• 원예과학기술지
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• 제29권3호
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• pp.255-259
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• 2011

Polyphenol oxidases (PPO) are copper-containing enzymes responsible for tissue browning in fruits and vegetables including potato, apple and pears. Although these enzymes have been studied for many years, their physiological roles in plants are not yet clear. Therefore, these enzymes need to be purified to characterize further from potato tubers. The classical methods used for the purification of PPO involve several steps. So in this study, we developed a one-step chromatography process for the potato tuber PPO purification. After removal of salts from dissolved ammonium sulfate precipitates of potato tuber extracts using Sephadex-G50 gel filtration, affinity chromatography was carried out on NHS-activated Sepharose 4B using p-aminobenzoic acid as a ligand. The purified enzyme was confirmed by silver staining and a zymogram. The optimum temperature and pH for the purified potato tuber PPO were $15^{\circ}C$ and pH 6.0, respectively. The results obtained in the present study will aid to evaluate PPO from various fruits and vegetables.

• Bulletin of the Korean Chemical Society
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• 제35권6호
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• pp.1697-1702
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• 2014

A novel complex [$Zn(phen)(o-AB)_2$] [phen: 1,10-phenanthroline o-AB: o-aminobenzoic acid] was synthesized and characterized by elemental analysis and X-ray diffraction single-crystal analysis. The crystal crystallizes in monoclinic, space group P2(1)/c with $a=7.6397(6){\AA}$, $b=16.8761(18){\AA}$, $c=17.7713(19){\AA}$, ${\alpha}=90^{\circ}$, ${\beta}=98.9570(10)^{\circ}$, ${\gamma}=90^{\circ}$, $V=2.2633(4)nm^3$, Z = 4, F(000) = 1064, S = 1.058, $Dc=1.520g{\cdot}cm^{-3}$, $R_1=0.0412$, $wR_2=0.0948$, ${\mu}=1.128mm^{-1}$. The Zn(II) is six coordinated by two nitrogen and four oxygen atoms from the 1,10-phenanthroline and o-aminobenzoic acid to furnish a distorted octahedron geometry. The complex exhibits intense fluorescence at room temperature. Theoretical studies of the title complex were carried out by density functional theory (DFT) B3LYP method. CCDC: 898291.

• BMB Reports
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• 제34권4호
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• pp.299-304
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• 2001

The 3-Deoxy-D-arabinoheptulosonate 7-phosphate (DAHP) synthase is the first enzyme of aromatic amino acid-, folic acid-, and phenazine-1-carboxylic acid biosynthetic pathways. DAHP synthase of Bacillus sp. B-6 that produces phenazine-1-carboxylic acid was feedback inhibited by two intermediary metabolites of aromatic amino acid biosynthetic pathways, prephenate and chorismate, but not by other metabolites, such as anthranilic acid, shikimic acid, p-aminobenzoic acid, and 3-hydroxyanthranilic acid. DAHP synthase of Bacillus sp. B-6 was not inhibited by end products, such as aromatic amino acids, folic acid, and phenazine-1-carboxylic acid. The inhibition of DAHP synthase by prephenate and chorismate was non-competitive with respect to erythrose 4-phosphate and phosphoenolpyruvate. Prephenate and chorismate inhibited 50% of the DAHP synthase activity at concentrations of $2{\times}10^{-5}\;M$ and $1.2{\times}10^{-4}\;M$, respectively The synthesis of DAHP synthase of Bacillus sp. B-6 was not repressed by exogenous aromatic amino acids, folic acid, and phenazine 1-carboxylic acid, single or in combinations.

• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.66-73
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• 2015

The pabS gene of Agaricus bisporus 02 encoding a putative PABA synthase was cloned, and then the recombinant protein was expressed in Escherichia coli BL21 under the control of the T7 promoter. The enzyme with an N-terminal GST tag or His tag, designated GST-AbADCS or His-AbADCS, was purified with glutathione Sepharose 4B or Ni Sepharose 6 Fast Flow. The enzyme was an aminodeoxychorismate synthase, and it was necessary to add with an aminodeoxychorismate lyase for synthesizing PABA. AbADCS has maximum activity at a temperature of approximately 25℃ and pH 8.0. Magnesium or manganese ions were necessary for the enzymatic activity. The Michaelis-Menten constant for chorismate was 0.12 mM, and 2.55 mM for glutamine. H₂O₂ did distinct damage on the activity of the enzyme, which could be slightly recovered by Hsp20. Sulfydryl reagents could remarkably promote its activity, suggesting that cysteine residues are essential for catalytic function.

