• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.408-413
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• 2007

Putrescine has a negative effect on health and is also used as an indicator of quality on meat products. We investigated the genes involved in putrescine production by Serratia liquefaciens IFI65 isolated from a spoiled Spanish dry-cured ham. We report here the genetic organization of its ornithine decarboxylase encoding region. The 5,506-bp DNA region showed the presence of three complete and two partial open reading frames. Putative functions have been assigned to several gene products by sequence comparison with proteins included in the databases. The second gene putatively coded for an ornithine decarboxylase. The functionality of this decarboxylase has been experimentally demonstrated by complementation to an E. coli defective mutant. Based on sequence comparisons of some enterobacterial ornithine decarboxylase regions, we have elaborated a hypothetical pathway for the acquisition of putrescine biosynthetic genes in some Enterobacteriaceae strains.

• 한국식품영양과학회지
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• 제24권3호
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• pp.415-419
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• 1995

Ornithine decarboxylase(ODC) catalyzes the first and key step in the polyamine biosynthetic pathway. Ornithine decarboxylase is known to the enzyme that increase substantially its activity in regenerating liver. We found that activity and mRNA level for ODC increase significantly after partial hepatectomy in the rat. After laparotomy, there was significant decrease in activity ; however, mRNA content was unaltered in contrast to previous reports of no change in ornithine decarboxylase and thymidine kinase after sham hepatectomy. This may be mediated by the decrease in food intake after hepatectomy. Therefore it is necessary to examine the effect of food intake after hepatectomy on the ODC activity and mRNA level in the future.

• Journal of Plant Biology
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• 제27권1호
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• pp.1-6
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• 1984

Kinetin(4.7$\times$10\ulcornerM) and 6-benzylaminopurine (2.2$\times$10\ulcornerM) were found to increase ca. 1.5-fold putrescice content in corn grown in medium containing kinetin and 6-benzylaminopurine(6-BAP) for 3days whereas kinetin was found to decrase ca. 30% spermidine and spermine, respectively. KCI (3$\times$10\ulcornerM) was found to decrease more than 50% putrescine content. After germination, ornithine decarboxylase activity was observed to increase constantly whereas arginine decarboxylase activity remained constant, suggesting involvement in putrescine biosynthesis. 6-benzylaminopurine was shown to increase more activities of arginine ornithine decarboxylase than kinetin when they were added to medium.

• 생약학회지
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• 제35권4호통권139호
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• pp.283-287
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• 2004

The effect of water extract from Cnidii Rhizoma (CRW) on proliferation of human breast cancer cells, nitric oxide production, nitric oxide synthase expression, and ornithine decarboxylase activity was tested. CRW inhibited the growth of both estrogen-dependent MCF-7 and estrogen-independent MDA-MB-23I human breast cancer cells. Lipopolysaccharide-induced nitric oxide (NO) production was significantly reduced by CRW at the concentration of 0.5, 1.0 and 5.0 mg/ml. Expression of inducible nitric oxide synthase (iNOS) was also suppressed with the treatment of CRW in Raw 264.7 cells. CRW inhibited induction of ornithine decarboxylase by 12-0-tetradecanoylphorbol-13-acetate, a key enzyme of polyamine biosynthesis, which is enhanced in tumour promotion. Therefore, CRW is worth further investigation with respect to breast cancer chemoprevention or therapy.

• 한국환경보건학회지
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• 제21권1호
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• pp.78-85
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• 1995

Ornithine decarboxylase(ODC) is an indicate enzyme in carcinogenesis. We divided Sprague Dawley rats into six groups: control, electric field exposure, X-ray only irradiation, X-ray irradiation with electric field exposure group, Sr-90 injected group and Sr-90 and electric field complex exposure group. The ODC activity was measured in rat's bone marrow cell every week. The results were summarized as follows: The ODC activitied was increased in X-ray irradiated, Sr-90 injected and Sr-90 and electric field complex exposed group as compared with that of control(p<0.05). The ODC activity was increased comparing that of control neither in X-ray and electric field complex exposed group nor electric field only exposed group. This result suggests that the electric field doesn't have myeloid carcinogenicity and myeloid cancer incidence caused by ionized radiation is suppressed by electric field exposure.

