• Korean Journal of Ecology and Environment
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• v.35 no.3 s.99
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• pp.198-212
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• 2002

A numerical variation and abnormalities were studied on egg bags and embryos of Korean salamander, Hynobius leechii from agricultural habitat. The teratogenic and toxic effects of fungicide benomyl were also investigated with early embryos from non-agricultural habitat. We collected 144 egg bags from agricultural region, and 3418 of early embryos were contained. The lengths of egg bags were varied from 10 to 23 cm and the most frequent length was 19 cm. The number of embryos was varied from 7 to 43, and the most frequent range was 22 to 26. Spontaneous abnormalities were occurred in 406 embryos among 116 egg bags, and 24 kinds of external abnormalities were found. Individuals showing severe external defect were histologically studied and they showed optic dyspalsia, thyroid carcinoma, somatic muscular dysplasia, partial biaxial structure, decrease of red blood cells in the heart, cephalic degeneration and intestinal dysplasia. 385 embryos from non-agricultural region were exposed to 200 nM${\sim}$ 1 ${\mu}$M of benomyl at blastula or gastrula for 12 days. All embryo were dead in the concentration of 1 ${\mu}$M (LD$_{100}$) and 75% of embryos were dead in 800nM of benomyl. Speciflc effect due to benomyl was acrania or cephalic dysplasia and this restult suggests that the benomyl inhibit stongly to the development of neural tissue. These abnormal developments may be caused by antimitotic action, inhibition of tubulin complex, destruction of microtubule, inhibitions of neurulation and closing of neural fold, and by the inhibition of the movement of neural crest cells.

• Korean Journal of Agricultural Science
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• v.36 no.2
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• pp.135-145
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• 2009

This research was conducted to investigate the effect of various phosphorus concentrations in fertilizer solution on growth of and nutrient uptake by 'Chugyang' egg plant (Solanum melongena L.). Tissue and soil analyses were also conducted to set the threshold levels of phosphorus in plants when disorders develop for phosphorus deficiency or excess. Brown and purple areas developed on the margin of mature leaves and it enlarged rapidly in P deficient plants. The fruits in P deficient plants were small and dull purple in color. When P were excess in fertigation solution, the margins of lower leaves became scorched and it enlarged to inner part of the leaves. The fruits of P excess plants became small and had the curl shape. The tissue $PO_4$-P contents in the most recently fully expanded leaves and dry weight of full above ground plant tissue at 35 days after transplanting showed quadratic response ($y=0.7887+0.2394x-0.0197x^2$) and cubic response ($y=10.43+14.47x-4.7642x^2+0.3977x^3$) to elevated $PO_4$-P concentrations, respectively. When 10% reduction in dry weight set to threshold levels, optimum tissue $PO_4$-P contents are between 0.98 to 1.35%. The yield determined at 150 days after transplanting also showed cubic response to elevated phosphorus concentrations in fertigation solution ($y=1194.6+1502.2x-454.5x^2+35.64x^3$). When the 10% reduction in yield is set to threshold levels, the tissue $PO_4$-P contents for maximum yield should be around 1.53% to 2.25% in most recently fully expanded leaves at 150 days after transplanting.

• Radiation Oncology Journal
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• v.16 no.4
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• pp.425-431
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• 1998

