• The Journal of Korean Physical Therapy
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• v.31 no.4
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• pp.161-168
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• 2019

Most athletes with anterior cruciate ligament (ACL) ruptures undergo a surgical ACL reconstruction (ACLR) and rehabilitation. On the other hand, controversy still exists because neither a reconstruction nor rehabilitation have been proven to be superior in the management of ACL injury. This study reviewed the success rates of interventions to provide recommendations for the optimal management after an ACL injury. One of the most important considerations after an ACL injury is the timing and type of intervention. At the early stages, which involve the loss of volume and strength of quadriceps femoral muscle, weight bearing (closed kinetic chain) exercises with pain management followed by high velocity resistance exercises in an open kinetic chain environment are recommended to improve the quadriceps function. After that, it is important to apply intensive isokinetic exercise with a lower extension rate. In this case, it is important to apply overload to the muscles and to simultaneously lead the co-contraction of the hamstrings. Standards are essential because the timing and type of interventions are crucial to prevent re-injury and complications, such as osteoarthritis, as well as to confirm the successful outcome of the treatment. Different interventions recommended for ACL damage have yet to reach consensus. Further studies will be needed to observe the effects of the intervention through multidisciplinary approaches.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.15 no.12
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• pp.4400-4419
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• 2021

In the 4th Industrial Revolution, the healthcare industry is undergoing a paradigm shift from post-care and management systems based on diagnosis and treatment to disease prevention and management based on personal precision medicine. To optimize medical services for individual patients, an open ecosystem for the healthcare industry that allows the exchange and utilization of personal health records (PHRs) is required. However, under the current system of hospital-centered data management, it is difficult to implement the linking and sharing of PHRs in practice. To address this problem, in this study, we present the design and implementation of a patient-centered PHR platform using blockchain technology. This platform achieved transparency and reliability in information management by eliminating the risk of leakage and tampering/altering personal information, which could occur when using a PHR. In addition, the patient-consent system was applied to a PHR; thus, the patient acted as the user with ownership. The proposed blockchain-based PHR platform enables the integration of personal medical information with scattered distribution across multiple hospitals, and allows patients to freely use their health records in their daily lives and emergencies. The proposed platform is expected to serve as a stepping stone for patient-centered healthcare data management and utilization.

• Journal of Marine Life Science
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• v.3 no.1
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• pp.1-8
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• 2018

Myosin is considered as the vital motor protein in vertebrates and invertebrates. Our present study was conducted to decipher the occurrence of myosin in dog fish (Squalus mitsukurii). We isolated one clone containing 979 bp cDNA sequence, which consisted of a complete coding sequence of 453 bp and a deduced amino acid sequence of 150 amino acids from the open reading frame with molecular weight, isoelectric point and aliphatic index are 16.72 Kda, 4.49 and 78.00, respectively. It contained 428 bp long 3' UTR with single potential polyadenylation signals (AATAAA). The predicted EF CA²⁺ binding domains were identified in residue 6-41, 83-118 and 133-150. A BLAST search indicates this protein exhibits a strong similarity to whale shark (Rhincodon typus) MLC3 (91% identical) and also house mouse (Mus musculus) MLC isoform 3f (81% identical). Phylogenetic analysis revealed that this protein is a MLC 3 isoform like protein. This protein also demonstrates highly conserved region with other myosin proteins. Homology modeling of S. mitsukuri was performed using crystal structure of Gallus gallus skeletal muscle myosin II based on high similarity. Reverse transcription-polymerase chain reaction (PCR), quantitative PCR results exhibits dogfish myosin protein is highly expressed in muscle tissue.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1189-1196
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• 2005

The cyclohexanol dehydrogenase (ChnA), produced by Rhodococcus sp. TK6, which is capable of growth on cyclohexanol as the sole carbon source, has been previously purified and characterized. However, the current study cloned the complete gene (chnA) for ChnA and its flanking regions using a combination of a polymerase chain reaction (PCR) based on the N-terminal amino acid sequence of the purified ChnA and plaque hybridization from a phage library of Rhodococcus sp. TK6. A sequence analysis of the 5,965-bp DNA fragment revealed five potential open reading frames (ORFs) designated as partial pte (phosphotriesterase), acs (acyl-CoA synthetase), scd (short chain dehydrogenase), stp (sugar transporter), and chnA (cyclohexanol dehydrogenase), respectively. The deduced amino acid sequence of the chnA gene exhibited a similarity of up to $53\%$ with members of the short-chain dehydrogenase/reductase (SDR) family. The chnA gene was expressed using the pET21 a(+) system in Escherichia coli. The activity of the expressed ChnA was then confirmed (13.6 U/mg of protein) and its properties investigated.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.467-472
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• 2018

Objective: Molecular cloning and bioinformatics analysis of annexin A2 (ANXA2) gene in sika deer antler tip were conducted. The role of ANXA2 gene in the growth and development of the antler were analyzed initially. Methods: The reverse transcriptase polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the ANXA2 gene from antler tip of sika deer (Cervus Nippon hortulorum) and the bioinformatics methods were applied to analyze the amino acid sequence of Anxa2 protein. The mRNA expression levels of the ANXA2 gene in different growth stages were examined by real time reverse transcriptase polymerase chain reaction (real time RT-PCR). Results: The nucleotide sequence analysis revealed an open reading frame of 1,020 bp encoding 339 amino acids long protein of calculated molecular weight 38.6 kDa and isoelectric point 6.09. Homologous sequence alignment and phylogenetic analysis indicated that the Anxa2 mature protein of sika deer had the closest genetic distance with Cervus elaphus and Bos mutus. Real time RT-PCR results showed that the gene had differential expression levels in different growth stages, and the expression level of the ANXA2 gene was the highest at metaphase (rapid growing period). Conclusion: ANXA2 gene may promote the cell proliferation, and the finding suggested Anxa2 as an important candidate for regulating the growth and development of deer antler.

