• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.3
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• pp.615-622
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• 2007

In general, Harris detector is commonly used for finding salient points in watermarking systems using feature points. Harris detector is a kind of combined comer and edge detector which is based on neighboring image data distribution, therefore it has some limitation to find accurate salient points after watermark embedding or any kinds of digital attacks. In this paper, we have used cross reference points which use not data distribution but geometrical structure of a normalized image in order to avoid pointing error caused by the distortion of image data. After normalization, we find cross reference points and take inverse normalization of these points. Next, we construct a group of triangles using tessellation with inversely normalized cross reference points. The watermarks are affine transformed and transformed-watermarks are embedded into not normalized image but original one. Only locations of watermarks are determined on the normalized image. Therefore, we can reduce data loss of watermark which is caused by inverse normalization. As a result, we can detect watermarks with high correlation after several digital attacks.

• Journal of the Korean Institute of Oriental Medical Informatics
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• v.15 no.2
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• pp.1-6
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• 2009

The discipline of terminology is based on its own theoretical principles and consists primarily of the following aspects: analysing the concepts and concept structures used in a field or domain of activity, identifying the terms assigned to the concepts, in the case of bilingual or multilingual terminology, establishing correspondences between terms in the various languages, creating new terms, as required. The word properties has syntax, morphology and orthography. The syntax is that how words are put together. The morphology is consist of inflection, derivation, and compounding. The orthography is spelling. Otherwise, the terms of TKM(Traditional Korean Medicine) is two important element of visual character and phonetic notation. A visual character consist of spell, sort words, stop words, etc. For example, that is a case of sort words in which this '다한', '한다', '多汗', '汗多' as same. A phonetic notation consist of palatalization, initial law, etc. For example, that is a case of palatalization in which this '수족랭', '수족냉', '手足冷', '手足冷' as same. Therefore, to enlarged reference terminology is a method by term's normalization. For such a reason, TKM's terms of normalization is necessary.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.12
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• pp.2021-2030
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• 2020

Objective: Quantitative polymerase chain reaction (qPCR) has been extensively used in the field of mesenchymal stem cell (MSC) research to elucidate their characteristics and clinical potential by normalization of target genes against reference genes (RGs), which are believed to be stably expressed irrespective of various experimental conditions. However, the expression of RGs is also variable depending on the experimental conditions, which may lead to false or contradictory conclusions upon normalization. Due to the current lack of information for a clear list of stable RGs in bovine MSCs, we conducted this study to identify suitable RGs in bovine MSCs. Methods: The cycle threshold values of ten traditionally used RGs (18S ribosomal RNA [18S], beta-2-microglobulin [B2M], H2A histone family, member Z [H2A], peptidylprolyl isomerase A [PPIA], ribosomal protein 4 [RPL4], succinate dehydrogenase complex, subunit A [SDHA], beta actin [ACTB], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], TATA box binding protein [TBP], and hypoxanthine phosphoribosyltrasnfrase1 [HPRT1]) in bovine bone marrow-derived MSCs (bBMMSCs) were validated for their stabilities using three types of RG evaluation algorithms (geNorm, Normfinder, and Bestkeeper). The effect of validated RGs was then verified by normalization of lineage-specific genes (fatty acid binding protein 4 [FABP4] and osteonectin [ON]) expressions during differentiations of bBMMSCs or POU class 5 homeobox 1 (OCT4) expression between bBMMSCs and dermal skins. Results: Based on the results obtained for the three most stable RGs from geNorm (TBP, RPL4, and H2A), Normfinder (TBP, RPL4, and SDHA), and Bestkeeper (TBP, RPL4, and SDHA), it was comprehensively determined that TBP and RPL4 were the most stable RGs in bBMMSCs. However, traditional RGs were suggested to be the least stable (18S) or moderately stable (GAPDH and ACTB) in bBMMSCs. Normalization of FABP4 or ON against TBP, RPL4, and 18S presented significant differences during differentiation of bBMMSCs. However, although significantly low expression of OCT4 was detected in dermal skins compared to that in bBMMSCs when TBP and RPL4 were used in normalization, normalization against 18S exhibited no significance. Conclusion: This study proposes that TBP and RPL4 were suitable as stable RGs for qPCR study in bovine MSCs.

