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Fine mapping of rice bacterial leaf blight resistance loci on K1 and K2 of Korean races of Xoo (Xanthomonas oryzae) using GWAS analysis

  • Hyeon, Do-Yun;Lee, Jeong-Ro;Jo, Gyu-Taek;Raveendar, Sebastin;Sin, Myeong-Jae;Lee, Gyeong-Jun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.62-62
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    • 2019
  • Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1 and K2. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 pairedend read was used for GBS sequencing. GWAS was conducted using TASSEL 5.0. The TASSEL program uses a mixed linear model (MLM). The results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race and K2 resistant germplasm proportion remained between 66.67. The genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than 5 (K1 and K2) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. These SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races. The significant loci detected have also been illustrated and make the CPAS markers for NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

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Comparison of Recognition and Fit Factors according to Education Actual Condition and Employment Type of Small and Medium Enterprises (중소규모 사업장의 교육 환경과 고용형태에 따른 호흡보호구 인식도 및 밀착계수 비교)

  • Eoh, Won Souk;Choi, Youngbo;Shin, Chang Sub
    • Journal of the Korean Society of Safety
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    • v.33 no.6
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    • pp.28-36
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    • 2018
  • There was a difference in recognition of respirators according to the educational performance environment. they were showed higher recognition of respirators of group by internal and external mix trainer, less than 6 months, over 1hour, more than 5 times, variety of education. To identify the relationship between types of job classification(typical and atypical)and the levels of recognition of respirators, a total of 153 workers in a business workplace. mainly, typical workers showed higher recognition of respirators than atypical workers. Training of correct wearing showed high demands both typical and atypical workers. Descriptive statistics(SAS ver 9.2)was performed. the results of recognition of respirators were analyzed the mean and standard deviation by t-test, and anova, fit factor is used geometric means(geometric standard deviation), paired t-test, Wilcoxon analysis(P=0.05). Particulate filtering facepiece respirators (PFFR) is one of the most widely used items of personal protective equipments, and a tight fit of the respirators on the wearers is critical for the protection effectiveness. In order to effectively protect the workers through the respirators, it is important to find and evaluate the ways that can be readily applicable at the workplace to improve the fit of the respirators. This study was designed to evaluate effects of mask style (cup or foldable type) and donning training on fit factors (FF) of the respirators, since these are available at various workplace, especially at small business workplace. A total of 40 study subjects, comprised of employment type workers in metalworking industries, were enrolled in this study. The FF were quantitatively measured before and after training related to the proper donning and use of cup or foldable-type respirators. The pass/fail criterion of FF was set at 100. After the donning training for the cup-type mask, fit test were increased by 769%. but foldable-type mask was also increased after the donning training, the GM of FF for the foldable-type mask and it's increase rate were smaller as compared to the cup-type mask. Furthermore, the differences of the increase rates of the GM of FF in employment type of the subjects were not significantly for the foldable-type mask. These results imply that the raining on the donning and use of PFFR can enhance the protection effectiveness of cup or foldable-type mask, and that the training effects for the foldable-type mask is less significant than that for the cup-type mask. Therefore, it is recommended that the donning training and fit tests should be conducted before the use of the PFFR, and listening to workers opinion regularly.

Seed Quality, Germinability and Initial Growth of Pterocarpus erinaceus (African Rosewood). How Important are Mother Tree Size, Source and Timing of Fruit Harvest?

