• 한국미생물·생명공학회지
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• 제16권2호
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• pp.111-118
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• 1988

유기산 생산균인 L. casei Y, Str. faecium C, Str. faecalis, Cl. butyricum M, B. toyoi를 사료첨가제와 발효유로부터 분리 확인하여 이들 균주가 자돈의 대장균증 설사를 유발시키는 E. coli $A_2$, E. coli G$_7$에 대하여 in vitro에서는 어떤 항균효과를 나타내는지 조사한 결과를 요약하면 다음과 같다. 1. L. casei Y, Str. faecium C, Str. faecalis, B. toyoi Cl. butyricum M을 각각 배양한 BL broth 3일 배양액은 모두 E. coli $A_2$와 E. coli G$_7$에 대하여 항균효과를 나타내었고, 이들 배양액을 E. coli $A_2$와 E. coli G$_7$에 대한 발육 Disc 확산시험 결과 그 저지환 직경은 각각 15mm와 17mm, 14mm와 15mm, 13mm와 14mm, 11mm와 13 mm, 10mm와 12mm 였다. 2. 유기산 생산 능력이 우수한 균일수록 E. coli $A_2$와 E. coli G$_7$에 대한 항균력이 높게 관찰되었으며, L. casei Y와 Str. faecium C가 이들 두 대장균에 대하여 높은 항균력을 나타내었다. 3. 유기산 생성균과 대장균(E. coli $A_2$, E. coli G$_7$)을 BL broth 에 혼합 배양할 때 E. coli $A_2$와 E. coli G$_7$은 배양액의 pH가 5.0 이하일 때 생육억제 되었고, pH 4.5 이하였을 때 사멸되었다. 4. 생성된 유기산은 배양액의 pH를 저하시킴과 동시에 항균성 물질로 직접 작용하여 E. coli $A_2$와 E. coli G$_7$의 생육저해 또는 사멸을 유도하였다. 5. E. coli $A_2$가 E. coli G$_7$보다 유기산 생산균의 대사산물에 대하여 더 높은 내성을 갖고 있었다.

• 한국식품영양학회지
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• 제3권2호
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• pp.141-148
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• 1990

This studies were carried out to investigate the effects of non-volatile organic acids in the KimChi by lactic acid bacteria. The organism isolated from KimChi, Pediococcus dextrinicus, Leuconostoc mesenteroides, Lactobacillus plantarum and Lactobacillus brevis, were inoculated for preparation of KimChi. pH of all on the KimChi sample dropped sharply according as fermentation continued. pH of on optimum ripening period KimChi(4.4 and 4.2) reached 1.3 and 1.9 day at all on sample, respectively. Optimum acidity(0.5%) of KimChi were reached within 2 day all on sample. The total number of lactic acid bacteria reached 1.0X107cells/ml in 1 day and decreased slowly after 4 day. Main non-volatile organic acids were identified lactic, malic and succinic acid. The sensory score of mixed lactic acid bacteria inoculated KimChi was better than that of another KimChi.

• Journal of Dairy Science and Biotechnology
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• 제40권1호
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• pp.23-34
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• 2022

In this study, to determine the importance of lactic acid bacteria (LAB) in Kombucha fermentation, biological functions, such as organic acid production and anti-inflammatory and antibacterial activities, of Kombucha, with or without LAB inoculation, were evaluated. Lactobacillus paracasei DK215, Saccharomyces cerevisiae C3, and Acetobacter pasteurianus P2 were selected as the inoculants. Organic acids were measured every 3 days from the end of fermentation using HPLC; the organic acid content of LAB-inoculated Kombucha was relatively high. Samples with or without LAB inoculation showed high antibacterial activity against Escherichia coli. The MTT assay results indicated no significant difference in concentration difference and cell death. In the NO production test, compared with the uninoculated Kombucha sample, the LAB-inoculated Kombucha sample exhibited a value similar to that of the group without LPS treatment. The levels of cytokine (IL-1α, IL-6, TNF-α) production were significantly lower than those of the LPS(+) group, indicating the anti-inflammatory activity potential of the Kombucha sample. This improvement in the biological function of the LAB-inoculated Kombucha further verifies the value of LAB in the fermented food and beverage industry.

