• 미생물학회지
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• 제28권2호
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• pp.169-173
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• 1990

E. coli를 이용하여 L-phenylalanine을 대량 생산하기 위한 재조합 플라스미드 pMW10, pMW11과 pMW 12를 구성하였다. L- Phenylalanine 생산을 위한 유전자 $aroF^{FR}$, $pheA^{FR}$은 E. coli MWEC 101-5 균주로부터 분리하였다. 재조합 플라스미드를 함유하고 있는 E.coli 대사 조절변이주들의 L-phenylalanine 생산생과 안전성을 조사하여 $aroF^{FR}$, $pheA^{FR}$유전자들의 효율을 알아보았다. MWEC 101-5/pMW 11 균주에서는 24.3 g/I의 L-phenylalanine이 생산되었으나, 플라스미드의 안정성은 73.8%였다. 본 균주의 prephemte dehydratase, 고유 활동도는 E. coli K-12에 비하여 26배 증가된 것이다.

• Tuberculosis and Respiratory Diseases
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• 제73권1호
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• pp.11-21
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• 2012

Background: While qualitative analysis of methylation has been reviewed, the quantitative analysis of methylation has rarely been studied. We evaluated the methylation status of CDKN2A, $RAR{\beta}$, and RASSF1A promoter regions in non-small cell lung carcinomas (NSCLCs) by using pyrosequencing. Then, we evaluated the association between methylation at the promoter regions of these tumor suppressor genes and the clinicopathological parameters of the NSCLCs. Methods: We collected tumor tissues from a total of 53 patients with NSCLCs and analyzed the methylation level of the CDKN2A, $RAR{\beta}$, and RASSF1A promoter regions by using pyrosequencing. In addition, we investigated the correlation between the hypermethylation of CDKN2A and the loss of $p16^{INK4A}$ immunoexpression. Results: Hypermethylation of CDKN2A, $RAR{\beta}$, and RASSF1A promoter regions were 16 (30.2%), 22 (41.5%), and 21 tumors (39.6%), respectively. The incidence of hypermethylation at the CDKN2A promoter in the tumors was higher in undifferentiated large cell carcinomas than in other subtypes (p=0.002). Hyperrmethylation of CDKN2A was significantly associated with $p16^{INK4A}$ immunoexpression loss (p=0.045). With regard to the clinicopathological characteristics of NSCLC, certain histopathological subtypes were found to be strongly associated with the loss of $p16^{INK4A}$ immunoexpression (p=0.016). Squamous cell carcinoma and undifferentiated large cell carcinoma showed $p16^{INK4A}$ immunoexpression loss more frequently. The Kaplan-Meier survival curves analysis showed that methylation level and patient survival were barely related to one another. Conclusion: We quantitatively analyzed the promoter methylation status by using pyrosequencing. We showed a significant correlation between CDKN2A hypermethylation and $p16^{INK4A}$ immunoexpression loss.

• 한국표면공학회지
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• 제48권3호
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• pp.73-81
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• 2015

Chloride plating solution was fabricated by dissolving metal Ni powders in solution with HCl and deionized water. Effects of deposition conditions on the properties of Ni films electrodeposited from chloride baths were studied. Current efficiency of Ni films electrodeposited from the baths containing saccharin was decreased with increasing the current density. Residual stress of Ni thin films ware measured to be about 230 ~ 435 MPa in the range of current density of $10{\sim}25mA/cm^2$. Cathode current efficiency in baths without saccharin was initially increased with increasing pH, while it was decreased with increasing pH further. Cathode current efficiency in baths with saccharin (except at pH 2) exhibited less 10 ~ 20% than that in baths without saccharin. Residual stress of Ni films electrodeposited from baths without saccharin was measured to be 388 ~ 473 MPa in the range of pH 2 ~ pH 5 and then was increased to 551 MPa at pH 6. On the other hand, residual stress of Ni films electrodeposited from baths with saccharin was increased with increasing pH. Surface morphology was strongly affected by the change of current density, but slightly by solution pH and addition of saccharin.

• 한국학교ㆍ지역보건교육학회지
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• 제21권1호
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• pp.91-103
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• 2020

