Browse > Article
http://dx.doi.org/10.4014/jmb.1603.03043

Effects of Inoculated Bacillus subtilis on Geosmin and 2-Methylisoborneol Removal in Suspended Growth Reactors Using Aquacultural Waste for Biofloc Production  

Luo, Guozhi (College of Fisheries and Life Sciences of Shanghai Ocean University)
Wang, Jiao (College of Fisheries and Life Sciences of Shanghai Ocean University)
Ma, Niannian (College of Fisheries and Life Sciences of Shanghai Ocean University)
Liu, Zefeng (College of Fisheries and Life Sciences of Shanghai Ocean University)
Tan, Hongxin (College of Fisheries and Life Sciences of Shanghai Ocean University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.8, 2016 , pp. 1420-1427 More about this Journal
Abstract
Geosmin and 2-methylisoborneol (2-MIB) are two of the most common taint compounds that adversely affect the quality of aquacultural animals. In the present study, 94% of geosmin and 97% of 2-MIB in suspended growth reactors producing bioflocs (SGRs) with aquaculture waste were removed after inoculation with Bacillus subtilis, significantly higher than that of control SGRs (70% of geosmin and 86.4% of 2-MIB). The lowest concentrations of geosmin and 2-MIB achieved in the effluent of the SGRs were 2.43 ± 0.42 ng/l and 2.23 ± 0.15 ng/l, respectively. The crude protein content of the bioflocs produced in the SGRs was 35 ± 4%. The NH4+-N and NO2--N concentrations in the effluent of the reactors were 1.13 ± 0.21 mg/l and 0.42 ± 0.04 mg/l, respectively. These results suggest that inoculated with Bacillus subtilis, SGRs have a better performance to reuse the nitrogen in fish waste and to remove geosmin and 2-MIB from the culture water efficiently.
Keywords
Geosmin; 2-methylisoborneol; biofloc technology; Bacillus subtilis; aquaculture waste;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Davidson J, Schrader K, Ruan E, Swift B, Aalhus J, Juarez M, et al. 2014. Evaluation of depuration procedures to mitigate the off-flavor compounds geosmin and 2-methylisoborneol from Atlantic salmon Salmo salar raised to market-size in recirculating aquaculture systems. Aquac. Eng. 61: 27-34.   DOI
2 Azim ME, Little DC. 2008. The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture 283: 29-35.   DOI
3 Burr GS, Wolters WR, Schrader KK, Summerfelt ST. 2012. Impact of depuration of earthy-musty off-flavors on fillet quality of Atlantic salmon, Salmo salar, cultured in a recirculating aquaculture system. Aquac. Eng. 50: 28-36.   DOI
4 Chu C, Lee D. 2004. Multiscale structures of biological flocs. Chem. Eng. Sci. 59: 1875-1883.   DOI
5 de Schryver P, Boon N, Verstraete W, Bossier P. 2012. The biology and biotechnology behind bioflocs, pp. 199-215. In Avnimelech Y (ed.). Biofloc Technology: A Practical Guide Book, 2nd Ed. The World Aquaculture Society, Baton Rouge, LA, USA.
6 Ebeling JM, Timmons MB, Bisogni JJ. 2006. Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia-nitrogen in aquaculture systems. Aquaculture 257: 346-358.   DOI
7 Egashira K, Ito K, Yoshiy Y. 1992. Removal of musty odor compound in drinking water by biological filter. Water Sci. Technol. 25: 307-314.
8 Guttman L, van Rijn J. 2009. 2-Methylisoborneol and geosmin uptake by organic sludge derived from a recirculating aquaculture system. Water Res. 43: 474-480.   DOI
9 Guttman L, van Rijn J. 2012. Isolation of bacteria capable of growth with 2-methylisoborneol and geosmin as the sole carbon and energy sources. Appl. Environ. Microbiol. 78: 363-370.   DOI
10 Ho L, Tang T, Monis PT, Hoefel D. 2012. Biodegradation of multiple cyanobacterial metabolites in drinking water supplies. Chemosphere 87: 1149-1154.   DOI
11 Hargreaves JA. 2006. Photosynthetic suspended-growth systems in aquaculture. Aquac. Eng. 34: 344-363.   DOI
12 Hargreaves JA. 2013. Bioflocs production system for aquaculture. Southern Regional Aquaculture Center (SRAC) Publication No. 4503.
13 Ho L, Hoefel D, Bock F, Saint CP, Newcombe G. 2007. Biodegradation rates of 2-methylisoborneol (2-MIB) and geosmin through sand filters and in bioreactors. Chemosphere 66: 2210-2218.   DOI
14 Izaguirre G, Wolfe RL, Means EG. 1988. Degradation of 2-methylisoborneol by aquatic bacteria. Appl. Environ. Microbiol. 54: 2424-2431.
