• Journal of Korean Society of Environmental Engineers
• /
• v.39 no.5
• /
• pp.288-302
• /
• 2017

Accurate measurement of benthic nutrient fluxes (BNF) is a prerequisite for evaluating the effect of sediments on nutrient cycle in the surface water. The intact sediment cores were collected in July 2015 at the midstream of Nakdong River. We identified pre-incubation time (6, 12, 24 hr), dissolved oxygen concentration (90, 70, 50% saturation), diffusive boundary layer thickness (0, 0.6-0.8, 1.2-1.4 mm), and incubation temperature (10, 17, 20, $25^{\circ}C$) as the most important control factors, and measured the BNF fluctuation with the variation of these factors using the laboratory sediment core incubation method. Since the chemical composition, redox condition, hydrodynamic regimes and microbial activities at the sediment-water interface were changed as a result of the alteration of control factors, sediment core incubation should be conducted under as close to the natural conditions of study site as possible, in order to produce the results similar to actual values. Relative percentage differences between two replicates were below 20% in most control factors, which showed satisfactory precision for strict compliance with the experimental conditions and procedures. In the further studies, we will compare the results of core incubation with those of in situ measurements to confirm the accuracy of the sediment core incubation method.

• Biomolecules & Therapeutics
• /
• v.26 no.1
• /
• pp.10-18
• /
• 2018

Tumors are dynamic metabolic systems which highly augmented metabolic fluxes and nutrient needs to support cellular proliferation and physiological function. For many years, a central hallmark of tumor metabolism has emphasized a uniformly elevated aerobic glycolysis as a critical feature of tumorigenecity. This led to extensive efforts of targeting glycolysis in human cancers. However, clinical attempts to target glycolysis and glucose metabolism have proven to be challenging. Recent advancements revealing a high degree of metabolic heterogeneity and plasticity embedded among various human cancers may paint a new picture of metabolic targeting for cancer therapies with a renewed interest in glucose metabolism. In this review, we will discuss diverse oncogenic and molecular alterations that drive distinct and heterogeneous glucose metabolism in cancers. We will also discuss a new perspective on how aberrantly altered glycolysis in response to oncogenic signaling is further influenced and remodeled by dynamic metabolic interaction with surrounding tumor-associated stromal cells.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.22 no.6
• /
• pp.708-722
• /
• 2016

Land-Ocean Interactions in the Coastal Zone (LOICZ) models for nutrient budgets were used to estimate the seasonal capacity of the Youngsan Estuary and Youngam-Geumho Estuary to sink and/or supply nutrients such as dissolved inorganic phosphorus (DIP) and nitrogen (DIN) to provide an understanding of the behavior of the coupled biogeochemical cycles of phosphorus and nitrogen in the estuaries (Youngsan Estuary, Youngam-Geumho Estuary) near Mokpo Harbor. During non-stratified periods (May, September, and November, 2008), simple three-box models were applied in each sub-region of the system, while a two-layer box model was applied during on-site observation of stratification development (July, 2008). The resulting mass-balance calculation indicated that even after large discharges from artificial lakes (in May and July), DIP influxes due to a mixing exchange ($V_{X-3}$, or $V_{deep}$) were more than terrigenous loads, indicating the backward transportation of nutrients from a marine source. The model results also indicated that for nutrient loads (DIP and DIN fluxes) in September, an extreme congestion of nutrients occurred around the mouths (sub-region III of the model) of the estuaries, possibly due to an imbalance in physical circulations between the estuaries and offshore locations. In November, the Youngam-Geumho Estuary, into which freshwater was discharged from artificial lakes (Youngam and Geumho Lake), showed nutrient enrichment in the water column, but the Youngsan Estuary showed nutrient depletion. In conclusion, to efficiently control water quality in the estuaries near Mokpo Harbor, integrated environmental management programs should be implemented. I.e., the reduction of nutrient loads from land basins as well as the deposit of nutrient loads into adjacent coastal lines.