Purpose : We retrospectively analyzed the impact of subpleural lesions of early stage non-small cell lung cancer on the patterns of failure to support selection of postoperative adjuvant therapy. Methods and Materials : The study included 91 patients who underwent surgery for early stage non-small cell lung cancer at Dong-A University Hospital from Dec 1990 to Sep 1996. Twenty five patients were excluded due to postoperative mortality (four patients, 4.4$\%$) and stage III (21 patients). Of 66 patients, 22 patients were subpleural lesions (15 patients in stage I, and seven patients in stage II). Postoperative adjuvant radiation therapy was given to seven patients with T2Nl disease. The median follow-up duration was 29.5 months (range; 8-84 months). Results : The overall survival rate was 69.5$\%$ at 3 years. For all patients who presented with (22 patients) and without (44 patients) subpleural lesions, 3-year overall survival rates were 35.5$\%$ and 84.6$\%$, respectively (p=0.0017). For stage I patients who presented with (15 patients) and without (29 patients) subpleural lesions, 3-year overall survival rates were 33.1$\%$ and 92.3$\%$, respectively (p=0.001). For stage II patients who presented with (7 patients) and without (15 patients) subpleural lesions, 3-year overall survival rates were 53.3$\%$ and 45.7$\%$, respectively (p=0.911). For patients with T2N0 disease (34 patients) who presented with (11 patients) and without (23 patients) subpleural lesions, 3-year overall survival rates were 27.3$\%$ and 90.3$\%$, respectively (p=0.009). Conclusion : These observations suggest that the subpleural lesion play an important role as a prognostic factor for early stage non-small cell lung cancer. Especially for T2N0 disease, patients with subpleural lesions showed significantly lower survival rate than those without that.

• Proceedings of the Mineralogical Society of Korea Conference
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• 1996.10a
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• pp.7-24
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• 1996

