• 생명과학회지
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• 제14권1호
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• pp.126-130
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• 2004

곰보버섯(Morchella esculenta)은 맛과 영양이 풍부한 버섯으로 전 세계적으로 분포하는 버섯이다. 현재까지 우리나라에서 곰보버섯에 관한 체계적인 연구가 이루어지지 않은 상태에서 본 연구는 곰보버섯(자실체) 인공대량 생산방법 개발 및 심부배양을 통한 균사체 및 다당체 대량생산 공정개발을 위한 기초자료를 얻고자 shaking flask culture를 이용한 곰보버섯 균사체 배양특성에 관하여 조사하였다. 곰보버섯 균사체의 최적 배양온도는 $25^{\circ}C$이며, 최적 초기 pH는 6.5, 최적배지는 BG 이었다. BG 배지를 기본배지로 하여 영양 요구성 실험을 한 결과 최적탄소원은 fructose이고 최적농도는 5.0% (w/v)이며 질소원 선발 및 최적농도에서는 peptone과 NH$_4$Cl를 혼합하여 사용하였을 때 최대 균사체생육을 보였고, 상기의 두 질소원을 1:1의 무게비로 혼합하여 4.0% (w/v) 농도로 첨가하였을 때 최대 균사생육을 보였다.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.263-268
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• 1995

A screening was performed to isolate exoploysaccharide-producing microorganisms, which synthesized specific exopolysaccharide for the substitutive of commercial polysaccharides, from natural sources. Soil bacterium, one of 378 mucoid isolates, was finally selected as potential producer of polysaccharides which made the culture broth very viscous and thus examined in detail for optimal medium composition. Isolated strain was identified as Xanthomonas sp. EPS- 1 from the results of morphological and biochemical characteristics. The composition of optimal medium for exopolysaccharide production was as follows: 50 g sucrose, 1.5 g peptone, 2 g KH$_{2}$PO$_{4}$, 2 g MgSO$_{4}$, -7H$_{2}$O, 3 g NaCl, 0.05 g CaCO$_{3}$, 0.07 g FeSO$_{4}$-7H$_{2}$O and 0.05 g MnSO$_{4}$-7H$_{2}$O in 1 liter of distilled water. From the experiments of temperature and pH dependence, the optimal conditions for exopolysaccharide biosynthesis seemed to be 30$\circ$C and 8.0, respectively. About 14.9 gram of maximum exopolysaccharide per liter was obtained at the initial pH 8.0, 30$\circ$C and 250 rpm in a flask culture. The exopolysaccharide EPS-1 had such potential as an emulsifying agent and a gelling agent in comparision with commercial exopolysaccharide.

• Mycobiology
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• 제36권3호
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• pp.178-182
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• 2008

In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; $K_2HPO_4$, 0.46 g/l; $K_2HPO_4$, 1.00 g/l; M$MgSO_4\cdot7H_2SO$, 0.50 g/l; $FeCl_2\cdot6_2O$, 0.01 g/l; $MnCl_2\cdot4H_2O$, 0.036 g/l; $ZnCl_2$, 0.03 g/l; and $SuSO_4\cdot7H_2O$, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28$^{\circ}C$; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

• 생명과학회지
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• 제13권4호
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• pp.492-497
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• 2003

액체배양을 이용한 잣버섯(Lentinus lepideus KACC 50120)의 균사체 생육에 관한 결과로는 최적온도는 $25^{\circ}C$이고, 최적 pH는 5.5이었다. 공시배지중에서 최대 균사 생육을 보인 최적배지는 YMG 배지로 조사되었다. YMG 배지를 기본배지로 하여 영양 요구성 실험을 한 결과 탄소원 선발 및 최적 농도에서는 최적 탄소원은 glucose이고 최적 농도는 4.0%(w/v)이며 질소원 선발 및 최적 농도에서는 malt extract와 yeast extract를 혼합하여 사용하였을 때 최대 균사 생육을 보였고, 상기의 두가지 복합질소원을 1:1의 무게비로 혼합하여 1.5%(w/v)농도로 첨가하였을 때 균사생육이 가장 양호하였다.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.482-487
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• 2000

Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica ws studied. Maltose, yeast extract, and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in a flask culture were pH 5.0, $25^{\circ}C$, and 150 rpm in a medium containing (as in g/l) 30 maltose, 6 yeast extruct, 2 polypeptone, $0.5{\;}K_3HPO_4,{\;}0.2{\;}KH_2PO_4,{\;}0.2{\;}MnSO_4{\cdot}5H_2O,{\;}0.2{\;}MgSO_4{\cdot}7H_2O$. Exo-biopolymer production and mycelial growth in the above suggested medium were significantly increased in a 2.5-1 jar fermentor, where the maximum biopolymer concentration was 8 g/l. The morphological changes of the mycelium in the submerged culture were observed within pH ranges from 4.0 to 9.0; i.e., growth of the filamentous form was optimal at culture pHs of 5.0 and 6.0, whereas pellet was formed at other pHs.

