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Protective effects of Korean Red Ginseng against sub-acute immobilization stress-induced testicular damage in experimental rats

  • Lee, Sang-Ho;Choi, Kyung-Hwa;Cha, Kyu-Min;Hwang, Seock-Yeon;Park, Un-Kyu;Jeong, Min-Sik;Hong, Jae-Yup;Han, Chang-Kyun;In, Gyo;Kopalli, Spandana Rajendra;Kim, Si-Kwan
    • Journal of Ginseng Research
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    • v.43 no.1
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    • pp.125-134
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    • 2019
  • Background: Excessive stress causes varied physiological and psychological disorders including male reproductive problems. Here, we attempted to investigate the protective effects of Korean Red Ginseng (Panax ginseng Meyer; KRG) against sub-acute immobilization stress-induced testicular damage in experimental rats. Methods: Male rats (age, 4 wk; weight, 60-70 g) were divided into four groups (n = 8 in each group): normal control group, immobilization control group, immobilization group treated with 100 mg/kg of KRG daily, and immobilization group treated with 200 mg/kg of KRG daily. Normal control and immobilization control groups received vehicle only. KRG (100 mg/kg and 200 mg/kg) was mixed in the standard diet powder and fed daily for 6 mo. Parameters such as organ weight, blood chemistry, sperm kinematic values, and expression levels of testicular-related molecules were measured using commercially available kits, Western blotting, and reverse transcription polymerase chain reaction. Results: Data revealed that KRG restored the altered testis and epididymis weight in immobilization stress-induced rats significantly (p < 0.05). Further, KRG ameliorated the altered blood chemistry and sperm kinematic values when compared with the immobilization control group and attenuated the altered expression levels of spermatogenesis-related proteins (nectin-2, cAMP responsive element binding protein 1, and inhibin-${\alpha}$), sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor), and antioxidant-related enzymes (glutathione S-transferase m5, peroxiredoxin-4, and glutathione peroxidase 4) significantly in the testes of immobilization stress-induced rats. Conclusion: KRG protected immobilization stress-induced testicular damage and fertility factors in rats, thereby indicating its potential in the treatment of stress-related male sterility.

Analysis of Capacitance and Mobility of ZTO with Amorphous Structure (비정질구조의 ZTO 박막에서 커패시턴스와 이동도 분석)

  • Oh, Teresa
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.6
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    • pp.14-18
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    • 2019
  • The conductivity of a semiconductor is primarily determined by the carriers. To achieve higher conductivity, the number of carriers should be high, and an energy trap level is created so that the carriers can cross the forbidden zone with low energy. Carriers have a crystalline binding structure, and interfacial mismatching tends to make them less conductive. In general, high-concentration doping is typically used to increase mobility. However, higher conductivity is also observed in non-orthogonal conjugation structures. In this study, the phenomena of higher conductivity and higher mobility were observed with space charge limiting current due to tunneling phenomena, which are different from trapping phenomena. In an atypical structure, the number of carriers is low, the resistance is high, and the on/off characteristics of capacitances are improved, thus increasing the mobility. ZTO thin film improved the on/off characteristics of capacitances after heat treating at $150^{\circ}C$. In charging and discharging tests, there was a time difference in the charge and discharging shapes, there was no distinction between n and p type, and the bonding structure was amorphous, such as in the depletion layer. The amorphous bonding structure can be seen as a potential barrier, which is also a source of space charge limiting current and causes conduction as a result of tunneling. Thus, increased mobility was observed in the non-structured configuration, and the conductivity increased despite the reduction of carriers.

