• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.674-680
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• 2013

Yeast Dekkera/Brettanomyces bruxellensis is probably the most common contaminant in wineries and ethanol production processes. The considerable economic losses caused by this yeast, but also its ability to produce and tolerate high ethanol concentrations, make it an attractive subject for research with potential for industrial applications. Unfortunately, efforts to understand the biology of D. bruxellensis and facilitate its broader use in industry are hampered by the lack of adequate procedures for delivery of exogenous DNA into this organism. Here we describe the development of transformation protocols (spheroplast transformation, LiAc/PEG method, and electroporation) and report the first genetic transformation of yeast D. bruxellensis. A linear heterologous DNA fragment carrying the kanMX4 sequence was used for transformation, which allowed transformants to be selected on plates containing geneticin. We found the spheroplast transformation method using 1M sorbitol as osmotic stabilizer to be inappropriate because sorbitol strikingly decreases the plating efficiency of both D. bruxellensis spheroplast and intact cells. However, we managed to modify the LiAc/PEG transformation method and electroporation to accommodate D. bruxellensis transformation, achieving efficiencies of 0.6-16 and 10-20 transformants/${\mu}g$ DNA, respectively. The stability of the transformants ranged from 93.6% to 100%. All putative transformants were analyzed by Southern blot using the kanMX4 sequence as a hybridization probe, which confirmed that the transforming DNA fragment had integrated into the genome. The results of the molecular analysis were consistent with the expected illegitimate integration of a heterologous transforming fragment.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.05a
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• pp.437-440
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• 2009

We propose a fast stream cipher ASC16 for software implementation. ASC16 has a very simple structure with ASR(Arithmetic Shift Register), NLF(Non-Linear Filter), and NLB(Non-Linear Block), and is executed by a word. It is a stream cipher for wireless communication, which makes 32bit key streams using s-box with non-linear transformation. The processed result is almost same as SSC2, 32bit output stream cipher, developed by Zhang, Carroll, and Chan. The period is longer than SSC2, and it causes the difficulty of Correlation attack and raises security very much. The proposed ASC16 is efficiently used in the process of a fast cipher in the limited environment such as wireless communication.

• Proceedings of the Korean Society of Laser Processing Conference
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• 2005.06a
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• pp.3-8
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• 2005

The robot systems are widely used in the welding manufacturing. The essential tasks to operate the welding robot are the acquisition of the position and/or shape of the parent metal. For the seam tracking or the robot automation, many kinds of contact and non-contact of the system which monitors the shape of the welding part is described. This system uses the line-type structured laser diode and the visual sensor. It includes the correction of radial distortion which is often found in the image from the camera with short focal length. Direct Linear Transformation (DLT) is used for the camera calibration. The three dimensional shape of the parent metal is obtained after simple linear transformation. Therefore, the system operates in real time. Some experiments are carried out to evaluate the performance of the developed system.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.5 no.2
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• pp.47-55
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• 1987

In this paper, the direct linear transformation is described in which a inner and exterior orientation parameters are treated as unknown for non-iterative direct space resection, and the computer program was developed to obtain object space coordinates. Image coordinates measurements are conducted with analogue stereo-plotter and digitizer. To prove the appropriateness of the two image coordinate measurement devices and the DLT method, the standard errors of object space coordinates are compared with semi-analytical method.

• Journal of Korean Port Research
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• v.12 no.1
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• pp.75-86
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• 1998

To design a coastal structure in the nearshore region, engineers must have means to estimate wave climate. Waves, approaching the surf zone from offshore, experience changes caused by combined effects of bathymetric variations, interference of man-made structure, and nonlinear interactions among wave trains. This paper has attempted to find out the effects of two of the more subtle phenomena involving nonlinear shallow water waves, amplitude dispersion and secondary wave generation. Boussinesq-type equations can be used to model the nonlinear transformation of surface waves in shallow water due to effect of shoaling, refraction, diffraction, and reflection. In this paper, generalized Boussinesq equations under the complex bottom condition is derived using the depth averaged velocity with the series expansion of the velocity potential as a product of powers of the depth of flow. A time stepping finite difference method is used to solve the derived equation. Numerical results are compared to hydraulic model results. The result with the non-linear dispersive wave equation can describe an interesting transformation a sinusoidal wave to one with a cnoidal aspect of a rapid degradation into modulated high frequency waves and transient secondary waves in an intermediate region. The amplitude dispersion of the primary wave crest results in a convex wave front after passing through the shoal and the secondary waves generated by the shoal diffracted in a radial manner into surrounding waters.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1994.04a
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• pp.57-64
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• 1994

