• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.125-125
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• 2003

Recirculating aquaculture systems consist of different treatment compartments that maintain water quality within the ranges of commonly recommended for fish culture. This paper presents the common considerations in designing different treatment compartments as well as the engineering criteria in designing closed recirculating aquaculture system including a circular tank for fish culture, a sedimentation basin and a foam fractionator for solids removal, two styrofoam bead filters for TAN removal, a sand filter for nitrate removal, and aerators. The main purpose is to outline a common procedure in designing of closed recirculating aquaculture system for marine fish culture.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2017년도 추계학술대회
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• pp.254-255
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• 2017

As the number of ships and goods shipped by marine transportation increases, almost all shipping companies adopted foreign crews in order to overcome lack of seafarers and be competitive for manning cost. Thus, these days it is inevitable to work with foreign crews aboard ships, but there occur many marine accidents due to language barriers and communication problems under multicultural and multilingual shipboard environments. In order to resolve troubles resulting from different languages, reduce miscommunication risks, and make working on ships safe and efficient, this study recognized the current status of ships with mixed crews, analyzed marine accidents caused by communication problems not only in Korea but also in other countries using analytical methodologies. Additionally, existing hazards affecting miscommunication were identified and risk of miscommunication was quantitatively evaluated while offering suggestions and future forecasts. Consequently, in this study we suggested that fundamental dialogues are to be necessarily educated on the ships with foreign crews so as to reduce the language barrier and risk of miscommunication. Furthermore, a standard maritime Korean language program and manual targeting Korean coastal vessels would be developed in the next step.

• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1420-1427
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• 2016

Geosmin and 2-methylisoborneol (2-MIB) are two of the most common taint compounds that adversely affect the quality of aquacultural animals. In the present study, 94% of geosmin and 97% of 2-MIB in suspended growth reactors producing bioflocs (SGRs) with aquaculture waste were removed after inoculation with Bacillus subtilis, significantly higher than that of control SGRs (70% of geosmin and 86.4% of 2-MIB). The lowest concentrations of geosmin and 2-MIB achieved in the effluent of the SGRs were 2.43 ± 0.42 ng/l and 2.23 ± 0.15 ng/l, respectively. The crude protein content of the bioflocs produced in the SGRs was 35 ± 4%. The NH₄⁺-N and NO₂^--N concentrations in the effluent of the reactors were 1.13 ± 0.21 mg/l and 0.42 ± 0.04 mg/l, respectively. These results suggest that inoculated with Bacillus subtilis, SGRs have a better performance to reuse the nitrogen in fish waste and to remove geosmin and 2-MIB from the culture water efficiently.

• 한국발생생물학회지:발생과생식
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• 제12권1호
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• pp.51-56
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• 2008

제브라피쉬 배아 유래세포의 최적 성장조건을 확립하기 위해 3종류의 배지 DMEM, K-NAC, D-NAC 그룹에서의 세포 성장률을 비교하였다. 실험 결과, 접종밀도에 따른 성장률의 경우, DMEM에 비해 K-NAC 그룹에서 초기 접종 효율이 높게 나타났으며, 후기 성장률은 DMEM 그룹에서 높게 나타났다. K-NAC, DMEM 그룹 모두 FBS 농도에 의한 성장차는 보이지 않았으며, 0.1% embryo extract를 첨가한 배지에서 효과는 낮게 나타났으나 1% trout serum 첨가한 경우 매우 높은 성장률을 보였다(p<0.05). $2-3{\times}10^5$ 밀도로 접종한 그룹에서는 유의차가 없었으나, $4{\sim}5{\times}10^5$ 밀도에서는 DMEM 그룹이 K-NAC 그룹보다 다소 높은 성장률을 보였다(p<0.1). DMEM과 D-NAC 그룹에서의 FBS 농도에 따른 성장률을 비교한 결과, FBS 농도에 따른 성장차는 유의하지 않았으나(p<0.05), D-NAC의 모든 실험군이 DMEM 그룹에 비해 높은 성장률을 보였다(p<0.05).

