• BMB Reports
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• v.37 no.3
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• pp.339-342
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• 2004

Germination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively abeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.

• Restorative Dentistry and Endodontics
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• v.16 no.2
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• pp.182-188
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• 1991

The purpose of this study was to clarify the effect of intracana1 medication on the clinical symptoms and the number of visits before canal filling in endodontic treatment The experimental teeth was divided into three groups. The teeth of group 1 was irrigated with saline solution and the teeth of group 2 was irrigated with sodium hypochlorite during biomechanical preparation. The intracanal medicament was not applied in the canals of group 1 and group 2. The teeth of group 3 was irrigated with sodium hypochlorite and hydrogen peroxide during canal enlargement and applied formocresol as intracanal medicament after drying the root canals with paper points. The incidence of interappintment pain and percussion pain after 1 st visit and the number of appointments before canal filling was examined, and the results was statistically compared. The following results were obtained. 1. The incidence of interappointment pain and percussion pain and the number of visits before canal filling was not effected by the types of irrigant and the use of the intracanal medicament 2. There was no significant difference in the incidence of interappintment pain arid percussion pain and the number of appointement before canal filling between vital teeth and non vital teeth, and also between single rooted teeth and multirooted teeth. 3. The use of formocresol as intracanal medicament was not able to aid the elimination of clinical symptoms and to decrease the number of appointment before canal filling.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.29 no.2
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• pp.36-45
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• 1997

Newly bleaching sequence using oxygen, ozone and hydrogen peroxide has introduced to avoid pollution hazards from chlorinated organic compounds, because chlorine dioxide substitution bleaching was produced a little chlorinated organic substance. Oxygen-type chemicals replaced for chlorine has attracted much research attention. Bleachability of ozone was improved at low temperature and high pulp consistency. In third bleaching followed OZ bleaching, addition of O2 and NaBH4 in alkali extraction was effective than only alkali extraction. Bleachability of pulps in ozone bleaching(Z) was improved at higher consistency and lower temperature The addition O2 and NaBH4 in alkali extraction after OZ bleaching sequence improved brightness, when compared to those obtained by only alkaline extraction. Pulps bleached by ECF bleaching sequences such as OZEoD and OZEopD was obtained by 90% ISO brightness. The brightness of pulp bleached by TCF sequences with the ozone dosage of 1.6% was approached to target brightness (88~90%ISO). Pulps bleached Z stage combined bleaching sequence was reduced the viscosity to a little greater extent. However, physical properties of pulps was not great different compared to those bleached by conventional bleaching sequences. A pollution index of bleaching effluente by ozone combined bleaching sequences was lower than by conventional bleaching sequence, but somewhat higher than multistage bleaching sequences combined C/D stage.

• Journal of Nutrition and Health
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• v.32 no.7
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• pp.775-780
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• 1999

We investigated the effects of antioxidant vitamins supplementation on antioxidative status and plasma lipid profile in female Korean non-insulin diabetes mellitus(NIDDM) patients. Forty-five patients were groups by types of vitamin to take into three groups-Vitam in E group(400IU/day, n=15), Vitamin C group (1,000mg/day, n=15) and Vitamin E plus C group (400 IU plus 1,000mg/day). Supplementation period was 4 weeks. After vitamins supplementation, plasma vitamin E concentration significantly increased in vitamin E and vitamin E + C group, but plasma retinol concentration were not affected by vitamin E or vitamin C supplementation. And plasma levels of lipid peroxide measured as thiobarbituric acid reactive substances(TBARS), indicator of lipid peroxidation and increased susceptibility of LDL towards lipid peroxidation, were significantly decreased in all three groups after vitamins supplementation. Also catalase activities in erythrocytes were significantly decreased after antioxidant vitamin supplementation in all subjects. And after vitamins supplementation, post prandial 2 hour glucose and total cholesterol levels was decreased in all subjects. And after vitamins supplementation, post prandial 2 hour glucose and total cholesterol levels was decreased in all patients, especially there was a significant difference in vitamin C, Vitamin E + C group. In this study, antioxidant vitamins supplementation might have a protective function against the free radical-mediated lipid peroxidation and decrease the plasma total cholesterol levels in Korean female NIDDM patients.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.1
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• pp.27-28
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• 1973

The activities of peroxidase and polyphenoloxidase were investigated in the rice leaves(the upper halves) diseased with Akagare or Helminthosporium oryzae. The activity of polyphenoloxidase was slightly lower than that of peroxidase in the healthy leaves but it increased 56% in the diseased leaves while peroxidase decreased 35%. It was expected that polyphenoloxidase is dominant in the oxidation of polyphenols, and hydrogen peroxide may accumulate to harmful level due to the decrease of peroxidase activity resulting in non-enzymatic oxidation of polyphenols in the diseased leaves.

