• Asian-Australasian Journal of Animal Sciences
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• v.27 no.8
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• pp.1189-1195
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• 2014

Natural resistance-associated macrophage protein 1 encoding gene (NRAMP1) plays an important role in immune response against intracellular pathogens. To evaluate the effects of NRAMP1 gene on immune capacity in pigs, tissue expression of NRAMP1 mRNA was observed by real time quantitative polymerase chain reaction (PCR), and the results revealed NRAMP1 expressed widely in nine tissues. One single nucleotide polymorphism (SNP) (ENSSSCG00000025058: g.130 C>T) in exon1 and one SNP (ENSSSCG00000025058: g.657 A>G) in intron1 region of porcine NRAMP1 gene were demonstrated by DNA sequencing and PCR-RFLP analysis. A further analysis of SNP genotypes associated with immune traits including contain of white blood cell (WBC), granulocyte, lymphocyte, monocyte (MO), rate of cytotoxin in monocyte (MC) and $CD4^-CD8^+$ T lymphocyte subpopulations in blood was carried out in four pig populations including Large White and three Chinese indigenous breeds (Wannan Black, Huai pig and Wei pig). The results showed that the SNP (ENSSSCG00000025058: g.130 C>T) was significantly associated with level of WBC % (p = 0.031), MO% (p = 0.024), MC% (p = 0.013) and $CD4^-CD8^+$ T lymphocyte (p = 0.023). The other SNP (ENSSSCG00000025058: g.657 A>G) was significantly associated with the level of MO% (p = 0.012), MC% (p = 0.019) and $CD4^-CD8^+$ T lymphocyte (p = 0.037). These results indicate that the NRAMP1 gene can be regarded as a molecular marker for genetic selection of disease susceptibility in pig breeding.

• Journal of Chest Surgery
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• v.46 no.2
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• pp.130-134
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• 2013

Background: Rib fractures are the most common type of thoracic trauma and cause other complications. We explored the risk factors for pneumonia in patients with multiple rib fractures. Materials and Methods: Four hundred and eighteen patients who visited our hospital with multiple rib fractures between January 2002 and December 2008 were retrospectively reviewed. Chest X-rays and chest computed tomography were used to identify injury severity. Patients with only a single rib fracture or who were transferred to another hospital within 2 days were excluded. Results: There were 327 male patients (78%), and the median age was 53 years. The etiologies of the patients' trauma included traffic accidents in 164 cases (39%), falls in 78 cases (19%), slipping and falling in 90 (22%), pedestrian accidents in 30 (7%), industrial accidents in 41 (10%), and assault in 15 (4%). The median number of rib fractures was 4.8. Pulmonary complications including flail chest (2.3%), lung contusion (22%), hemothorax (62%), pneumothorax (31%), and hemopneumothorax (20%) occurred. Chest tubes were inserted into the thoracic cavity in 216 cases (52%), and the median duration of chest tube insertion was 10.26 days. The Injury Severity Score (ISS) and rib score had a median of 15.27 and 6.9, respectively. Pneumonia occurred in 18 cases (4.3%). Of the total cases, 33% of the cases were managed in the intensive care unit (ICU), and the median duration of stay in the ICU was 7.74 days. Antibiotics were administered in 399 patients (95%) for a median of 10.53 days. Antibiotics were used for more than 6 days in 284 patients (68%). The factors affecting pneumonia in patients with multiple rib fractures in multivariate analysis included age (p=0.004), ISS (p<0.001), and rib score (p=0.038). The use of antibiotics was not associated with the occurrence of pneumonia (p=0.28). In-hospital mortality was 5.3% (n=22). Conclusion: The factors affecting risk of pneumonia in patients with multiple rib fractures included age (p=0.004), ISS (p<0.001), and rib score (p=0.038). Elderly patients with multiple traumas have a high risk of pneumonia and should be treated accordingly.

