• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1652-1662
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• 2014

The present study investigated the optimum blending condition of protected fat, choline and yeast culture for lowering of rumen temperature. The Box Benken experimental design, a fractional factorial arrangement, and response surface methodology were employed. The optimum blending condition was determined using the rumen simulated in vitro fermentation. An additive formulated on the optimum condition contained 50% of protected fat, 25% of yeast culture, 5% of choline, 7% of organic zinc, 6.5% of cinnamon, and 6.5% of stevioside. The feed additive was supplemented at a rate of 0.1% of diet (orchard grass:concentrate, 3:7) and compared with a control which had no additive. The treatment resulted in lower volatile fatty acid (VFA) concentration and biogas than the control. To investigate the effect of the optimized additive and feed energy levels on rumen and rectal temperatures, four rumen cannulated Hanwoo (Korean native beef breed) steers were in a $4{\times}4$ Latin square design. Energy levels were varied to low and high by altering the ratio of forage to concentrate in diet: low energy (6:4) and high energy (4:6). The additive was added at a rate of 0.1% of the diet. The following parameters were measured; feed intake, rumen and rectal temperatures, ruminal pH and VFA concentration. This study was conducted in an environmentally controlled house with temperature set at $30^{\circ}C$ and relative humidity levels of 70%. Steers were housed individually in raised crates to facilitate collection of urine and feces. The adaptation period was for 14 days, 2 days for sampling and 7 days for resting the animals. The additive significantly reduced both rumen (p<0.01) and rectal temperatures (p<0.001) without depressed feed intake. There were interactions (p<0.01) between energy level and additive on ruminal temperature. Neither additive nor energy level had an effect on total VFA concentration. The additive however, significantly increased (p<0.01) propionate and subsequently had lower acetate:propionate (A/P) ratios than non-additive supplementation. High concentrate diets had significantly lower pH. Interactions between energy and additive were observed (p<0.01) in ammonia nitrogen production. Supplementation of diets with the additive resulted in lower rumen and rectal temperatures, hence the additive showed promise in alleviating undesirable effects of heat stress in cattle.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.39 no.4
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• pp.106-116
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• 2016

MTO (Make to Order) is a manufacturing process in which manufacturing starts only after a customer's order is received. Manufacturing after receiving customer's orders means to start a pull-type supply chain operation because manufacturing is performed when demand is confirmed, i.e. being pulled by demand (The opposite business model is to manufacture products for stock MTS (Make to Stock), which is push-type production). There are also BTO (Build to Order) and ATO (Assemble To Order) in which assembly starts according to demand. Lean manufacturing by MTO is very efficient system. Nevertheless, the process industry, generally, which has a high fixed cost burden due to large-scale investment is suitable for mass production of small pieces or 'mass customization' defined recently. The process industry produces large quantities at one time because of the lack of manufacturing flexibility due to long time for model change or job change, and high loss during line-down (shutdown). As a result, it has a lot of inventory and costs are increased. In order to reduce the cost due to the characteristics of the process industry, which has a high fixed cost per hour, it operates a stock production system in which it is made and sold regardless of the order of the customer. Therefore, in a business environment where the external environment changes greatly, the inventory is not sold and it becomes obsolete. As a result, the company's costs increase, profits fall, and it make more difficult to survive in the competition. Based on the customer's order, we have built a new method for order system to meet the characteristics of the process industry by producing it as a high-profitable model. The design elements are designed by deriving the functions to satisfy the Y by collecting the internal and external VOC (voice of customer), and the design elements are verified through the conversion function. And the Y is satisfied through the pilot test verified and supplemented. By operating this make to order system, we have reduced bad inventories, lowered costs, and improved lead time in terms of delivery competitiveness. Make to order system in the process industry is effective for the display glass industry, for example, B and C groups which are non-flagship models, have confirmed that the line is down when there is no order, and A group which is flagship model, have confirmed stock production when there is no order.

• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.285-290
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• 2007

This study was conducted to introduce phosphinothricin acetyl transferase (PAT) gene, resistant to basta which was non-selective herbicide, into balloon flower (Platycodon grandiflorum A. De. candolle). Seeds were germinated on MS medium, and 10-day-old immature cotyledon explants and 30-day-old leaf explants were cocultured with Agrobacterium tumefaciens strain MP 90 (pBinSyn) on 1/10 MS medium for 48 hours in the dark at $25^{\circ}C$. The cultures were transferred for selection of kanamycin-resistant shoots to the MS medium supplemented with 0.2 $mg/{\ell}$ NAA, 1.0 $mg/{\ell}$ BA, 3% sucrose, 100 $mg/{\ell}$ kanamycin, 500 $mg/{\ell}$ carbenicillin. Shoots were obtained from 10-day-old immature cotyledon explants after 4 weeks of culture. The shoots were subcultured twice every 4 weeks on the same medium for growth of transgenic shoots. Successful transformation was confirmed by histochemical GUS assay, PCR analysis, RT-PCR analysis, 10 $mg/{\ell}$ phosphinothricin treatment and 0.3% basta spray. The basta-resistant transgenic plants flowered normally.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.8
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• pp.1145-1151
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• 2016

This study was conducted to evaluate the relative bioavailability (RBV) of 25-hydroxycholecalciferol (25-OH-$D_3$) to cholecalciferol (vitamin $D_3$) in 1- to 21-d-old broiler chickens fed with calcium (Ca)- and phosphorus (P)-deficient diets. On the day of hatch, 450 female Ross 308 broiler chickens were assigned to nine treatments, with five replicates of ten birds each. The basal diet contained 0.50% Ca and 0.25% non-phytate phosphorus (NPP) and was not supplemented with vitamin D. Vitamin $D_3$ was fed at 0, 2.5, 5.0, 10.0, and $20.0{\mu}g/kg$, and 25-OH-$D_3$ was fed at 1.25, 2.5, 5.0, and $10.0{\mu}g/kg$. The RBV of 25-OH-$D_3$ was determined using vitamin $D_3$ as the standard source by the slope ratio method. Vitamin $D_3$ and 25-OH-$D_3$ intake was used as the independent variable for regression analysis. The linear relationships between the level of vitamin $D_3$ or 25-OH-$D_3$ and body weight gain (BWG) and the weight, length, ash weight, and the percentage of ash, Ca, and P in femur, tibia, and metatarsus of broiler chickens were observed. Using BWG as the criterion, the RBV value of 25-OH-$D_3$ to vitamin $D_3$ was 1.85. Using the mineralization of the femur, tibia, and metatarsus as criteria, the RBV of 25-OH-$D_3$ to vitamin $D_3$ ranged from 1.82 to 2.45, 1.86 to 2.52, and 1.65 to 2.05, respectively. These data indicate that 25-OH-$D_3$ is approximately 2.03 times as active as vitamin $D_3$ in promoting growth performance and bone mineralization in broiler chicken diets.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.857-864
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• 2009

This experiment was conducted to investigate the effects of interaction between $1{\alpha}$-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) and phytase on growth performance, parameters of tibia and plasma, and meat quality of 1- to 21-d-old broilers. Two hundred and forty male, 1-d-old Arbor Acres broilers were randomly assigned to 20 cages, with 12 chicks per cage. Five treatments were designed, with four cages each. A 2${\times}$2 factorial experiment was designed to test 0 and 5 ${\mu}g/kg$ of $1{\alpha}$-OH $D_3$ in combination with 0 and 500 U/kg of phytase. A basal diet was formulated to contain 2.9 g/kg of non-phytate phosphorus (NPP), and the control diet was formulated to contain a normal level of NPP (4.5 g/kg). Results showed that $1{\alpha}$-OH $D_3$ alone increased tibia ash, contents of calcium and phosphate, breaking strength, concentrations of plasma calcium and phosphate, and water-holding capacity of breast and thigh meat, while it decreased growth of broilers. Phytase alone improved performance and tibia quality. Although growth of broilers was lower than that of the positive control when the diet was supplemented with $1{\alpha}$-OH $D_3$ and phytase, tibia quality was significantly improved by the addition of $1{\alpha}$-OH $D_3$ and phytase. These data suggest that interaction between $1{\alpha}$-OH $D_3$ and phytase at 2.9 g/kg of dietary NPP could significantly increase bone quality of 1- to 21-d-old broilers, while not improving growth performance.