• Journal of Biosystems Engineering
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• 제34권4호
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• pp.220-227
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• 2009

In the process of oyster mushroom production, loading work of culture medium needs the most intensive labor power. Therefore, development of culture medium machine causes to reduce the manpower and cost. The main objective of this study is to develop the culture medium loading machine and investigate the optimal operation conditions and to evaluate the economic value of the machine. The results are summarized as follows: 1. Optimum transporting velocity of the conveyor was 0.61 m/s 2. Optimum speed of blower was 3183 rpm at the transporting velocity of 0.61 m/s with the loading quantity of 3.41 t/hr 3. Recommendable opening area ratio of pressure controller was 1/2 at the blower speed of 3183 rpm and the transporting velocity of 0.61 m/s 4. The break even point resulted in $240\;m^2$ of cultivating area compared to the method of with portable workbench, and $350\;m^2$ of cultivating area compared to the method of with a tractor and a truck.

• 식물조직배양학회지
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• 제22권2호
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• pp.101-104
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• 1995

작약동아의 정아 및 액아의 기내배양에 의한 유묘의 증식에 필요한 배양조건을 구명코자 실험한 결과는 다음과 같다. 정아배양의 경우, 생장조절재의 조성에 무관하게 두지방종에서 100% 신초가 신장하였으나 생장은 의성지방종 은 NAA 0.1 mg/L 단용배지, 영천지방종은 NAA 0.01 mg/L 단용배지에서 가장 양호하였다. 액아배양의 경우, NAA 0.01 mg/L와 zeatin 5.0 mg/L 혼용배지에서 의성지방종은 100%, 영천지방종은 50%의 가장 높은 신초신장율을 보였고 신초의 생장도 양호한 경향이었다. 정아 및 액아배양으로부터 유도된 신초는 vermiculite를 지지물로 한 NAA 0.1 mg/L 첨가배지에서 30.0%의 발근율을 보였으나 뿌리의 생장은 양호하였다.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1115-1120
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• 2008

A novel cathepsin B inhibitor-producing bacterium was isolated from marine sediments and identified based on its 16S rDNA sequence as Pseudomonas sp. strain PB01 (Accession No. EU126129). The growth and enzyme inhibitor production were investigated under various culture conditions. A mixture of organic nitrogen source was required for the optimal production, whereas both glucose and maltose proved to be the effective carbon sources for cathepsin B inhibitor production. Other optimal culture conditions included temperature range between 25 and $28^{\circ}C$, initial medium pH of 6.6, and shaking speed of 200 rpm. Under these optimal conditions, the maximum inhibitory activity from culture broth was approximately 50% after 30 h of cultivation. Additionally, kinetic study revealed that inhibitor production paralleled with cell growth, which suggested that the inhibitor may be a primary metabolite of that bacterium.

• Journal of Plant Biology
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• 제35권1호
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• pp.1-8
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• 1992

Aphanothece속과 분류학적 특징이 유사한 남조식물 SG63을 대상으로 배양액의 NaCl과 $MgSO_4{\cdot}7H_2O$ 농도에 따른 최적 생리적 활성을 연구했다. SG63의 최고 성장률은 56%의 NaCl과 20%의 $MgSO_4{\cdot}7H_2O$ 농도의 배양액에서 각각 이루어졌다. 엽록소 a, phycocyanin과 수용성 단백질의 합성율은 두 무기염도에 따라 영향을 받았다. 특히, 엽록소 a와 phycocyanin의 농도는 NaCl 함량이 높은 배양액일수록 감소되었다. 남조식물 SG63의 carotenoid의 구성물질은 ${\beta}-carotene$, echinenone, zeaxanthin과 myxoxanthophyll이였다. 총단백질과 엽록소 a의 농도비율과 phycocyanin과 엽록소 a의 농도비율은 NaCl 함량에 비례하고 최고 성장률을 나타내는 20%의 $MgSO_4{\cdot}7H_2O$ 배양액에서 가장 높았다. 따라서 배양액내에 $MgSO_4{\cdot}7H_2O$의 함량이 높을수록 phycocyanin과 단백질합성을 촉진한다. 위의 결과는 앞으로 남조식물의 대체식품개발과 의약품의 개발을 위한 새로운 합성물질의 추출에 기여하기 위한 mass culture에 응용될 수 있다고 본다.

• Archives of Pharmacal Research
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• 제20권3호
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• pp.206-211
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• 1997

The optimal conditions for the production and regeneration of the protoplasts from Lentinula edodes were studied. Protoplast formation from the mycelia of L. edodes which were cultured in liquid medium showed a significantly high yield compared with that of the mycelia which were cultured on cellophane covered agar media. A mixture of Novozyme 234 (15 mg/ml) and Cellulase Onozuka R10 (10 mg/ml) in 0.6 M mannitol (pH 4) was optimal lytic enzyme for the protoplast release. The optimal incubation time and mycelia age were 3.5-4 hours at $30^{\circ}C$ and 6-8 days, respectively. Regeneration frequency was 0.18% plated onto a medium containing 0.6 M sucrose, and 0.08% plated onto a medium containing mannitol. But hardly any regeneration was observed in the media containing NaCl, KCl, or $MgSO_{4}$ More than 90% of the protoplasts contained nuclei and the nucleus number per protoplast was 1.1. The DNA content per nucleus was 5.1 pg. The diameter of the protoplast was $3-5{\mu}m$ and it had a well defined cell structure.