'90년대 전반기 중 세계 경제 상황은 정치, 경제 및 환경의 급격한 변화에 따른 불황으로 유황시장에도 심대한 영향을 미치게 되었다. 이와 같은 경기의 후퇴는 다수의 생산자로 하여금 전반적인 시장 상황을 바꿀 수 있는 전략 변경 및 새로운 시장 개발을 촉진시키게 하였다. 최근에 전개되고 있는 극단적인 정치$\cdot$경제적인 변화는 재래시장과 무역균형을 변경시키게 되었다. 개선되는 세계경제에 따른 광범위한 구조조정과 지난 2년간 진행되어온 전반적으로 농업에 유리한 조건에 힘입어 1995년 유황공업은 회복이 시작되어 54.63백만 톤이 생산되었다 국제 유황 무역은 수요와 공급 면에서는 균형을 못 찾고 있다. 1995년 유황생산 중 무역량은 $45\%$에 이르렀다. 유황생산에 영향하는 요소가 변함에 따라 무역의 형태가 변하게 되고 세계 유황 수급 균형에 영향을 주게 되었다. 지난 2년간 있었던 유황생산 회복은 다음 십년간 지속될 것으로 예측된다. 대부분 수요의 증가는 자연가스의 생산을 지배하는 요소에 따라 결정 될 것이다 1986년 이래 유가가 떨어진 후 세계 에너지 수요는 연간 $2.3\%$ 증가하였다. 석유와 가스는 미래 에너지 수요의 증가로 $70\%$이상을 공급하게 될 것이다. 동아시아에서 회수 유황은 원유의 정제로부터 유래된 것으로 가장 신장이 큰 공급원으로 2005년까지 주요유황생산 부분이 될 것이다. 동아시아에서 주요 회수 유황생산국은 일본으로 전체의 $66\%$를 차지한다. 석유 정제로부터 회수 유황생산량은 동아시아에서 증가하고 있다. 유황회수 시설 투자는 일본을 위시하여 한국, 싱가폴 및 태국에서 이루어지고 있으며 이는 점차 증대되는 환경규제에 기인된다. 동아시아 공업국가 예컨대 일본과 한국에서의 유황소비는 인산생산 저조로 정체해 있으나 지난 10년간 여타의 아시아 국가에서의 유황소비는 꾸준히 증가되었다. 이 같은 증가는 앞으로 10년간 계속 될 것으로 추정된다. 이는 유황비료 소비가 4.81백만 톤에서 6.6백만 톤으로 증가될 것으로 예상되고 이는 주로 중국이 내수 인산생산을 증가시키려는 데 기인된다. 더욱이 다가올 10년은 다수의 아시아 국가의 급속한 경제 발전으로 비료 이외의 유황의 소비가 꾸준히 증가 될 것이다. 동아시아는 10.29백만 톤을 생산하고 10.99백만 톤을 소비하여 1995년에는 70만 톤의 유황이 부족하였다. 이와 같은 영향이 계속된다면 동아시아 유황부족은 2005년에는 1.05백만 톤으로 증가될 것으로 예상된다. 그러나 중국에서 황화철에서부터 공급되는 유황함량이 많아 이 지역에서의 원소유황의 진정한 균형에 대해서는 정확하게 평가되지 못한다. 1995년 동아시아에서는 1.3백만 톤의 원소유황을 일본, 캐나다, 미국에서 수입했다. 이들 국가는 앞으로도 이 지역의 주요 공급자가 될 것이다. 황산의 많은 양은 일본에서 이 지역으로 수출되는데 그 양은 1995년 10만톤 이상에 이른다. 더욱이 경제?환경적 이점 때문에 중국이 황화물에서 회수하는 유황대신 원소 유황의 수입을 지속적으로 증가시키고 있어 지역내의 유황 부족이 증가 될 것이다. 이 같은 상황진전으로 앞으로 10년 이내에 2.5백만 톤의 추가시장이 있게 될 것이다. 이 기간내 한국으로서 현재 326,000톤의 부족에서 2005년에는 309,000톤의 과잉으로 유황균형이 변할 수 있는 주요계기가 될 것이다 이 같은 과잉은 회수 유황생산이 1995년 333,000톤에서 2005년 870,000톤으로 $161\%$가 증가될 것으로 예상된다. 동기간 내에 기타 유황생산은 280,000톤에서 320,000톤으로 $14\%$ 증가되는 것으로 추정된다. 그리하여 2005년 한국에서 유황 공급은 1.19백만 톤이고 수요는 881,000톤으로 추정 된다. 미래 한국에서 유황의 또 다른 잠재시장은 식물양분으로서 이다. 인산비료 생산은 유황산업의 골격으로 1995년 세계적으로 인산비료는 유황소비의 53%인 53.60백만 톤을 점유하였다. 작물의 유황결핍 현상은 세계도처에서 나타나고 있어 식물양분으로서 유황시장은 20년전의 시장과 같이 현황을 띠는 시장으로 유황공업이 때를 만나게 될 것이다. 공업국에서 유황의 대기로의 방출억제로 자연 공급량이 감소되고 개발도상국에서 증산으로 유황의 탈취가 증가됨에 따라 유황 부족은 점차 중요한 문제로 확산되고 있다. 세계적으로 $1993\~1994$년간 7.52백만 톤으로 추정되는 유황 부족이 농산물의 수량과 질을 하락시키는 결과를 가져오게 하였다: 이와 같은 현상으로 유황비료의 수요가 증대되었고 산업계는 수요증대에 대응할 기술개발에 노력하게 되었다. 현재의 식량생산과 비료 소비추세가 지속된다면 아시아에서 2000년까지 매년 4.5백만 톤의 유황부족이 있게 될 것이다 이와 같은 유황비료의 부족은 적절한 대책을 취하지 않는 한 2010년에는 6.5백만 톤으로 증가 될 것이다. 동아시아는 경제발전으로 유황비료 장기 잠재시장이 기대되고 새로운 시장으로 $50\%$이상을 점유하게 될 것이다. 서구와 북미에서 유황비료 산업은 이윤 있는 잠재시장으로 인정되고 상업적으로 앞서 있는 시장이다. 점증하는 수요에 대한 대처와 유황비료의 성공은 시장에서 가격에 좌우된다. 실제로 북미와 서구에서 현재의 소매가격은 유황 톤당 $266\~466\$$의 범위에 있다. 인도에서는 비료로서 유황시장은 덜 발달된 단계로서 대표가격은 $120\$$이다. 이 가격 범위로 보아 2010년에 동아시아 시장의 잠재 유황비료 시장은 3.4백만 톤에 이르고 비료공업에서 추가로 얻는 이윤은 408백만 내지 1조5천억$\$$이 될 것이다. 이와 같은 시장이 발전 될 수 있는 것은 계속된 제품개발과 비료산업 시장개척에 달려있다.