  • Tiika, Richard J.;Issifu, Hamza;Baatuuwie, Bernard Nuoleyeng;Nasare, Latif Iddrisu;Husseini, Rikiatu
    • Journal of Forest and Environmental Science
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    • v.35 no.2
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    • pp.69-77
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    • 2019
  • Pterocarpus erinaceus is a multipurpose tree species indigenous to semi-arid and Guinean-savanna woodlands of Africa. Natural regeneration on the savanna is being hampered by higher fire frequencies and other land use changes. Simultaneously, demand for timber from the species on the international market is on the rise, raising conservation concerns. To ensure sustainability, good quality seeds, sources of which have not received much research focus, are needed for afforestation. This study investigated how seed quality, germinability and initial seedling growth of P. erinaceus might be influenced by land-use type, mother tree size as well as source and timing of fruit harvest, using both correlational and experimental approaches. The results showed that up to 94.6% of all harvested fruits contained seeds, with no differences found between fruits harvested from cultivated and non-cultivated lands. Percentage of (sound) unblemished seeds was found to be higher for fruits harvested early March (47.3%) than fruits from late April (39.5%). Percentage sound seeds was 41.4% for dispersed fruits (i.e. detached fruits picked from under mother trees) which was not found to differ from undispersed fruits (i.e. fruits harvested while still attached to mother trees) at 45.5%. Also, the influence of fruit harvest time was not found to be different for dispersed and undispersed fruits. Correlations between seed set (proportion of fruits containing seeds) and mother tree size (both tree height and DBH) were found to be very low and non-significant for both dispersed and undispersed fruits. Across mother trees, mean emergence percent was 79.7%, and mean seedling height at three weeks following emergence was 5.32 cm. Both emergence percentage and seedling height were not found to differ among mother trees, but seeds from dispersed fruits had a higher emergence percent (85%) than seeds from undispersed fruits (74%). Implications of findings are discussed.

Translation of Korean Object Case Markers to Mongolian's Suffixes (한국어 목적격조사의 몽골어 격 어미 번역)

  • Setgelkhuu, Khulan;Shin, Joon Choul;Ock, Cheol Young
    • KIPS Transactions on Software and Data Engineering
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    • v.8 no.2
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    • pp.79-88
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    • 2019
  • Machine translation (MT) system, especially Korean-Mongolian MT system, has recently attracted much attention due to its necessary for the globalization generation. Korean and Mongolian have the same sentence structure SOV and the arbitrarily changing of their words order does not change the meaning of sentences due to postpositional particles. The particles that are attached behind words to indicate their grammatical relationship to the clause or make them more specific in meaning. Hence, the particles play an important role in the translation between Korean and Mongolian. However, one Korean particle can be translated into several Mongolian particles. This is a major issue of the Korean-Mongolian MT systems. In this paper, to address this issue, we propose a method to use the combination of UTagger and a Korean-Mongolian particles table. UTagger is a system that can analyze morphologies, tag POS, and disambiguate homographs for Korean texts. The Korean-Mongolian particles table was manually constructed for matching Korean particles with those of Mongolian. The experiment on the test set extracted from the National Institute of Korean Language's Korean-Mongolian Learner's Dictionary shows that our method achieved the accuracy of 88.38% and it improved the result of using only UTagger by 41.48%.

Development of an open source-based APT attack prevention Chrome extension (오픈소스 기반 APT 공격 예방 Chrome extension 개발)

  • Kim, Heeeun;Shon, Taeshik;Kim, Duwon;Han, Gwangseok;Seong, JiHoon
    • Journal of Platform Technology
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    • v.9 no.3
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    • pp.3-17
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    • 2021
  • Advanced persistent threat (APT) attacks are attacks aimed at a particular entity as a set of latent and persistent computer hacking processes. These APT attacks are usually carried out through various methods, including spam mail and disguised banner advertising. The same name is also used for files, since most of them are distributed via spam mail disguised as invoices, shipment documents, and purchase orders. In addition, such Infostealer attacks were the most frequently discovered malicious code in the first week of February 2021. CDR is a 'Content Disarm & Reconstruction' technology that can prevent the risk of malware infection by removing potential security threats from files and recombining them into safe files. Gartner, a global IT advisory organization, recommends CDR as a solution to attacks in the form of attachments. There is a program using CDR techniques released as open source is called 'Dangerzone'. The program supports the extension of most document files, but does not support the extension of HWP files that are widely used in Korea. In addition, Gmail blocks malicious URLs first, but it does not block malicious URLs in mail systems such as Naver and Daum, so malicious URLs can be easily distributed. Based on this problem, we developed a 'Dangerzone' program that supports the HWP extension to prevent APT attacks, and a Chrome extension that performs URL checking in Naver and Daum mail and blocking banner ads.

