DOI QR코드

DOI QR Code

Study of Metabolic Profiling Changes in Colorectal Cancer Tissues Using 1D 1H HR-MAS NMR Spectroscopy

  • Kim, Siwon (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Sangmi (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Maeng, Young Hee (School of Medicine, Jeju National University) ;
  • Chang, Weon Young (School of Medicine, Jeju National University) ;
  • Hyun, Jin Won (School of Medicine, Jeju National University) ;
  • Kim, Suhkmann (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
  • Received : 2013.01.16
  • Accepted : 2013.02.20
  • Published : 2013.05.20

Abstract

Metabolomics is a field that studies systematic dynamics and secretion of metabolites from cells to understand biological pathways based on metabolite changes. The metabolic profiling of intact human colorectal tissues was performed using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy, which was unnecessary to extract metabolites from tissues. We used two different groups of samples, which were defined as normal and cancer, from 9 patients with colorectal cancer and investigated the samples in NMR experiments with a water suppression pulse sequence. We applied target profiling and multivariative statistical analysis to the analyzed 1D NMR spectra to identify the metabolites and discriminate between normal and cancer tissues. Cancer tissue showed higher levels of arginine, betaine, glutamate, lysine, taurine and lower levels of glutamine, hypoxanthine, isoleucine, lactate, methionine, pyruvate, tyrosine relative to normal tissue. In the OPLS-DA (orthogonal partial least square discriminant analysis), the score plot showed good separation between the normal and cancer groups. These results suggest that metabolic profiling of colorectal cancer could provide new biomarkers.