• Journal of Embryo Transfer
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• v.23 no.4
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• pp.269-274
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• 2008

The purpose of this study was to determine the effects of Taxol pre-treatment to in vitro matured bovine oocytes, and sucrose and trehalose added to vitrification solution on spindle morphology and embryonic development following cryopreservation. Bovine oocytes were collected from ovaries and matured in tissue culture medium 199 (TCM 199) supplemented with 10% Fetal Bovine Serum (FBS), 0.05ng/ml epidermal growth factor, 0.01 IU/ml luteinizing hormone and $1{\mu}g/ml$ estradiol for 22h in $39^{\circ}C$, 5% $CO_2$, TCM 199-HEPES containing 20% FBS was used as basic medium (BM) to prepare vitrification solution. Oocytes were pre-treated with $1\;{\mu}M$ Taxol in maturation medium for 15 min prior to vitrification. Oocytes were exposed to 1.6 M ethylene glycol (EG) and 1.3M dimethyl sulfoxide (DMSO) in BM and then were exposed to 3.2 M EG, 2.6 M DMSO and 0.5 M sucrose in BM or 3.2 M EG, 2.6 M DMSO and 0.5 M trehalose in BM. Oocytes with cumulus cells and oocytes without cumulus cells were considered as control 1 and control 2, respectively and held in TCM 199-HEPES at $39^{\circ}C$. Oocytes were frozen using modified solid surface vitrification and were stored in cryotubes in liquid nitrogen for more than 1 week. Frozen oocytes were thawed in TCM 199-HEPES containing 0.5 M, 0.25 M and 0.1 M sucrose in BM for 2 min, respectively or 0.5 M, 0.25 M and 0.1 M trehalose in BM for 2 min, respectively. Immunoflurorescence staining of oocytes was performed to assess spindle morphology and chromosome configuration of oocytes. The rates of cleavage and blastocyst were examined following in vitro fertilization. Normal spindle morphology rate of oocytes pre-treated with Taxol prior to vitrification was not higher than that of other vitrified groups. Taxol pre-treatment did not increase cleavage and blastocyst formation rates, although control groups showed significantly higher rates (p<0.05). Percentages of normal spindle and embryonic development were not significantly different among vitrified groups regardless of type of sugar. In conclusion, Taxol pre-treatment of oocytes before cryopreservation did not reduce the damage induced by vitrification and subsequently did not improve embryonic development following vitrification. Trehalose may be used as an alternative non-permeating cryoprotectant in vitrification solution.

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.48-53
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• 2000

Lacquer tree can be proliferated by root or stem cutting, and seed. In case of proliferation by seed, however, the germination rate is very low. Thus, the present study was carried out to obtain aspceptic lacquer plant in vitro from seed because natural tissue culture was highly defiled by unknown fungi and bacteria. First seed grading on distilled water was 50.7% and second seed grading was 20.8% after 98% sulfuric acid treatment for 2 hours. Removal of inner seed coat was higher with 32.4% than non-removal of outer seed coat and removal outer seed coat in rooting rate. In germination rate according to pre-treatment, growth regulators were not effective at all, but sulfuric acid was effective a little with 3%. Removal outer seed coat was increased about 4%, that germinated about 10% in MS medium supplemented with 1.0mg/L BA and 0.05mg/L NAA, 1.0mg/L BA. Lacquer tree seeds germinated after 10 days in MS medium, and aspceptic seedling of lacquer tree were obtained after 3 weeks in vitro. Germination rate, however, was lower about 10%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.2
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• pp.211-216
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• 2009