Current Quality Control Practices of Primary Care Clinics Participating in the National Cancer Screening Program in Korea (의원급 국가암검진기관 질 관리 현황)

  • Lee, Hyewon;Park, Bomi;Han, Kyu-Tae;Her, Eun Young;Jun, Jae Kwan;Choi, Kui Son;Suh, Mina
    • Quality Improvement in Health Care
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    • v.26 no.2
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    • pp.86-94
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    • 2020
  • Purpose: This study aimed to identify current quality control (QC) practices of primary care clinics participating in the National Cancer Screening Program (NCSP) in Korea. Methods: A nationwide survey using a structured questionnaire was conducted among the primary care clinics participating in the NCSP, which were selected by a proportionate stratified sampling. The questionnaire consisted of general information about the responding clinics and the scope of QC activities undertaken. A total of 360 clinics responded and the set of data was then analyzed with Chi-square test and multivariable logistic regression analysis. Results: Among 360 respondents, 332 (92%) reported that they were involved in the QC activities. Most frequently performed QC activities were 'maintenance of facility and instruments' (89%) and 'staff training' (85%). The analysis revealed, with statistical significance (p<.05), that there was an association between certain characteristics of the clinics and the scope of QC activities. These findings also indicated that the diversity of QC practices varies according to the size of the clinics. The clinics screening more types of cancer, those with more screenees, and those with more employees were more likely to implement various QC activities including 'maintenance of facility and instruments', 'external quality control', and 'management of screening data'. Conclusion: To our knowledge, this is the first study to investigate the current status of QC activities conducted among primary care clinics participating in the NCSP. The results of this survey can be used as a basis for further development of policies on quality management of small- and medium-sized primary care clinics in Korea. However, further studies encompassing various aspects of QC activities and management of primary care clinics are needed to assess the current situation in a concise manner.

Standardization and HPTLC Fingerprinting of a Polyherbal Unani Formulation

  • Beg, Mirza Belal;Viquar, Uzma;Naikodi, Mohammad Abdul Rasheed;Suhail, Habiba;Kazmi, Munawwar Husain
    • CELLMED
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    • v.11 no.1
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    • pp.4.1-4.8
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    • 2021
  • Background: The Unani system of medicine has been practised since centuries for the treatment of a range of diseases. In spite of their efficacy they have been widely criticised due to the lack of standardization and poor quality control. Standardization of Unani medicine is a valuable issue at the present because they are very prone to contamination, deterioration, adulteration and variation in composition due to biodiversity as well as careless collection. Objective: To Standardize and Development of HPTLC Fingerprinting of a polyherbal Unani formulation Qurs-e-Safa. Materials and methods: The conventional and modern analytical techniques were used to standardise Qurs-e-Safa. The study was carried into three different batches of Qurs-e-Safa prepared with its ingredients. The parameters studied are organoleptic, microscopic, physicochemical parameters, phytochemical screening, TLC, HPTLC profile, aflatoxin, microbial load and heavy metal analysis. Results and conclusion: Qurṣ-e-Sa'fa is dark yellow in colour and aromatic smell. Uniformity of diameter and weight variation were found to be 13 ± 0, and 524.7 ± 1.72 mg. friability, hardness and disintegration time of all 3 batches were found to be (0.0615 ± 0.004, 0.0885 ± 0.0047 and 0.0725 ± 0.0058), (3.5 ± 0.2886, 3.67 ± 0.1674 and 3.67 ± 0.1674) and (16 to 17 minutes). Extractive value were found to be maximum in distilled water (38.488 ± 0.20, 37.3824 ± 0.38 and 39.8177 ± 0.13) followed by alcohol (27.5406 ± 0.54, 27.5656 ± 0.32 and 26.9229 ± 0.25). Loss of weight on drying, pH, total ash, acid insoluble ash, qualitative test was set in. Phytochemical screening revealed the presence of Carbohydrates, Phenols, Resins, Proteins, Steroids, fixed oil and Flavonoids. The microbial load was found absent and heavy metals were within permissible limits. The data evolved from the study may serve as a reference to validate and also help in the quality control of other finished products in future research.