• Ocean Science Journal
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• 제40권3호
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• pp.145-153
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• 2005

Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria - were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.

• 한국수소및신에너지학회논문집
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• 제13권4호
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• pp.321-329
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• 2002

Clostridium butyricum, Lactobacillus amylophillus, Lactobacillus amylovorus, Lactobacillus acidophillus, AI-9 produced hydrogen and /or organic acids using glucose, lactose and starch at the anaerobic culture conditions. Cl. butyricum NCIB 9576 evolved 1,700 ml H2/L-culture broth and accumulated butyric acid, acetic acid, propionic acid and ethanol in its culture broth when lactose was used as a carbon source during 24 hrs of fermentation. L. amylovorus ATCC 33620 accumulated lactic and acetic acids and some reducing sugars when starch was used as a carbon source without hydrogen production. Instead of starch as a carbon source, L. amylovorus ATCC 33620 produced lactic acid from algal biomass during fermentation and the acid-heat or freeze-thaw pretreatment of algal biomass accelerate the lactic acid fermentation.

• 한국토양비료학회지
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• 제49권6호
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• pp.783-788
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• 2016

Agricultural management of paddy soil depends on the effects of soil microbial activities. The present study evaluated the soil microbial community of 25 paddy soils in Gyeongnam Province by fatty acid methyl ester (FAME). The average of microbial communities in paddy soils were 32.2% of total bacteria, 16.7% of Gram-negative bacteria, 12.9% of Gram-positive bacteria, 2.0% of actinomycetes, 14.9% of fungi, and 1.3% of arbuscular mycorrhizal fungi. The communities of total bacteria (34.9%) and Gram-negative bacteria (19.4%) in soils with $30{\sim}35g\;kg^{-1}$ of organic matter were significantly larger than those in soils with other organic matter levels. However, soils with $20{\sim}30g\;kg^{-1}$ of organic matter had significantly low ratio of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ as compared with soils with $30{\sim}35g\;kg^{-1}$ of organic matter, indicating microbial stress decreased (p < 0.05). In principal component analyses of soil microbial communities, Gram-negative bacteria should be considered as a potential responsible factor for the obvious microbial community differentiation that was observed between the two different organic matter levels in paddy fields. Thus, soils containing $20{\sim}30g\;kg^{-1}$ of organic matter were responsible for strong effect on microbial biomass and stress in paddy fields.

• 한국초지조사료학회지
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• 제39권3호
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• pp.132-140
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• 2019

Fermented total mixed ration (TMR) is a novel feed for ruminants in South Korea. The purpose of this study was to evaluate the effects of lactic acid bacteria (LAB) on the quality of TMR and in vitro ruminal fermentation. Strains of three LAB spp. (Lactobacillus plantarum, L. brevis, L. mucosae) were used in fermentation of TMR. Inoculations with the three LAB spp. lowered pH and increased concentrations of lactic acid, acetic acid, and total organic acid compared to non-LAB inoculated control (only addition of an equivalent amount of water) (p<0.05). Bacterial composition indicated that aerobic bacteria and LAB were higher. However, E. coli were lower in the fermented TMR than those in the control treatment (p<0.05). Among the treatments, L. brevis treatment had the highest concentration of total organic acid without fungus detection. Gas production, pH, and ammonia-nitrogen during ruminal in vitro incubation did not differ throughout incubation. However, ruminal total VFA concentration was higher (p<0.05) in the LAB spp. treatments than the control treatment at 48 hours. Overall, the use of L. brevis as an inoculant for fermentation of high moisture. TMR could inhibit fungi growth and promote lactic fermentation, and enhance digestion in the rumen.