• Korean Journal of Oriental Medicine
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• v.3 no.1
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• pp.199-213
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• 1997

In convulsion state by PTZ in rat, anticonvulsive effect and some of ${\gamma}-aminobutyric$ acid(GABA)-related mechanisms of Buthus extract in brain was experimented. It was inhibited GABA-T activity, lipid peroxide generation and xanthine oxidase activity as scheduled administration in vitro and vivo. And the content of brain glutathione was increased as scheduled administration in rat. In convulsion state by PTZ of previously managed rat by Buthus extract, onset time and duration were non-specific changes but recovery time and severity was remarkably reduced. In conclusion speculated that Buthus extract inhibits convulsion by control of GABA content In brain.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.2
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• pp.124-130
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• 2016

To effectively remove salts from Suaeda japonica, extracts, an electrodialysis system was developed. The biological activities of non-desalted (NDS) and desalted S. japonica (DS) extracts were compared. The DS extract exhibited superior polyphenolic (6.26%) and carbohydrate (28.56%) contents. The IC₅₀ values of the DS extract against DPPH radicals and hydrogen peroxide were 0.22 and 0.68 mg/mL, respectively, which was higher than that of the NDS extract. Neither the DS nor the NDS extract was cytotoxic in RAW 264.7 macrophages. Additionally, the DS extract had a higher NO inhibitory effect compared to the NDS extract in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. These data indicate that DS extracts have greater biological activity than do ND extracts, and application of the electrodialysis process may be useful in marine bioresource applications.

• Journal of Korean Society on Water Environment
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• v.30 no.6
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• pp.653-657
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• 2014

Effects of $H_2O_2$ addition for fenton oxidation on iron coagulation for treatment of phosphorus species, such as orthophosphate, metaphosphate, pyrophosphate, organic phosphate, were investigated. The effects of coagulant dosage, hydrogen peroxide dosage and the combined sequence ferric coagulation and $H_2O_2$ addition for fenton oxidation and coagulation were studied. The characteristics of floc growth rate were monitored using the PDA. The removal efficiencies of phosphorus species by iron coagulation were increased as Fe/P molar ratio increased. However, the removal efficiencies of metaphosphate, pyrophosphate, organic phosphate by a ferric coagulation were not increased as Fe/P molar ratio increased. The removal efficiency of metaphosphate, pyrophosphate, organic phosphate was increased by using iron coagulation and $H_2O_2$ addition for fenton oxidation. The result indicated that non-reactive phosphorus after iron coagulation was changed to reactive phosphorus by $H_2O_2$ addition for fenton oxidation and the oxidized iron enhanced the coagulation efficiencies.

• Journal of the Korean Ceramic Society
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• v.46 no.6
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• pp.561-567
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• 2009

The Fe-ACF/$TiO_2$ composites were prepared by a sol-gel method and were characterized by nitrogen adsorption, Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), and Energy Dispersive X-ray (EDX). The Fe-ACF/$TiO_2$ composites were developed for the decomposition of organic dyes by using a UV lamp. The decomposition effect was investigated under various conditions, such as three selected non-biodegradable organic dyes like Methylene Blue (MB), Methyl Orange (MO), Rhodamine B (Rh.B), and in the presence of Fe and hydrogen peroxide ($H_2O_2$). The photocatalytic activity was derived from possible combination effects, such as (1) adsorption of ACF, (2) generation of electron/hole by $TiO_2$, (3) photo-Fenton reaction of Fe, and (4) oxidation of $Fe^{2+}\;to\;Fe^{3+}\;by\;H_2O_2$.

• Journal of Applied Biological Chemistry
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• v.50 no.4
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• pp.227-233
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• 2007

We characterized a full-length cDNA of CaCOP1 from pepper. Phylogenetic analysis based on the deduced amino acid sequence of CaCOP1 cDNA revealed high sequence similarity to the COP1 gene in Oryza sativa (84% identity). CaCOP1 shares high sequence identity with regulatory protein in Arabidopsis (84%), constitutively photomorphogenic 1 protein in Pisum sativum (81%) and COP1 homolog in Lycopersicon esculentum (79%). CaCOP1 gene exists single copy in the chili pepper genome. Expression of CaCOP1 was reduced in response to inoculation of non-host pathogens. The expression of this gene under abiotic and oxidative stresses was investigated, including 200 mM NaCl, 200 mM mannitol, cold ($4^{\circ}C$), 100 ${\mu}M$ abscisic acid (ABA), and 10 mM hydrogen peroxide ($H_2O_2$). CaCOP1 was induced significantly 3 h after low temperature treatment but not by dehydration or high salinity. Moreover, CaCOP1 was not induced by plant hormone ABA. These observations suggest that CaCOP1 gene plays a role in abiotic stress and may be belong to ABA-independent regulation system.