• 미생물학회지
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• 제42권2호
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• pp.131-134
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• 2006

Clavicorona pyxidata DGUM 29005의 균사 생육을 위한 최적 배양조건 및 효소 활성을 조사하였다. 균사 생육을 위한 최적 온도 및 pH는 각각 $24^{\circ}C$ 및 5.0이었다. 사용된 복합배지 중 malt extract medium (MEM)에서 가장 좋은 균사 생육을 나타내었다. 최소배지로 Czapek-Dox 한천배지를 사용하고 탄소원으로 trehalose, mannitol, sucrose 및 maltose 첨가시 균사생육이 우수하였다. 전반적으로 무기질소원이 유기질소원 보다 균사 생육을 더 촉진하였으며, 무기질소원으로 calcium nitrate를 사용하였을 때 균사 생육이 가장 우수하였다. 인산원으로 $Na_2HPO_4$를 사용했을 때 균사생육이 촉진되었으며, 가장 우수한 비타민원은 p-aminobenzoic acid이었다. MEM 액체배지를 사용하여 $24^{\circ}C$에서 20일간 C. pyxidata DGUM 29005를 배양하여 균사외 분비효소 및 균사내 효소의 활성도를 측정한 결과, 균사외 분비 효소 중 laccase의 활성도가 다른 효소에 비해 높았으며, ${\alpha}$-amylase, chitinase, lipase 및 pretense의 활성도는 낮거나 없었다.

• 약학회지
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• 제34권6호
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• pp.422-428
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• 1990

2,3-Dibromo-1,4-naphthoquinone was reacted with p-aminobenzoic acid, 2-aminopyridine, 2-amino-4-metylpyridine, m-nitroaniline, sulfathiazol, p-chloroaniline, phenetidine and 2-bromo-3-(N-arylamino)-1,4-naphthoquinones($1{\sim}8$). 2,3-Epoxy-2,3-dihydro-1,4-naphthoquinone was also reacted with p-amonobenzoic acid, p-toluidine, p-chloroaniline, m-chloroaniline, m-nitroaniline, p-phenetidine, N,N-dimethyl-1,4-pheylenediamine as a ring opening and dehydogenation to form 2-hydroxy-3-(N-arylamino)-naphthoquinones ($9{\sim}16$) in good yield. These new compounds($1{\sim}16$) are expected to have a biological activities such as anticoagulant and cytotoxic.

• 한국식품과학회지
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• 제4권4호
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• pp.239-244
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• 1972

1) 시판(市販) 식용대두유(食用大豆油)를 아크릴수지제(樹脂製) 용기(容器)($28{\times}16{\times}2.5cm$)에 넣어 무색(無色), 적색(赤色), 녹색(綠色) 투명(透明)셀로팬 그리고 자외선흡수제(紫外線吸收劑) 피리딘, 벤조페논, p-아미노벤졸산(酸) 등의 접착제(接着劑) Cemedine C용액(溶液)(10%, 중량비(重量比))을 도포건조(塗布乾燥)시킨 무색투명(無色透明)셀로팬으로 덮고 매일(每日) 일정시간(一定時間)(4.5시간(時間)) 직사일사광선(直射日射光線) 조사(照射)시켜 각종(各種)셀로팬필름의 산화억제효과(酸化抑制效果)를 무색투명(無色透明)셀로팬의 경우를 Control로 하여 비교검토하고, 또한 각시료(各試料)의 산화속도(酸化速度)와 각종(各種) 셀로팬필름의 광선투과율(光線透過率)과의 관계(關係)도 검토하였다. 2) 적색(赤色) 및 녹색(緣色)의 투명(透明)셀로팬은 현저한 산화억제효과(酸化抑制效果)를 보였으며, 양자중(兩者中)에서는 적색투명(赤色透明)셀로팬이 녹색투명(綠色透明)셀로팬에 비해 약간 더 강한 산화억제효과(酸化抑制效果)를 나타냈다. 이는 적색투명(赤色透明)셀로팬이 녹색투명(綠色透明)셀로팬에 비해 단파장영역(短波長領域)의 광선투과율(光線透過率)이 적었기 때문인 것으로 생각된다. 3) 자외선흡수제(紫外線吸收劑)를 도포(塗布)할때 사용한 접착제(接着劑) Cemedine C자체도 상당한 산화억제효과(酸化抑制效果)를 보였었다. 이는 접착제(接着劑)에 의한 얇은 피막형성(被膜形成)으로 control의 무색투명(無色透明)셀로팬보다 각파장(各波長)에서의 광선투과율(光線透過率)이 적었기 때문인 것으로 생각된다. 피리딘, 벤조페논, p-아미노벤졸산(酸)의 Cemedine 용액(溶液)을 처리(處理)한 투명(透明)셀로펜은 무색투명(無色透明)셀로팬에 비하여 적은 광선투과율(光線透過率)을 보였다. 그러 나 피리딘, 벤조페논처리(處理) 투명(透明)셀로팬은 실험초기(實驗初期)에는 무색투명(無色透明)셀로팬과 비교할때 산화억제효과(酸化抑制效果)가 어느정도 인정(認定)되었으나 실험기간(實驗期間)이 각(各) 10일(日), 4일(日)을 경과하면 그 산화억제(酸化抑制) 작용(作用)은 급속(急速)히 상실(喪失)되었다. p-아미노벤졸산처리(酸處理) 투명(透明)셀로팬은 전실험기간(全實驗期間)을 통하여 무색투명(無色透明)셀로팬에 비할 때 현저한 산화억제효과(酸化抑制效果)를 보였다. 4) 결론적(結論的)으로 식용대두유(食用大豆油)의 직사일사광선(直射日射光線)에 의해서 촉진(促進)되는 자동산화(自動酸化)에 대한 이상의 각종(各種) 셀로팬필름의 산화억제효과(酸化抑制效果)의 크기는 다음의 순서였었다. 적색투명(赤色透明) 셀로팬>녹색투명(綠色透明)셀로팬>p-아미노벤졸산처리(酸處理) 투명(透明)셀로팬>Cemedine C단독처리(單獨處理) 투명(透明)셀로팬>무색투명(無色透明)셀로팬>피리딘처리(處理) 투명(透明)셀로팬>벤조페논처리(處理) 투명(透明)셀로팬.