• Polymer(Korea)
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• v.26 no.6
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• pp.759-766
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• 2002

Regular, spherical and isotropic open-microcellular foams having low density were prepared by the high internal phase emulsion (HIPE) polymerization mainly composed of styrene monomer and water The effects of Polymerization conditions. such as the content of water, divinylbenzene as a crosslinking agent and dodecane as a chain transfer agent, were investigated based on the tell size and foam properties. The microstructural morphology was observed using scanning electron microscopy (SEM) and the compression modulus of the foam was evaluated using compression test. The dropwise feeding of the aqueous phase into the oil phase was more effective than the batch feeding in producing the uniform and stable foam. Agitation speed and surfactant strongly influenced on the cell size and the window size between water droplets. Introduction of chain transfer agent increased the cell size, whereas it decreased the window size. Compression modulus increased with the crosslinking agent, but decreased with the chain transfer agent.

• Journal of Digital Convergence
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• v.17 no.3
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• pp.215-220
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• 2019

The block chain technology is a technique that prevents manipulation of data and ensures integrity and reliability. Ethereum is building a smart contract environment as a type of encryptionenabled system based on block chains. Smart contracts can be implemented when conditions are met, thus increasing confidence in digital data. However, smart contracts that are being tried in various ways are not covered by information security and personal information protection. The structure in which the network participant can view the open transaction ledger is exposed to data or personal information listed in the block chain. In this study, it is possible to manage the data of personal information recorded in the block chain directly. This study is protected personal information by preventing the exposure of personal information and by executing time code, it is possible to erase recorded information after a certain period of time has elapsed. Based on the proposed system in the future, it is necessary to study the additional management techniques of unknown code defects or personal information protection.

• The Journal of the Convergence on Culture Technology
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• v.5 no.1
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• pp.451-456
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• 2019

Our students are shocked by the results that there is an inverse proportion between academic achievement and life satisfaction. Recently, the Organization for Economic Cooperation and Development (OECD) published the PISA 2015 Student Well-Being Report. According to the report, Korean students' education is the highest among OECD countries, but their life satisfaction is the 47th ranked among the 48 OECD and non-OECD countries.[1] This is the cause of the unilateral education using collective achievement in evaluation methods. Therefore, I propose a personalized integrated model utilizing block chain technology. I suggest a personalized education and evaluation system model using selective education and personal approval rather than from teachers' unilateral education. This will no longer open the student's achievement grade system to the public. This change will be accomplished utilizing block chain technology. This technology is characterized by security, transparency and dispersion. The result of this work can be used to improve students' quality of living through a management system of personalized education and evaluation, based on the nature of education.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.19 no.1
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• pp.93-105
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• 2024

Car-sharing is a representative model of the sharing economy, and it is a service that rents or uses a car for the necessary time without owning a car. This industry is growing due to various factors such as technological advances, increasing awareness of environmental protection, and increasing demand for solving traffic congestion problems in cities. Accordingly, there is a need for a strategic approach for companies providing car-sharing services to respond quickly to market changes in order to expand market share and differentiate services. Accordingly, this study conducted a case study on open innovation activities between Gongcar and existing rental car companies, focusing on the research question "What effects do open innovation activities between car-sharing companies and existing rental car companies cause?" As a result of the study, it was confirmed that Gongcar have (1) the ability to actively respond to market fluctuations by establishing a flexible vehicle supply chain based on demand, (2) have significantly reduced growth capital expenditure (Growth Capex), and both cafe and rental car companies have (3) performed successful open innovation by improving key KPI indicators and recording financial performance. This study reveals how open innovation acts as a key business growth engine in the car-sharing industry, and its significance is found in that it empirically confirmed the successful implementation conditions of open innovation based on resource dependence theory.

• Applied Biological Chemistry
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• v.39 no.2
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• pp.112-117
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• 1996

Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of diamine putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from Escherichia coli, oat and tomato genome. Two degenerate oligonucleotides (17-mer) corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction of rice (Oryza sativa L.) genomic DNA, and an approximately 1.0 kbp fragment was obtained. This amplified PCR product showed an open reading frame which contains 1,022 bp of nucleotide sequences. This PCR product was cloned into pGEM-originated T vector and the short 500 bp PstI digested fragment was subcloned into pGEM-3zf(+/-) vectors to facilitate sequencing. The nucleotide sequence of this PCR product showed about 74% and 70% identity with the same regions of the oat and tomato ADC cDNA sequences, respectively. The predicted amino acid sequence exhibited 45% and 62% identity with oat and tomato ADC polypeptide fragments, respectively. The sequence similarities of 34%, 47% and 38% were previously reported in oat and E. coli, tomato and oat, and tomato and E. coli ADC amino acids, respectively. Therefore, similarities and identities between rice and oat or tomato are remarkably higher than those others of the previous reports. In the highly conserved regions in both the amino acid sequence and spacing regions among the sequences of these three, rice ADC open reading frame also has the exactly same regions with the striking similarity. RNA blot analysis showed that hnc is expressed as a transcript of approximately 2.5 kbP in the rice seedling leaf tissues.