Objectives: This study was conducted to investigate the effects of personal characteristics and health beliefs on health behaviors of foreign workers living in Korea. Methods: The subjects of this study were foreign workers who understood the purpose of this study and allowed participation in the workplaces in northern Gyeonggi Province. The final subjects were 206, and collected all data were analyzed by SPSS 23.0. Results: The personal characteristics of the subjects were 51.0% for male and 49.0% for female, and the average age was 31.76(±6.96) years old. The mean for each health belief factors was perceived benefits 4.03(±.56), perceived severity 3.75(±.73), perceived susceptibility 3.65(±.64), self-efficacy 3.56(±.83), and perceived barriers 3.34(±.73). The mean of each health behavior factors was non-smoking 4.02(±.83), drinking 3.92(±.80), health responsibility 3.42(±.83), exercise 3.37(±1.01), dietary habits 1.91(±.11), and stress management 1.72(±.27). The correlation between sub-factors of health belief was all at the level of p <0.01. The sub-factors of health belief were health behavior and perceived susceptibility(r=.773, p<0.01), followed by self-efficacy(r=.760, p<0.01), and perceived severity(r=.574, p<0.01). The factors affecting health behavior were self-efficacy(β=.540, p<.001), perceived susceptibility(β=.461, p<.001), perceived benefits(β=.152, p<.05), marriage status(β=-. 100, p <.05), and income(β=-. 120, p <.05) in order. Conclusion: In-depth consideration of factors that can affect the physical, psychological and social health of foreign workers, and various program development and intervention strategies based on these factors should be sought.

• 자원리싸이클링
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• 제31권3호
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• pp.73-80
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• 2022

폐리튬이온전지를 고온에서 용융환원하면 금속혼합물을 얻을 수 있다. 금속혼합물을 황산이나 염산으로 침출한 다음 용매추출로 분리한 여액에는 니켈(II), 코발트(II), 망간(II)과 실리케이트(IV)가 함유되어 있다. 본 논문에서는 양이온교환수지인 Diphonix와 P204를 사용하여 황산과 염산 합성용액에 함유된 상기 이온들의 이온교환거동을 조사했다. 용액의 pH가 3인 황산용액에서 니켈(II), 코발트(II)와 망간(II)이 선택적으로 Diphonix에 흡착되었다. 용액의 pH가 6인 염산용액에서는 망간(II)이 P204수지에 선택적으로 흡착되어 분리가 가능했다. Diphonix와 P204에 흡착된 금속이온은 황산이나 염산용액으로 세출할 수 있었다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 추계학술대회
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• pp.578-579
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• 2021

중소기업 실태조사에 따르면, 기술보호역량 수준은 매년 나아지고 있으나, 기술유출 및 피해는 지속적으로 발생하고 있다. 이는 중소기업 임직원의 보안의식 강화와 보안수준을 지속적으로 유지할 수 있는 보안관리 및 감독체계가 필요함을 보여준다. 정보보호 및 개인정보보호 관리체계(ISMS-P) 인증 제도가 이와 관련된 최신 기준인데, 이 제도는 ISP, IDC, 병원 및 학교, 중소기업 등 인증 대상 기관의 유형을 고려하지 않고 동일한 인증 기준을 적용하는 문제점이 있다. 본 논문에서는 ISMS-P 인증과 개인정보보호 관리체계(PIMS) 인증을 참고하여 중소기업에 특화되어 적용할 수 있는 73개 평가 항목을 도출하였다. 연구 결과는 기존의 ISMS-P 인증에 비해 평가 항목 수가 28.4% 줄었음을 보여준다.

• 한국수학교육학회지시리즈B:순수및응용수학
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• 제25권2호
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• pp.73-94
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• 2018

We introduce some new type of admissible mappings and prove a coupled coincidence point theorem by using newly defined concepts for generalized compatible pair of mappings satisfying ${\alpha}-{\psi}$ contraction on partially ordered metric spaces. We also prove the uniqueness of a coupled fixed point for such mappings in this setup. Furthermore, we give an example and an application to integral equations to demonstrate the applicability of the obtained results. Our results generalize some recent results in the literature.

• 국제초고층학회논문집
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• 제6권1호
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• pp.73-82
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• 2017

Vertical shortening in tall buildings would be of little concern if all vertical elements shortened evenly. However, vertical elements such as walls and columns may shorten different amounts due to different service axial stress levels. With height, the differential shortening may become significant and impact the strength design and serviceability of the building. Sometimes column transfers or other vertical structural irregularities may cause differential shortening. If differential shortening is not addressed properly, it can impact the serviceability of the building. This paper takes the perspective of a structural engineer in planning the design, predicting the shortening and its effects, and communicating the information to the contractor.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2005년도 춘계학술발표대회 및 제8회 신소재 심포지엄 논문개요집
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• pp.73-73
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• 2005

• Bulletin of the Korean Chemical Society
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• 제8권2호
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• pp.73-79
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• 1987

In this work we have investigated temperature dependence of spin-rotational relaxation rate, $(1/T_1)_{SR}$, of methyl carbon-13's in 2-bromo-p-xylene, 2,5-dimethylaniline, and 2,5-dimethylanisole and have found that temperature behaviors of two methyl carbon-13's in ortho- and meta-position, respectively, are substantially different. It has been confirmed that the modified Burke-Chan model proposed by Park et al. can nicely explain different temperature dependence of $(1/T_1)_{SR}$ for these two methyl carbon-13's while the original Burke-Chan model fails to do so.