• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.667-672
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• 2019

We compared two integration systems for stable expression of heterologous genes in Saccharomyces cerevisiae. A Candida glabrata-derived gene was used as the selective marker for the Cre/loxP system, and XYLP, XYLB, GRE3, and XYL2 genes were used as model heterologous genes and ligated into the universal pRS-CMT vector. The resulting pRS-XylP, pRS-XylB, pRS-Gre3, and pRS-Xyl2 plasmids were sequentially integrated into yeast chromosome VII by four integration processes (marker rescue and gene integration). The four introduced genes were successfully expressed. Further, the pRS-PBG2 plasmid harboring expression cassettes for the four genes was constructed for one-step integration. The four genes that were introduced were stably maintained as a gene cluster and were simultaneously expressed. The one-step integration was more effective for the simultaneous integration and expression of the four genes related to xylan/xylose metabolism. This method will enable the generation of a useful biosystem through appropriate use of gene integration methods.

• Transactions of the Korean Society of Automotive Engineers
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• v.4 no.4
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• pp.190-200
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• 1996

Step-by-step time integration methods are widely used for solving structural dynamics problem. One difficult yet critical choice an analyst must make is to decide an appropriate time step size. The choice of time step size has a significant effect on solution accuracy and computational expense. The objective of this research is to derive error estimate for newly developed time integration method and develop automatic time step size control algorithm for structural dynamics. A formula for computing error tolerance is derived based on desired period resolution. An automatic time step size control strategy is proposed based on a normalized local error estimate for the generalized-α method. Numerical examples demonstrate the developed strategy satisfies general design criteria for time step size control algorithm for dynamic problem.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.6
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• pp.573-578
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• 2010

Precise determination of satellite positions is necessary to improve positioning accuracy in GNSS. In this study, GLONASS orbits were predicted from broadcast ephemeris using the 4th-order Runge-Kutta numerical integration method and their accuracy dependence on the integration step and the integration time was analyzed. The 3D RMS (Root Mean Square) differences between the results from I-second integration step and 300-second integration step was about 3 cm, but the processing time was one hundred times less for the I-second integration time case. For trials of different integration times, the 3D RMS errors were 8.3 m, 187.3 m, and 661.5 m for 30-, 150-, and 300-minutes of integration time, respectively. Though this integration-time analysis, we concluded that the accuracy gets higher with a shorter integration time. Thus we suggest forward and backward integration methods to improve GLONASS positioning accuracy, and with this method we can achieve a 5-meter level of 3-D orbit accuracy.

• Proceedings of the Korean Statistical Society Conference
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• 2003.05a
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• pp.269-273
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• 2003

We consider an efficient parametric estimation method of spatial dependence in weak stationary processes. Spatial dependence is modeled through variogram and correlogram. Most of parametric estimation methods of correlogram use two step method; nonparametric estimation and parametric integration. We bind these two steps into one step by using GEE method instead of least squares type optimization. Our one step method is more efficient statistically and gives a clear interpretation of related concepts used in traditional two step methods.

• Transactions of the Korean Society of Mechanical Engineers
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• v.18 no.6
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• pp.1455-1464
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• 1994

A new and efficient algorithm for the integration of the thermal-elasto-plastic constitutive equation is proposed. While it falls into the category of the return mapping method, the algorithm adopts the three point approximation of plastic corrector within one time increment step. The results of its application to a von Mises-type thermal-elasto-plastic model with combined hardening and temperature-dependent material properties show that the accurate iso-error maps are obtained for both angular and radial errors. The accuracy achieved is because the predicted stress increment in a single step calculation follows the exact value closely not only at the end of the step but also through the whole path. Also, the comparison of the computational time for the new and other algorithms shows that the new one is very efficient.

