• Journal of Veterinary Science
• /
• 제21권5호
• /
• pp.79.1-79.12
• /
• 2020

Background: Recently, the pork industry of Thailand faced an epidemic of highly virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV), which spread throughout Southeast Asia, including the Lao People's Democratic Republic and Cambodia. Hence, the rapid and on-site screening of infected pigs on a farm is essential. Objectives: To develop the new aptamer as a biosensor for detection PRRSV which are rapid and on-site screening of infected pig. Methods: New aptamers against PRSSV were identified using the combined techniques of capillary electrophoresis, colorimetric assay by gold nanoparticles, and quartz crystal microbalance (QCM). Results: Thirty-six candidate aptamers of the PRRSV were identified from the systematic evolution of ligands by exponential enrichment (SELEX) by capillary electrophoresis. Only 8 out of 36 aptamers could bind to the PRSSV, as shown in a colorimetric assay. Of the 8 aptamers tested, only the 1F aptamer could bind specifically to the PRSSV when presented with the classical swine fever virus and a pseudo rabies virus. The QCM was used to confirm the specificity and sensitivity of the 1F aptamer with a detection limit of 1.87 × 10¹⁰ particles. Conclusions: SELEX screening of the aptamer equipped with capillary electrophoresis potentially revealed promising candidates for detecting the PRRSV. The 1F aptamer exhibited the highest specificity and selectivity against the PRRSV. These findings suggest that 1F is a promising aptamer for further developing a novel PRRSV rapid detection kit.

• 디지털콘텐츠학회 논문지
• /
• 제18권5호
• /
• pp.905-910
• /
• 2017

본 논문에서는 재난안전통신망의 전체 구조가 아닌 재난현장에서 소방대원과 현장 지휘통제실간의 정보교환이 효과적으로 이루어질 수 있도록 재난현장 지역 내의 독립적 네트워크 인프라(무선통신, 영상전송 및 현장상황파악 등) 확보를 위한 현장형 스마트 통합관제시스템을 제안하였다. 제안한 재난환경 스마트 통합 관제시스템은 재난현장에서 현장대원간의 무선통신을 지원하고, 재난현장 영상정보 수집을 위한 드론과의 통신을 지원함으로써 현장 관제시스템에서 재난상황에 대한 전체 주변 영상확보 및 현장대원의 효율적 지휘가 가능하게 된다.

• Journal of Ecology and Environment
• /
• 제29권4호
• /
• pp.331-335
• /
• 2006

We investigated the distribution of three anuran species, Three-striped pond frogs (Rana nigromaculata), Bullfrogs (Rana catesbeiana), and Narrow-mouthed toads (Kaloula borealis), in an administrative district, Haenam Gun, Junnam Province, Korea using volunteer call surveys. Twenty-eight volunteer call surveyors were assigned to each $2{\times}2km^2$ survey plot. Call surveys on whether the species are present or not were conducted for 5 minutes between 30 minutes after sunset and the midnight on rice fields and ponds from 10 April to 28 August in 2005. Depending on species, call surveys were carried out at seven to 28 plots with average 8.4 to 10.7 visits per the plot. We calculated the detection probabilities and occupancy rates of the three species using four models with three covariates: temperature, humidity, and the amount of water at the habitat. The model average detection probabilities of three anuran species of R. nigromaculata, R. catesbeiana, and K. borealis were 0.53, 0.74, and 0.41 respectively, and the site occupancy rates of them were 0.93, 0.94, and 0.86 respectively. Our results indicate that R. nigromaculata, R. catesbeiana, and K. borealis are common in Haenam Gun.