15 Kuhn DD, Boardman GD, Lawrence AL, Marsh L, Flick Jr GJ. 2009. Microbial floc meal as a replacement ingredient for fish meal and soybean protein in shrimp feed. Aquaculture 296: 51-57.   DOI
16 Lauderdale CV, Aldrich HC, Lindner AS. 2004. Isolation and characterization of a bacterium capable of removing taste- and odor-causing 2-methylisoborneol from water. Water Res. 38: 4135-4142.   DOI
17 Lloyd SW, Lea JM, Zimba PV, Grimm CC. 1998. Rapid analysis of geosmin and 2-methylisoborneol in water using solid phase micro extraction procedures. Water Res. 32: 2140-2146.   DOI
18 Nam Koong H, Schroederb JP, Petrickc G, Schulz C. 2016. Removal of the off-flavor compounds geosmin and 2-methylisoborneol from recirculating aquaculture system water by ultrasonically induced cavitation. Aquac. Eng. 70: 73-80.   DOI
19 Lu L, Tan HX, Luo GZ, Liang WY. 2012. The effects of Bacillus subtilis on nitrogen recycling from aquaculture solid waste using heterotrophic nitrogen assimilation in sequencing batch reactors. Bioresour. Technol. 124: 180-185.   DOI
20 Rittmann BE, Gantzer CJ, Montiel A. 1995. Biological treatment to control taste-and-odor compounds in drinking water treatment, pp. 209-246. In Suffet IH, Mallevialle J, Kawczynski E (eds.). Advances in Taste-and-Odor Treatment and Control. American Water Works Association Research Foundation, Denver, USA.
21 Ng C, Losso JN, Marshall WE, Rao RM. 2002. Freundlich adsorption isotherms of agricultural by-product-based powdered activated carbons in a geosmin–water system. Bioresour. Technol. 85: 131-135.   DOI
22 Rangesh S, George AS. 2011. Treatment of taste and odor causing compounds 2-methyl isoborneol and geosmin in drinking water: a critical review. J. Environ. Sci. 23: 1-13.   DOI
23 Saito A, Tokuyama T, Tanaka A, Oritani T, Fuchigami K, 1999. Microbiological degradation of (-)-geosmin. Water Res. 33: 3033-3036.   DOI
24 Schneider O, Sereti V, Eding EH, Verreth JA. 2007. Heterotrophic bacterial production on solid fish waste: TAN and nitrate as nitrogen source under practical RAS conditions. Bioresour. Technol. 98: 1924-1930.   DOI
25 Schrader KK, Rubio SA, Piedrahita RH, Rimando AM. 2005. Geosmin and 2-methylisoborneol cause off-flavors in cultured largemouth bass and white sturgeon reared in recirculatingwater systems. N. Am. J. Aquac. 67: 177-180.   DOI
26 Watson SB, Brownlee B, Satchwill T, Hargesheimer EE. 2000. Quantitative analysis of trace levels of geosmin and 2-MIB in source and drinking water using headspace SPME. Water Res. 34: 2818-2828.   DOI
27 Schryver PD, Verstraete W. 2009. Nitrogen removal from aquaculture pond water by heterotrophic nitrogen assimilation in lab-scale sequencing batch reactors. Bioresour. Technol. 100: 1162-1167.   DOI
28 Tucker CS. 2000. Off-flavor problems in aquaculture. Rev. Fish Sci. 8: 45-88.   DOI
29 Yang L, Chou LS, Shieh WK. 2001. Biofilter treatment of aquaculture water for reuse application. Water Res. 35: 3097-3108.   DOI
30 Wu Y, Li T, Yang L. 2012. Mechanisms of removing pollutants from aqueous solutions by microorganisms and their aggregates: a review. Bioresour. Technol. 107: 10-18.   DOI
31 Yagi M, Nakashima S, Muramoto S. 1988. Biological degradation of musty odor compounds, 2-methylisoborneol and geosmin, in a bio-activated carbon filter. Water Sci. Technol. 20: 255-260.
32 Zamyadi A, Henderson R, Stuetz R, Hofmann R, Ho L. 2015. Fate of geosmin and 2-methylisoborneol in full-scale water treatment plants. Water Res. 83: 171-183.   DOI
33 Zoschke K, Dietrich N, Bornick H, Worch E. 2012. UV-based advanced oxidation processes for the treatment of odour compounds: efficiency and by-product formation. Water Res. 46: 5365-5373.   DOI
34 Avnimelech Y. 1999. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture 176: 227-235.   DOI
35 APHA (American Public Health Association). 2005. Standard Methods for Examinations of Water and Wastewater, 21st Ed. American Water Work s Association, and Water Environment Federation, Washington, DC.