• Food Science and Preservation
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• v.14 no.2
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• pp.220-225
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• 2007

This study was performed to measure the antioxidative, antimutagenic, and cytotoxic properties of Prunus armeniaca using the DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical donating method, the Ames test, and cytotoxicity measurements, respectively. Electron-donating abilities were 48.3, 43.9, 14.8 and 12.9 per g dry matter of P. armeniaca seed (PAS), P. armeniaca flesh(PAF), butylated hydroxytoluene, and ${\alpha}-tocopherol$, respectively. The direct antimutagenic effects of an ethanol extract of P. armeniaca were examined in Ames tests using Salmonella typhimurium TA98 and TA100 as reporter organisms. In the Ames test, the ethanol extract of P. armenicaca alone did not exhibit any mutagenicity but the extract did show substantial inhibitory effects against mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 4-nitroquinoline-1-oxide(4NQO). The ethanol extract of PAS(200g dry matter/plate) inhibited strain TA98 mutagenesis induced by 4NQO by ca. 37.9%, and mutation inhibition values of 42.1% and 69.4%, respectively, were observed when 4NQO and MNNG acted on the TA100 strain. The cytotoxic effects of ethanol extracts of P. armeniaca against cell lines of human lung carcinoma(A549), human breast adenocarcinoma(MCF-7), human hepatocellular carcinoma(Hep3B), human cervical adenocarcinoma(HeLa), and human gastric carcinoma(AGS) rose with increases in extract concentration. An ethanol extract(4mg/mL dry matter) of PAF showed strong cytotoxicities of 88.2%, 58%, 72.8%, 89.4%, and 91.9% against A549, AGS, MCF-7, HeLa, and Hep3B cells, respectively. In contrast, the same extract showed only 13 37% cytotoxicity for a nomal human kiney cell line(293). It is suggested that P. armeniaca possesses useful antioxidative, antimutagenic, and anticancer properties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.2
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• pp.79-88
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• 1998

This experiment was conducted from May to August in 1997 to selected the wrn hybrids being suitable for silage at farm in the Kongju National University through the comparison of growth characteristics, forage yield and growth analysis about native and imported corn hybrids for silage production. In this experiment, trial design was a randomized block design with three replication, testing varieties were 4 hybrids (Suwon 19, Kwanganok, Whengsungok, Suwonok ) of native corn hybrids and 13 hybrids (P 3156, P 3352, P 3144w, DK 501, DK 689, DK 713, DK 729, H 643.99, H 545.64, H 645.12, HC 7466, H 644.18, H ALISEO) of imported corn hybrids. The results obtained are summarized as follows; 1. The emergence rate of H643.99 was the highest with 97.0%. In rice black streaked dwarf virus(RBSOV), the hybrid of HC 7466 was lower infected with 1.6% than other hybrids. The plant hight of P 3144w was the highest with 339 cm and the stem length of P 3156 was the highest with 261 cm. In native com hybrids, the plant height and stem length of Kwanganok were recorded with 306 cm and 235 cm, respectively. 2. Leaf number and leaf area of Kwanganok were the greatest with 16 sheet per plant and $5,180\;{\textrm{m}^2}/l0a$, respectively. H 645.12 and H 545.64 had the greatest in ear to total dry matter ratio with 49.5% and 49.4%, respectively. 3. The fresh matter yield was significantly difference between growth stage, So Suwon 19 had the most level at 15 days before silking, P 3352 had the most level at silking date, Kwananok had the most level at 35 days a after silking. The fresh matter yield of native com hybrids such as Suwon 19 and Kwanganok was not apparent diffreences as compared with imported corn hybrids. 4. As the results of survey with dry weight, the quantity of dry matter accumulation were increase after silking. The varieties of P 3352, P 3156, Kwanganok, OK 713 were more quantity of dry matter production than DK 501, HC 7466. The Kwanganok of native com hybrid and Pioneer strain with high percentage of dry matter were higher dry weight than Limagrain strain. 5. HC 7466 had the largest LAR with $6.53\;{\textrm{cm}^2}/g$, H545.12 had the lowest LAR with $3.30\;{\textrm{cm}^2/g}$. P 3144 had the largest LAI, DeKalb strain including DK 713 were larger apparently than Limagrain strain including HC 7466 with 3.15. 6. The RGR of testing varieties was little difference of statistical significantly, but DK 501, and HC 7466 were lower than other corn hybrids. The CGR of native and American varieties was no apparent differences, but that of Limagrain strains were a large variation. According to the results obtained by this experiment, the eary growth such as emergence rate and RBSDV infection rate of Limagrain strains was more excellent than other strains. P 3156, P 3352, P 3144w, DK 713 and HC 7466 were suitable for silage condition such as dry matter yield, percentage of dry matter and % ear to total dry matter. The fresh and dry matter yield of native corn hybrids such as Suwon 19 and Kwanganok were not apparent differences as compared with imported corn hybrids, but percentage of dry matter was lower than other imported corn hybrids.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.2
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• pp.146-154
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• 2002