• Journal of Internet Computing and Services
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• v.4 no.3
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• pp.9-14
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• 2003

The method of vocal tract normalization is a successful method for improving the accuracy of inter-speaker normalization. In this paper, we present an intra-speaker warping factor estimation based on pitch alteration utterance. The feature space distributions of untransformed speech from the pitch alteration utterance of intra-speaker would vary due to the acoustic differences of speech produced by glottis and vocal tract. The variation of utterance is two types: frequency and amplitude variation. The vocal tract normalization is frequency normalization among inter-speaker normalization methods. Therefore, we have to consider amplitude variation, and it may be possible to determine the amplitude warping factor by calculating the inverse ratio of input to reference pitch. k, the recognition results, the error rate is reduced from 0.4% to 2.3% for digit and word decoding.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2013.05a
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• pp.142-143
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• 2013

Green building certifications and environmental assessments are extensively implemented and studied to decrease the environmental impact during the life cycle of buildings. However, most of them are not appropriate to assess the environmental performance during the construction phase due to the difference of environmental factors. To develop an environmental index of construction projects, normalization should be conducted to compare the relative impact of each factor. As a first step, this study deduced normalization references of 4 environmental factors : noise, waste, greenhouse gas, and dust.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.36 no.6
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• pp.515-523
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• 2018

In order to obtain consistent change detection result for multi-temporal satellite images, preprocessing must be performed. In particular, the preprocessing related to the spectral values can be performed by the radiometric normalization, and relative radiometric normalization is generally utilized. However, most relative radiometric normalization methods assume a linear relationship between the two images, and nonlinear spectral characteristics such as phenological differences are not considered. Therefore, this study proposes a relative radiometric normalization which assumes nonlinear relationships that can perform compositive normalization of radiometric and phenological characteristics. The proposed method selects the subject and reference images, and then extracts the radiometric control set samples through the no-change method. In addition, spectral indexes as well as pixel values are extracted in order to consider sufficient information, and modeling of nonlinear relationships is performed through multilayer perceptron. Finally, the proposed method is compared with the conventional relative radiometric normalization methods, which shows that the proposed method is visually and quantitatively superior.

• The Plant Pathology Journal
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• v.27 no.4
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• pp.301-309
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• 2011

The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination of three statistical algorithms (BestKeeper, geNorm, and NormFinder), the variation in the expression of these genes was assessed under different culture conditions that favored mycelial growth, sexual development, and trichothecene mycotoxin production. When favoring mycelial growth, GzFLO and GzUBH expression were most stable in complete medium. Both EF1A and GzRPS16 expression were relatively stable under all conditions on carrot agar, including mycelial growth and the subsequent perithecial induction stage. These two genes were also most stable during trichothecene production. For the combined data set, GzUBH and EF1A were selected as the most stable. Thus, these genes are suitable reference genes for accurate normalization of qRT-PCR data for gene expression analyses of F. graminearum and other related fungi.

• The Journal of the Acoustical Society of Korea
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• v.3 no.1
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• pp.25-35
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• 1984

This paper analysis the Dynamic Time Warping algorithms for time normalization of speech pattern and discusses the Dynamic Programming algorithm for spoken Korean isolated digits recognition. In the DP matching, feature vectors of the reference and test pattern are consisted of first three formant frequencies extracted by power spectrum density estimation algorithm of the ARMA model. The major differences in the various DTW algorithms include the global path constrains, the local continuity constraints on the path, and the distance weighting/normalization used to give the overall minimum distance. The performance criterias to evaluate these DP algorithms are memory requirement, speed of implementation, and recognition accuracy.

• Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.21.1-21.11
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• 2016

Background: The evaluation of suitable reference genes as normalization controls is a prerequisite requirement for launching quantitative reverse transcription-PCR (RT-qPCR)-based expression study. In order to select the stable reference genes in abalone Haliotis discus hannai tissues (gill and hepatopancreas) under heavy metal exposure conditions (Cu, Zn, and Cd), 12 potential candidate housekeeping genes were subjected to expression stability based on the comprehensive ranking while integrating four different statistical algorithms (geNorm, NormFinder, BestKeeper, and ${\Delta}CT$ method). Results: Expression stability in the gill subset was determined as RPL7 > RPL8 > ACTB > RPL3 > PPIB > RPL7A > EF1A > RPL4 > GAPDH > RPL5 > UBE2 > B-TU. On the other hand, the ranking in the subset for hepatopancreas was RPL7 > RPL3 > RPL8 > ACTB > RPL4 > EF1A > RPL5 > RPL7A > B-TU > UBE2 > PPIB > GAPDH. The pairwise variation assessed by the geNorm program indicates that two reference genes could be sufficient for accurate normalization in both gill and hepatopancreas subsets. Overall, both gill and hepatopancreas subsets recommended ribosomal protein genes (particularly RPL7) as stable references, whereas traditional housekeepers such as ${\beta}-tubulin$ (B-TU) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were ranked as unstable genes. The validation of reference gene selection was confirmed with the quantitative assay of MT transcripts. Conclusions: The present analysis showed the importance of validating reference genes with multiple algorithmic approaches to select genes that are truly stable. Our results indicate that expression stability of a given reference gene could not always have consensus across tissue types. The data from this study could be a good guide for the future design of RT-qPCR studies with respect to metal regulation/detoxification and other related physiologies in this abalone species.

• Journal of Animal Reproduction and Biotechnology
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• v.34 no.4
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• pp.280-291
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• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.