• 대한화장품학회지
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• 제35권3호
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• pp.193-202
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• 2009

본 연구에서는 in vivo 광독성 시험을 대체할 수 있는 방법에 관한 연구를 수행하였다. 인체 유래의 섬유아세포를 활용하여 광독성 물질(promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol)과 비광독성 물질(cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine)을 평가하였다. 세포 생존율은 neutral red uptake (NRU)로 평가하였다. NRU 광독성 시험 결과 bithionol를 제외한 화합물에서 모두 in vivo 실험결과와 유사한 결과를 보였다. 같은 방법으로 화장품 성분인 $Medimin^{(R)}$ A, $Medimin^{(R)}$ D, $LG^{(R)}$ 106W, $Phytoclear^{(R)}$ EL-1, 단삼동 추출물, 미인초 추출물, 산거울 추출물, $Parsol^{(R)}$ MCX와 $Parsol^{(R)}$ 1789를 평가 하였다. 평가 결과 단삼동 추출물을 제외한 원료에서 광독성이 없는 것으로 평가되었다.

• 약학회지
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• 제33권5호
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• pp.273-279
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• 1989

2,3-Dichloro-1,4-naphthoquinone was reacted with o-fluoroaniline, p-sulfadiazine, p-acetoanline, N,N-dimethyl-1,4-phenylenediamine as a nucleophilic substitution to form 2-chloro-3-(N-arylamino)-1,4-naphthoquinones (1.-6.) in good yield. 2,3-Dibromo-1,4-naphthoquinone was also reacted with o-fluoroaniline, m-aminobenzoic acid, m-chloroaniline, morpholine, p-acetoaniline, N,N-dimethyl-1,4-phenylenediamine as a nucleophilic substitution to give 2-bromo-3-(N-arylamino)-1,4-naphthoquinones (7.-12.). These new compounds are expected to have a biological activities such as anticoagulant, cytotoxic.

• 방사선산업학회지
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• 제7권2_3호
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• pp.191-200
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• 2013

In this study, a novel bombesin (BBN) analogues, DOTA-Ala($SO_3H$)-4 aminobenzoyl-Gln-Trp-Ala-Val-Gly-His-Leu-Met-$NH_2$ (DOTA-sBBN) and DOTA-Lys(glucose)-4 aminobenzoyl-Gln-Trp-Ala-Val-Gly-His-Leu-Met-$NH_2$ (DOTA-gluBBN), were synthesized and radiolabeled, and their binding affinities were evaluated. Peptides were prepared by a solid phase synthesis method and their purities were over 98%. DOTA is the chelating agent for $^{177}Lu$-labeling, and the DOTA-conjugated peptides were radiolabeled with $^{177}Lu$ by a high radiolabeling yield (>98%). The Log P values of DOTA-sBBN and DOTA-gluBBN were -2.20 and -2.79, respectively. 50.41% of $^{177}Lu$-DOTA-sBBN and 72.97% of $^{177}Lu$-DOTA-gluBBN were left undegraded by the serum incubation at $37^{\circ}C$ for 48 hr. A competitive displacement of $^{125}I-[Tyr^4]$-BBN on the PC-3 human prostate carcinoma cells revealed that 50% inhibitory concentration ($IC_{50}$) were 1.46 nM of DOTA-sBBN and 4.67 nM of DOTA-gluBBN indicating a highly nanomolar binding affinity for GRPR. Therefore, it is concluded that $^{177}Lu$-DOTA-sBBN and $^{177}Lu$-DOTA-gluBBN can be potential candidates as a targeting modality for the Gastrin-releasing peptide receptor (GRPR)-over-expressing tumors, and further studies to evaluate their biological and pharmacological characteristics are needed.