• Preventive Nutrition and Food Science
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• v.8 no.2
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• pp.130-136
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• 2003

The protective effects of onion extract (OE), onion powder extracted in ethanol for 2 days. on carbon tetrachloride ($CCl_4$)-induced hepatotoxicities and the possible mechanisms involved in this protection were investigated in mice. Pretreatment with OE prior to the administration of $CCl_4$ significantly reduced the increase in serum alanine and aspartate aminotransferase activities and hepatic lipid peroxidation in a dose-dependent manner. In addition, pretreatment with OE significantly prevented the depletion of reduced glutathione content in the liver of $CCl_4$-intoxicated mice. $CCl_4$-induced hepatotoxicity was also prevented, as indicated by a liver histopathologic findings. The effects of OE on the cytochrome P450 (P450) 2E1, the major isozyme involved in $CCl_4$ biotransformation were investigated. Treatment of mice with OE resulted in a significant decrease in P450 2E1-dependent p-nitrophenol and aniline hydroxylation in a dose-dependent manner. Consistent with these observations, the P450 2E1 expressions were also decreased, as determined by immunoblot analysis. OE also exhibited antioxidant effects in FeCl$_2$-ascorbate induced lipid peroxidation in rat liver homogenates and in superoxide radical scavenging activity. These results show that the protective effects of OE against the $CCl_4$-induced hepatotoxicity may be due to its ability to block bioactivation of $CCl_4$, mainly tty inhibiting the expression and activities of P450 2E1 and by scavenging free radicals.

• Journal of Korean Society of Forest Science
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• v.97 no.6
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• pp.582-588
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• 2008

This study was carried out to evaluate litterfall amounts in a 28-year old Quercus acutissima stand at various levels of fertilization. The levels of fertilization were control, 3:4:1 (100 kg N/ha, 130 kg P/ha, 33 kg K/ha), 3:8:1, 3:4:2, 6:4:1, and 2:2:1, respectively. Fertilizers were applied for 3 years. Litterfall amounts following 3-year fertilization were significantly different among various levels of fertilization. Leaf litter was significantly higher (P<0.05) in 3:4:1 (4,015 kg/ha/yr) than in 3:8:1 treatments (2,874 kg/ha/yr), whereas other treatments showed no significant differences (P>0.05). Total litterfall amounts throughout the study period were 4,206 kg/ha/yr in 3:8:1, 4,992 kg/ha/yr in 2:2:1, 5,372 kg/ha/yr in 6:4:1, 5,456 kg/ha/yr in control, 5,840 kg/ha/yr in 3:4:1 and 6,015 kg/ha/yr in 3:4:2 treatments, respectively. Proportion of leaf litter was more than 66% of total litterfall in 3:4:1, 6:4:1, and 3:8:1 treatments compared with 61% in the control. These results indicated that litterfall amounts in a Q. accutissma stand were affected by various levels of fertilization.

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.455-460
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• 1986

Dane particle was prepared from the plasma of chronic HBsAg carrier with high levels of HBsAg activity. DNA extracted front Dane particle core after a DNA polymerase reaction with $\alpha$-($^{32}$P) dNTP, was identified as HBV DNA by liquid scintillation counter and agarose gel electrophoresis-G.M. counting. To produce Hepatitis B surface antigen for use as a vaccine against Hepatitis B virus infection, yeast strains harboring recombinant plasmid with Apase promoter was used. Recombinant plasmid was construced from pHBV 130 and pAN 82, transformed into E coli, and then transferred into yeast strains. HBsAg was produced by derepression in Burkholder minimal medium with controlled inorganic phosphate concentration. The kinetics of HBsAg production was also investigated. Total HBsAg activity increased rapidly between 3 and 6 hours after transfer to phosphate-free medium and reached a maximum at around 9th hour. The transfer into phosphate-free medium after 6 hours in high phosphate cell growth medium gave maximum activity.

• Korean Journal of Occupational Health Nursing
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• v.22 no.2
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• pp.130-139
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• 2013

Purpose: This study was to identify the relationship among clinical nurse's job overload, self-efficacy, emotional exhaust and turnover intention. Methods: The study was conducted through a survey on 537 clinical nurses in five general hospitals. The data were collected by self-reporting questionnaires and analysed by the SPSS/WIN 17.0 program using descriptive statistics, t-test, ANOVA, Pearson correlation coefficient and stepwise multiple regression. Results: The mean score of turnover intention was 3.04. Turnover intention according to age, total career, job objective, and work off showed significant differences. The significant positive correlations between emotional exhaust and turnover intention (r=.531, p<.001), job overload and turnover intention (r=.514, p<.001) were found. The significant factors influencing turnover intention were emotional exhaust (${\beta}$=.488, p<.001), career (${\beta}$=.175, p<.001), job overload (${\beta}$=.119, p= .003), and number of night (${\beta}$=.117, p=.002), which accounted for 40.3% of the variance. Conclusion: Thought this result, nursing managers should analyze the reasons for high turnover intention, focusing on the main factors affecting turnover intention and consider the solutions to decrease the turnover intention.

