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Domain Function and Relevant Enzyme Activity of Cycloinulooligosaccharide Fructanotransferase from Paenibacillus polymyxa  

You Dong-Ju (Department of Material and Life science, Graduate School of Engineering, Osaka University)
Park Jung-Ha (Department of Life Science and Biotechnology, Dongeui University)
You Kyung-Ok (Department of Life Science and Biotechnology, Dongeui University)
Nam Soo-Wan (Department of Biotechnology and Bioengineering, Dongeui University)
Kim Kwang-Hyeon (Department of Life Science and Biotechnology, Dongeui University)
Kim Byung-Woo (Department of Life Science and Biotechnology, Dongeui University)
Kwon Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.3, 2006 , pp. 278-287 More about this Journal
Abstract
Cycloinulooligosaccharide fructanotransferase (CFTase) converts inulin into cycloinulooligosaccharides (cyclofructan, CF) of ${\beta}-(2{\to}1)$-linked D-fructofuranose as well as hydrolysis of cyclofructan. Sequences analysis indicated that CFTase was divided into five distinct regions containing three repeated sequences (R1, R3, and R4) at the N-terminus and C-terminus. Each domain function was investigated by comparison of wild type CFTase enzyme (CFT148) and deletion mutant proteins (CFT108: R1 and R3 deletion; CFT130: R4 deletion; and CFT88: R1, R3, and R4 deletion) of CFTase. The CFT108 mutant had both CFTase and CF hydrolyzing activity as CFT148 did. CFTase activities and CF hydrolysing activities were disappeared in CFT130 and CFT88 mutants. These results indicated that the C-terminal R4 region of P. polymyxa CFTase is necessary for cyclization and hydrolyzing activity.
Keywords
Paenibacillus polymyxa; deletion mutant; cyclofructan; repeat region; cycloinulooligosaccharide frctanotransferase;
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1 Eom, S. J., Y. M. Kwon, and Y. J. Choi. 1995. Molecular cloning of Pseudomonas sp. inulinase gene and its expression in E. coli. Kor. J. Appl. Microbiol. Biotechnol. 23: 550-555   과학기술학회마을
2 Jeon, S. J., D. J. You, H. J. Kwon, K. Shigenori, K. Namio, K. H. Kim, Y. H. Kim, and B. W. Kim. 2002. Cloning and characterization of Cycloinulooligosaccharide Fructanotrans-ferase (CFTase) from Bacillus polymyxsa MGL21. J. Microbiol. Biotechnol. 12: 921-928
3 Kanai, T., N. Ueki, T. Kawaguchi, Y. Teranishi, H. Atomi, C. Tomorbaatar, M. Ueda, and A. Tanaka. 1997. Recombinant thermostable cycloinulooligosaccharide fructanotrans- ferase produced by Saccharomyces cerevisiae. Appl. Environ. Microbiol. 63: 4956-4960
4 Kim, D. W., S. J. Lin, S. Morita, I. Terada, and H. Matsuzawa. 1997. A carboxy-terminal prosequence of aqualysin I presents proper folding of the protease domain on its secretion by Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 231: 535-539   DOI   ScienceOn
5 Kim, H. Y. and Y. J. Chio. 2001. Moledular characterization of cycloinulooligosaccharide fructanotransferase(CFTase) from Bacillus macerans. Appl. Environ. Microbiol. 67: 995-1000   DOI   ScienceOn
6 Kubota, M., Y. Matsuura, S. Sakai, and Y. Katsube. 1991. Molecular structure of B. stearothermophilus cyclodextrin glucanotrasferase and analysis of substrate binding site. Denpun Kagaku. 38: 141-146
