• Title/Summary/Keyword: optical density

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Controlling activity of Bion-M against bacterial spot caused by Xanthomonas euvesicatoria (고추 세균점무늬병에 대한 Bion-M의 방제 효과)

  • Kim, A-Hyeong;Yeon, Cho-Long;Kim, Joo-Hyung;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.16 no.2
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    • pp.171-177
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    • 2012
  • Pepper seedlings of 6-leaf stage were inoculated with cell suspension of Xanthomonas euvesicatoria 1523 7 days after the application of Bion-M by soil-drenching. Disease severity in the treatment with 20 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, with which Bion-M was composed, was 19%, whereas that of the untreated control was 75%. The resulting control value of acibenzolar-S-methyl was calaulated as much as 74.7%. The control value of acibenzolar-S-methyl was dependent with the applied concentrations and ranged 29.3% to 49.3% at 4.0 and 0.8 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl, respectively. Phytotoxicity was observed at 20.0 ${\mu}g\;mL^{-1}$, as lower leaves became to be yellowed and defoliated. The cell density of inoculum suspension of X. euvesicatoria 1523 affected the control value of acibenzolar-S-methyl. With optical density (O.D.) of 0.5 the control value of 4.0 ${\mu}g\;mL^{-1}$ of acibenzolar-S-methyl was 86.0%. However, the control value improved as high as 97.8% at the O.D. value of 0.1. The control value was 75.0% in adult plant of pepper, when acibenzolar-S-methyl was treated by soil-drenching 7 days before inoculation with cell suspension of X. euvesicatoria 1523. The control effect of acibenzolar-S-methyl on pepper bacterial spot was obtained in pepper field, showing that the control value at 10.0 ${\mu}g\;mL^{-1}$ was 71.2%.

Growth and optical conductivity properties for BaIn2S4 single crystal thin film by hot wall epitaxy (Hot Wall Epitaxy(HWE)법에 의한 BaIn2S4 단결정 박막 성장과 광전도 특성)

  • Jeong, Kyunga;Hong, Kwangjoon
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.5
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    • pp.173-181
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    • 2015
  • A stoichiometric mixture of evaporating materials for $BaIn_2S_4$ single crystal thin films was prepared from horizontal electric furnace. To obtain the single crystal thin films, $BaIn_2S_4$ mixed crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperatures were $620^{\circ}C$ and $420^{\circ}C$, respectively. The crystalline structure of the single crystal thin films was investigated by double crystal X-ray diffraction (DCXD). The carrier density and mobility of $BaIn_2S_4$ single crystal thin films measured from Hall effect by van der Pauw method are $6.13{\times}10^{17}cm^{-3}$ and $222cm^2/v{\cdot}s$ at 293 K, respectively. The temperature dependence of the energy band gap of the $BaIn_2S_4$ obtained from the absorption spectra was well described by the Varshni's relation, $E_g(T)=3.0581eV-(3.9511{\times}10^{-3}eV/K)T^2/(T+536K)$. The crystal field and the spin-orbit splitting energies for the valence band of the $BaIn_2S_4$ have been estimated to be 182.7 meV and 42.6 meV, respectively, by means of the photocurrent spectra and the Hopfield quasicubic model. These results indicate that the splitting of the ${\Delta}so$ definitely exists in the ${\Gamma}_5$ states of the valence band of the $BaIn_2S_4/GaAs$ epilayer. The three photocurrent peaks observed at 10 K are ascribed to the $A_1$-, $B_1$-exciton for n = 1 and $C_{24}$-exciton peaks for n = 24.