Keywords

References

  1. Ma, Y. L.; Qin, H. L.; Liu, W. J.; Peng, J. Y.; Huang, L.; Zhao, X. P.; Cheng, Y. Y. Digestive Diseases and Sciences 2009, 54, 2655- 2662. https://doi.org/10.1007/s10620-008-0665-4
  2. Chan, E. C.; Koh, P. K.; Mal, M.; Cheah, P. Y.; Eu, K. W.; Backshall, A.; Cavill, R.; Nicholson, J. K.; Keun, H. C. Journal of Proteome Research 2009, 8, 352-361. https://doi.org/10.1021/pr8006232
  3. Nicholson, J. K.; Connelly, J.; Lindon, J. C.; Holmes, E. Nature Reviews Drug Discovery 2002, 1, 153-161. https://doi.org/10.1038/nrd728
  4. Nicholson, J. K.; Lindon, J. C. Nature 2008, 455, 1054. https://doi.org/10.1038/4551054a
  5. Nicholson, J. K.; Lindon, J. C.; Holmes, E. Xenobiotica 1999, 29, 1181-1189. https://doi.org/10.1080/004982599238047
  6. Bezabeh, T.; Somorjai, R. L.; Smith, I. C. Magnetic Resonance in Chemistry 2009, 47, S54-S61. https://doi.org/10.1002/mrc.2530
  7. Cheng, L. L.; Lean, C. L.; Bogdanova, A.; Wright, S. C., Jr.; Ackerman, J. L.; Brady, T. J.; Garrido, L. Magnetic Resonance in Medicine 1996, 36, 653-658. https://doi.org/10.1002/mrm.1910360502
  8. Rocha, C. M.; Carrola, J.; Barros, A. S.; Gil, A. M.; Goodfellow, B. J.; Carreira, I. M.; Bernardo, J.; Gomes, A.; Sousa, V.; Carvalho, L.; Duarte, I. F. Journal of Proteome Research 2011, 10, 4314- 4324. https://doi.org/10.1021/pr200550p
  9. Holmes, E.; Loo, R. L.; Stamler, J.; Bictash, M.; Yap, I. K.; Chan, Q.; Ebbels, T.; De Iorio, M.; Brown, I. J.; Veselkov, K. A.; Daviglus, M. L.; Kesteloot, H.; Ueshima, H.; Zhao, L.; Nicholson, J. K.; Elliott, P. Nature 2008, 453, 396-401. https://doi.org/10.1038/nature06882
  10. Carrola, J.; Rocha, C. M.; Barros, A. S.; Gil, A. M.; Goodfellow, B. J.; Carreira, I. M.; Bernardo, J.; Gomes, A.; Sousa, V.; Carvalho, L.; Duarte, I. F. Journal of Proteome Research 2011, 10, 221-230. https://doi.org/10.1021/pr100899x
  11. Zhang, S.; Nagana Gowda, G. A.; Asiago, V.; Shanaiah, N.; Barbas, C.; Raftery, D. Analytical Biochemistry 2008, 383, 76-84. https://doi.org/10.1016/j.ab.2008.07.041
  12. OuYang, D.; Xu, J.; Huang, H.; Chen, Z. Applied Biochemistry and Biotechnology 2011, 165, 148-154. https://doi.org/10.1007/s12010-011-9240-0
  13. Maclntyre, D. A.; Jiménez, B.; Lewintre, E. J.; Martin, C. R.; Schäfer, H.; Ballesteros, C. G.; Mayans, J. R.; Spraul, M.; García- Conde, J.; Pineda-Lucena, A. Leukemia 2010, 24, 788-797. https://doi.org/10.1038/leu.2009.295
  14. Tiziani, S.; Lopes, V.; Günther, U. L. Neoplasia 2009, 11, 269- 276.
  15. Blasco, H.; Corcia, P.; Moreau, C.; Veau, S.; Fournier, C.; Vourc'h, P.; Emond, P.; Pradat, P. F.; Praline, J.; Devos, D.; Nadal-Desbarats, L.; Andres, C. R. PLoS One 2010, 5, e13223. https://doi.org/10.1371/journal.pone.0013223
  16. Rosenling, T.; Stoop, M. P.; Smolinska, A.; Muilwijk, B.; Coulier, L.; Shi, S.; Dane, A.; Christin, C.; Suits, F.; Horvatovich, P. L.; Wijimenga, S. S.; Buydens, L. M.; Vreeken, R.; Hankemeier, T.; van Gool, A. J.; Luider, T. M.; Bischoff, R. Clinical Chemistry 2011, 57, 1703-1711. https://doi.org/10.1373/clinchem.2011.167601
  17. Beckonert, O.; Coen, M.; Keun, H. C.; Wang, Y.; Ebbels, T. M.; Holmes, E.; Lindon, J. C.; Nicholson, J. K. Nature Protocols 2010, 5, 1019-1032. https://doi.org/10.1038/nprot.2010.45
  18. Moka, D.; Vorreuther, R.; Schicha, H.; Spraul, M.; Humpfer, E.; Lipinski, M.; Foxall, P. J. D.; Nicholson, J. K.; Lindon, J. K. Analytical Communications 1997, 34, 107-109. https://doi.org/10.1039/a701456b
  19. Rooney, O. M.; Troke, J.; Nicholoson, J. K.; Griffin, J. L. Magnetic Resonance in Medicine 2003, 50, 925-930. https://doi.org/10.1002/mrm.10620
  20. Gao, H.; Lu, Q.; Liu, X.; Cong, H.; Zhao, L.; Wang, H.; Lin, D. Cancer Science 2009, 100, 782-785. https://doi.org/10.1111/j.1349-7006.2009.01086.x
  21. Hong, Y. S.; Coen, M.; Rhode, C. M.; Reily, M. D.; Robertson, D. G.; Holmes, E.; Lindon, J. C.; Nicholson, J. K. Magnetic Resonance in Chemistry 2009, 47, S47-S53. https://doi.org/10.1002/mrc.2521
  22. Bollard, M. E.; Garrod, S.; Holmes, E.; Lindon, J. C.; Humpfer, E.; Spraul, M.; Nicholson, J. K. Magnetic Resonance in Medicine 2000, 44, 201-207. https://doi.org/10.1002/1522-2594(200008)44:2<201::AID-MRM6>3.0.CO;2-5
