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Optimized Culture Condition and Enzyme Activity of the Mycelia of Clavicorona pyxidata  

Lee Tae-Hee (Dapartment of Life Science, College of Natural Science, Dongguk University)
Kim Jin-Man (Dapartment of Biology, Graduate School, Dongguk University)
Han Yeong-Hwan (Dapartment of Life Science, College of Natural Science, Dongguk University)
Publication Information
Korean Journal of Microbiology / v.42, no.2, 2006 , pp. 131-134 More about this Journal
Abstract
The culture conditions for the enhanced mycelial of Clavicorona pyxidata DGUM 29005 were investigated. The optimal temperature and pH for the mycelial growth were $24^{\circ}C$ and 5.0, respectively. It was shown that trehalose was the best supplement of carbon sources in Czapek-Dox medium as a minimal medium for enhanced mycelial growth. In general, inorganic nitrogen sources were better than organic ones for mycelial growth. Calcium nitrate was the best out of the inorganic nitrogen test. The appropriate phosphorous and vitamin were $Na_2HPO_4$ and p-aminobenzoic acid, respectively. After the mycelial of C. pyxidata DGUM 29005 was cultivated at $24^{\circ}C$ for 20 days in MEM broth(pH 5.0), the specific activities of both exomycelial and endomycelial enzymes were determined. Among the exomycelial enzyme assayed, the specific activity of laccase was much higher than those of other enzymes. However, little or no enzyme activities of ${\alpha}$-amylase, chitinase, lipase and pretense were found.
Keywords
Clavicorona pyxidata; enzyme; growth; mycelia;
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1 Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254   DOI   ScienceOn
2 Corner, E.J.H. 1970. A monograph of Clavaria and allied Genera. Beih. Nova Hedwigia 33, 1-299
3 Erkel, G. and T. Anke. 1992. Clavicoronic acid, a novel inhibitor of reverse transcriptases from Clavicorona pyxidata (Pers.: Fr.) Doty. J. Anibiotics 45, 29-37   DOI
4 James, S.W. and D.J. McLaughlin. 1988. The influence of carbohydrate source and concentration and light on fruit body development in Clavicorona pyxidata. Mycologia 80, 89-98   DOI
5 Koske, R.E. and C.R. Leathers. 1969. Sporophore production by species of Clavicorona in pure culture. Mycologia 61, 999-1002   DOI
6 Lee, T.H. and Y.H. Han. 2001. Enzyme activities of the fruit body of Ramaria botrytis DGUM 29001. Mycobiology 29, 171-175
7 Miller, O.K. 1977. Mushrooms of North America. E.P. Dutton. Academic Press, New York
8 Lickey, E.B. 2002. Ph.D. thesis. University of Tennessee, Knoxville, Tennessee, USA
9 Choi, S.J., S.J. Kim, and Y.H. Han. 2004. Physiological characteristics and optimized culture conditions of the mycelia of Inonotus mikadoi. Kor. J. Microbiol. 40, 100-103
10 Lee, J.Y. 1988. Coloured Korean Mushrooms. p. 204-213. Academic Publishing Co., Ltd., Seoul
11 Lee, T.H. 2004. Ph.D. thesis. University of Dongguk, Seoul, Korea
12 Dodd, J.L. 1972. The genus Clavicorona. Mycologia 64, 737-773   DOI