probiotic culture. In confocal laser scanning microscopic images, globular particles with a size ranging from 10 ㎛ to 20 ㎛ were observed, indicating that milk protein-based probiotic delivery systems were successfully created. When the skim milk powder concentration was increased from 3 to 10% (w/w), the size of the delivery system was significantly (p < 0.05) increased from 27.5 to 44.4 ㎛, while a significant (p < 0.05) increase in size from 26.3 to 34.5 ㎛ was observed as the pH was increased from 5.4 to 6.4. An increase in skim milk powder concentration level and a decrease in pH led to a significant (p < 0.05) increase in the encapsulation efficiency of probiotics. The viability of probiotics in a simulated stomach condition was increased when probiotics were encapsulated in milk protein-based delivery systems. An increase in the skim milk powder concentration and a decrease in pH resulted in an increase in the viability of probiotics in simulated stomach conditions. It was concluded that the protein content by modulating skim milk powder concentration level and pH were the key manufacturing variables affecting the physicochemical properties of milk protein-based probiotic delivery systems.
Keywords
Milk protein; Probiotics; Delivery system; Rennet;
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1 Lee KY, Heo TR. Survival of Bifidobacterium longum immobilized in calcium alginate beads in simulated gastric juices and bile salt solution. Appl Environ Microbiol. 2000;66:869-73. https://doi.org/10.1128/AEM.66.2.869-873.2000   DOI
2 Imafidon GI, Farkye NY. Influence of pH on chymosin action in solutions of different κ-casein variants. J Agric Food Chem. 1994;42:1598-601. https://doi.org/10.1021/jf00044a002   DOI
3 Najera AI, Renobales M, Barron LJR. Effects of pH, temperature, CaCl2 and enzyme concentrations on the rennet-clotting properties of milk: a multifactorial study. Food Chem. 2003;80:345-52. https://doi.org/10.1016/S0308-8146(02)00270-4   DOI
4 Ranadheera CS, Vidanarachchi JK, Rocha RS, Cruz AG, Ajlouni S. Probiotic delivery through fermentation: dairy vs. non-dairy beverages. Fermentation. 2017;3:67. https://doi.org/10.3390/fermentation3040067   DOI
5 Heidebach T, Forst P, Kulozik U. Microencapsulation of probiotic cells by means of rennet-gelation of milk proteins. Food Hydrocoll. 2009;23:1670-7. https://doi.org/10.1016/j.foodhyd.2009.01.006   DOI
6 Burgain J, Gaiani C, Cailliez-Grimal C, Jeandel C, Scher J. Encapsulation of Lactobacillus rhamnosus GG in microparticles: influence of casein to whey protein ratio on bacterial survival during digestion. Innovative Food Sci Emerging Technol. 2013;19:233-42. https://doi.org/10.1016/j.ifset.2013.04.012   DOI
7 Maciel GM, Chaves KS, Grosso CRF, Gigante ML. Microencapsulation of Lactobacillus acidophilus La-5 by spray-drying using sweet whey and skim milk as encapsulating materials. J Dairy Sci. 2014;97:1991-8. https://doi.org/10.3168/jds.2013-7463   DOI
8 FAO [Food and Agriculture Organization of the United Nations], WHO [World Health Organization]. Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Rome: FAO; 2006. FAO. Food and Nutrition Paper No.: 85.
9 Hill AR, Kethireddipalli P. Dairy products: cheese and yogurt. In: Eskin NAM, Shahidi F, editors. Biochemistry of foods. Amsterdam: Elsevier; 2013. p. 319-62.
10 Shi LE, Li ZH, Li DT, Xu M, Chen HY, Zhang ZL, et al. Encapsulation of probiotic Lactobacillus bulgaricus in alginate-milk microspheres and evaluation of the survival in simulated gastrointestinal conditions. J Food Eng. 2013;117:99-104. https://doi.org/10.1016/j.jfoodeng.2013.02.012   DOI
11 Patel AR. Probiotic fruit and vegetable juices-recent advances and future perspective. Int Food Res J. 2017;24:1850-7.