Effect of Culture Conditions on Pot Cultivation of Veronica longifolia L. (긴산꼬리풀의 분화재배에 미치는 재배조건의 영향)

  • Lee, Sang In;Yeon, Soo Ho;Lee, Seung Yeon;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.86-86
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    • 2019
  • 현삼과(Scrophulariaceae)에 속하는 긴산꼬리풀(Veronica longifolia L.)은 벽자색의 긴 꽃이 총상화서로 달려 청량감을 자아내어 분화소재로 이용한다면 단조로운 실내의 분위기를 환기할 수 있다. 따라서, 조경 및 관상용 식재에 용이한 긴산꼬리풀의 재배는 2018년 5월 15일에 파종하여 생산된 유묘를 2018년 7월 16일에 정식하여 2018년 10월 15일까지 수행하였다. 기비실험은 코트비료(싱싱코트)를 포트당 0, 10 및 20알 처리하였다. 추비는 hyponex (N-P-K, 4-6-6)를 4주 간격으로 총 3회 각 0, 1000 및 $2000mg{\cdot}L-1$로 엽면시비 하였다. 광량 실험은 무차광, 55 및 75% 차광막을 설치하여 수행하였다. 왜화제 종류 및 농도 실험은 dinizonazole(빈나리)과 daminozide (B-9)를 각 0, 1000 및 $2000mg{\cdot}L-1$ 농도로 처리하였으며, 적심처리는 4주차에 1회 처리 유무를 달리하였다. 모든 실험의 공통사항은 플라스틱 10호 화분에 원예상토를 충진한 후 200구 트레이에 종자를 셀당 4립씩 파종하여 생산된 유묘를 정식하였다. 연구의 결과, 기비처리는 초장, 마디수, 엽수가 증가하고, 절간장 및 측지수를 감소시켰으나, 유의적인 차이는 나타나지 않았다. 추비 처리는 농도가 높을수록 엽수가 증가하는 경향을 보였으며, $2000mg{\cdot}L-1$에서 무처리에 비해 유의적으로 많았다. 차광정도에 따른 긴산꼬리풀의 생육은 무차광 조건에서 생육이 가장 양호하였다. 차광률이 증가할수록 전반적인 생육이 억제되고, 개화율도 크게 감소하는 결과를 보였다. 왜화제 처리별로는 종류에 관계없이 전반적인 생육이 억제 되었다. Diniconazole 및 daminozide의 농도가 높을수록 초장이 억제되는 경향을 보였으나, 측지수, 마디수, 엽수도 감소하여 관상적 이용에 적합하지 않다. 적심을 할 경우 전반적인 생육은 큰 차이가 나타나지 않았으며, 측지수가 감소하였다. 따라서, 긴산꼬리풀의 분화생산을 위한 재배기술은 기비는 처리하지 않고, 추비는 $2000mg{\cdot}L-1$로 시비하며, 광이 충분한 곳에서 재배하는 것이 효과적이다. 또한, 왜화제와 적심은 일반적으로 줄기신장을 억제하고 마디수와 엽수를 증가시켜 관상적 이용효과가 있는 것으로 알려져 있지만 본 연구에서는 반대의 결과를 보여 처리 하지 않는 것이 효과적인 것으로 판단된다.

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Several Treatments of Veronica rotunda Nakai for Pot Cultivation (분화 재배를 위한 둥근산꼬리풀의 몇 가지 처리)