7 Kushibe, S., K. Mitsui, M. Yamagishi, K. Yamada, and Y. Morimoto. 1995. Purification and characterization of cycloinulooligosaccharide fructanotransferase (CFTase) from Bacillus circulans MCI-2554. Biosci. Biotechnol. Biochem. 59: 31-34
8 Uchiyama, T., M. Kawamura, T. Uragami, and H. Okuno. 1993. Complexing of cycloinulo-oligosaccharides with metal ions. Carbohydr. Res. 241: 245-248   DOI   ScienceOn
9 Kushibe, S., R. Sashida, and Y. Morimoto. 1994. Production of cyclofructan from inulin by Bacillus circulans MCI-2554. Biosci. Biotechnol. Biochem. 58: 1136-1138
10 Kwon, Y. M., H. Y. Kim, and Y. J. Choi. 2000. Cloning and characterization of Pseudomonas mucidolens exoinulinase. J. Microbiol. Biotechnol. 10: 238-243
11 Park, J. H., H. J. Kwon, and B. W. Kim. 2006. High yield production of cyclofructan by deletion mutant enzyme of cycloinulooligosaccharide fructanotransferase. J. Life Sci. 16: 1-5   과학기술학회마을   DOI
12 Schmid, G. 1989. Cyclodextrin glycosyltransferase production: yield enhancement by overexpression of cloned genes. Trends Biotechnol. 7: 244-248   DOI   ScienceOn
13 Klein, C. and G E. Schulz. 1991. Structure of cycledextrin glycosyltransferase refined at $2.0{\AA}$ resolution. J. Mol. Biol. 217: 737-750   DOI
14 Kawamura, M. and T. Uchiyama. 1993. Reaction catalyzed by cycloinulooligosaccha- ride fructanotransferase, Biosci. Biotechnol. Biochem. 57: 343
15 Takai, Y., Y. Okumura, S. Takahashi, M. Sawada, M. Kawamuta, and T. Uchiyama. 1993. A permethylated cyclic fructo-oligosaccharide host that can bind cations in solution. J. Chem. Soc. Chem. Commun. 1: 53-54
16 Lawson, C. L., R. Vanmontfort, B. Strokopytov, H. J. Rozeboom, K. H. Kalk, G. E. Devries, D. Penninga, L. Dijkhuizen, and B. W. Dijkstra. 1994. Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form. J. Mol. Biol. 236: 590-600   DOI   ScienceOn
17 Eder, J., and A. R. Fersht. 1995. Pro-sequence-assisted protein folding. Mol. Microbiol. 16: 609-614   DOI   ScienceOn
18 Kwon, H. J., S. J. Jeon, D. J. You, K. H. Kim, Y. G. Jeong, Y. H. Kim, Y. M. Kim, and B. W. Kim. 2002. Cloning and characterization of exoinulinase from Bacillus polymyxa. Biotechnol. Lett. 25: 155-159
19 Kawamura, M. and T. Uchiyama. 1994. Purification and some properties of cycloinulo- oligosaccharide fructanotrans-ferase from Bacillus circulans OKUMZ31B. Carbohydr. Res. 260: 297-304   DOI   ScienceOn
20 Kawamura, M., T. Uchiyama, T. Kuramoto, Y. Tamura, and K. Mizutani. 1989. Formation of a cycloinulo-oligosaccharide from inulin by an extracellular enzyme of Bacillus circulans OKUMZ31B. Carbohydr. Res. 192: 83-90   DOI   ScienceOn
21 Sawada, M., T. Tanaka, Y. Takai, T. Hanafusa, T. Taniguchi, M. kawamura, and T. Uchiyama. 1991. The crystal structure of cycloinulohexaose produced from inulin by cycloinulooligosaccharide fructanotransferase. Carbohydr. Res. 217: 7-17   DOI   ScienceOn
22 Takata, H., Kuriki, T., Okada, S., Takesada, Y., Iizuka, M., Minamiura, N., Imanaka, T. 1992. Action of neopillilanase. Neopullulanase catalyzes both hydrolysis and transglycosylation at alpha-(1-4)-and alpha-(1-6)-glucosidic linkages. J. Biol. Chem. 267: 18447-18452
23 Wetmore, D. R, S. L, Wong, and R. S. Roche. 1992. The role of the pro-sequence in the processing and secretion of the thermolysin-like neutral protease from Bacillus cereus. Mol. Microbiol. 6: 1593-604   DOI