Effects of chromium chloride addition on coloration and mechanical properties of 3Y-TZP (크롬염화물 첨가에 따른 지르코니아 색상 및 물리적 성질 변화에 관한 연구)

  • Oh, Gye-Jeong;Seo, Yoon-Jeong;Yun, Kwi-Dug;Lim, Hyun-Pil;Park, Sang-Won;Lee, Kyung-Ku;Lim, Tae-Kwan;Lee, Doh-Jae
    • The Journal of Korean Academy of Prosthodontics
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    • v.49 no.2
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    • pp.120-127
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    • 2011
  • Purpose: The purpose of this study was to examine the effects of chromium chloride addition on coloration, mechanical property and microstructure of 3Y-TZP. Materials and methods: Chromium chloride was weighed as 0.06, 0.12, and 0.25 wt% and each measured amount was dissolved in alcohol. $ZrO_2$ powder was mixed with each of the individual slurry to prepare chromium doped zirconia specimen. The color, physical properties and microstructure were observed after the zirconia specimen were sintered at $1450^{\circ}C$. In order to evaluate the color, spectrophotometer was used to analyze the value of $L^*$, $C^*$, $a^*$ and $b^*$, after placing the specimen on a white plate, and measured according to the International Commission on Illumination (CIE) standard, Illuminant D65 and SCE system. The density was measured in the Archimedes method, while microstructures were evaluated by using the scanning electron microscopy (SEM) and XRD. Fracture toughness was calculated Vickers indentation method and indentation size was measured by using the optical microscope. The data were analyzed with 1-way ANOVA test (${\alpha}$ = 0.05). The Tukey multiple comparison test was used for post hocanalysis. Results: 1. Chromium chloride rendered zirconia a brownish color. While chromium chloride content was increased, the color of zirconia was changed from brownish to brownish-red. 2. Chromium chloride content was increased; density of the specimen was decreased. 3. More chromium chloride in the ratio showed increase size of grains. 4. But the addition of chromium chloride did not affect the crystal phase of zirconia, and all specimens showed tetragonal phase. 5. The chromium chloride in zirconia did not showed statistically significant difference in fracture toughness, but addition of 0.25 wt% showed a statistically significant difference (P<.05). Conclusion: Based on the above results, this study suggests that chromium chlorides can make colored zirconia while adding in a liquid form. The new colored zirconia showed a slight difference in color to that of the natural tooth, nevertheless this material can be used as an all ceramic core material.

Effects of Optical Characteristics on the Growth of Benthic Microalga, Nitzschia sp. and Its Growth Kinetics of Phosphate for Bioremediation (생물적 환경정화를 위한 부착미세조류 Nitzschia sp.의 생장에 미치는 광학적 특성과 그에 따른 인산염 성장 동력학)

  • Oh, Seok-Jin;Kang, In-Seok;Yoon, Yang-Ho;Yang, Han-Soeb;Park, Jong-Sick
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.14 no.4
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    • pp.205-212
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    • 2009
  • To suggest possible to bioremediation by benthic microalgae Nitzschia sp. isolated from the Jinhae Bay, the studies investigated the effects o flight quality and quantity on the growth of Nitzschia sp. and its growth kinetics for phosphate investigated. The Nitzschia sp. was cultured under blue (450 nm), yellow (590 nm) and red wavelength (650 nm) using light emitting diode (LED) and mixed wavelengths using a fluorescent lamp. The maximum specific growth rate showed the Nitzschia sp. under blue wavelength, although photoinhibition was observed above $100\;{\mu}mol\;m^{-2}\;s^{-1}$. Mixed wavelengths were also observed by decreasing the maximum cell density from high irradiances (>$100\;{\mu}mol$ photons $m^{-2}\;s^{-1}$). The compensation photon flux density ($I_0$) calculated from the mixed wavelengths equated to a depth of 4-10 m in Jinhae Bay, and was lower in the summer season than the depth due to suspended matter (ca. 4 m). Thus, the suitable depth for maximum growth of Nitzschia sp. might be extremely limited. In the growth kinetics for phosphate, half-saturation constant ($K_s$) was similar among different wavelengths, although the maximum growth rate was varied among different wavelengths. Because the $K_s$ was high than that of the phytoplankton, Nitzschia sp. might have adapted to the high nutrient concentrations, and have effective nutrient storage in the cell quota. Thus, Nitzschia sp. may be a useful species for bioremediation of the benthic layer in polluted inner bays by means of irradiated specific wavelength as blue.