12 Sheu TY, Marshall RT, Heymann H. Improving survival of culture bacteria in frozen desserts by microentrapment. J Dairy Sci. 1993;76:1902-7. https://doi.org/10.3168/jds.S0022-0302(93)77523-2   DOI
13 Vladisavljevic GT, Tesch S, Schubert H. Preparation of water-in-oil emulsions using microporous polypropylene hollow fibers: influence of some operating parameters on droplet size distribution. Chem Eng Process Process Intensif. 2002;41:231-8. https://doi.org/10.1016/S0255-2701(01)00138-6   DOI
14 Gallotti F, Lavelli V, Turchiuli C. Application of Pleurotus ostreatus β-glucans for oil-in-water emulsions encapsulation in powder. Food Hydrocolloids. 2020;105:105841. https://doi. org/10.1016/j.foodhyd.2020.105841   DOI
15 Fox PF. Influence of temperature and pH on the proteolytic activity of rennet extract. J Dairy Sci. 1969;52:1214-8. https://doi.org/10.3168/jds.S0022-0302(69)86727-5   DOI
16 Rowland IR, Capurso L, Collins K, Cummings J, Delzenne N, Goulet O, et al. Current level of consensus on probiotic science-report of an expert meeting-London, 23 November 2009. Gut Microbes. 2010;1:436-9. https://doi.org/10.4161/gmic.1.6.13610   DOI
17 Zhao M, Huang X, Zhang H, Zhang Y, Ganzle M, Yang N, et al. Probiotic encapsulation in water-in-water emulsion via heteroprotein complex coacervation of type-A gelatin/sodium caseinate. Food Hydrocolloids. 2020;105:105790. https://doi.org/10.1016/j.foodhyd.2020.105790   DOI
18 Anal AK, Singh H. Recent advances in microencapsulation of probiotics for industrial applications and targeted delivery. Trends Food Sci Technol. 2007;18:240-51. https://doi.org/10.1016/j.tifs.2007.01.004   DOI
19 Garcia-Sartal C, Romaris-Hortas V, Barciela-Alonso MC, Moreda-Pineiro A, Dominguez-Gonzalez R, Bermejo-Barrera P. Use of an in vitro digestion method to evaluate the bioaccessibility of arsenic in edible seaweed by inductively coupled plasma-mass spectrometry. Microchem J. 2011;98:91-6. https://doi.org/10.1016/j.microc.2010.12.001   DOI
20 Burgain J, Gaiani C, Linder M, Scher J. Encapsulation of probiotic living cells: from laboratory scale to industrial applications. J Food Eng. 2011;104:467-83. https://doi.org/10.1016/j.jfoodeng.2010.12.031   DOI
21 Ha HK, Rankin SA, Lee MR, Lee WJ. Development and characterization of whey protein-based nano-delivery systems: a review. Molecules. 2019;24:3254. https://doi.org/ 10.3390/molecules24183254   DOI
22 Yonekura L, Sun H, Soukoulis C, Fisk I. Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion. J Funct Foods. 2014;6:205-14. https://doi.org/10.1016/j.jff.2013.10.008   DOI
23 Ranadheera CS, Evans CA, Adams MC, Baines SK. Microencapsulation of Lactobacillus acidophilus LA-5, Bifidobacterium animalis subsp. lactis BB-12 and Propionibacterium jensenii 702 by spray drying in goat's milk. Small Ruminant Res. 2015;123:155-9. https://doi.org/10.1016/j.smallrumres.2014.10.012   DOI
24 Libran CM, Castro S, Lagaron JM. Encapsulation by electrospray coating atomization of probiotic strains. Innov Food Sci Emerg Technol. 2017;39:216-22. https://doi.org/10.1016/j.ifset.2016.12.013   DOI
25 Shalabi SI, Fox PF. Influence of pH on the rennet coagulation of milk. J Dairy Res. 1982;49:153-7. https://doi.org/10.1017/S0022029900022238   DOI
26 FAO [Food and Agriculture Organization of the United Nations], WHO [World Health Organization]. CODEX Standard for fermented milks. Rome: FAO; 2018. Report No.: CXS 243-2003.
27 Champagne CP, Gardner NJ, Roy D. Challenges in the addition of probiotic cultures to foods. Crit Rev Food Sci Nutr. 2005;45:61-84. https://doi.org/10.1080/10408690590900144   DOI
28 Picot A, Lacroix C. Encapsulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal conditions and in yoghurt. Int Dairy J. 2004;14:505-15. https://doi.org/10.1016/j.idairyj.2003.10.008   DOI
29 Horne DS, Lucey JA. Rennet-induced coagulation of milk. In: Fox PF, McSweeney PLH, Cogan TM, Guinee TP, editors. Cheese: chemistry, physics and microbiology. London: Academic Press; 2017. p. 115-43.
30 Chandramouli V, Kailasapathy K, Peiris P, Jones M. An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions. J Microbiol Methods. 2004;56:27-35. https://doi.org/10.1016/j.mimet.2003.09.002   DOI
31 Chew NYK, Chan HK. Effect of powder polydispersity on aerosol generation. J Pharm Pharm Sci. 2002;5:162-8.
32 Abbaszadeh S, Gandomi H, Misaghi A, Bokaei S, Noori N. The effect of alginate and chitosan concentrations on some properties of chitosan-coated alginate beads and survivability of encapsulated Lactobacillus rhamnosus in simulated gastrointestinal conditions and during heat processing. J Sci Food Agric. 2014;94:2210-6. https://doi.org/10.1002/jsfa.6541   DOI
33 Chavarri M, Maranon I, Ares R, Ibanez FC, Marzo F, Villaran MC. Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions. Int J Food Microbiol. 2010;142:185-9. https://doi.org/10.1016/j.ijfoodmicro.2010.06.022   DOI
34 Marshall RJ. An improved method for measurement of the syneresis of curd formed by rennet action on milk. J Dairy Res. 1982;49:329-36. https://doi.org/10.1017/S0022029900022433   DOI