• 한국유기농업학회지
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• 제25권2호
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• pp.461-473
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• 2017

Total mixed ration (TMR) including concentrate diet and roughage together have been used for the ruminant animal. Relatively high concentrations of moisture and water soluble carbohydrate are representative feature of TMR. Those moisture and water can also provide a niche for bacterial growth. Therefore, a possible fermentation of TMR induced by micro-organism is generally accepted. The present study hypothesized that different lactic acid bacteria could alter fermentation of TMR and subsequently rumen fermentation. Three lactic acid bacteria, Lactobacillus paracasei (A), L. plantarum (B) and L. parabuchneri (C), were employed and 7 treatments under full factorial design were compared with control without inoculation. TMR for dairy cow was used. Significant alterations by treatments were detected at lactic acid and butyric acid contents in TMR (p<0.05). Treatment AC (mixture of A and C) and BC (mixture of B and C) showed great lactate production. Great butyrate production was found at treatment C. At in vitro rumen fermentation, treatments B, C and AB (mixture of A and B) showed significantly great total gas production (p<0.05). All treatments except treatments B and AB, showed less dry matter digestibility, significantly (p<0.05). Total volatile fatty acid production at treatment AC was significantly greater than others (p<0.05). In individual volatile fatty acid production, treatment AB and AC showed great acetate and propionate productions, significantly (p<0.05). This study investigated correlation between organic acid production in TMR and rumen volatile fatty acid production. And it was found that butyric acid in TMR had significant negative correlation with acetate, propionate, total volatile fatty acid, AP ratio and dry matter digestibility.

• Food Science and Biotechnology
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• 제16권6호
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• pp.958-962
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• 2007

This study was conducted to determine the effects of microorganism inactivation using 3 ppm of aqueous ozone (AO), 1% citric acid, 1% lactic acid, and 1% acetic acid alone, as well as the combinations of AO and organic acid, for washing the raw materials of saengsik (carrot, cabbage, glutinous rice, barley) with or without agitation. The combination of AO and 1% of each organic acid significantly inactivated spoilage bacteria in both the vegetables and the grains (p<0.05). However, in the glutinous rice, no inhibitory effects were shown for total aerobic bacteria by using water, ozone, or the combination of AO with citric acid or lactic acid, without agitation. Microbial inactivation was enhanced with agitation in the grains, whereas dipping (no agitation) treatments showed better inhibitory effects in the vegetables than in the barley, suggesting that washing processes should take into account the type of food material.

• 생명과학회지
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• 제22권10호
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• pp.1324-1329
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• 2012

본 연구의 목적은 organic acids를 첨가하여 in vitro 상의 반추위 발효성상과 반추위 내 메탄 억제에 미치는 영향에 대한 효과를 알아보고자 하였다. 반추위액은 순천대학교 부속목장의 반추위 cannula가 시술된 Holstein에서 채취하였고, organic acids는 반추위액과 버퍼의 혼합액에 첨가하여 배양하였다. 그 결과 pH 값은 lactic acid, malic acid 및 succinic acid첨가구에서 6.69에서 6.16 정도로, 대조구와 다른 첨가구보다 낮았다. 총 가스 발생량은 배양 48시간에 aspartic acid, malic acid 및 succinic acid첨가구에서 유의적(p<0.05)으로 높았고, 메탄 발생량은 lactic acid 첨가구에서 대조구보다 낮았다. 총 VFA와 propionic acid의 농도는 배양 12시간에 모든 첨가구가 대조구에 비해 높았다. 반추위 미생물 측정 결과에서는 Fumaric acid와 malic acid의 bacteria수가 대조구에 비해 유의적으로 증가하였으며(p<0.05), protozoa수는 유의적(p<0.05)으로 감소되었다. 이상의 실험 결과를 종합해 보면, organic acids의 첨가는 반추위 내 pH를 감소시키고 가스 발생량, 반추위 미생물 성장량 및 propionic acid 모두 증가시켰으며, 특히 lactic acid는 메탄생성을 억제하였다. 앞으로 Organic acid와 다른 메탄억제 물질과 혼합하여 반추위 내 메탄생성 억제에 관한 구체적인 연구가 필요한 것으로 사료된다.