  • Yeon, Soo Ho;Lee, Sang In;Lee, Seung Yeon;Lee, Cheol Hee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.89-89
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    • 2019
  • 둥근산꼬리풀(Veronica rotunda Nakai)은 다년생 초본으로 높은산 정상에서 자라며, 초장은 10~20 cm, 잎의 길이는 2~4 cm 정도이다. 또한, 기부의 잎은 엽병이 불분명하고, 줄기에는 털이 없는 것으로 알려져 있다. 관상소재로 이용되고 있으나, 식물정보는 매우 부족하며, 활용하기 위해서는 재배 연구와 식물에 대한 정보 수집이 필요하다. 이에 본 연구는 둥근산꼬리풀의 관상용 재배 시 적정 차광, 기비, 추비, 왜화제 및 적심 등의 처리 정도를 알아보고자 수행하였다. 재배방법의 확립을 위하여 선행연구에서 육묘한 유묘(2018년 5월 15일에 파종하여 생산)를 7월 16일에 정식하여 10월 15일까지 약 14주 동안 재배하였다. 공통조건은 플라스틱 화분 10호(이색포트, $9{\times}9cm$)에 원예상토를 충진하고, 200구 트레이에 셀 당 4립씩 파종하여 생산된 1셀을 정식하였다. 차광 정도 실험은 0, 55, 75% 차광막을 이용하였으며, 기비는 싱싱코트를 0, 10, 20알 처리하였고, 추비는 hyponex (N:P:K, 4:6:6)를 0, 1000, $2000mg{\cdot}L-1$의 농도로 엽면시비 하였다. 또한, 관상효과 증가를 위해 왜화제는 diniconazole, daminozide를 농도별(각 0, 1000, $2000mg{\cdot}L-1$)로 처리하였고, 적심은 4주차에 1회 처리 유무의 효과를 비교하였다. 연구의 결과, 둥근산꼬리풀에 기비 10알 처리는 생육에 유의적인 차이는 보이지 않았으나, 20알 처리는 무처리와 10알 처리에 비해 절간장, 경직경이 증가하는 결과가 나타났다. 추비는 절간장을 증가시켰으나, 측지수와 마디수는 유의적으로 감소시켰다. 차광처리별로는 55, 75% 차광 모두 무차광에 비해 측지수, 마디수, 엽수 및 개화율을 크게 감소시키는 결과를 보여, 둥근산꼬리풀는 광이 충분한 곳에서 재배하는 것이 적합한 것으로 생각된다. 왜화제의 처리는 종류와 농도에 관계없이 둥근산꼬리풀은 측지수, 마디수, 엽수를 감소시키는 결과를 보였다. 또한 초장이 짧아지고 경직경은 두꺼워졌으나, 유의적인 차이는 없었다. 종류별로는 daminozide $2000mg{\cdot}L-1$ 처리는 무처리에 비해 개화율이 증가하여, 둥근산꼬리풀의 분화용 재배시 daminozide $2000mg{\cdot}L-1$를 처리하는 것이 효과적이다. 적심처리는 무처리에 비해 전반적인 생육 및 개화를 억제하는 결과가 나타났다. 결론적으로 둥근산꼬리풀의 분화용 재배시 광량이 충분한 곳에서 재배하고, 기비와 추비는 절간장을 증가시키며, 측지수, 마디수, 엽수에 큰 차이가 없거나 감소하는 결과를 보여 관상용 재배에 적합하지 않은 것으로 판단된다. 추가적으로 daminozide $2000mg{\cdot}L-1$를 처리하며, 적심처리를 하지 않는 것이 좋을 것으로 생각된다.

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Biomass, Nitrogen, and Phosphorus Productivities of Green Manure by Barley and Hairy Vetch Mixtures (보리와 헤어리베치 혼파재배에 따른 녹비작물 수량과 질소와 인산의 생산성)

  • Lee, Cho-Rong;Kim, Pil-Joo;Oh, Yura;Park, Choong-Bae;Park, Kwang-Lai;Nam, Hong-Sik;Park, Gi-Chun
    • Korean Journal of Organic Agriculture
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    • v.26 no.4
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    • pp.719-729
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    • 2018
  • Green manure crops can be efficient replacements of high nutrient materials such as livestock compost, organic fertilizers, etc. in organic farming. Grass-legume mixtures or mixed cropping of legumes with non-legumes can aid in abating the shortcomings of each plant type under monoculture (i.e. legumes have low biomass yields while grasses are poor at fixing nitrogen). This study was conducted to investigate the effects of barley (B) and hairy vetch (H) mixtures on green manure yield in nutrient accumulated organic upland soils of Korea. In one cropping season, single crops of barley and hairy vetch (Barley: 160 kg/ha, Hairy vetch: 90 kg/ha) as well as mixtures of both crops at different seeding rates (B66:H33, B33: H66) were grown and the obtained results are as shown below. The biomass yield and nutrient productivities were higher in barley-hairy vetch mixture. The biomass yield and total phosphorus content were higher for the mixed crops by 78~132% and 200% respectively than those of the hairy vetch monoculture. Total nitrogen content of the mixed crops was also higher than those of the barley monoculture by 43~44%. The biomass yield (5.60 Mg/ha) and nutrient contents (87.7 kg N/ha, 23 kg $P_2O_5/ha$) were highest in the case of B66:H33 seeding rate. Accordingly, this study concludes that the barley-hairy vetch mixtures cropped at B66:H33 seeding rate is efficient in increasing green manure productivity due to complementary effects observed and the highest biomass yield and nutrient contents.

