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http://dx.doi.org/10.5352/JLS.2012.22.10.1324

Effects of Organic Acids on In Vitro Ruminal Fermentation Characteristics and Methane Emission  

Ok, Ji Un (Animal Nutrition Team, National Institute of Animal Science, RDA)
Ha, Dong Uk (Division of Applied Life Science (BK21 program) & Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Lee, Shin Ja (Department of Animal Science, Gyeongbuk Provincial Collage)
Kim, Eun Tae (Division of Applied Life Science (BK21 program) & Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Lee, Sang Suk (Department of Animal Science & Biotechnology, Suncheon National University)
Oh, Young Kyun (Animal Nutrition Team, National Institute of Animal Science, RDA)
Kim, Kyoung Hoon (Animal Nutrition Team, National Institute of Animal Science, RDA)
Lee, Sung Sill (Division of Applied Life Science (BK21 program) & Institute of Agriculture and Life Science (IALS), Gyeongsang National University)
Publication Information
Journal of Life Science / v.22, no.10, 2012 , pp. 1324-1329 More about this Journal
Abstract
The objective of this study was to evaluate the in vitro effects of organic acids on methane emission and ruminal fermentation characteristics. We expected our methodology to result in a decrease of methanogens attached to the surface of rumen ciliate protozoa by addition of organic acids and in particular a decrease in methane emission. A fistulated Holstein cow of 650 kg body weight was used as a donor of rumen fluid. Organic acids (aspartic acid, fumaric acid, lactic acid, malic acid, and succinic acid) known to be propionate enhancers were added to an in vitro fermentation system and incubated with rumen fluid. The microbial population, including bacteria, protozoa, and fungi, were enumerated, and gas production, including methane and fermentation characteristics, were observed in vitro. Organic acids appeared to affect the rumen protozoan community. The rumen protozoal popuation decreased with the addition of aspartic acid, fumaric acid, lactic acid, and malic acid. In particular, the methane emission was reduced by addition of lactic acid. The concentration of propionate with all organic acids that were added appeared to be higher than that of the control at 12 h incubation. Addition of organic acids significantly affected rumen bacteria and microbial growth. The bacteria in added fumaric acid and malic acid was significantly higher (p<0.05) and protozoa was significantly lower (p<0.05) than that of the control. Microbial growth with the addition of organic acids was greater than the control after 48 h incubation.
Keywords
Organic acids; methane emission; ruminal fermentation; protozoa; VFA;
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