• Korean Journal of Ecology and Environment
• /
• v.36 no.3 s.104
• /
• pp.311-321
• /
• 2003

We surveyed physico-chemical properties of bottom water and sediment to evaluate the influence of sediment on the eutrophication in the downstream of Nagdong River from 1998 to 2000. From May to August, DO concentration of bottom waters dropped below 5 mg $O_2/l$ and $NH_4\;^+$ and $PO_4\;^{3-}$ concentrations significantly increased in the bottom waters, resulting in the great differences between surface and bottom waters. Fluxes across water-sediment interface would be substantially active in this period. The serial orders of the water fertility or eutrophication were Joman River > Sonagdong River > Nagdong River. The organic nutrient contents of sediment increased toward the lower parts of the river system. Organic contents of the sediment would be under the influence of water pollution and exhibited a negative correlation with sediment bulk-density or particle size. The concentrations of exchangeable inorganic nutrients of sediment were greater than those of pore waters, and $PO_4\;^{3-}$, $NH_4\;^+$ and $SiO_2$ increased along the sediment depth. $PO_4\;^{3-}$ and $NO_3\;^-$ concentrations of the pore water were less than the overlying waters, while NH4+ and $SiO_2$ concentrations showed opposite trends. Exchangeable nutrients of sediment could be the repository for t]me nutrient exchange in the water-sediment interface.

• Journal of Environmental Science International
• /
• v.28 no.10
• /
• pp.813-829
• /
• 2019

We measured the amount of precipitation, stemflow, and throughfall and concentrations of nine major inorganic nutrients ($H^+$, ${NH_4}^+$, $Ca^{2+}$, $Mg^{2+}$, $K^+$, $Na^+$, $Cl^-$, ${NO_3}^-$, and ${SO_4}^{2-}$) to investigate the nutrient inputs into soil from precipitation in Pinus densiflora and Quercus mongolica stands from September 2015 to August 2016. The precipitation inputs of $H^+$, ${NH_4}^+$, $Ca^{2+}$, $Mg^{2+}$, $K^+$, $Na^+$, $Cl^-$, ${NO_3}^-$, and ${SO_4}^{2-}$ into soil were 0.170, 15.124, 42.227, 19.218, 14.050, 15.887, 22.391, 5.431, and $129.440kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, respectively. The P. densiflora stemflow inputs were 0.008, 0.784, 1.652, 1.044, 0.476, 0.651, 1.509, 0.278, and $9.098kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, and those for Q. mongolica were 0.008, 0.684, 2.429, 2.417, 2.941, 1.398, 2.407, 0.436, and $13.504kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, respectively. The P. densiflora throughfall inputs were 0.042, 21.518, 52.207 27.694, 20.060, 24.049, 37.229, 10.241, and $153.790kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, and those for Q. mongolica were 0.032, 15.068, 42.834, 21.219, 20.294, 20.237, 24.288, 5.647, and $119.134kg{\cdot}ha^{-1}{\cdot}yr^{-1}$, respectively. Of the total throughfall flux (i.e., stemflow + throughfall flux) of the nine ions for the two species, ${SO_4}^{2-}$ had the greatest total throughfall flux and $H^+$ had the lowest. The net throughfall fluxes of the ions for the two species had various correlations with the precedent dry period, rainfall intensity, rainfall amount, and pH of precipitation. The soil pH under the Q. mongolica canopy (4.88) was higher than that under the P. densiflora canopy (4.34). The difference in the soil pH between the two stands was significant (P < 0.01), but the difference in soil pH by the distance from the stems of the two species was not (P > 0.01). This study shows the enrichments of inorganic nutrients by two representative urban forests in temperate regions and the roles of urban forests during rainfall events in a year.