Development of a Solar Collector Performance of Cylindrical Parabolic Concentrating Solar Collector (태양열(太陽熱) 집열기개발(集熱器開發)에 관(關)한 연구(硏究) - 포물반사곡면(抛物反射曲面)으로된 2차원(二次元) 집광식(集光式) 태양열(太陽熱) 집열기(集熱器)의 성능분석(性能分析) -)

  • Song, Hyun Kap;Yon, Kwang Seok;Cho, Sung Chan
    • Journal of Biosystems Engineering
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    • v.10 no.1
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    • pp.54-68
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    • 1985
  • It is desirable to collect the solar thermal energy at relatively high temperature in order to minimize the size of thermal storage system and to enlarge the scope of solar thermal energy utilization. So far the concentrating solar collector has been developed to collect solar thermal energy at relatively high temperature, but it has some difficulties in maintaining the volumetric body of solar collector for long term utilization. On the other hand, the flat-plate solar collector has been developed to collect the solar thermal energy at low temperature, and it has advantages in maintaining the system for long term utilization, since it's thickness is thin and not volumetric. In this study, to develop a solar collector that has both advantages of collecting solar thermal energy at high temperature and fixing conveniently the collector system for long term period, a cylindrical parabolic concentrating solar collector was designed, which has two rows of parabolic reflectors and thin thickness such as the flat-plate solar collector, maintaining the optical form of concentrating solar collector. The characteristics of the concentrating parabolic solar collector newly designed was analysed and the results are summarized as follows; 1. The temperature of the air enclosed in solar collector was all the same as $50^{\circ}C$ in both cases of the open and closed loop, and when the heat transfer fluid was not circulated in tubular absorber, the maximum surface temperature of the absorber was $118-120^{\circ}C$, this results suggested that the heat transfer fluid could be heated up to $118^{\circ}C$. 2. In case of longitudinal installation of the solar collector, the temperature difference of heat transfer fluid between inlet and outlet was $4^{\circ}-6^{\circ}C$ at the flow rate of $110-130{\ell}/hr$, and the collected solar energy per unit area of collector was $300-465W/m^2$. 3. The collected solar energy per unit area for 7 hours was 1960 Kcal/$m^2$ for the open loop and 220 Kcal/$m^2$ for the closed loop. Therefore it is necessary to combine the open and closed loop of solar collectors to improve the thermal efficiency of solar collector. 4. The thermal efficiency of the solar collector (C.P.C.S.C.) was proportional to the density of solar radiation, indicating the maximum thermal efficiency ${\eta}_{max}=58%$ with longitudinal installation and ${\eta}_{max}=45%$ with lateral installation. 5. The thermal efficiency of the solar collector (C.P.C.S.C.) was increased in accordance with the increase of flow rate of heat transfer fluid, presenting the flow rate of $110{\ell}/hr$ was the value of turning point of the increasing rate of the collector efficiency, therefore the flow rate of $110{\ell}/hr$ was considered as optimum value for the test of the solar collector (C.P.C.S.C.) performance when the heat transfer fluid is a liquid. 6. In both cases of longitudinal and lateral installation of the solar collector (C.P.C.S.C.), the thermal efficiency was decreased linearly with an increase in the value of the term ($T_m-T_a$)/Ic and the increasing rate of the thermal efficiency was not effected by the installation method of solar collector.

