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Effect of Cnidii Rhizoma on Phase II Enzyme and Ornithine Decarboxylase Activities  

Shon, Yun-Hee (Intractable Disease Research Center, Dongguk University)
Kim, Mee-Kyung (Intractable Disease Research Center, Dongguk University)
Cho, Hyun-Jung (Intractable Disease Research Center, Dongguk University)
Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.6, 2006 , pp. 1572-1575 More about this Journal
Abstract
Water extract from Cnidii Rhizoma (CRW) was tested for colon cancer chemopreventive activity by measuring the induction of phase II detoxification enzyme activity [quinone reductase (QR) and glutathione S-transferase (GST)] and glutathion (GSH) levels and ornithine decarboxylase (ODC) activity in cultured human colorectal adenocarcinoma HT-29 cells. CRW inhibited cell proliferation in cultured HT-29 cells. CRW induced QR activity in a dose-dependent manner in a concentration range of 0.1${\sim}$5.0 $mg/m{\ell}$. GST activity was also induced with the treatment of CRW in HT-29 cells. In addition GSH levels was increased with CRW. CRW inhibited ODC activity, a key enzyme of polyamine biosynthesis, which is enhanced in tumor promotion. These results suggest that CRW has colon cancer chemopreventive activity by increasing phase II enzyme activity and GSH levels and inhibiting ODC activity in vitro.
Keywords
Cnidii Rhizoma; quinone reductase; glutathione S-transferase; glutathione; ornithine decarboxylase;
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