• Clinical and Experimental Reproductive Medicine
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• 제18권1호
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• pp.41-48
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• 1991

The present investigation has been undertaken to elucidate the functional role of ovarian steroids on the mechanism of oviduct differentiation during early embryonic development in rat. The activity of alkaline phosphatase (ALPase) was measured in the oviduct tissue under different steroids treatment regime on day 9 pregnancy. The ALPase activity of the oviduct of pseudopregnant rat was compared with that of normal pregnant rat. The results of day 9 pregnancy rat oviduct clearly demonstrated that $17{\beta}-estradiol$ and progesterone were effective in pseudopregnant rat oviduct. In the ovary intact group the ALPase activity was similar in both of normal and pseudopregnant oviduct, but in the $17{\beta}-estradiol$ treated group the ALPase activity in normal pregnancy was significantly higher than that in pseudopregnancy. The effect of estradiol on the normal pregnant rat oviduct was apparently found on day 3 and day 9 pregnancy. This study, therefore, clearly demonstrates that $17{\beta}-estradiol$ is much potent in oviduct tissue differentiation. It is suggested that absence of $17{\beta}-estradiol$ effect on pseudopregnant rat oviduct is due to there is no embryo passing througth the oviduct.

• 한국발생생물학회지:발생과생식
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• 제24권1호
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• pp.43-52
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• 2020

NUCB2/nesfatin-1 known to regulate appetite and energy homeostasis is expressed not only in the hypothalamus, but also in various organs and tissues. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the reproductive organs, including the ovaries, uterus, and testes of mice. However, it is yet known whether NUCB2/nesfatin-1 is expressed in the oviduct and how its expression is regulated. Therefore, we investigated the expression of NUCB2/nesfatin-1 in the oviduct and its expression is regulated by gonadotropin. Immunohistochemical staining results showed that nesfatin-1 protein was localized in epithelial cells of the oviduct. As a result of quantitative real-time PCR (qRT-PCR) and Western blot, NUCB2/nesfatin-1 was detected strongly in the oviducts. During the estrus cycle, NUCB2/nesfatin-1 expression in the oviducts was markedly higher in the proestrus stage than in other estrus stages. In order to elucidate whether the expression of NUCB2 mRNA is controlled by the gonadotropins, we injected PMSG and hCG and measured NUCB2 mRNA level in the oviduct after injection. Its level was increased in the oviduct after PMSG injection, but no significant change after hCG injection. In addition, NUCB2 mRNA levels were markedly reduced after ovariectomy, while recovered after 17β-estradiol (E2) injection, but not by progesterone (P4). This study demonstrated that NUCB2/nesfatin-1 is highly expressed in the oviduct of mouse and its expression is regulated by E2 secreted by the ovaries. These results suggest that NUCB2/nesfatin-1 expressed by the oviduct may affect the function of the oviduct regulated by the ovaries.

• 대한약리학회지
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• 제13권1호
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• pp.13-21
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• 1977

The author confirmed the development of the smooth muscle in the oviduct proprius and anterior mesosalpinx in the leghorn, and observed that there was a variation between the action of norepinephrine on albumin-secreting portion of productive oviduct and that of non-productive one, and that $PGE_1$ might play a significant role on the activation of adrenergic ${\alpha}$-receptor in the non-productive oviduct. 1. There were many bundles of smooth muscles with irregular directions, which were identified in the both oviduct proprius and anterior mesosalpinx by Mallory aniline-blue orange G stain. 2. In vitro experiments, the anterior mesosalpinx was always relaxed by norepinephrine. While the albumin-secreting portion of non-productive period of oviduct was relaxed, but that of the productive one contracted by norepinephrine. Both the anterior mesosalpinx and oviduct proprius of chick responsed with relaxation to norepinephrine as shown in the non-productive hen. In vivo experiments, norepinephrine injected through the jugular vein increased the intraoviductal pressure in the productive oviduct, but decreased that in the non-productive one. 3. By treatment with $PGE_1$, in vitro, the relaxation induced not only by norepinephrine, but by periarterial electrical stimulation was converted into contraction, and in the presence of phentolamine, this conversion by $PGE_1$ was not shown. 4. The intra-oviductal pressure of the productive hen treated with indomethacin for 4 days was decreased by norepinephrine, but the increase in pressure by $PGE_1$ or $PGE_{2{\alpha}}$ was supersensitized when these drugs were administered through jugular vein. However, in vivo, the relaxation by norepinephrine was not converted into the stimulation after $PGE_1$ treatment. It might be summarized that the regulation of intra-oviductal pressure was dependent on the summation of the movement of both oviduct and mesosalpinx and intramurally produced prostaglandins contributes to the inherent tone of the prcductive oviduct by activating adrenergic ${\alpha}$-receptor.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.531-535
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• 1994