  23. Backshall, A.; Alferez, D.; Teichert, F.; Wilson, I. D.; Wilkinson, R. W.; Goodlad, R. A.; Keun, H. C. Journal of Proteome Research 2009, 8, 1423-1430. https://doi.org/10.1021/pr800793w
  24. Cheng, L. L.; Chang, I. W.; Smith, B. L.; Gonzalez, R. G. Journal of Magnetic Resonance 1998, 135, 194-202. https://doi.org/10.1006/jmre.1998.1578
  25. Cheng, L. L.; Chang, I. W.; Louis, D. N.; Gonzalez, R. G. Cancer Research 1998, 58, 1825-1832.
  26. Griffin, J. L.; Troke, J.; Walker, L. A.; Shore, R. F.; Lindon, J. C.; Nicholson, J. K. FEBS Letters 2000, 486, 225-229. https://doi.org/10.1016/S0014-5793(00)02307-3
  27. Kim, S.; Kim, S.; Lee, S.; Kwon, B.; Choi, J.; Hyun, J. W.; Kim, S. Bulletin of the Korean Chemical Society 2011, 32, 2021-2026. https://doi.org/10.5012/bkcs.2011.32.6.2021
  28. Moreno, B.; Rodrigues, C. O.; Bailey, B. N.; Urbina, J. A.; Moreno, S. N.; Docampo, R.; Oldfield, E. FEBS Letters 2002, 523, 207- 212. https://doi.org/10.1016/S0014-5793(02)02977-0
  29. Shulaev, V. Briefings in Bioinformatics 2006, 7, 128-139. https://doi.org/10.1093/bib/bbl012
  30. Wishart, D. S. Briefings in Bioinformatics 2007, 8, 279-293. https://doi.org/10.1093/bib/bbm030
  31. Wishart, D. S. Trends in Analytical Chemistry 2008, 27, 228-237. https://doi.org/10.1016/j.trac.2007.12.001
  32. Wold, S.; Esbensen, K.; Geladi, P. Chemometrics and Intelligent Laboratory Systems 1987, 2, 37-52. https://doi.org/10.1016/0169-7439(87)80084-9
  33. Wold, S. Pattern Recognition 1976, 8, 127-139. https://doi.org/10.1016/0031-3203(76)90014-5
  34. Trygg, J.; Wold, S. Journal of Chemometrics 2002, 16, 119-128. https://doi.org/10.1002/cem.695
  35. Bylesjo, M.; Rantalainen, M.; Cloarec, O.; Nicholson, J. K.; Holmes, E.; Trygg, J. Journal of Chemometrics 2006, 20, 341-351. https://doi.org/10.1002/cem.1006
  36. Waters, N. J.; Garrod, S.; Farrant, R. D.; Haselden, J. N.; Connor, S. C.; Connelly, J.; Lindon, J. C.; Holmes, E.; Nicholson, J. K. Analytical Biochemistry 2000, 282, 16-23. https://doi.org/10.1006/abio.2000.4574
  37. Tikunov, A. P.; Johnson, C. B.; Lee, H.; Stoskopf, M. K.; Macdonald, J. M. Marine Drugs 2010, 8, 2578-2596. https://doi.org/10.3390/md8102578
  38. Aruoma, O. I.; Halliwell, B.; Hoey, B. M.; Butler, J. Biochemical Journal 1988, 256, 251-255.
  39. Schaffer, S.; Takahashi, K.; Azuma, J. Amino Acids 2000, 19, 527- 546. https://doi.org/10.1007/s007260070004
  40. Birdsall, T. C. Alternative Medicine Review 1998, 3, 128-136.
  41. Kirk, K.; Kirk, J. FEBS Letters 1993, 336, 153-158. https://doi.org/10.1016/0014-5793(93)81630-I
  42. Downes, C. P.; Macphee, C. H. European Journal of Biochemistry 1990, 193, 1-18. https://doi.org/10.1111/j.1432-1033.1990.tb19297.x
  43. Krajcovicova-Kudlackova, M.; Simoncic, R.; Bederova, A.; Babinska, K.; Beder, I. Physiological Research 2000, 49, 399- 402.
  44. Halliwell, B.; Gutteridge, J. Free Radicals in Biology and Medicine 1999, 3rd ed. (Oxford Univ. Press, Oxford).
  45. Meister, A. Science 1983, 220, 472-477. https://doi.org/10.1126/science.6836290
  46. Martindale, J. L.; Holbrook, N. J. Journal of Cellular Physiology 2002, 192, 1-15. https://doi.org/10.1002/jcp.10119
  47. Monleon, D.; Morales, J. M.; Barrasa, A.; Lopez, J. A.; Vazquez, C.; Celda, B. NMR in Biomedicine 2009, 22, 342-348. https://doi.org/10.1002/nbm.1345
  48. Nakagami, K.; Uchida, T.; Ohwada, S.; Koibuchi, Y.; Suda, Y.; Sekine, T.; Morishida, Y. Cancer Science 1999, 90, 419-424. https://doi.org/10.1111/j.1349-7006.1999.tb00764.x
  49. Lin, E. C. Annual Review of Biochemistry 1977, 46, 765-795. https://doi.org/10.1146/annurev.bi.46.070177.004001
  50. Waterhouse, C. Cancer 1974, 33, 66-71. https://doi.org/10.1002/1097-0142(197401)33:1<66::AID-CNCR2820330113>3.0.CO;2-0

Cited by

  1. Applications of NMR spectroscopy based metabolomics: a review vol.17, pp.1, 2013, https://doi.org/10.6564/JKMRS.2013.17.1.001
  2. Metabonomics of Human Colorectal Cancer: New Approaches for Early Diagnosis and Biomarker Discovery vol.13, pp.9, 2014, https://doi.org/10.1021/pr500443c
  3. Meeting report: The biology of genomes and proteomes vol.37, pp.7, 2015, https://doi.org/10.1007/s13258-015-0305-2
  4. Ameliorative effects of pyrazinoic acid against oxidative and metabolic stress manifested in rats with dimethylhydrazine induced colonic carcinoma vol.18, pp.5, 2017, https://doi.org/10.1080/15384047.2017.1310341