• Journal of Internet of Things and Convergence
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• v.8 no.6
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• pp.75-84
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• 2022

The purpose of this study was to identify the convergence influencing factors on nursing students' awareness of caregiving for the elderly. The participants were 130 nursing students from S University located in W city. Data were collected from July 1 to December 31, 2021, and the collected data were analyzed using the SPSS 25.0 statistical program. As a result of the study, there were statistically significant differences in dementia awareness according to general characteristics in gender, dementia related practice and education experience, and elderly related educational experience, empathy in elderly related practice experience, and the awareness of caregiving for the elderly in dementia interest (Yes). The awareness of caregiving for the elderly showed a positive correlation with empathy (r=.380, p<.01), and the factor effecting the awareness of caregiving for the elderly was dementia interest (yes) (𝛽=.283, p<.001) and empathy (𝛽=.354, p<.001), the explanatory power was 21.8%. Therefore, it is necessary to develop and provide a nursing curriculum or program that can improve empathy ability in order to cultivate nursing students' the awareness of caregiving for the elderly.

• Journal of Digital Convergence
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• v.19 no.7
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• pp.451-461
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• 2021

This study was conducted for nursing students to identify the effects of professors' teaching skills, learners' online learning readiness and major satisfaction on the online learning satisfaction after the COVID-19 pandemic. The participants recruited from nursing colleges located in five province, and data was collected using the Google Online Questionnaire from August 17th to October 5th, 2020. A total of 130 data were analyzed using the SPSS 23.0 program. As a result of this study, the factors that affect online learning satisfaction are the methods in the teaching skills (β=.43, p <.001) and self-directed learning in the online learning readiness (β=.33, p <.001), and satisfaction in nursing major (β=.21, p <.001). The results of this study suggest that education methods need to be devised to enhance the satisfaction of online learning in nursing colleges, and environmental improvements that can be self-directed learning are needed.

• Elastomers and Composites
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• v.39 no.3
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• pp.179-185
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• 2004

Frequency dependency or electrical property stabilization during vulcanization of modified NR/IR composite materials was studied using in-situ electrical property measuring technique. Volume resistivity(p) before and after vulcanization reaction of the sample was measured as the function or frequency in the range or 1Hz to 10kHz at reaction temperatures of 130, 140, 150, and $160^{\circ}C$, respectively. A double stabilization mode of frequency dependency was observed, in which a slow stabilization process of p to a value of ca. $1.0{\times}10^7\;{\Omega}-cm$ occurred after a drastic initial decrease from ca. $9.0{\times}10^7\;{\Omega}-cm$. In addition, notable temperature dependencies of p values were also observed before and after vulcanization reaction, that is, p values at 130 and $140^{\circ}C$ after vulcanization were observed as about 1/3 of those values before vulcanization. All the observed facts were considered as the results from the interaction between the electrode and the bulk sample materials, i.e., electronic charge-discharge, and from the structure change of samples including CB rearrangement by the vulcanization.

• Microbiology and Biotechnology Letters
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• v.34 no.3
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• pp.278-287
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• 2006

Cycloinulooligosaccharide fructanotransferase (CFTase) converts inulin into cycloinulooligosaccharides (cyclofructan, CF) of ${\beta}-(2{\to}1)$-linked D-fructofuranose as well as hydrolysis of cyclofructan. Sequences analysis indicated that CFTase was divided into five distinct regions containing three repeated sequences (R1, R3, and R4) at the N-terminus and C-terminus. Each domain function was investigated by comparison of wild type CFTase enzyme (CFT148) and deletion mutant proteins (CFT108: R1 and R3 deletion; CFT130: R4 deletion; and CFT88: R1, R3, and R4 deletion) of CFTase. The CFT108 mutant had both CFTase and CF hydrolyzing activity as CFT148 did. CFTase activities and CF hydrolysing activities were disappeared in CFT130 and CFT88 mutants. These results indicated that the C-terminal R4 region of P. polymyxa CFTase is necessary for cyclization and hydrolyzing activity.