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Induction of Apoptosis in Chicken Oviduct Cells by C2-Ceramide  

Kim, Sung Hak (Department of Biology, College of Science, Kyung Hee University)
Choi, Ji Young (Department of Biology, College of Science, Kyung Hee University)
Sihn, Choon-Ryoul (Department of Biology, College of Science, Kyung Hee University)
Suh, Eun Jung (Department of Biology, College of Science, Kyung Hee University)
Kim, Sun Young (School of Agricultural Biotechnology, Seoul National University)
Choi, Kang Duk (Graduate School of Bio and Information Technology, Hankyong National University)
Jeon, Iksoo (Laboratory of Transgenic Chicken, Animal Biotechnology Division, National Livestock Research Institute)
Han, Jae Yong (School of Agricultural Biotechnology, Seoul National University)
Kim, Tae-Yoon (Department of Dermato-Immunology, The Catholic University of Korea)
Kim, Sang Hoon (Department of Biology, College of Science, Kyung Hee University)
Publication Information
Molecules and Cells / v.19, no.2, 2005 , pp. 185-190 More about this Journal
Abstract
The chicken oviduct is a dynamic organ that produces secretory proteins such as ovalbumin and its cells undergo cell proliferation and differentiation. There has been no study of the cellular mechanism involved in cell death in the chicken oviduct. Therefore, this study has focused on the study of apoptosis in primary oviduct cells. Because ceramide is known to activate apoptosis in tumor cells and is produced in the oviduct, we used an exogenous ceramide analog to induce cell death. The viability of ceramide-treated chicken oviduct cells decreased in a dose-dependent manner and apoptotic cells were detected by staining with annexin V. The expression of apoptosis-related genes was assessed by RT-PCR and bcl-2 mRNA was found to decrease after exposure to ceramide while Bcl-x mRNA increased 12 h post-treatment. In addition, caspase-3 was expressed strongly in the early stages of apoptosis, while caspase-1 and -9 transcripts increased at later times. We conclude that ceramide induces apoptosis in oviduct-derived primary cells via a caspase- and bcl-2-dependent pathway.
Keywords
Apoptosis; Ceramide; Chicken Oviduct Cells;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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