• Journal of Oral Medicine and Pain
• /
• 제40권3호
• /
• pp.96-101
• /
• 2015

Purpose: Thermal sensory test as an essential part of quantitative sensory testing (QST) has been recognized as a useful tool in the evaluation of the trigeminal nerve function. Normative data in the orofacial region have been reported but the data on differences in the test site, sex and ethnicity are still insufficient. Thus, this study aimed to investigate the normal range of orofacial thermal QST data in the healthy Korean women, and assess sex difference of thermal perception in the orofacial regions. Methods: Thermal QST was conducted on 20 healthy women participants (mean age, 26.4 years; range, 21 to 34 years). The thermal thresholds (cold detection threshold, CDT; warm detection threshold, WDT; cold pain threshold, CPT; and heat pain threshold, HPT) were measured bilaterally at the 5 trigeminal sites (the forehead, cheek, mentum, lower lip and tongue tip). The normative thermal thresholds of women in the orofacial region were evaluated using one-way ANOVA and compared with the previously reported data from age- and site-matched 30 healthy men (mean age, 26.1 years; range, 23 to 32 years) using two-way ANOVA. One experienced operator performed the tests of both sexes and all tests were done in the same condition except the time variability. Results: Women showed significant site differences for the CDT (p<0.001), WDT (p<0.001), and HPT (p=0.047) in the orofacial region. The CDT (p<0.001) and the CPT (p=0.007) presented significant sex difference unlike the WDT and the HPT. Conclusions: The thermal sensory evaluation in the orofacial region should be considered in the context of site and sex and the normative data in this study could be useful for assessment of the sensory abnormalities in the clinical setting.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1999년도 하계학술대회 논문집 B
• /
• pp.677-679
• /
• 1999

This paper introduces a implementation of fault detection for national highway line 3. Fault detection system was installed and operated on national highway line 3, environmental elements caused by abnormal status or faults has often happened. Therefore, the function of fault detection system is to speedy notify fault site, cause as well as scale of fault to manager. Though the fault detection and diagnosis system has been imported in the field of process of water and electric power, it is just beginning step in the field of ITS(Intelligent Transportation Systems). In general, Maintenance system is performed the online/offline process of detection, diagnosis and measure. This paper is studied online detection process, which is realtime remote detection.

• Korean Journal of Radiology
• /
• 제2권4호
• /
• pp.210-215
• /
• 2001

Objective: To investigate the efficacy of gadobenate dimeglumine (GdBOPTA) enhanced MR imaging for the detection of liver lesions in patients with primary malignant hepatic neoplasms. Materials and Methods: Thirty-one patients with histologically proven primary malignancy of the liver were evaluated before and after administration of GdBOPTA at dose 0.05 or 0.10 mmol/kg. T1-weighted spin echo (T1W-SE) and gradient echo (T1W-GRE) images were evaluated for lesion number, location, size and confidence by three off-site independent reviewers and the findings were compared to reference standard imaging (intraoperative ultrasound, computed tomography during arterial portography or lipiodol computed tomography). Results were analyzed for significance using a two-sided McNemar's test. Results: More lesions were identified on Gd-BOPTA enhanced images than on unenhanced images and there was no significant difference in lesion detection between either concentration. The largest benefit was in detection of lesions under 1 cm in size (7 to 21, 9 to 15, 16 to 18 for reviewers A, B, C respectively). In 68% of the patients with more than one lesion, Gd-BOPTA increased the number of lesions detected. Conclusion: Liver MR imaging after Gd-BOPTA increases the detection of liver lesions in patients with primary malignant hepatic neoplasm.

