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In Vitro Alternative Phototoxicity Test for Various Cosmetic Natural Extracts and Chemicals  

Cho, Wan-Goo (College of Alternative Medicine, Jeonju University)
Park, Jee-Eun (LG Household & Health Care R&D Center)
Park, Mun-Euek (LG Household & Health Care R&D Center)
Lee, Sang-Min (LG Household & Health Care R&D Center)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.35, no.3, 2009 , pp. 193-202 More about this Journal
Abstract
This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including $Medimin^{(R)}$ A, $Medimin^{(R)}$ D, $LG^{(R)}$ 106W, $Phytoclear^{(R)}$ EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, $Parsol^{(R)}$ MCX and $Parsol^{(R)}$ 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.
Keywords
phototoxicity; neutral red uptake; cell viability; fibroblast; cosmetic;
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1 S. Onouea, K. Kawamurac, N. Igarashib, Y. Zhoud, M. Fujikawad, H. Yamadad, Y. Tsudab, Y. Setoa, and S. Yamada, Reactive oxygen species assay- based risk assessment of drug-induced phototoxicity: classification criteria and application to drug candidates, J. Pharm. Biomed. Anal., 47(4 - 5), 967 (2008)   DOI   ScienceOn
2 M. H. Jin, M. H. Jung, Y. H. Lim, S. H. Lee, S. J. Kim, and W. G. Cho, Effect of Angelica${\circledR}$ against photo-aging, J. Soc. Cosmet. Scientists Korea, 31(3), 227 (2005)   ScienceOn
3 S. Onoue, N. Igarashi, S. Yamada, and Y. Tsuda, High-throughput reactive oxygen species (ROS) assay: an enabling technology for screening the phototoxic potential of pharmaceutical substances, J. Pharm. Biomed. Anal., 46, 187 (2008)   DOI   ScienceOn
4 S. N. Park, Active oxygen species, photo-aging and natural extracts, J. Soc. Cosmet. Scientists Korea, 29(1), 117 (2003)
5 K. Kejlova, D. Jurova, and, H. Bendova, Phototoxicity of bergamot oil assessed by in vitro techniques in combination with human patch tests, Toxicology In Vitro, 21(7), 1298 (2007)   DOI   ScienceOn
6 J. K. Lee, D. B. Kim, E. H. Lee, S. R. Ryu, Y. J. Kim, S. S. An, B. M. Lee, Y. S. Lee, and P. Y. Kim, In vitro skin irritation test of humectants with human skin fibroblasts, J. Toxicol. Pub. Health, 15(2), 177 (1999)
7 H. G. Holzhutter and J. Quedeau, Mathematical modeling of cellular responses to external signals, J. Biol. Systems, 3, 127 (1995)   DOI
8 J. K. Lee, E. H. Lee, H. S. Kim, J. T. Hong, S. R. Ryu, K. S. Park, D. B. Kim, and P. Y. Kim, In vitro alternative study of phototoxicity by neutral red uptake assay using human fibroblast, J. Toxicol. Pub. Health, 16(1), 77 (2000)   ScienceOn
9 H. J. Yang, E. H. Kim, and S. N. Park, Antioxidative activity and component analysis of Cayratia japonica extract, J. Soc. Cosmet. Scientists Korea, 34(2), 117 (2008)   ScienceOn
10 Y. Yangal, X. Xiongl, X. Yangl, J. Huanl, X. Tanl, X. Xie, H. S. Zhengl, Q. Lil, and X. He, Establishment and use of 3T3 NRU assay for assessment of phototoxic hazard of cosmetic products, AATEX, 14, Special Issue, 515 (2007)
11 H. G. Holzhutter, A general measure of in vitro phototoxicity derived from pairs of dose-response curves and its use for predicting the in vivo phototoxicity of chemicals, ATLZ, 25, 445 (1997)
12 S. J. Kang, S. D. Hong, W. G. Cho, and Q. Chae, The role of reactive oxygen species on UVA-induced aging of dermal collagen, J. Soc. Cosmet. Scientists Korea, 18(1), 64 (1992)   과학기술학회마을
13 R. Clothier, A. Willshaw, H. Cox, M. Garle, H. Bowler, and R. Combes, The use of human keratinocytes in the EU/COLIPA international in vitro phototoxicity test validation study and the ECVAM/ COLIPA study on UV filter chemicals, ATLA, 27, 247 (1999)   ScienceOn
14 H. Spielmann, W. W. Lovell, E. Holze, B. E. Johnson, T. Maurer, J. W. Wolfgang, W. J. W. Pape, O. Sopora, and D. Sladowski, In vitro phototoxicity testing, ATLA, 22, 314 (1994)   ScienceOn
15 S. T. Chung, H. M. Han, J. H. Kim, P. S. Kang, S. K. Kang, H. S. Kim, and J. I. Kim, Comparison of MTT assay with neutral red uptake assay in measuring phototoxicity in vitro, J. Toxicol. Pub. Health, 14(1), 33 (1998)
16 I. E. Kochevar, Phototoxicity mechanisms: cherpromazine photosensitized demage to DNA and cell membranes, J. Invest. Dermatol., 77, 59 (1981)   DOI   ScienceOn
17 J. Annette, D. O. Margot, B. Drys, and C. A. David, Current status and future developments of databases on alternative methods, ATLA, 25, 411 (1997)   ScienceOn
18 J. H. Epatein and B. U. Wintroub, Photosensitivity due to drugs, Drugs, 30, 42 (1985)   DOI   ScienceOn
19 H. Spielmann, M. Balls, J. Dupuis, W. J. W. Pape, G. Pechovitch, O. De Silva, H. G. Holzhutter, R. Clothier, P. Desolle, F. Gerberick, M. Liebsch, W. W. Lovell, T. Maurer, U. Pfannenbecker, J. M. Potthast, M. Csato, D. Sladowski, W. Steiling, and P. Brantom, The international EU/COLIPA in vitro phototoxicity validation study: results of phase II (blind trial), part 1: the 3T3 NRU phototoxicity test, Toxicology In Vitro, 12, 305 (1998)   DOI   ScienceOn
20 H. Spielmann, S. Hoffmann, M. Liebsch, P. Botham, J. Fentem, C. Eskes, R. Roguet, J. Cotovio, T. Cole, A. Worth, J. Heylings, P. Jones, C. Robles, H. Kandarova, A. Gamer, M. Remmele, R. Curren, H. Raabe, A. Cockshott, I. Gerner, and V. Zuang, The ECVAM international validation study on in vitro tests for acute skin irritation: report on the validity of the EPISKIN and EpiDerm assays and on the skin integrity function test. ATLA, 35, 559 (2007)   ScienceOn
21 H. Spielmann, M. Balls, M. Brand, B. During, H. G. Holzhutter, S. Kalweit, G. Klecak, H. L. Epattenier, M. Liebsch, W. W. Lovell, T. Maurer, F. Moldenhauer, L. Moore, W. J. W. Pape, U. Pfannenbecker, J. Potthast, O. De Silva, W. Steiling, and A. Willshaw, EC/COLIPA project on in vitro phototoxicity testing: first results obtained with the Balb/c 3T3 cell phototoxicity assay, Toxicology In Vitro, 8, 793 (1994)   DOI   ScienceOn