APUD cells in the oviduct of the sheep at standing estrus were localized as paraneurons in the lamina propria sandwiched between this structure and the tunica muscularis by the method of masked metachromasia to toluidine blue after hot mineral acid hydrolysis. These were also confirmed by lead haematoxylin stain and argyrophilia. The oviduct was serialized into 66 zones. Cells were absent in the first and last 2 zones, and most parts of the isthmus. There was however abundant number of APUD cells in the ampulla which were fusiform shaped and were about $5{\mu}m$ width and also in the juncture, where the cells were of a smaller width ($3{\mu}m$) and were quite numerous reaching 180-200 in some zones. It is concluded that peptide secreting cells are numerous in the oviduct and that this may qualify the oviduct as an endocrine organ.

• Asian-Australasian Journal of Animal Sciences
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• 제6권4호
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• pp.469-481
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• 1993

Gametic biotechnologies involve the procedures which are utilized for procuring reproductive success through the mimicry of in vivo events as in in vitro fertilization, embryo transfer etc. With the realization that the oviduct performs most of the procedures mimicked in vitro under normal in vivo situations, the need to master the oviduct therefore, becomes paramount. The oviduct being an exocrine gland (with its output of glycoproteins) and possibly an ecdocrine gland must be implicated in all the preimplantational procedures of reproduction, which include ovulation, oocyte maturation, sperm capacitation, gametic and embryonal nutrition, fertilization, and implantation. The evidences in the literature for the implication of the oviduct in these processes are examined. It is concluded that there is a need for the mastery of oviductual activity in order to maximize the successes of the procedures in vitro, and provide gametic manipulations which will have high success rates in implantation that is the ultimate after of in vitro fertilization for reproductive success.

• 한국임상수의학회지
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• 제9권1호
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• pp.323-332
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• 1992

The present study was carried out to examine the effect of oviduct epithelium and its conditioned medium on e development of early bovine embryos in vitro. Oocytes obtained from ovarian follicles of slaughtered cows were cultured in TCM199 with 10% fetal calf serum for 22-24hrs and then fertillzed in vitro using frozen-thawed semen treated with BO-caffein, BO-BSA(20mM heparin added). Oviduct epithelium was collected in each stage of the estrus cycle and conditioned medium was the medium in which oviduct epithelium in early luteal stage was cultured. In vitro fertilized bovine embryos of 1～2 cell were co-cultured with oviduct epithelium from different estrus cycles, cultured in conditioned medium, and cultured in rabbit oviduct. The cleavage rates of in vitro fertilized early bovine embryos co-cultured with oviduct epithelial cell from early luteal, luteal and follicular phase of estrus cycle(67.2～70.8%) and cultured in conditioned medium(56.7%) were significantly(p<0.05) higher than that of the control(44.2%) The rate of development to morula or blastocyst stage in oviduct epithelial cell co-culture(15.3～32.5%) from three phase of estrus cycles and conditioned medium(14.5%) were significantly(p<0.05) higher than that of the control(5.2%). The oviduct epithelial cell from early luteal phase gave a significantly( p<0.05) higher rate of development to morula or blastocyst stage than both luteal and follicular phase. The results of in vivo culture in rabbit oviduct of early bovine embryos were 52.1% for the cleavage rate and 26.7% for the rate of development to morula or blastocyst stage.

