• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.72-72
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• 2003

The present study examined the possibility of cryopreservation of the D-shaped and umbo larvae of arkshell (Scapharca broughtonii), in terms of the survival rates after freezing and thawing. D-shaped and umbo larvae of arkshells were obtained from a shellfish farming on Yosu city. The average shell lengths were $93.3 \pm 10.1 \mu$m and $201.7 \pm 13.5 \mu$, respectively. Five cryoprotectants (CPAs), dimethyl sulfoxide (DMSO), glycerol, ethylene glycol (EG), propylene glycol (PG), and methanol, were tested at the concentrations of 1.5, 2.0 and 2.5 M. After larvae suspended in CPAs, cryoprotectants were loaded in 0.5 ml straws at a larval density of 50-100 larvae per straw, and epuilibrated for 10 and 20 minute at room temperature ($23^{\circ}C$), repectively. Straws were cooled at a rate of $1^{\circ}C$/min from $0^{\circ}C$ to $-12^{\circ}C$, held for 5 min at $-12^{\circ}C$, and then cooled at $2^{\circ}C$/min to $-35^{\circ}C$ and equilibrated for 5 min followed by plunging in liquid nitrogen. After storage in liquid nitrogen for 1 day, straws were thawed in a $30^{\circ}C$ water. As soon as straws were observed to melt, larvae were diluted with an equal volume of ASW and then washed twice with a large volume of ASW at an interval of 2 min to unload the CPAs. The results showed that after equilibration for 10 and 20 minute at room temperature, no larvae survived using methanol as CPAs, and it was observed that larval shells all open slightly, and larval flesh broke down and slopped over the shells. The highest survival rates (D-shaped larvae: 77.6%, umbo larvae: 59.3%) were obtained with 2M DMSO, and 1.5M glycerol yielded survival rates of 53.8% for D-shaped larvae and 37.5% for umbo larvae. The surviving D-shaped larvae showed active rotary motion and perfect membrane integrity and cytoplasmic normality, and the vigorous movement of veliger cilia was observed inside the closed shells. The breakdown of tissue occurred in the abnormal larvae, and the isolated cell often run out of shells.

• Journal of Korean Port Research
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• v.11 no.2
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• pp.321-337
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• 1997

Rapid change in the technological environment of marine transportation and the development of the ocean shipping industry have fostered a revolution in the port system. This in turn has caused major changes in the function and use of port in Korea. Aside from this. Mokpo Port, however continues to decline, because the existing port facilities and related subsystem are already obsolete with no chance of regaining operational effectiveness and treatment for proper implementation. Although a few studies have been done on the Mokpo Port, has not been found, any reseach for the analytical approach to the transportation system of it. This paper aims to make an extensive analysis of the physical distribution system in Mokpo Port focusing on the coordination of subsystems such as navigational aids system, quay handling and transfer system, storage system and inland transport system. The base of introduced simulation tool here is the queueing theory. The overall findings are as follows; 1. Among those vessels called at Mokpo Port in 1994, the average size of oceangoing vessels is 4,922.1 G/T, and the domestic is 317.8 G/T. The average arrival interval and service time of the domestic vessels are 6.0 hours and 24.1 hours respectively marking the berth occupation rate over 100%. Those for oceangoing vessels are 34.5 hours, 120.0 hours and 37.2%. In order to maintainin the berth occupation rate to 70% the capacity considering the 1994 of domestic piers must be extended to 145% and oceangoing vessels must be increased to 165% year called. 2. The capacity of approaching channel is enough to handle the total traffic volume. 3. Tugs are sufficiently being provided to handle all ships requiring their services 4. The capacity of storage and inland transportation systems are sufficient to handle the throughput and the yard stroage utilization rate of No.1 $\cdots$ No.5 is 4.5% and No.6 1S 30% of 1993's. 5. The utilization rate of LLc(Level Looping Crane) and PNT(PNeumaTic) are 2.7% and 18.8%, respectively. Practical solution and proposal for improvement of Transportation System in Mokpo Port are as follows; 1. To avoid the congestion in domestic pier introduction of a new port operation system is necessary allowing the domestic vessel to use the oceangoing pier. 2. To establish the port management information system to improve the efficiency of port operation. 3. To build a new storage system for high valued cargos including modernization of the present storage and handling system. 4. To insure the safety of navigation in approaching channel, The Vessel Traffic System including separation scheme is introduced. 5. To interest enormously on public relation to ship owner's association, shippers and consignees by showing that they can save cost and ship turnaround time in order to promote the call to Mokpo Port. At last, to be strategically change the function of Mokpo Port to the Leisure, Fishing & Ferry as well as Maritime port.