• BMB Reports
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• 제34권5호
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• pp.472-477
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• 2001

Ornithine decarboxylase (ODC, EC 4.1.1.17) is the first and key enzyme in eukaryotic polyamine biosynthesis. The cDNA encoding ornithine decarboxylase from Nicotiana glutinosa was cloned ($GeBank^{TM}$ AF 323910) and expressed in E. coli. Site directed mutagenesis were performed on several highly conserved cysteine residues. Among the mutants, C115A showed significant changes in the kinetic properties. The $K_m$ value of the C115A mutant was $1790\;{\mu}M$, which was 3-fold higher than that of the wild-type ODC. There was a dramatic decrease in the $k_{cat}$, values of the C115A mutant, compared to that of the wild-type ODC, which had a $k_{cat}$ value of $77.75\;s^{-1}$. C115A caused a shift in the optimal pH from 8.0 to 8.4. Considering these results, we suggest that cys-115 is involved in the catalytic activity of N. glutinosa ODC.

• Journal of Plant Biology
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• 제39권2호
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• pp.107-112
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• 1996

To study the compensatory aspect of putrescine biosynthetic enzyme n tobacco suspension cultured cells, we examined the contents of the cellular polyamines and the activities of arginine decarboxylase (ADC, EC 4.1.1.19) and ornithine decarboxylase (ODC, EC 4.1.1.17) in the tobacco suspension cells treated with $\alpha$-difluoromethyl arginine (DFMA) or $\alpha$-difluoromethyl ornithine (DFMO). In the untreated cells, the content of the cellular putrescine was decreased during the first 3 hours and then subsequently increased. However, the content of the cellular spermidine and spermine remained constant during the incubation time. While ADC activity increased after 6 hours, ODC activity decreased following the rapid increase until 6 hours. DFMA induced the decrease in the contents of putrescine and spermidine, and the increase in that of spermine. It also caused the inhibition of ADC and ODC activities throughout the incubation time. DFMO produced the stimulation of ADC activity about 2 times of untreated cells and the decrease in the content of putrescine about 50% of them at 12 hour. The application of putrescine or cycloheximide prevented the increase of ADC activity by DFMO but that of actinomycin-D did not show any detectable effect. The stimulation of ADC activity by DFMO in tobacco suspension cultured cells was probably due to the enhancement of de novo synthesis for ADC protein, which might be regulated in the translation step by the content of the cellular putrescine.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2425-2427
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• 2012

Ornithine decarboxylase (ODC), the first enzyme in the polyamine biosynthesis, plays an important role in tumor progression, cell proliferation and differentiation. In recent years, ODC has been the subject of intense study among researchers, as a target for anti-cancer therapy and specific inhibitory agents, have the potential to suppress carcinogenesis and find applications in clinical therapy. In particular, it is suggested that ODC is a promising candidate target for natural products in cancer chemoprevention. Future exploration of ornithine decarboxyalse inhitors present in nature may offer great hope for finding new cancer chemporeventive agents.

• 동의생리병리학회지
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• 제20권6호
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• pp.1572-1575
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• 2006

Water extract from Cnidii Rhizoma (CRW) was tested for colon cancer chemopreventive activity by measuring the induction of phase II detoxification enzyme activity [quinone reductase (QR) and glutathione S-transferase (GST)] and glutathion (GSH) levels and ornithine decarboxylase (ODC) activity in cultured human colorectal adenocarcinoma HT-29 cells. CRW inhibited cell proliferation in cultured HT-29 cells. CRW induced QR activity in a dose-dependent manner in a concentration range of 0.1${\sim}$5.0 $mg/m{\ell}$. GST activity was also induced with the treatment of CRW in HT-29 cells. In addition GSH levels was increased with CRW. CRW inhibited ODC activity, a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. These results suggest that CRW has colon cancer chemopreventive activity by increasing phase II enzyme activity and GSH levels and inhibiting ODC activity in vitro.

• 한국해양바이오학회지
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• 제1권2호
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• pp.71-75
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• 2006

별불가사리 단백추출물을 조제하여 유방암 유발억제효과를 측정한 결과, 별불가사리 단백추출물은 estrogen-의존성의 유방암세포(MCF-7)와 estrogen-비의존성 유방암세포(MDA-MB-231)의 증식을 농도의존적으로 억제하는 효과가 있었으며, 유방암발생 발암물질 활성에 관여하는 cytochrome P450 1A1 활성도 저해하였다. 또한 유방암발생의 촉진단계에 주요한 기능을 가진 ODC 활성도 억제하였으므로 별불가사리 단백추출물은 유방암 발생을 저해할 것으로 기대된다.