• Bulletin of the Korean Chemical Society
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• v.34 no.5
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• pp.1467-1472
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• 2013

Metabolomics is a field that studies systematic dynamics and secretion of metabolites from cells to understand biological pathways based on metabolite changes. The metabolic profiling of intact human colorectal tissues was performed using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy, which was unnecessary to extract metabolites from tissues. We used two different groups of samples, which were defined as normal and cancer, from 9 patients with colorectal cancer and investigated the samples in NMR experiments with a water suppression pulse sequence. We applied target profiling and multivariative statistical analysis to the analyzed 1D NMR spectra to identify the metabolites and discriminate between normal and cancer tissues. Cancer tissue showed higher levels of arginine, betaine, glutamate, lysine, taurine and lower levels of glutamine, hypoxanthine, isoleucine, lactate, methionine, pyruvate, tyrosine relative to normal tissue. In the OPLS-DA (orthogonal partial least square discriminant analysis), the score plot showed good separation between the normal and cancer groups. These results suggest that metabolic profiling of colorectal cancer could provide new biomarkers.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.5C
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• pp.403-412
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• 2008

This paper presents the methods which obtain the solution of Wiener-Hopf equation by LMS algorithm and get the coefficient of TDL filter in lattice filter directly. For this result, we apply an orthogonal input signal generated by lattice filter into an equivalent Wiener-Hopf equation and shows the scheme that can obtain the solution by using the MMSE algorithm. Conventionally, the method like aforementioned scheme can get an error and regression coefficient recursively. However, in this paper, we can obtain an error and the coefficients of TDL filter recursively. And, we make an theoretical analysis on the convergence characteristics of the proposed algorithm. Then we can see that the result is similar to conventional analysis. Also, by computer simulation, we can make sure that the proposed algorithm has an excellent performance.

• Current Optics and Photonics
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• v.3 no.2
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• pp.150-153
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• 2019

Larynx cancer is a potentially terminal and severe type of neck and head cancer in which malignant cells start to grow and spread upwards in the larynx, or voice box. Smoking tobacco, drinking hot beverages and drinking alcohol are the main risk factors for these tumors. In this study, we aimed to develop a precise, accurate and rapid chemometrics assisted Raman spectroscopy method for diagnosis of larynx cancer in deparaffinized tissue samples. In the proposed method, samples were deparaffinized and 20 microns of each tissue were located on a coverslip. Both healthy (n = 13) and cancerous tissues (n = 13) were exposed to a Raman laser (785 nm) and excitations were recorded between wavenumbers of $50{\sim}1500cm^{-1}$. An Orthogonal Partial Least Square algorithm was applied to evaluate the Raman spectrum obtained. Sensitivity and specificity of the proposed method is high enough with the aid of Principal Component Analysis (PCA) to test the whole model. Healthy and cancerous tissues were accurately and precisely clustered. A rapid, easy and precise diagnosis algorithm was developed for larynx cancer. By this method, some useful data about differences in biomolecules of each group (phospholipids, amides, tyrosine, phenylalanine collagen etc.) was also obtained from the spectra. It is claimed that the optimized method has a great potential for clustering and separating tumor tissues from healthy ones. This novel, rapid, precise and objective diagnosis method may be an alternative for the conventional methods in literature for diagnosis of larynx cancer.

• Fisheries and Aquatic Sciences
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• v.1 no.1
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• pp.48-62
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• 1998

Seasonal volume transport on the Texas-Louisiana continental shelf is investigated in terms of objectively fitted transport streamfunction fields based on the current meter data of the Texas­Louisiana Shelf Circulation and Transport Processes Study. Adopted here for the objective mapping is a method employing a two-dimensional truncated Fourier representation of the streamfunction over a domain, with the amplitudes determined by least square fit of the observation. The fitting was done with depth-averaged flow rather than depth-integrated flow to reduce the root-mean-square error. The fitting process filters out $11\%$ of the kinetic energy in the monthly mean transport fields. The shelf-wide pattern of streamfunction fields is similar to that of near-surface velocity fields over the region. The nearshore transport, about 0.1 to 0.3 Sv $(1 Sv= 10^6\;m^3/sec)$, is well correlated with the seasonal signal of along-shelf wind stress. The spring transport is weak compared to other seasons in the inner shelf region. The transport along the shelf break is large and variable. In the southwestern shelf break, transport amounts up to 4.7 Sv, which is associated with the activities of the encroaching of energetic anticyclonic eddies originated in Loop Current of the eastern Gulf of Mexico. The first empirical orthogonal function (EOF) of streamfunction variability contains $67.3\%$ of the variance and shows a simple, shelf-wide, along-shelf pattern of transport. The amplitude evolution of the first EOF is highly correlated (correlation coefficient: 0.88) with the evolution of the along-shelf wind stress. This provides strong evidence that the large portion of seasonal variation of the shelf transport is wind-forced. The second EOF contains $23.7\%$ of the variance and shows eddy activities at the southwestern shelf break. The correlation coefficient between the amplitudes of the second EOF and wind stress is 0.42. We assume that this mode is coupled a periodic inner shelf process with a non-periodic eddy process on the shelf break. The third EOF (accounting for $7.2\% of the variance) shows several cell structures near the shelf break associated with the variability of the Loop Current Eddies. The amplitude time series of the third EOF show little correlation with the along-shelf wind.