• Environmental and Resource Economics Review
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• v.31 no.4
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• pp.777-799
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• 2022

This study examined the latest EU regulatory strengthening trends for refrigerant gases with very large global warming potential (GWP) and derived implications for carbon neutrality. The European Commission recently unveiled an amendment that significantly strengthens the F-gas Regulation. This study presented the meaning of the main contents related to refrigerants in the amendment by comparing them with the current regulations. The main contents of the amendment include drastically reducing the maximum amount of HFCs that can be placed on the market, strengthening regulations related to HFCs allocation, adding products and equipment that use high GWP refrigerants, adding regulated F-gas and updating the GWP of existing gases, and other stricter regulatory designs. This movement of the EU will affect the policy stance of advanced countries such as the United States and Japan, and Korea's policy will also be further strengthened. Therefore, it will be inevitable for related industries to change to next-generation refrigerant gas. Meanwhile, this study also analyzed the latest policy trends related to per- and polyfluoralkyl substances (PFAS) regulation, which were not noted in previsou studies on refrigerants and F-gas. If PFAS's registration of REACH restricted substances, which are being promoted by five European countries, is made, it will have a very big impact on the industry regarding refrigerant gas. In addition, it will be inevitable to thoroughly review each country's greenhouse gas reduction strategies related to F-gas materials, including refrigerants.

• Korean Journal of Ecology and Environment
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• v.56 no.1
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• pp.70-82
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• 2023

Recently, Korean government has introduced Multi Metric Indices (MMI) using various biocommunity information for aquatic ecosystem monitoring and ecosystem health assessment at the national level. MMI is a key tool in national ecosystem health assessment programs. The MMI consists of indices that respond to different target environmental factors, including environmental disturbance (e.g. nutrients, hydrological and hydraulic situation of site etc.). We used zooplankton community information collected from Korean lakes to estimate the availability of candidate zooplankton MMI indices that can be used to assess lake ecosystem health. First, we modified the candidate indices proposed by the U.S. EPA to suit Korean conditions. The modified indices were subjected to individual index suitability analysis, correlation analysis with environmental variables, and redundancy analysis among indices, and 19 indices were finally selected. Taxonomic diversity was suggested to be an important indicator for all three taxonomic groups (cladoceran, copepod, rotifer), on the other hand, the indices using biomass for large cladocerans and copepods, while the indices using abundance were suggested for small cladocerans and rotifers.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.1
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• pp.1-7
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• 2023

An artificial intelligence (AI) method based on image deep learning is proposed to predict the entire displacement shape of a structure using the feature of partial displacements. The performance of the method was investigated through a structural test of a steel frame. An image-to-image regression (I2IR) training method was developed based on the U-Net layer for image recognition. In the I2IR method, the U-Net is modified to generate images of entire displacement shapes when images of partial displacement shapes of structures are input to the AI network. Furthermore, the training of displacements combined with the location feature was developed so that nodal displacement values with corresponding nodal coordinates could be used in AI training. The proposed training methods can consider correlations between nodal displacements in 3D space, and the accuracy of displacement predictions is improved compared with artificial neural network training methods. Displacements of the steel frame were predicted during the structural tests using the proposed methods and compared with 3D scanning data of displacement shapes. The results show that the proposed AI prediction properly follows the measured displacements using 3D scanning.

• Journal of Navigation and Port Research
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• v.47 no.1
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• pp.18-24
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• 2023

After the Great East Japan Earthquake, many reports and books that compiled testimonies of adult victims were published. Thus, refugee situations are well known, but information on the refugee situations of Japanese students is not. This is because what actions the students took and how they sought refuge from an earthquake or tsunami have not been fully recognized. The purpose of this study was to examine and analyze students' refuge behavior in the Great East Japan Earthquake and to predict the refuge behavior of students affected by future disasters. The results of the study showed that students passively acquired information about earthquakes and tsunamis and that their refuge behavior was highly dependent on adults. Immediately after an earthquake, people tended to protect themselves and stay in place until the shaking stopped. However, they tended to move to another place after the shaking occurred frequently. Students living on ria coastlines were likely to move to high places to escape the threat of earthquakes and tsunamis, whereas students living in plain regions were likely to move vertically to tall buildings, such as schools. As for the mode of movement to refugee shelters, the students arrived at the final refugee shelters in one move, and it is assumed that the refugee shelters should be decided in advance and the students should move there.

• Journal of the Korean Geosynthetics Society
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• v.22 no.1
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• pp.87-96
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• 2023

There are a lot of difference between the surface settlement and the differential settlement measured at the Busan New Port, where the dredged and reclaimed clay layer exists and below the clay is originally thickly distributed. To find the cause and solution of this, the actual conditions of each differential settlement used for the soft ground improvement, characteristics, installation method, measurement frequency, measurement data management, and data analysis of each type were considered. In the deep soft ground improvement work where large deformation occurs, the bending deformation of the screw-type differential settlement gauge is less than that of other types of measuring instruments, so there is less risk of loss, and the reliability of data is relatively high as the instruments are installed by drilling for each stratum. Since the greater the amount of high-precision settlement measurement data, the higher the settlement analysis precision. It is necessary to manage with higher criteria than the measurement frequency suggested in the standard specification. For the data management of the differential settlement gauge, it is desirable to create graphs of the settlement and embankment height of the relevant section over time, such as surface, differential, and settlement of pore water pressure gauge for each point. In the case of multi-layered ground with different compression characteristics, it is more appropriate to perform settlement analysis by calculating the consolidation characteristics of each stratum using a differential settlement data.