• Korean Journal of Environment and Ecology
• /
• v.15 no.3
• /
• pp.230-236
• /
• 2001

The fluxes of wet deposition(WD), throughfall(TF), stemflow(SF) and soil leachates were measured to understand base cation budgets on deciduous ecosystem impacted by acidic deposition in the north-western part of Tomakomai in Hokkaido, Japan. The flux of $H^{+}$ for wet deposition was $0.34kmo1_{c}$ $ha^{2+}$ and the flux of base cation, $K^{+}$ /, Na$^{ + }$, $Ca^{2+}$ and $Mg^{2+}$ far throughfall plus stemflow wart 1.6 kmolc $ha^{-1}$ , 3 times higher level than that for wet deposition. The flux of base cation for canopy leaching(LI) was 0.95 kmolc ha$^{-1}$ , 2.8 times higher level than $H^{+}$ sources in wet deposition. The major mechanism of $^{+}$ consumption closely related to acidic neutralizing capacity of canopy. The ionic flux for soil leachates from Boil reservoir and proton consumption in soil was dependent on soil chemical states and exchangeable Ca in soil had a major factor of H$^{+ }$ consumption. The base cation budgets on deciduous ecosystem showed positive balance fur Na, Ca and Mg, while K was the negative value.

• Ocean and Polar Research
• /
• v.33 no.4
• /
• pp.435-445
• /
• 2011

We investigated geochemical properties, and microbial sulfate- and iron(III) reduction in sediment influenced by the aquaculture of abalone in the Nohwa-do, southern coastal sea in Korea. Concentrations of ammonium, phosphate, and sulfide in the pore-water were higher at farm sites than at control sites. The differences between the 2 types of sites were most apparent in terms of the weights of abalone and the temperature increase during September. Accordingly, the rates of sulfate reduction at the farm sites during September (61 mmol S $m^{-2}d{-1}$) were 3-fold higher than the sulfate reduction during May (20 mmol S $m^{-2}d{-1}$). In contrast, Fe(III) reduction rates were highest at the control sites in May, but its significance was relatively decreased at farm sites during September when sulfate reduction rates were highest. During September, benthic ammonium flux was 3-fold greater at the farm sites (35 mmol N $m^{-2}d{-1}$) than at the control sites (12 mmol N $m^{-2}d{-1}$), and phosphate flux was 8-fold higher at the farm sites (0.018 mmol P $m^{-2}d{-1}$) than at control sites (0.003 mmol P $m^{-2}d{-1}$). Overall results indicated that the inappropriate operation of a large-scale aquaculture farm may result in excess input of biodeposits and high nutrient fluxes from the sediment, thereby decreasing diversity of the benthic ecosystem and deepening eutrophication in coastal waters.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.40 no.3
• /
• pp.167-177
• /
• 2007

We measured the salinity, pH, and concentrations of $^{222}Rn$ and nutrients in groundwater in the southeastern coastal aquifer off Busan from March to September 2005 to evaluate its submarine discharge and geochemical characteristics. Salinity in coastal groundwater increased sharply at 20 m depth and exceeded 25 ppt below 40 m during the study period, indicating that a strong transition zone between fresh groundwater and seawater developed between 20 and 40 m depths. Fresh groundwater in the upper layer of this transition zone was characterized by high pH, $^{222}Rn$, dissolved inorganic nitrogen (DIN), and dissolved inorganic phosphorus (DIP) and low dissolved inorganic silicate (DSi) relative to seawater in the lower layer. In addition, the vertical profiles of the $^{222}Rn$, DIN, and DIP concentrations imply that a strong advective groundwater flow occurs along the interface of fresh groundwater and seawater near 20 m depth. The geochemical constituents in coastal groundwater also showed strong seasonal variation, with the highest concentrations in summer (June 2005) due to the changes of groundwater recharge and sea level. This implies that the input of terrestrial chemical species into the coastal ocean through submarine groundwater discharge (SGD) could change seasonally. To ascertain the seasonal variation of SGD and SGD-driven chemical species fluxes, and associated ecological responses in the coastal ocean, more extensive studies are necessary using various SGD tracers or seepage meters in the future.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2023.05a
• /
• pp.145-145
• /
• 2023