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Evaluation of Lipid Peroxidation Inhibition and Nitrogen Oxide Scavenging Activity from Peel of Gardenia jasminoides Ellis Fructus Extracted by Various Solvents (치자(Gardenia jasminoides Ellis fructus) 껍질 용매 별 추출물의 지질과산화 저해 및 질소산화물 소거능)

  • Jin, Dong-Hyeok;Oh, Da-Young;Chung, Hun-Sik;Lee, Young-Guen;Seong, Jong-Hwan;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.244-253
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    • 2017
  • The aim of this study was to investigate the bioactivity and antioxidant activity of peel from Gardenia jasminoides Ellis fructus (GJE). We were separated into GJE peel. After that, we determined anthocyanin. GJE peel were extracted by 70% methanol, distilled water (DW) and ethyl acetate (EA) three solvents. To investigate by the solvent extract of total phenol content and value as a functional food ingredient of GJE peel through nitrogen oxide scavenging activity, antioxidant activity, reducing power and lipid peroxidation inhibition were performed. Solvent extract bioactivity of increasing concentrations (0.2, 0.4, 0.6 mg/mL) were significantly increased (p<0.05). GJE peel extracts showed lower activity than positive control (ascorbic acid, BHA, trolox). The total phenol contents of GJE peel extracts were highest in EA extract. However, the order of total phenol content of the solvent in the GJE peel and the results of analysis of various physiological activities were inconsistent. Considering the extraction yield and various physiological activities, it is expected to be effective when extracted from 70% methanol and DW extract. The results suggest that GJE peel is highly expected to be useful as a functional foods and natural antioxidant.

Dose Evaluation at The Build Up Region Using by Wedge Filter (쐐기필터 사용에 따른 선량증가 영역에서 선량평가)

  • Kim, Yon-Lae;Moon, Seong-Kong;Suh, Tae-Suk;Chung, Jin-Beom;Kim, Jin-Young;Lee, Jeong-Woo
    • Journal of radiological science and technology
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    • v.37 no.4
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    • pp.341-348
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    • 2014
  • Wedge filter could use to increase the dose distribution at the hot dose regions. We evaluated dose discrepancy at surface and build region in the infield and outfield that Metal Wedge (MW) and Enhance Dynamic Wedge (EDW) were interact with photon. In this paper, we used Gafchromic EBT3 film that had excellent spatial resolution, composed the water equivalent materials and changed the optical density without development. The set up conditions of linear accelerator were fixed 6 MV photon, 100 cm SSD, $10{\times}10cm^2$ field size and were irradiated 400 cGy at Dmax. The dose distribution and absorbed dose were evaluated when we compared the open field with $15^{\circ}$, $30^{\circ}$, $45^{\circ}$ metal wedge and enhanced dynamic wedge. A $15^{\circ}$ metal wedge could increase the surface and build up region dose than using a $15^{\circ}$ enhanced dynamic wedge. A $30^{\circ}$ metal wedge could decrease the surface and build up region dose than using a $30^{\circ}$ enhanced dynamic wedge. A $45^{\circ}$ metal wedge could decrease by large deviation the surface and build up region dose than using a $15^{\circ}$ enhanced dynamic wedge. The dose of penumbra region at outfield were increased on the thick side but were decreased on the thin side. It could be decrease the surface dose and build up region dose, if the metal wedge filters were properly used to make a good dose distribution and not closed the distance of surface.

Optimization of fractionation efficiency (FE) and throughput (TP) in a large scale splitter less full-feed depletion SPLITT fractionation (Large scale FFD-SF) (대용량 splitter less full-feed depletion SPLITT 분획법 (Large scale FFD-SF)에서의 분획효율(FE)및 시료처리량(TP)의 최적화)