• Ocean and Polar Research
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• 제25권4호
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• pp.493-501
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• 2003

Performance of a laboratory scale closed seawater recirculating aquaculture system was evaluated. Twenty-kg of korean rockfish (130 fish) with an average body weight of 153.8g was stocked. Over a 107-day culture period, fish reached final density of $51.7kg/m^3$ (initial density, $33.3kg/m^3$) on the basis of the culture tank volume. On a daily basis, added water amounted to 3.4% of the total water volume in the system. Total ammonia nitrogen (TAN) concentrations were below 1mg/l and nitrite nitrogen $(NO_2-N)$ concentrations were within the range of 1-3mg/l on most sampling days. TAN was removed from bead and sand filters and it was removed or produced in the sedimentation basin. Basically, $NO_2-N$ was removed in the bead and sand filters, while it was either removed or produced in the sedimentation basin. Nitrate nitrogen $(NO_3-N)$ was produced in the bead filters and removed from the sand filter and sedimentation basin. The foam fractionator performed well in the recirculating system. The maximal daily removal values for total suspended solids (755) and protein were 10.9g and 1.4g, respectively. Whole water quality parameters were within the levels commonly recommended for fish culture on most of the sampling days. However, further studies are needed to evaluate the commercial feasibility of this system because of the smallscale system used in present experiment. At least, the present study still provides some basic information for further studies of this kind of system.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1403-1406
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• 2007

An antiproliferative agent, streptochlorin, was isolated from the fermentation broth of a marine actinomycete isolated from marine sediment. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Streptomyces. Bioactivity guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and reversed-phase HPLC gave a pure compound, streptochlorin. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Streptochlorin exhibited significant antiproliferative activity against human cultured cell lines.

• 한국항만경제학회지
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• 제26권4호
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• pp.269-288
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• 2010

21세기를 문화의 시대라 하며, 인간의 삶의 모든 현장에서 문화가 중요시되고 있다. 모든 활동에서 문화중심적인 사고와 문화를 통한 그리고 문화의 정립을 위한 활동과 전략들이 중요시 되고 있다. 바다는 인간의 미지의 세계에 대한 동경과 동시에 도전의 이미지로 다가오며, 항만은 인간이 살고 있는 육지와 동경의 바다를 연결하는 연결점으로 이해된다. 바다와 섬과 항만은 인간의 삶의 터전이며 경제활동이며 자연과 소통하는 공간이다. 삶과 일과 놀이와 상상의 공간으로서 바다와 항만은 인류의 역사와 함께 발전하고 변모해 왔다. 해양활동은 시간과 지역에 따라 여러 모습으로 다양성을 담지하는 문화의 형태로 발전되게 된다. 본 연구에서는 해양문화에 대한 개념정립과 이론적 검토 그리고 도입 및 확장을 위한 정책적이고 전략적인 대안이 모색되었다. 해양문화는 주민의 삶의 질을 높이며 동시에 경제적 가치로서 문화, 관광, 환경의 질을 제고하여 국민 경제에 기여함이 크고 향후 기대가 커지고 있다. 본 연구에서는 제안된 이론적 틀과 이을 통한 체계적인 해양문화의 도입과 확장이 요청되며, 세부 전략과 대안에 대한 심도있는 연구가 추가적으로 실행될 필요가 크다고 본다.

• KSBB Journal
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• 제28권4호
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• pp.217-224
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• 2013

A bacterium CGH18 exhibiting antioxidizing and chitin-degrading activities in the colloidal chitin culture medium was isolated from salt-fermented crab. This strain was identified as Bacillus idriensis based on 16S rDNA sequence homology search. Its crude extract was partitioned between n-BuOH and $H_2O$. The organic layer was further partitioned between $CH_2$ $Cl_2$ and $H_2O$. Antioxidant activities of crude extract and its solvent fractions were evaluated using five different bioassay methods, including the degree of occurrence of intracellular reactive oxygen species (ROS), peroxynitrite scavenging (ONOO), and oxidative damage of genomic DNA. All fractions exhibited significant antioxidant activity in bioassay systems used.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1559-1565
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• 2014

Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a $200{\mu}l$ total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at $100^{\circ}C$ for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
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• pp.266-266
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• 2002