육지의 영양염류는 강수를 통해 강을 따라 바다로 흐른다. 연안 수질과 생태계를 효과적으로 관리하기 위해 수계를 통해 연안으로 배출되는 육상 영양염류 유출의 변화를 연구하는 것이 중요하다. 그러나 제한적인 수량과 수질 동시 관측자료로 인해 한반도 연안으로 유입되는 영양염량 변화에 대한 연구는 매우 제한적이다. 따라서 본 연구에서는 한반도 내륙에서 주변 연안 지역으로 유출되는 영양염류의 유출량과 그 변화량을 추정 및 분석하고 유출량에 대한 수량과 수질 변화의 기여도를 정량적으로 조사하였다. 2012-2021년 봄철(3-5월)에 한반도 연안 주변의 75개의 하천 지점에서 측정된 수질 농도 및 유량 관측 데이터와 한반도 연안 375지점에서 측정된 해양 수질 농도 데이터를 사용하였다. 더불어 22개의 해안지역 하천 유량 관측소와 근접한 수질 관측자료를 통해, 총 질소(TN), 총 인(TP), 클로로필a의 봄철 영양염류 유출 총량을 추정하고 그 변화량을 평가하였다. 그리고 영양염류 유출 변화량을 모델 수식으로 분해하여 유출 총량 변화량에 대한 하천 유량과 영양염류 농도 변화의 상대적인 기여도를 분석하였다. 분석결과는 연간 봄철 TN, TP, 클로로필a 유출 총량의 변화율이 영양염류의 농도 (각각 19%, 48%, 5%)보다는 하천 유량 (각각 84%, 51%, 91%)에서 더 많은 영향을 받았음을 보여주었다. 한반도 연안을 서해, 남해, 동해로 나누어 영양염류 유출 총량을 분석한 결과, 한반도 전체 유출량에서 서해의 가장 큰 기여도를 확인하였다. 또한 한반도 내륙에서 유출된 영양염류 총량이 연안의 수질에 끼치는 영향을 조사하고자 내륙 하천과 연안의 수질 간의 상관관계를 조사하여 간접적으로 파악하였다. 본 연구는 분석적인 접근을 통해 하천과 연안 수질 환경에서 수문학적인 연결고리의 중요성을 강조하고, 영양염류 유출 변화가 해안에 서식하는 생태계와 양식산업 생산성에 미치는 잠재적인 영향을 강조하였다.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.3
• /
• pp.496-510
• /
• 2007

This paper describes the use of a discrete mathematical model to represent the basic mechanisms of regulation of the bacteria E. coli in batch fermentation. The specific phenomena studied were the changes in metabolism and genetic regulation when the bacteria use three different carbon substrates (glucose, glycerol, and acetate). The model correctly predicts the behavior of E. coli vis-a-vis substrate mixtures. In a mixture of glucose, glycerol, and acetate, it prefers glucose, then glycerol, and finally acetate. The model included 67 nodes; 28 were genes, 20 enzymes, and 19 regulators/biochemical compounds. The model represents both the genetic regulation and metabolic networks in an integrated form, which is how they function biologically. This is one of the first attempts to include both of these networks in one model. Previously, discrete mathematical models were used only to describe genetic regulation networks. The study of the network dynamics generated 8 $(2^3)$ fixed points, one for each nutrient configuration (substrate mixture) in the medium. The fixed points of the discrete model reflect the phenotypes described. Gene expression and the patterns of the metabolic fluxes generated are described accurately. The activation of the gene regulation network depends basically on the presence of glucose and glycerol. The model predicts the behavior when mixed carbon sources are utilized as well as when there is no carbon source present. Fictitious jokers (Joker1, Joker2, and Repressor SdhC) had to be created to control 12 genes whose regulation mechanism is unknown, since glycerol and glucose do not act directly on the genes. The approach presented in this paper is particularly useful to investigate potential unknown gene regulation mechanisms; such a novel approach can also be used to describe other gene regulation situations such as the comparison between non-recombinant and recombinant yeast strain, producing recombinant proteins, presently under investigation in our group.