• 한국기계연구소 소보
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• s.18
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• pp.55-66
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• 1988

Many direct integration methods are used for numerical analyses of dynamic motion. In these methods, the governing equations of a dynamic system are integrated successively using a step-by-step numerical integration procedure. Time derivatives in the equations are generally approximated using difference formulas involving one or more increments of the time. Time increment has closely relationship with the accuracy of the motion analysis. In this paper, a 4th order Houbolt direct integration method is derived. For a spring-mass system, the motion of the system are analyzed from the 3rd order Houbolt and the 4th order Houbolt approaches respectively. Finally the paper proposes the optimal time-increment based on the accuracy of numerical analyses.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.11
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• pp.1111-1117
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• 2008

During the dynamic analysis of a system, the Coulomb friction law is emploved to calculate the friction force. Since the static friction coefficient is only employed during the zero relative velocity, it is impractical to employ the coefficient during the dynamic analysis. To calculate the static friction force, therefore, some friction models have been developed. In this study, the integration stability and the accuracy of the models are investigated with some numerical examples. The effect of time step size during the numerical integration is also investigated. The numerical study shows that the friction model employed for most commercial codes is not as good as the one proposed in this study.

• Structural Engineering and Mechanics
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• v.22 no.6
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• pp.701-717
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• 2006

In the existing reports regarding free transverse vibrations of the Euler-Bernoulli beams, most of them studied a uniform beam carrying various concentrated elements (such as point masses, rotary inertias, linear springs, rotational springs, spring-mass systems, ${\ldots}$, etc.) or a stepped beam with one to three step changes in cross-sections but without any attachments. The purpose of this paper is to utilize the numerical assembly method (NAM) to determine the exact natural frequencies and mode shapes of the multiple-step Euler-Bernoulli beams carrying a number of lumped masses and rotary inertias. First, the coefficient matrices for an intermediate lumped mass (and rotary inertia), left-end support and right-end support of a multiple-step beam are derived. Next, the overall coefficient matrix for the whole vibrating system is obtained using the numerical assembly technique of the conventional finite element method (FEM). Finally, the exact natural frequencies and the associated mode shapes of the vibrating system are determined by equating the determinant of the last overall coefficient matrix to zero and substituting the corresponding values of integration constants into the associated eigenfunctions, respectively. The effects of distribution of lumped masses and rotary inertias on the dynamic characteristics of the multiple-step beam are also studied.

• Kyungpook Mathematical Journal
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• v.56 no.2
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• pp.311-327
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• 2016

In this paper, we propose an error embedded Runge-Kutta method to improve the traditional embedded Runge-Kutta method. The proposed scheme can be applied into most explicit embedded Runge-Kutta methods. At each integration step, the proposed method is comprised of two equations for the solution and the error, respectively. These solution and error are obtained by solving an initial value problem whose solution has the information of the error at each integration step. The constructed algorithm controls both the error and the time step size simultaneously and possesses a good performance in the computational cost compared to the original method. For the assessment of the effectiveness, the van der Pol equation and another one having a difficulty for the global error control are numerically solved. Finally, a two-body Kepler problem is also used to assess the efficiency of the proposed algorithm.

• Structural Engineering and Mechanics
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• v.60 no.5
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• pp.891-902
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• 2016

The beam structure with breathing cracks subjected to harmonic excitations was modeled by FEM based on Euler-Bernoulli theory, and a piecewise dynamical system was deduced. The precise integration method (PIM) was employed to propose an algorithm for analyzing the dynamic responses of the deduced system. This system was first divided into linear sub-systems, between which there are switching points resulted from the breathing cracks. The inhomogeneous terms due to the external excitations were tackled by introducing auxiliary variables to express the harmonic functions, hence the sub-systems are homogeneous. The PIM was then applied to solve the homogeneous sub-systems one by one. During the procedures, a predictor-corrector algorithm was presented to determine the switching points accurately. The presented method can provide solutions with an accuracy to a magnitude of $10^{-12}$ compared with exact solutions obtained by the theories of ordinary differential equations. The PIM results are much more accurate than Newmark ones with the same time step. Moreover, it is found that the PIM can maintain a high level of accuracy even when the time step increases within a relatively wide range.