Sequence variation of necdin gene in Bovidae

  • Peters, Sunday O.;Donato, Marcos De;Hussain, Tanveer;Rodulfo, Hectorina;Babar, Masroor E.;Imumorin, Ikhide G.
    • Journal of Animal Science and Technology
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    • v.60 no.12
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    • pp.32.1-32.10
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    • 2018
  • Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

A 90-Day Repeated Oral Dose Toxicity Study of Alismatis Rhizoma Aqueous Extract in Rats

  • Lee, Mu-Jin;Jung, Ho-Kyung;Lee, Ki-Ho;Jang, Ji-Hun;Sim, Mi-Ok;Seong, Tea-Gyeong;Ahn, Byung-Kwan;Shon, Jin-Han;Ham, Seong-Ho;Cho, Hyun-Woo;Kim, Yong-Min;Park, Sung-Jin;Yoon, Ji-Young;Ko, Je-Won;Kim, Jong-Choon
    • Toxicological Research
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    • v.35 no.2
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    • pp.191-200
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    • 2019
  • Alismatis rhizoma (AR), the dried rhizome of Alisma orientale (Sam.) Juzep, is a well-known, traditional medicine that is used for the various biological activities including as a diuretic, to lower cholesterol and as an anti-inflammatory agent. The present study was carried out to investigate the potential toxicity of the Alismatis rhizoma aqueous extract (ARAE) following 90-day repeated oral administration to Sprague-Dawley rats. ARAE was administered orally to male and female rats for 90 days at 0 (control), 500, 1,000 and 2,000 mg/kg/day (n = 10 for male and female rats for each dose). Additional recovery groups from the control group and high dose group were observed for a 28-day recovery period. Chromatograms of ARAE detected main compounds with four peaks. Treatment-related effects including an increase in the red blood cells, hemoglobin, hematocrit, albumin, total protein, and urine volume were observed in males of the 2,000 mg/kg/day group (p < 0.05). However, the diuretic effect of ARAE was considered, a major cause of hematological and serum biochemical changes. The oral no-observed-adverse-effect level (NOAEL) of the ARAE was > 2,000 mg/kg/day in both genders, and no target organs were identified.

Co-firing Characteristics and Slagging Behavior of Sewage Sludge with Coal and Wood Pellet in a Bubbling Fluidized Bed (기포 유동층 반응기를 이용한 하수 슬러지와 석탄 및 우드 펠렛의 혼소 특성 및 슬래깅 성향 연구)

  • Ahn, Hyungjun;Kim, Donghee;Lee, Youngjae
    • Clean Technology
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    • v.24 no.4
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    • pp.323-331
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    • 2018
  • The results of an experimental investigation on the co-firing characteristics and slagging behavior of dried and hydrothermal carbonization sewage sludge, sub-bituminous coal, and wood pellet in a fluidized bed were presented. Combustion tests were conducted in a lab-scale bubbling fluidized bed system at the uniform fuel-air equivalence ratio, air flow rate, and initial bed temperature to measure bed temperature distribution and combustion gas composition. 4 different fuel blending cases were prepared by mixing sewage sludge fuels with coal and wood pellet with the ratio of 50 : 50 by the heating value. $NO_x$ was mostly NO than $NO_2$ and measured in the range of 400 to 600 ppm in all cases. $SO_2$ was considered to be affected mostly by the sulfur content of the sewage sludge fuels. The cases of hydrothermal carbonization sewage sludge mixture showed slightly less $SO_2$ emission but higher fuel-N conversion than the dried sewage sludge mixing cases. The result of fly ash composition analysis implied that the sewage sludge fuels would increase the possibility of slagging/fouling considering the contents of alkali species, such as Na, K, P. Between the two different sewage sludge fuels, dried sewage sludge fuel was expected to have the more severe impact on slagging/fouling behavior than hydrothermal carbonization sewage sludge fuel.

