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http://dx.doi.org/10.14480/JM.2021.19.1.76

Identification of Pleurotus ostreatus cultivars with the application of multiplex-simple sequence repeat markers  

Choi, Jong In (Gyeonggi-do Agricultural Research &Extension Services)
Jung, Hwa Jin (Department of mushroom science, Korea National college of Agriculture and fisheries)
Na, Kyeong sook (Department of mushroom science, Korea National college of Agriculture and fisheries)
Oh, Min-Ji (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Min-Keun (Gyeongsangnam-do Agricultural Research and Extension Services)
Ryu, Jae-San (Department of mushroom science, Korea National college of Agriculture and fisheries)
Publication Information
Journal of Mushroom / v.19, no.1, 2021 , pp. 76-80 More about this Journal
Abstract
To develop a method for the differentiation of Pleurotus ostratus cultivars, the multiplex-simple sequence repeat (SSR) primer set based on the SSRs obtained from whole genomic DNA sequence analysis was designed with two polymerase chain reaction (PCR) primer sets. These SSR primer sets were employed to distinguish 10 cultivars and strains. Twenty polymorphic markers were selected based on the genotyping results. PCR with each primer produced 1-4 distinct bands ranging in size from 150 to 350 bp, which was within the expected range. However, since a sole SSR marker was unable to detect polymorphisms in every cultivar, multiplex PCRs with composite PCR primer sets were employed. The multiplex primer, "166+115," completely discriminated 12 cultivars and strains with 40 loci, which were 12 more than the simple arithmetic addition of each locus of the primers 115 and 166. These results might be useful to provide an efficient method for the differentiation of P. ostreatus cultivars with separate PCRs for the quality control of spawn and protection of breeders' rights.
Keywords
Cultivar; Microsatellite; Molecular marker; Pleurotus ostreatus; Simple sequence repeat (SSR);
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Chang ST, Miles PG. 1989. Edible Mushroom and Their Cultivation. CRC Press, Boca Raton, Florida.
2 Choi DB, Ding JL, Cha W-S. 2007. Homology search of genus Pleurotus using an internal transcribed spacer region. Kor J Chem Eng 24: 408-412.   DOI
3 Choi JI, Ha TM, Jeon DH, Ju YC, Cheong JC. 2013. Characteristics and breeding of a long-term storable oyster mushroom (Pleurotus ostreatus) variety [Gonji-7ho]. J Mushroom 11: 149-153.   DOI
4 Choi JI, Lee YH, Ha TM, Jeon DH, Chi JH, Shin PG. 2015. Characteristics of new mid-high temperature adaptable oyster mushroom variety [Heuktari] for bottle culture. J Mushroom 13: 74-78.   DOI
5 Gonzalez P, Labarere J. 2000. Phylogenetic relationships of Pleurotus species according to the sequence and secondary structure of the mitochondrial small-subunit rRNA V4, V6 and V9 domains. Microbiology 146: 209-221. http://10.0.4.75/00221287-146-1-209.   DOI
6 Lee JW, Han YS, Han CH, Jeong CC. 2011. Characteristics of a new cultivar Pleurotus ostreatus, Hwaseong# 2. J Mushroom 9: 96-100.   DOI
7 Lee JW, Han YS, Cheong JC. 2013. Characteristics of a new cultivar 'Hwaseong 5ho' in Pleurotus ostreatus. J Mushroom 11: 244-248.   DOI
8 Lee KW, Kim MJ, Jeon JO, Kim IJ. 2018. Characteristics and breeding of a new variety 'Sootari' in Pleurotus ostreatus. J Mushroom 16: 180-185.   DOI
9 Mandeel QA, Al-Laith AA, Mohamed SA. 2005. Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World J Microb Biot 21: 601-607.   DOI
10 Oh MJ, Im JH, Shin PG, Oh YL, Jang KY, Kon WS. 2017. Characterization and breeding of a new cultivar Pleurotus ostreatus 'Heuksol'. J Mushroom 15: 129-133.   DOI
11 Park B, Ha BS, Kim MK, Ryu JS. 2016. Characterization of simple sequence repeats in the Pleurotus ostreatus cultivars, "Heuktari" and "Miso". J Mushrooms 14: 174-178.   DOI
12 Park B, Ha BS, Lee SH, Kim MK, Choi JI, Ryu JS. 2019. Variable-number tandem repeat loci-discriminating Pleurotus ostreatus cultivars. Mycoscience 60: 132-135.   DOI
13 Park SS, Woo SM, Park YJ, Jang KY, Kong WS, Yoo YB, Shin PG. 2010. Molecular characterization of Pleurotus eryngii and Pleurotus ferulae using RAPD and mitochondrial microsatellite genes. J Mushrooms 8: 185-185.
14 Patel S, Goyal A. 2012. Recent developments in mushrooms as anti-cancer therapeutics: a review. 3 Biotech, 2: 1-15. https://doi.org/10.1007/s13205-011-0036-2.   DOI
15 Sánchez C. 2010. Cultivation of Pleurotus ostreatus and other edible mushrooms. Appl Microbiol Biotechnol 85: 1321-1337.   DOI
16 Seo KI, Jang KY, Yoo YB, Park SY, Kim KH, Kong WS. 2011. Development of Suhan Strain-specific SCAR marker in Pleurotus ostreatus. Kor J Mycol 39: 31-38.   DOI
17 Seo KI, Jang K-Y, Yoo YB, Park S-Y, Kim K-H, Kong W-S. 2008. Differentiation among commercial strains of Pleurotus spp. based on DNA fingerprinting using universal rice primer (URP). Kor J Mycol 36: 130-137.   DOI
18 Worrall JJ. 1997. Somatic incompatibility in basidiomycetes. Mycologia 89: 24-36.   DOI
19 Sharma S, Madan M. 1993. Microbial protein from leguminous and non-leguminous substrates. Acta Biotechnol 13: 131-139.   DOI
20 Smith D, Devey ME. 1994. Occurrence and inheritance of microsatellites in Pinus radiata. Genome 37: 977-983.   DOI
21 Yoo YB, Seo KI, Kong WS, Jang KY, Shin PG, Park YJ. 2009. Culture characteristics of Pleurotus commercial strains at different temperature. J Mushroom Sci Prod 7: 122-130.