To assess the adsorption and removal mechanism of dissolved inorganic nutrients in seawater by scattering yellow loess, a laboratory experiment was conducted for the change of nutrient concentration in seawater during the course of time depending on particle size and scattered concentration of the yellow loess. Twenty four hours after the addition of yellow loess in the size range of 0 $\mu$m to 500 $\mu$m in seawater, the removal rate of dissolved inorganic phosphorus (DIP) was increased with increasing amount of yellow loess. There was little difference among the removal rates depending on the size of yellow loess. On average, $26\%$ of dissolved inorganic silicate was reduced for the same period. No greate difference among the removal rate depending on both size and amount of yellow loess was found. Our results suggested that the removal mechanism of DU seemed to be associated with mostly the chemical bond with iron. More than $99\%$ of initial DU concentration was likely to be removed by this mechanism. In the case of inorganic dissolved silicate, the removal mechanism was likely to be attributed to a cation exchange between the yellow loess and seawater.

• Journal of Chest Surgery
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• v.41 no.6
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• pp.687-694
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• 2008

Background: Although aortic valve sclerosis causes no significant hemodynamic alterations, it is associated with an increased risk of cardiovascular death and myocardial infarction. However, the role of ${\beta}_3$ integrin in aortic valve sclerosis remains unclear. Material and Method: Twenty male New Zealand rabbits were divided into two groups. Group 1 rabbits (n=10) received a normal chow diet, while group 2 (n=10) rabbits received a diet containing 1% cholesterol for 12 weeks. After the rabbits were euthanized, their aortic valves and ascending aortas were excised for analysis. Result: Total serum cholesterol ($2,148.3{\pm}1,012.5\;mg/dL$ versus $53.7{\pm}31.8\;mg/dL$, p<0.05), triglyceride ($240.4{\pm}218.3\;mg/dL$ versus $31.6{\pm}6.4\;mg/dL$, p<0.05), and low density lipoprotein (LDL)-cholesterol($2,065.3{\pm}960.9\;mg/dL$ versus $29.1{\pm}30.9\;mg/dL$, p<0.05) levels were significantly higher in the cholesterol diet group compared with the normal diet group. Myofibroblasts and macrophages were more highly expressed in the aortic valve leaflets of rabbits in the cholesterol diet group than of those in the normal diet group. A real-time polymerase chain reaction revealed decreased ${\beta}_3$ integrin mRNA levels in the hypercholesterolemic aortic valves and aortas. Conclusion: The present study shows that hypercholesterolemia induces aortic valve sclerosis. These findings suggest that alterations in ${\beta}_3$ integrin may playa role in the development of aortic valve sclerosis.

• Journal of Hospice and Palliative Care
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• v.4 no.1
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• pp.14-25
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• 2001