The purpose of this paper is to find minimum surface shape of pneumatic structure using the finite element method. The pneumatic membrane structure is a kind of large deformation problem and very flexible composite material, which mean geomatric nonlinearity. It is not to resist for compression and resultant moment. As the displacement due to internal pressure is getting bigger, it should be considered the direction of forces. It becomes non-linear problem with the non-conservative force. The follower-force depends on the deformation and the direction of force is normal to each element. The solution process is obtained the new stiffness matrix (load correction matrix) depending on deformation through each iterated step. However, the stiffness matrix have not the symmetry and influence on the time of covergence. So in this paper Newton-Rhapson method for solving non-linear problem and for using symmetic matrix, the load direction is changed in each iterated step using the transformation matrix.

• Proceedings of the KSRS Conference
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• 2004.10a
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• pp.233-236
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• 2004

In this study, we analyzed the accuracy of the conversion from DLT parameters to physical camera parameters and optimized the use of DLT model for non-metric cameras in photogrammetric tasks. Using the simulated data, we computed two sets of physical camera parameters from DLT parameters and Bundle adjustment for various cases. Comparing two results based on the RMSE values of check points, we optimized the arrangement of GCPs for DLT.

• Proceedings of the KIEE Conference
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• 2002.06a
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• pp.161-165
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• 2002

The paper presents a diagnostic tool for analyzing the bit intermodulation in D/A converter. Bit intermodulation cause linearity errors which degrade the performance of the converter. A linear transformation of the Walsh transform of the integrated non-linearity diagram is shown to be sufficient to extract the bit intermodulation terms and their noise sensitivity. Practical applicability of the proposed method is shown by measurement.

• Journal of the Korean Institute of Intelligent Systems
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• v.11 no.7
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• pp.628-632
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• 2001

We propose time-frequency (TF) tools for analyzing linear time-varying (LTV) systems and nonstationary random processes. Obtained warping the narrowband Weyl symbol (WS) and spreading function (SF), the new TF tools are useful for analyzing LTV systems and random processes characterized by generalized frequency shifts, This new Weyl symbol (WS) is useful in wideband signal analysis. We also propose WS an tools for analyzing systems which produce dispersive frequency shifts on the signal. We obtain these generalized, frequency-shift covariant WS by warping conventional, narrowband WS. Using the new, generalized WS, we provide a formulation for the Weyl correspondence for linear systems with instantaneous of linear signal transformation as weighted superpositions of non-linear frequency shifts on the signal. Application examples in signal and detection demonstrate the advantages of our new results.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.10
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• pp.2207-2216
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• 2012

Recently, wireless Internet environment with mobile phones and wireless sensor networks with severe resource restrictions have been actively studied. Moreover, an overall security issues are essential to build a reliable and secure sensor network. One of secure solution is to develop a fast cryptographic algorithm for data encryption. Therefore, we propose a 128-bit stream cipher, AA128 which has efficient implementation of software and hardware and is suitable for real-time applications such as wireless Internet environment with mobile phones, wireless sensor networks and Digital Right Management (DRM). AA128 is stream cipher which consists of 278-bit ASR and non-linear transformation. Non-linear transformation consists of Confusion Function, Nonlinear transformation(SF0 ~ SF3) and Whitening. We show that the proposed stream cipher AA128 is faster than AES and Salsa20, and it satisfies the appropriate security requirements. Our hardware simulation result indicates that the proposed cipher algorithm can satisfy the speed requirements of real-time processing applications.