• Molecules and Cells
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• 제19권2호
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• pp.185-190
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• 2005

The chicken oviduct is a dynamic organ that produces secretory proteins such as ovalbumin and its cells undergo cell proliferation and differentiation. There has been no study of the cellular mechanism involved in cell death in the chicken oviduct. Therefore, this study has focused on the study of apoptosis in primary oviduct cells. Because ceramide is known to activate apoptosis in tumor cells and is produced in the oviduct, we used an exogenous ceramide analog to induce cell death. The viability of ceramide-treated chicken oviduct cells decreased in a dose-dependent manner and apoptotic cells were detected by staining with annexin V. The expression of apoptosis-related genes was assessed by RT-PCR and bcl-2 mRNA was found to decrease after exposure to ceramide while Bcl-x mRNA increased 12 h post-treatment. In addition, caspase-3 was expressed strongly in the early stages of apoptosis, while caspase-1 and -9 transcripts increased at later times. We conclude that ceramide induces apoptosis in oviduct-derived primary cells via a caspase- and bcl-2-dependent pathway.

• 한국가금학회지
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• 제29권3호
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• pp.177-183
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• 2002

산란기 한국산 꿩 난관의 술잔세포를 조직화학적으로 분비물의 성상을 관찰하여 다음과 같은 결과를 얻었다. 한국산 꿩 난관 깔때기부의 술잔세포는 황화산성점액물질과 비황화중성점액물을 함유하였다. 난관팽대부, 자궁의 관상샘 개구부, 질의 술잔세포는 황화산성점액물질, 비황화산성점액 물질과 중성점액물질을 함유하였다. 관상샘 개구부의 술잔세포를 제외한 자궁과 난관 좁은 부분의 술잔세포는 중성점액물질을 함유하였다. 한국산 꿩 난관 술잔세포 분비물질의 조직화학적 특징은 난관내의 알의 위치와 연관성이 없었다.

• 한국가금학회지
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• 제26권1호
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• pp.27-33
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• 1999

The distribution of oviductal vessels of eight Korean native pheasants was observed after Latex casts of vessels. Latex was injected into thoracic aorta and posterior vena cava of these birds for cast preparation. The results were as follows: 1. The arteries suppling oviduct of the Korean native pheasants contained A. oviductalis cranialis, media, caudalis and vaginalis. The veins drainaging oviduct were stretched along the following ateries and all of the oviductal vessels were situated on the left side of the body. 2. The anterior oviductal artery arose from the pubic artery of the left external iliac artery and distributed to the infundibulum and the magnum of the oviduct. 3. The middle oviductal artery arose from the left sciatic artery and distributed to the isthmus and shell gland of the oviduct. 4. The caudal artery arose from the left internal pudendal artery of the median sacral artery and distributed to the caudal part of uterus and the cranial part of vagina. 5. The vaginal artery arose from the left internal pudendal artery of median sacral artery and distributed to the caudal part of vagina.

• Applied Microscopy
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• 제30권1호
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• pp.21-44
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• 2000

동면기 (11월부터 3월까지) 동안의 한국산 관박쥐(Rhinolophus ferrumequinum korai) 자성 생식도관 내의 정자저장, 정자 생존 여부 및 정자이동을 알아보기 위하여 전자현미경으로 관찰한 결과는 다음과 같았다. (1) 자궁내강, 자궁선내의 정자들은 다수의 백혈구들에 의해 포식되고 소멸되었다. (2)정자들은 수란관 미측 협부에서만 저장되었고, 정자들의 두부는 상피세포쪽을 향하고 있었다. 이는 교미기(10월 초-중순경)에 사출된 정자들이 긴 동면기 동안에 수란관 미측 협부에서만 생존 가능함을 의미하며, 수란관의 미측 협부가 정자의 수정능 획득(capacitation)에 필요한 최적의 장소임을 의미한다. (3) 동면후기인 3월의 수란관 미측협부에는 정자들이 관찰되지 않았다. 이는 배란기인 4월에 난자를 만나기 위해 정자들이 수정부위인 팽대부 쪽으로 이행하였음을 의미한다. 이상의 결과로 미루어 보아, 긴 동면기 동안의 자성 생식도관 내에서의 정자의 장기저장과 생존여부 및 정자이동은 수정을 위한 일종의 mechanism이라 여겨진다.