• Journal of the Korean Geotechnical Society
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• v.26 no.3
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• pp.13-23
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• 2010

In this study, centrifuge model tests with 50 g gravitational condition were performed to evaluate the bearing capacity of very soft ground, improved by spreading geotextile and sand on the surface of ground, for the heavy machinery to be able to access. For undrained shear strength of ground model, prepared with the clay sampled from the field, being in the range of 3.1~11.7 kPa, bearing capacity tests were performed with the model footing and the loading system built to simulate the heavy machinery on the ground model treated with geotextile and sand. Test results were compared with theoretically and numerically evaluated ones. Test results about load-settlement curves showed that the bearing capacity increases with the increase of the undrained shear strength of ground. Punching shear or local shear failure was also observed. For a relatively low undrained shear strength of ground, settlement behavior is found to be crucial to evaluating the trafficability of machinery whereas bearing capacity becomes a dominant factor with the increase of undrained shear strength of ground. The method for assessing the bearing capacity of the ground related to trafficability of machinery is presented by acquiring the regression relationship between the contact pressure of machinery and settlements using load-settlement curves with the change of the undrained shear strength. Furthermore, results of numerical analyses about load-settlement relation are in relatively good agreement with those of centrifuge model test.

• Journal of the Korean Institute of Rural Architecture
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• v.25 no.2
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• pp.1-8
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• 2023

This study reveals the absence of standard design guidelines by region through investigation and analysis centered on public design cases by region in Jeollabuk-do and design elements that can reflect the integration, identity, and diversity of public design in each region by five regions. Through this, the following conclusions could be obtained. First, to improve the quality of street facilities in Jeollabuk-do, the design elements (design motif, color, pattern) applicable to the standard design were analyzed by dividing them into five regions. As a design motif, it was possible to extract patterns containing straight lines, sophistication, dignity, and smartness. In the Northeast region, it is comfortable with the motif of the mountain ridge reflecting geographical characteristics, and it can be extracted elements that contain warm and natural colors. In the southeastern region, patterns that reflect design elements were extracted by applying safe, lively, and peaceful colors with the design motif of curves that blend nature and agriculture. In the southwestern region, design pattern elements that highlight nature, history, and culture were extracted with various cultural assets and natural greenery as motifs. Lastly, in the Saemangeum region, the ocean flow and greenery could be used as a design motif to reflect a positive, clear, future-oriented image in the design spot zones by region. Second, based on the standard design elements (design motive, color, pattern) by region extracted for the standard design development of street facilities in each region in Jeollabuk-do, an integrated zone(Form, structure, material, color, functional element) to which regional design guidelines can be applied. Third, an integrated zone (form, structure, material, color, functional elements) was composed. In addition, design spot zones (patterns and colors in city and county units) that can contain the diversity and identity of each region were designated. By designating design spot zones (patterns and colors in city and county units) that can contain the diversity and identity of each region, standard design development plans (integrated pillars, jaywalking prevention fences, roundabouts (urban type, rural type), street trees) Eight standard designs, including protective covers, street planters, flat benches, light benches, visual media for user guidance, and parking zones for personal mobile devices) were presented.

• Journal of the Korea Society of Computer and Information
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• v.28 no.5
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• pp.163-170
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• 2023

As the development of the 4th industrial revolution in the maritime industry has accelerated, the technical development and progress of maritime autonomous surface ship(MASS), and the development of international regulations have been accelerated. In particular, the IMO Maritime Safety Committee(MSC) has established a road-map for the development of the non-mandatory goal-based MASS instrument(MASS Code) and started developing a non-mandatory MASS Code at MSC 105th meeting. Many countries are actively participating in the Correspondence Group on the development of MASS Code, and the development of detailed requirements for MASS functions in the MASS Code is underway. Especially, the concept of "Mode of Operation" for MASS functions was mentioned in the Correspondence Group for the first time, and it is expected that discussions on these modes will be conducted from the IMO MASS JWG meeting to held in April 2023. The concept of "Mode of Operation" will be useful in explaining MASS and MASS functions and will be discussed continually for the development of MASS Code. This paper reviews the contents of the IMCA M 220 document, which provides guidelines on operating modes, to conduct research on the benchmark for setting the operating modes of MASS.