Identification of Pleurotus ostreatus cultivars with the application of multiplex-simple sequence repeat markers (Multiplex SSR마커를 이용한 느타리(Pleurotus ostreatus) 품종 판별)

  • Choi, Jong In;Jung, Hwa Jin;Na, Kyeong sook;Oh, Min-Ji;Kim, Min-Keun;Ryu, Jae-San
    • Journal of Mushroom
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    • v.19 no.1
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    • pp.76-80
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    • 2021
  • To develop a method for the differentiation of Pleurotus ostratus cultivars, the multiplex-simple sequence repeat (SSR) primer set based on the SSRs obtained from whole genomic DNA sequence analysis was designed with two polymerase chain reaction (PCR) primer sets. These SSR primer sets were employed to distinguish 10 cultivars and strains. Twenty polymorphic markers were selected based on the genotyping results. PCR with each primer produced 1-4 distinct bands ranging in size from 150 to 350 bp, which was within the expected range. However, since a sole SSR marker was unable to detect polymorphisms in every cultivar, multiplex PCRs with composite PCR primer sets were employed. The multiplex primer, "166+115," completely discriminated 12 cultivars and strains with 40 loci, which were 12 more than the simple arithmetic addition of each locus of the primers 115 and 166. These results might be useful to provide an efficient method for the differentiation of P. ostreatus cultivars with separate PCRs for the quality control of spawn and protection of breeders' rights.

Conscious sedation in dentistry: knowledge and practice among dental professionals in Tanzania

  • Sales, Nicco;Sohal, Karpal Singh;Moshy, Jeremiah Robert;Owibingire, Sira Stanslaus;Deoglas, David K;Laizer, Paulo J
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.21 no.6
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    • pp.557-564
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    • 2021
  • Background: Conscious sedation is a useful adjunct in the treatment of patients in dentistry; however, a lack of knowledge among the dental profession regarding sedation is a restricting factor in the practice of dental sedation. Therefore, this study was conducted to assess the knowledge and practice of sedation in dentistry among dental professionals in Tanzania. Methods: This was a cross-sectional study conducted for five months targeting all practicing dental professionals in Tanzania. A modified questionnaire contained 14 questions regarding knowledge about sedative agents and a section on the practice of sedation. The data obtained from this study were coded and entered into a computer program and analyzed using SPSS software version 23.0. The data are presented as frequencies and percentages in tables and charts. Statistical significance was set at P < 0.05. Results: The age range of participants was between 24 and 63 years (mean 36.6 ± 7.7 years). There were 107 men (78.1%), and the male-to-female ratio was 3.6:1. The majority (76.6%) of participants only had an undergraduate dental degree. Thirty-one percent of participants only worked in publicly owned health facilities. Slightly more than half (59.9%) of participants had satisfactory knowledge regarding sedation in dentistry. There was no statistically significant association between the level of sedation-related knowledge and the demographic characteristics of the participants. Only 21.9% reported using sedation in their practice, and the most commonly used sedative drug was diazepam. The reasons for not using dental sedation in clinical practice included a perceived lack of knowledge on sedation, lack of equipment, and cost. Conclusion: Most dental professionals in Tanzania have basic knowledge of sedation in dentistry, although knowledge regarding sedative agents is generally low. The practice of sedation in dentistry in Tanzania is very low compared to that in middle- and high-income countries. Inadequate knowledge, lack of equipment, and the cost of practicing sedation are the main reasons for not practicing sedation.

The Use and Degree of Discomfort of Informatization Device Among the Elderly According to the Disabilities (장애여부에 따른 노인의 정보화기기 사용 및 불편함)

  • Kim, Hee-Young
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.8
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    • pp.327-335
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    • 2021
  • The purpose of this study is to find out the use and inconvenience of informatization devices according to the disability of the elderly. A total 10,097 data from the elderly survey were used to analyze the differences in the possession and use of information devices, usage capabilities, and injustice according to disability. Descriptive analysis was conducted to find out general characteristics and disability-related characteristics. chi-square tests were conducted to find out the difference in informatization and communication device usage ability and information device usage inconvenience according to disability. The p-value was set at 0.001. As a result of the study, the elderly with disabilities in Korea had lower smartphones and tablet PCs than the non-disabled elderly. In terms of daily inconvenience, the elderly with disabilities often responded that they had no experience or did not know about the inconvenience of online reservations for trains/high-speed buses/intercity buses, ordering kiosks at restaurants, using ATMs, or reducing bank stores. Taken together, the ability of the elderly with disabilities in Korea to possess and use informatization devices is very low and they feel more inconvenience than the non-disabled elderly. It is necessary to improve the ability to possess and use informatization devices for the elderly with disabilities in the rapidly progressing aging of the disabled and the informatization of our society.