Purpose : To determine whether there exist gender differences in pain in Korean cancer patients and whether the depression and performance that are often expressed differently between men and women with cancer interact with pain. Method : The results of survey were collected from 140 in- and out-patients (78 male and 62 female) who had cancer treatment at one of the university hospital in Seoul for four months from February of 1999. The severity and interference of pain were examined with the self-reported survey based on Korean version of Brief Pain Inventory (BPI-K). Demographic and clinical information for all patient were compiled by reviewing their medical records, and the level of depression was examined with the Korean version of Beck Depression Inventory (BDI-K). Usual statistical methods, e.g., frequences, means and SDs were used to characterize the sample. The chi-square tests for categorical data and t-test for numerical data were used for group comparison. And the correlation between variables were performed using Pearson correlation coefficient. Resuts : 1) The mean scores of the worst pain for last 24-hours measured with the pain severity of BPI-K were 5.77 in male and 6.45 in female. The pain interference of BPI-K in men was in the order of mood (5.49), enjoy (5.36), and work (5.00), and in women were work (7.48), enjoy (7.16), and mood (6.53). 2) In pain severity, significant difference was found between men and women in the average pain for last 24-hours (t=-2.130, P=.035). In pain interference, significant difference was found between men and women in activity (t=-2.450, P=.015), mood (t=-2,321, P=.022), walk (t=-2.762, P=.007), work (t=-4.946, P=.000), relate (t=-2.595, P=.010), sleep (t=-2.071, P=.040), enjoy (t=-3.198, P=.001). 3) It was found that the items of pain and depression are significantly correlated in men but not in women. Men also exhibited higher correlation in the items of pain and performance status than women. Conclusions : Women report significantly greater average pain for last 24-hours and for all items of pain interference than men. Pain and depression are significantly correlated in men. The results of this study suggest that gender differences in pain should be considered for planning effective pain management program.

• Journal of Acupuncture Research
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• v.22 no.3
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• pp.169-183
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• 2005

Objective : The purpose of this study was to investigate the anti-caner effect of cobrotoxin on the prostatic cancer cell line (PC-3).The goal of study is to ascertain whether cobrotoxin inhibits tile cell growth and cell cycle of PC-3, or the expression of relative genes and whether the regression of PC-3 cell growth is due to cell death or the expression of gene related to apoptosis. Methods : After the treatment of Pc-3 cells with cobrotoxin, we performed 형광현미경, MTT assay, Western blotting, Flow cytometry, PAGE electrophoresis and Surface plasmon resonance analysis to identify the cell viability, cell death, apoptosis, the changes of cell cycle and the related protein, Adk, MAP kinase. Results : 1. Compared with normal cell, the inhibition of cell growth reduced in proportion with the dose of cobrotoxin(0-16nM) in PC-3. 2. Cell viabilities of 0.1, 1, 4nM cobrotoxin treatment were decreased and those of 8, 16nM were decreased significantly. 3. S phase of cell cycle was decreased at the group of 1, 2, 4, 8, 16nM cobrotoxin, but M phase was increased at 0.1, 1, 2, 4, 8, 16nM cobrotoxin. 4. Cox-2 expression after cobrotoxin was peaked at 12hours and was decreased significantly after 6, 12, 24 hours. 5. The expression of Cdk4 was decreased dose-dependently at 1, 2, 4, 8nM cobrotoxin and was decreased siginificantly at 4, 8nM Cyclin D1 was decreased at 1, 2, 4, 8nM and Cycline E was not changed. Cycline B was decreased at 1, 2, 4, 8nM dose-dependently and was decreased siginificanlty at 2, 4, 8nM. 6. The expression of Akt was decreased at 1, 2, 4, 8nM dose-dependently and was decreased significantly at 2, 4, 8nM. 7. ERK was increased at 1, 2nM and decreased at 4, 8nM, p-ERK was increased at 1, 2, 4 nM, but decreased at 8nM. JNK and p-JNK were increased at 1, 4, 8 nM. p38 was increased at 2nM p-p38 was increased at lnM but decreased significantly at 2, 4, 8nM. 8. The nucli of normal cells were stained round and homogenous in DAPI staining, but those of PC-3 were stained condense and splitted. Apoptosis was increased dose-dependently at 2, 4, 8, 16nM and increased significantly at 2, 4, 8, 16nM. 9. Bax wasn`t changed at 1, 2, 4, 8nM and Bcl-2 was decreased significantly at 1, 2, 4, 8nM. Caspase 3 and 9 weren`t changed at 1, 2, 4nM but were decreased significantly at 8nM. Conclusions : These results indicate that cobrotoxin inhibits the growth of prostate Cancer cells, has anti-cancer effects by inducing apoptosis.