• Journal of Distribution Science
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• v.13 no.9
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• pp.103-112
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• 2015

Purpose - This paper aims to provide a step-by-step approach to factor analytic procedures, such as principal component analysis (PCA) and exploratory factor analysis (EFA), and to offer a guideline for factor analysis. Authors have argued that the results of PCA and EFA are substantially similar. Additionally, they assert that PCA is a more appropriate technique for factor analysis because PCA produces easily interpreted results that are likely to be the basis of better decisions. For these reasons, many researchers have used PCA as a technique instead of EFA. However, these techniques are clearly different. PCA should be used for data reduction. On the other hand, EFA has been tailored to identify any underlying factor structure, a set of measured variables that cause the manifest variables to covary. Thus, it is needed for a guideline and for procedures to use in factor analysis. To date, however, these two techniques have been indiscriminately misused. Research design, data, and methodology - This research conducted a literature review. For this, we summarized the meaningful and consistent arguments and drew up guidelines and suggested procedures for rigorous EFA. Results - PCA can be used instead of common factor analysis when all measured variables have high communality. However, common factor analysis is recommended for EFA. First, researchers should evaluate the sample size and check for sampling adequacy before conducting factor analysis. If these conditions are not satisfied, then the next steps cannot be followed. Sample size must be at least 100 with communality above 0.5 and a minimum subject to item ratio of at least 5:1, with a minimum of five items in EFA. Next, Bartlett's sphericity test and the Kaiser-Mayer-Olkin (KMO) measure should be assessed for sampling adequacy. The chi-square value for Bartlett's test should be significant. In addition, a KMO of more than 0.8 is recommended. The next step is to conduct a factor analysis. The analysis is composed of three stages. The first stage determines a rotation technique. Generally, ML or PAF will suggest to researchers the best results. Selection of one of the two techniques heavily hinges on data normality. ML requires normally distributed data; on the other hand, PAF does not. The second step is associated with determining the number of factors to retain in the EFA. The best way to determine the number of factors to retain is to apply three methods including eigenvalues greater than 1.0, the scree plot test, and the variance extracted. The last step is to select one of two rotation methods: orthogonal or oblique. If the research suggests some variables that are correlated to each other, then the oblique method should be selected for factor rotation because the method assumes all factors are correlated in the research. If not, the orthogonal method is possible for factor rotation. Conclusions - Recommendations are offered for the best factor analytic practice for empirical research.

• Journal of Advanced Navigation Technology
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• v.6 no.1
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• pp.37-43
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• 2002

This paper describes the performance evaluation and analysis of Wireless Local Area Network (W-LAN) in the 5 GHz ISM-band in compliance with IEEE 802.11a. At present, most W-LAN products are based on 2.4 GHz band, but low speed (11Mbps) has the limitation to serve systems demanding high-speed data transmission. To solve this problem, it is necessary to design next generation W-LAN system with 54Mbps in the 5GHz. It is sure that implementation of next generation W-LAN will bring competitive advantages. In particular, it will support telecommunications for high-speed mobile environments as well as for fixed places such as a school zone, a lecture room, a hospital and other premises. A few simulation methods are applied to more accurate and reliable performance analysis of next generation W-LAN. To verify if continuous data service is supported for a high-speed mobile notebook, multi-path fading channels between wireless Access Point (AP) and wireless Network Interface Card (NIC) are modeled. In addition, low interference is analyzed via convolutional codes and Orthogonal Frequency-Division Multiplexing (OFDM). Also, to obtain reliable Bit Error Rate (BER), a single tap Least Mean Square (LMS) equalizer is applied. Given the above simulation, next generation W-LAN is an ideal solution for continuous data transmission in high-speed mobile environments.

• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.217-226
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• 2015

Metabolite profiles of seven commercial vinegars and two traditional vinegars were performed by gas chromatography time-of-flight mass spectrometry with multivariate statistical analysis. During alcohol fermentation, yeast, nuruk, and koji were used as sugars for nutrients and as fermentation substrates. Commercial and traditional vinegars were significantly separated in the principal component analysis and orthogonal partial least square discriminant analysis. Six sugars and sugar alcohols, three organic acids, and two other components were selected as different metabolites. Target analysis by ultra-performance liquid chromatography quadruple-time-of-flight mass spectrometry and liquid chromatography-ion trap-mass spectrometry/mass spectrometry were used to detect several metabolites having antioxidant activity, such as cyanidin-3-xylosylrutinoside, cyanidin-3-rutinoside, and quercetin, which were mainly detected in Rural Korean Black raspberry vinegar (RKB). These metabolites contributed to the highest antioxidant activity measured in RKB among the nine vinegars. This study revealed that MS-based metabolite profiling was useful in helping to understand the metabolite differences between commercial and traditional vinegars and to evaluate the association between active compounds of vinegar and antioxidant activity.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.2
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• pp.175-180
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• 2002

In this paper, the system performace with the convolution rode using a Viterbi decoding and the one tap LMS(Least Meam Square) equalizer applied to the OFDM(Orthogonal Frequency Division Multiplexing) system, is analyzed through computer simulation. DMRS(Digital Microwave Radio System)is modeled as Rummler fading channel. In Simulation result, we known that the coding system improved about 3.6dB~10.5dB when BER is 10 $^3$and b is 0.1~0.2 in case of 16QAM(Qurdrature Amplitude Modulation). Also, we known that was improved about 19.7dB when the b is 0.1 and was demanded about 10.5dB when the b is 0.2 in case of 64QAM. we known that the soft decision improved about 2~0.9dB when the b is 0.1~0.2 in case of 16QAM and about 3.3~7.8dB in case of 64QAM. In the equalizer system, efficiency improved from the case of that Eb/No is more than 13dB.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.6A
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• pp.676-684
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• 2008

In digital video broadcasting-terrestrial (DVB-T), which is the European digital terrestrial television standard, the orthogonal frequency division multiplexing (OFDM) has been adopted for signal transmission. The main reasons using OFDM are to increase the robustness against the frequency selective fading and impulse noise, and to use available bandwidth efficiently. However, channel variation within an OFDM symbol destroys orthogonality between subcarriers, resulting in inter-carrier interference (ICI), which increases an error floor in proportional to maximum Doppler spread. This paper provides an ICI analysis in both time and frequency domains while existing literatures analyze the ICI effects mainly in frequency domain and proposes the algorithms that estimate the channel impulse response and channel variation using least square (LS) algorithm which is the most simple channel estimation technique. And we propose adaptive channel estimation algorithm that estimates the velocity of terminals. The simulation results show that proposed algorithm has similar performance with about 1.5% computational complexity of noise and ICI reduction LS algorithm in low speed environments.

• Toxicological Research
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• v.34 no.3
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• pp.199-210
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• 2018

Skeletal muscle can be ultrastructurally damaged by eccentric exercise, and the damage causes metabolic disruption in muscle. This study aimed to determine changes in the metabolomic patterns in urine and metabolomic markers in muscle damage after eccentric exercise. Five men and 6 women aged 19~23 years performed 30 min of the bench step exercise at 70 steps per min at a determined step height of 110% of the lower leg length, and stepping frequency at 15 cycles per min. $^1H$ NMR spectral analysis was performed in urine collected from all participants before and after eccentric exercise-induced muscle damage conventionally determined using a visual analogue scale (VAS) and maximal voluntary contraction (MVC). Urinary metabolic profiles were built by multivariate analysis of principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA) using SIMCA-P. From the OPLS-DA, men and women were separated 2 hr after the eccentric exercise and the separated patterns were maintained or clarified until 96 hr after the eccentric exercise. Subsequently, urinary metabolic profiles showed distinct trajectory patterns between men and women. Finally, we found increased urinary metabolites (men: alanine, asparagine, citrate, creatine phosphate, ethanol, formate, glucose, glycine, histidine, and lactate; women: adenine) after the eccentric exercise. These results could contribute to understanding metabolic responses following eccentric exercise-induced muscle damage in humans.