This study was conducted to determine whether Pleurotus ostreatus (oyster mushroom), cultivated in various ratios with herbal extract remnants instead of cotton supplemented with nutrients (the control), improved mycelial growth, mushroom yields and longevity during storage. In addition, we investigated the transfer of medicinal herb components into the mushrooms since they contained non-specific medicinal herbs and their composition could not be controlled. Mushrooms cultivated with 70% and 100% medicinal herb remnants had faster growth rates, higher yields and less failure in the development of the fruit body than the control group. There were no differences in HPLC chromatogram among the methanol extracts of Pleurotus ostreatus in all groups. In addition, glycyrrhizin, an indicative compound of licorice which was a major herb among the herbal remnants, was not detected in any of the extracts. Pleurotus ostreatus that was cultivated with 70% and 100% herbal extract remnants had improved storage longevity in comparison with the control. They exhibited the least weight loss during storage among the groups and they maintained firmness in the stipe and pileus. However, the sources of media did not alter the color difference of the stipe and pileus or the quality index of the outward appearance during storage. In conclusion, cultivating media that contained over 70% of medicinal herb extract remnants increased the growth rates and yields of Pleurotus ostreatus. In addition, these mushrooms had enhanced storage longevity due to their firmness. Therefore, medicinal herb extract remnants should be utilized in the cultivating media of various mushrooms.

• Korean Institute of Interior Design Journal
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• v.26 no.4
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• pp.42-56
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• 2017

In Korea, which has three sides of the sea, various waterfront spaces are being developed and developed along with various coastal landscapes. However, there are a lot of cases that do not consider the regional characteristic of the coast or the characteristic of the coastal landscape. In many cases, the street facilities cause the landscape degradation and the use inconvenience because of incongruity with the scenery, aging and lax management. Therefore, it is necessary to reflect the specificity of the coastal landscape in planning and directing the street facilities, harmonizing with the surrounding natural environment, and consideration of sustainability. However, there is no research or planning index for sustainable street facilities considering the coastal characteristics. Therefore, this study suggests plan elements to improve the sustainability of the street facilities on the shore and proposes them as basic plan elements, and suggests what kind of improvements should be made to improve the sustainability of coastal facilities in Korea. Based on the plan elements derived from the plan, we checked the problems and complementary points of coastal street facilities in Korea by analyzing the current status of the street facilities around the Jeju Island beach from a sustainable point of view, and suggested plan indicators that should be supplemented to improve sustainability. We surveyed the street facilities such as walkway blocks, benches, pagoda, drinking fountain, trash cans, bicycle shelters, fences, guide maps, streetlights, and shower rooms in 10 beaches in Jeju Island. This study analyzed the sustainability characteristics of the each factor of these facilities by surveying the reflectivity of the 23 plan indicators from the six aspects derived from the sustainability planning factor as conformity, partial reflection, non - reflection, and nonconformity. The results of the analysis showed that the functional aspect was the highest with 68.3%, the material aspect (53%), the landscape aspect (22.3%), the productive aspect (14.7%) and the morphological aspect (12.2%), and maintenance / management (10.6%). In addition, the facilities with the lowest fitness in each aspect are bicycle shelter in landscape aspect, bicycle storage box and trash can in form aspect, shower room in material aspect, trash can in functional aspect, showroom, guide maps and drinking fountain in product aspect, showroom In maintenance aspect. In order to improve the sustainability of coastal facilities in the future, it is necessary to complement the administrative and landscape aspects. In coastal facilities, it is necessary to consider the sustainability planning index, especially for showers, taking into account the specificity of beaches. Consideration should be given to various aspects of sustainability for bike shelters and trash facilities. Through this study, we have proposed the plan elements to systematize, supplement, and improve the planning index for sustainability improvement in coastal facilities planning.

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.15-19
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• 2012

A number of researcher have studied biomaterials for cartilage regeneration and are now proceeding. Silk protein was attempted for use as biomedical materials by many researchers because it is natural polymer with biocompatibility and excellent mechanical strength. In this study, we want to know a possibility of silk protein on the cartilage regeneration. We isolated chondrocytes from nasal cartilage and confirmed optimal culture condition of the cells. To observe the effects of silk fibroin on chondrogenesis, we added silk fibroin solutions to the culture medium of chondrocyte and detected gene expression levels related chondrogenesis such as col2, col10. The chondrocytes showed optimal growth when they were cultured in DMEM medium supplemented with 10% FBS 100 ${\cdot}{\ddot{I}}$M ascorbic acid. The levels of col2 gene expression were increased in non-autoclaved silk fibroin, but decreased in autoclaved one. Also the gene expression levels of col10 were increased in silk fibroin, particulary at 3D culture. Based on the results of this study, we had seen the possibility of silk fibroin for cartilage regeneration. In future studies, we should know more clearly the relationship between cartilage regeneration and the silk protein.