  • Eum, Chul Hun;Noh, Ahrahm;Choi, Jaeyeong;Yoo, Yeongsuk;Kim, Woon Jung;Lee, Seungho
    • Analytical Science and Technology
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    • v.28 no.6
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    • pp.453-459
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    • 2015
  • Split-flow thin cell fractionation (SPLITT fractionation, SF) is a particle separation technique that allows continuous (and thus a preparative scale) separation into two subpopulations based on the particle size or the density. In SF, there are two basic performance parameters. One is the throughput (TP), which was defined as the amount of sample that can be processed in a unit time period. Another is the fractionation efficiency (FE), which was defined as the number % of particles that have the size predicted by theory. Full-feed depletion mode (FFD-SF) have only one inlet for the sample feed, and the channel is equipped with a flow stream splitter only at the outlet in SF mode. In conventional FFD-mode, it was difficult to extend channel due to splitter in channel. So, we use large scale splitter-less FFD-SF to increase TP from increase channel scale. In this study, a FFD-SF channel was developed for a large-scale fractionation, which has no flow stream splitters (‘splitter less’), and then was tested for optimum TP and FE by varying the sample concentration and the flow rates at the inlet and outlet of the channel. Polyurethane (PU) latex beads having two different size distribution (about 3~7 µm, and about 2~30 µm) were used for the test. The sample concentration was varied from 0.2 to 0.8% (wt/vol). The channel flow rate was varied from 70, 100, 120 and 160 mL/min. The fractionated particles were monitored by optical microscopy (OM). The sample recovery was determined by collecting the particles on a 0.1 µm membrane filter. Accumulation of relatively large micron sized particles in channel could be prevented by feeding carrier liquid. It was found that, in order to achieve effective TP, the concentration of sample should be at higher than 0.4%.

THE INHIBITORY EFFECT OF STREPTOCOCCUS SALIVARIUS 119 ON THE FORMATION OF ARTIFICIAL PLAQUE (Streptococcus salivarius 119의 인공치태 억제효과에 대한 연구)

  • Lee, Min-Ha;Yang, Kyu-Ho;Oh, Jong-Suk
    • Journal of the korean academy of Pediatric Dentistry
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    • v.27 no.1
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    • pp.15-23
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    • 2000
  • Streptococcus salivarius is a normal inhabitant in the human oral cavity. Streptococcus salivarius 119 in this study was isolated from the oral cavity of child and identified, and its action mechanism on the formation of denal plaque by Streptococcus matans was studied. 1. The optical density of absorbance at 550 nm was 0.327 in the culture of Streptococcus mutans in disposable cuvette, whereas being 0.119 in the combined culture of Streptococcus mutans and Streptococcus salivarius 119. 2. The mean weight of produced artificial plaque on the wires in the beaker was 84.7mg in culture of Streptococcus mutans only, whereas being reduced to 12.3mg in the combined culture of Streptococcus mutans and Streptococcus salivarius 119. 3. When Streptococcus mutans was cultured in the media containing culture supernatant of Streptococcus salivarius 119 in BHI broth, the mean weight of produced artificial plaque was 100.5mg on the wires, whereas being reduced to 20.4mg in the media containing culture supernatant of Streptococcus salivarius 119 in BHIS broth. 4. The viable cells of Streptococcus oralis and Streptococcus salivarius 119 were $4.8\times10^7\;and\;7.5\times10^8$ per ml respectively after each culture, wheras being $4.2\times10^7\;and\;5.8\times10^7$ per ml in the combined culture of Streptococcus oralis and Streptococcus salivarius 119. 5. The polymer produced by Streptococcus salivarius 119 was glucan on the thin layer chromatography. 6. The glucan produced by Streptococcus salivarius 119 was water-soluble glucan containing $1\rightarrow6$ linkages as the main linkage on the thin layer chromatography. These results suggested that isolated Streptococcus salivarius 119 inhibited the formation of artificial plaque by the production of water-soluble glucan.

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Specific antibody response of olive flounder Paralichthys olivaceus by water temperature (수온에 따른 넙치의 특이 항체 반응)

  • Kim, Wi-Sik;Jang, Min-Seok;Jung, Sung-Ju;Kim, Seok-Ryel;Park, Myoung-Ae;Lee, Jeong-Ho;Myeong, Jeong-In;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.24 no.1
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    • pp.39-45
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    • 2011
  • The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of $15^{\circ}C$, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24~48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94~3.04. At the end of the experiment (84 days), 0.03~1.28 OD values were observed. In the rearing temperature of $12{\sim}13^{\circ}C$, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88~2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of $10^{\circ}C$, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37~1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.