• Bulletin of the Korean Chemical Society
• /
• 제24권1호
• /
• pp.70-74
• /
• 2003

The heterogeneous bioluminescence immunoassay for digoxin was developed using photoprotein, native aequorin as a label and the site-directed immobilization technique based on avidin/biotin interaction. Aequorin is a bioluminescence protein, originally isolated from the jellyfish Aequoria Victoria and an attractive label in analytical applications because of sensitive detection due to virtually no background bioluminescent signal. Digoxin is a cardioactive drug, and its therapeutic level in serum is at low concentration with very narrow therapeutic index. The aequorin-digoxigenin conjugates were synthesized by the N-hydroxysuccinimide ester method and characterized in terms of bioluminescent residual activity. The resulting dose-response curve shows that the detection limit is $1.0\;{\times}\;10^{-10}\;M$ and a dynamic range is three orders of magnitude, which was obtained by $1.0\;{times}\;10^{-10}\;M$ conjugate and 0.9 μg/mL anti-digoxin antibody. Three structurally similar molecules to digoxin were examined for their cross-reactivity. None of these three compounds showed any crossreactivity with digoxin antibody employed in this study. Standard amounts of digoxin corresponding to the therapeutic range were spiked into the each serum solution. Study of the serum matrix effect indicated that correlation coefficient shows good agreement between luminescence light intensity between in buffer and in serum.

• 성인간호학회지
• /
• 제14권2호
• /
• pp.194-204
• /
• 2002

Purpose: This study is aimed at showing the effect of work-site health promotion programs for health promoting behavior, cholesterol, and quality of life of middle-aged workers. Method: Thirty-one middle-aged workers were the experimental group and thirty-one were the control group. The 8-week work-site health promotion program was given to the experimental group. After this, health promoting behavior, cholesterol and quality of life were measured by questionnaires for the experimental and control groups. Health promotion theory, flexibility and muscle strength, aerobic exercise, nutrition, stress management, cancer prevention and early detection, smoking and alcohol problems, and summary lecture were all included in the 8-week work-site health promotion program. Health promoting behavior was measured by Park's HPBS, cholesterol was measured by enzyme method, and quality of life was measured by Ro's QOL. Result: The experimental group showed a higher score of health promoting behavior than the control group. There were no differences on cholesterol and quality of life between the experimental and control groups. Conclusion: It is necessary that nurses provide middle-aged workers with work-site health promotion programs to improve health promoting behavior. It's necessary also to re-study this with the pre-post research design.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제17권6호
• /
• pp.1689-1705
• /
• 2023

To deal with the potential XSS vulnerabilities in the source code of the power communication network, an XSS vulnerability detection method combining the static analysis method with the dynamic testing method is proposed. The static analysis method aims to analyze the structure and content of the source code. We construct a set of feature expressions to match malignant content and set a "variable conversion" method to analyze the data flow of the code that implements interactive functions. The static analysis method explores the vulnerabilities existing in the source code structure and code content. Dynamic testing aims to simulate network attacks to reflect whether there are vulnerabilities in web pages. We construct many attack vectors and implemented the test in the Selenium tool. Due to the combination of the two analysis methods, XSS vulnerability discovery research could be conducted from two aspects: "white-box testing" and "black-box testing". Tests show that this method can effectively detect XSS vulnerabilities in the source code of the power communication network.

• 한국재난정보학회 논문집
• /
• 제18권1호
• /
• pp.154-162
• /
• 2022

연구목적: 건설현장 안전관리를 인적 역량 중심에서 첨단기술인 AIoT와 Mobile 기술을 활용한 시스템 중심의 관리체계로의 전환을 위한 건설현장 안전관리 활성화 방안을 마련하고자 한다. 연구방법: AIoT와 Mobile 기술을 활용한 건설현장 안전관리 모니터링 시스템이 건설현장의 유효성에 대하여 공동주택현장의 골조공종에 종사하고 있는 작업자를 대상으로 가상휀스, 화재감시, 안전모 미착용 인식 3개의 알고리즘을 적용하여 실험을 실시 하였다. 연구결과: 실험대상 근로자는 215명, 가상휀스 침입은 7.61명으로 불안전 행동 발생율은 실험대상 인원 대비 3.5%, 화재감지는 0.16건으로 실험대상 대비 불안전 행동 발생율이 0.07% 나타났으며, 안전모 미착용 인지는 월 평균 안전모 미착용은 8.79명으로 실험대상 대비 불안전 행동 발생율 4.05% 나타났다. 결론: AIoT와 Mobile 기술을 활용한 건설현장 안전관리 모니터링 시스템이 건설현장에 유효한 영향을 미치는 것으로 나타났다.