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http://dx.doi.org/10.7847/jfp.2011.24.1.039

Specific antibody response of olive flounder Paralichthys olivaceus by water temperature  

Kim, Wi-Sik (The Fisheries Science Institute, Chonnam National University)
Jang, Min-Seok (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Jung, Sung-Ju (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Kim, Seok-Ryel (Pathology Division, National Fisheries Research and Development Institute)
Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute)
Lee, Jeong-Ho (Genetics & Breeding Research Center, National Fisheries Research & Development Institute)
Myeong, Jeong-In (Genetics & Breeding Research Center, National Fisheries Research & Development Institute)
Oh, Myung-Joo (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University)
Publication Information
Journal of fish pathology / v.24, no.1, 2011 , pp. 39-45 More about this Journal
Abstract
The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of $15^{\circ}C$, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24~48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94~3.04. At the end of the experiment (84 days), 0.03~1.28 OD values were observed. In the rearing temperature of $12{\sim}13^{\circ}C$, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88~2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of $10^{\circ}C$, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37~1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.
Keywords
Antibody detection; Enzyme-linked immunosorbent assay; Olive flounder; Water temperature;
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