Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Gene Cloning, Expression, and Functional Characterization of an Ornithine Decarboxylase Protein from Serratia liquefaciens IFI65

  • De Las Rivas Blanca (Departamento de Microbiologia, Instituto de Fermentaciones Industriales, CSIC) ;
  • Carrascosa Alfonso V. (Departamento de Microbiologia, Instituto de Fermentaciones Industriales, CSIC) ;
  • Munoz Rosario (Departamento de Microbiologia, Instituto de Fermentaciones Industriales, CSIC)
  • Published : 2007.03.31
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Abstract

Putrescine has a negative effect on health and is also used as an indicator of quality on meat products. We investigated the genes involved in putrescine production by Serratia liquefaciens IFI65 isolated from a spoiled Spanish dry-cured ham. We report here the genetic organization of its ornithine decarboxylase encoding region. The 5,506-bp DNA region showed the presence of three complete and two partial open reading frames. Putative functions have been assigned to several gene products by sequence comparison with proteins included in the databases. The second gene putatively coded for an ornithine decarboxylase. The functionality of this decarboxylase has been experimentally demonstrated by complementation to an E. coli defective mutant. Based on sequence comparisons of some enterobacterial ornithine decarboxylase regions, we have elaborated a hypothetical pathway for the acquisition of putrescine biosynthetic genes in some Enterobacteriaceae strains.

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References

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