Anti-inflammatory and Anti-bacterial Constituents from the Extracts of Daucus carota var. sativa Aerial Parts (당근 지상부 추출물 유래 항염 및 항균 활성 성분)

  • Kim, Jung Eun;Jo, Yeon Jeong;Lee, Nam Ho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.44 no.4
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    • pp.427-436
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    • 2018
  • In this study, we investigated anti-inflammatory and anti-bacterial constituents from Daucus carota var. sativa (carrot) areal parts. For the extract and solvent fractions, the anti-inflammatory activities were examined by measuring the nitric oxide (NO) production using LPS-stimulated RAW 264.7 cells. Among them, the ethyl acetate (EtOAc) fraction decreased the NO level in a dose-dependent manner. To elucidate further anti-inflammatory mechanisms, EtOAc fraction was evaluated by estimating their effects on the production of prostaglandin $E_2$ and pro-inflammatory cytokines as well as on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). As a result, the EtOAc fraction was determined to inhibit the production of $PGE_2$, IL-$1{\beta}$, IL-6 and reduce the iNOS, COX-2 protein expression. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, n-hexane (Hex) and EtOAc fractions showed the most potent activities. Three phytochemicals were isolated form the EtOAc fraction; diosmetin (1), diosmin (2), cynaroside (3). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including $^1H$ and $^{13}C$ NMR spectra, as well as comparison of the data to the literatures. Anti-inflammatory and anti-bacterial effects were studied for the isolates. All of the compounds (1 - 3) decreased the NO production, effectively. Also, compound 3 showed anti-bacterial activity on P. acnes. Based on these results, D. carota var. sativa extract could be potentially applicable as anti-inflammatory and anti-bacterial ingredients in cosmetic formulations.

Changes in Miscanthus sacchariflorus Growth and Heading Rate Influenced by Water Stress Treatment at Reproductive Growth Stage (생식생장기 수분스트레스 처리가 억새의 출수율 및 생육 변화에 미치는 영향)

  • Lee, Ji-Eun;Cha, Young-Lok;Moon, Youn-Ho;Kim, Kwang-Soo;Kwon, Da-Eun;Kang, Yong-Ku
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.4
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    • pp.390-398
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    • 2018
  • Miscanthus is a perennial energy plant that reproduces via rhizomes and has C4 metabolism. The flowering pattern of a wild type M. sacchariflorus (WTM) is affected by environmental conditions such as photoperiod and soil water status. Geodae-Uksae 1 (Geodea), which is a new M. sacchariflorus cultivar, has a lower heading rate than WMS; however the mechanism with why this happens is unknown. To confirm the effects of drought or waterlogging stresses on the growth of WTM and Geodea at the reproductive stage, we investigated variations in morphological characteristics and nutrient contents of the two Miscanthus species after four months of three water treatments. Morphological traits of the two Miscanthus species under the drought condition were similar to those under the control condition. But, the height of Geodea increased by 30% in response to the waterlogging stress. In WTM, the heading rate under the drought condition was lowest, while there was no significant difference between the waterlogging and control conditions. In the two Miscanthus species, nutrient contents, such as sucrose, total N, $P_2O_5$, K and Mg, were the highest under the drought condition, wherea Ca and Mg contents under the waterlogging condition were more than three times lower than those under the drought condition. The current study results showed that drought stress accelerated senescence and then inhibited nutrient mobilization in WTM, while waterlogging stress promoted the growth. This study is the first report to confirm that waterlogging stress promotes flowering of M. sacchariflorus.