Mycobiology

The Korean Society of Mycology (한국균학회)
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Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides

  • Published : 2008.09.30
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Abstract

In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; $K_2HPO_4$, 0.46 g/l; $K_2HPO_4$, 1.00 g/l; M$MgSO_4\cdot7H_2SO$, 0.50 g/l; $FeCl_2\cdot6_2O$, 0.01 g/l; $MnCl_2\cdot4H_2O$, 0.036 g/l; $ZnCl_2$, 0.03 g/l; and $SuSO_4\cdot7H_2O$, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28$^{\circ}C$; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

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References

  1. Berovic, M., Habijanic, J., Zore, I., Wraber, B., Hodzar, D., Boh, B. and Pohleven, F. 2003. Submerged cultivation of Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides. Journal of Biotechnology 103:77-86 https://doi.org/10.1016/S0168-1656(03)00069-5
  2. Brochers, A. T., Stem, J. S., Hackman, R. M., Keen, C. L. and Gershwin, M. E. 1999. Mushrooms, tumors, and immunity. Proc. Soc. Exp. Biol. Med. 221:281-293
  3. Chi, J. H., Ha, T. M., Kim, Y. H. and Ryo Y. D. 1996. Studies on the main factors affecting the myceliall growth of Phellinus linteus. Kor. J. Mycol. 24:214-222
  4. Cho, J. H., Cho, S. D., Hu, H., Kim, S. H., Lee, S. K., Lee Y. S. and Kang K. S. 2002. The role of ERK1/2 and p38 MAP kinases in the prevention mechanism of mushroom Phellinus linteus against the inhibition of gap junction intercellular communication by hydrogen peroxide. Carcinogen 23:1164-1169
  5. Choi, K. H. and Lee, C. W. 2000. Submerged culture of Phellinus linteus in a stirred tank and an airlift fermenter. J. Kor. Ins.Chem. Engin. 38:310-315
  6. Chung, K. S., Kim, H. S., Kim, K. Y., Han, M. W. and Kim, K. H. 1993. Effects of Kp, an antitumor protein polysaccharide from myceliall culture of Phellinus linteus on the humoral immune response of tumor bearing ICR mice to sheep red blood cells. Arch. Pharm. Res. 16:336-338 https://doi.org/10.1007/BF02977527
  7. Han, S. B., Lee, C. W., Jeon, Y. J., Hong, N. D., Yoo, I. D. and Yang, K. H. 1999. The Inhibitory effect of polysaccharides isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacology 41:157-164 https://doi.org/10.1016/S0162-3109(98)00063-0
  8. Hwang, H. J., Kim S. W., Choi, J. W. and Yun, J. W. 2003. Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190. Enzyme Microbiol. Technol. 33:309-319 https://doi.org/10.1016/S0141-0229(03)00131-5
  9. Hwang, H. J., Kim, S. W., Xu, C. P., Choi, J. W. and Yun, J. W. 2004. Morpholoical and rheological properties of the three different species of basidomycetes Phellinus in submerged cultures. J. Appl. Microbiol. 96:1296-1305 https://doi.org/10.1111/j.1365-2672.2004.02271.x
  10. Ikekawa, T., Nakanishi, M., Uehara, N. and Chihara G. 1968. Antitumor action of some basidomycetes, especially Phellinus linteus. Gann. 59:155-157
  11. Kang, T. S., Lee, D. G. and Lee, S. Y. 1997. Isolation and mycelial submerged cultivation of Phellinus sp. Kor. J. Mycol. 25:257-267
  12. Kim, G. Y., Oh, Y. H. and Park, Y. M. 2003a. Acidic polysaccharide isolate from Phellinus linteus induces nitric oxide-mediated tumoricidal activity of macrophages through protein tyrosine kinase and protein kinase C. Biochem. Biophys. Res. Comm. 309:399-407 https://doi.org/10.1016/j.bbrc.2003.08.018
  13. Kim, G. Y., Park, S. K., Lee, M. K., Lee, S. H., Oh, Y. H., Kwak, J. Y., Yoon, S., Lee, J. D. and Park, Y. M. 2003b. Proteoglycan isolated from Phellinus linteus activates murine B lymphocytes via protein kinase C and protein tyrosine kinase. Int. Immunopharmacol. 3:1281-1292 https://doi.org/10.1016/S1567-5769(03)00115-2
  14. Kim, H. M., Han, S. B., Oh, G. T., Kim, Y. H., Hong, D. H., Hong, N. D. and Yoo, I. D. 1996. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int. J. Immunopharmacol. 18:295-303 https://doi.org/10.1016/0192-0561(96)00028-8
  15. Kim, J. L., Kwon, H. K., Chun, G. T., Kim, J. W. and Lee, K. K. 2000. Studies on cultural characteristics for high density fermentation of Phellinus linteus WI-001. Kor. J. Appl. Microbiol. Biotech. 28:105-110
  16. Kim, S. H., Song, Y. S., Kim, S. K., Kim, B. C., Lim, C. J. and Park, E. H. 2004. Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus. J. Enthnopharmacol. 93:141-146 https://doi.org/10.1016/j.jep.2004.03.048
  17. Kim, S. W., Hwang, H. J., Park, J. P., Cho, Y. J., Song, C. H. and Yun, J. W. 2002. Mycelial growth and exo-polymer production by submerged culture of various edible mushrooms under different media. Letters Appl. Microbiol. 34:56-61 https://doi.org/10.1046/j.1472-765x.2002.01041.x
  18. Kues, U. and Liu, Y. 2000. Fruiting body production in basidomycetes. Appl. Microbiol. Biotechnol. 53:141-152
  19. Lee, J. H., Cho, S. M., Ko, K. S. and Yoo, I. D. 1995. Effect of cultural conditions on polysaccharide production and its mononsaccharide composition in Phellinus linteus L13202. Kor. J. Mycol. 23:325-331
  20. Lee, J. W., Baek, S. J., Bae, W. C., Park, J. M. and Kim, Y. S. 2006. Antitumor and antioxidant activities of the extracts from fruiting body of Phellinus linteus. Mycobiology 34:230-235 https://doi.org/10.4489/MYCO.2006.34.4.230
  21. Park, J. B., Kim, S. W., Hwang, H. J. and Yun, J. W. 2001. Optimization of submerged culture conditions for the myceliall growth and exo-polymer production by Cordyceps militaris. Letters Appl. Microbiol. 33:76-81 https://doi.org/10.1046/j.1472-765X.2001.00950.x
  22. Song, K. S., Cho, S. M., Lee, J. H., Kim, H. M., Han, S. B., Ko, K. S. and Yoo, I. D. 1995. B-lymphocyte-stimulating polysaccharide from mushroom Phellinus linteus. Chem. Pharm. Bull. 43:2105-2108 https://doi.org/10.1248/cpb.43.2105
  23. Song, Y. S., Kim, S. H., Sa, J. H., Jin, C. B., Lim, C. J. and Park, E. H. 2003 Anti-angiogenic, antioxidant and xanthine oxidase inhibition activities of the mushoroom Phellinus linteus. J. Enthnopharmacol. 88:113-116 https://doi.org/10.1016/S0378-8741(03)00178-8
  24. Wasser, S. P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulationg polysaccharides. Appl. Microbiol. Biotech. 60:258-274 https://doi.org/10.1007/s00253-002-1076-7
  25. Zhong, J. J. and Tang, Y. J. 2004. Submerged cultivation of medicinal mushrooms for production of valuable bioactive metabolites. Adv. Biochem. Engin. Biotech. 87:25-59

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