• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.125-130
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• 2006

In order to utilize non-commercial melon as alcoholic beverage material, in this study the optimum fermentation method and manufacture conditions were investigated and thereafter the properties of final product were determined. As for melon wine made with only melon juice, sensorial properties, particularly flavor, were not acceptable as alcoholic beverage. To improve the lack of melon wine, we made mash with cooked rice and Nuruk, and then added melon juice during fermentation. Acceptable sensorial properties were obtained in melon wine when 30% extract juice of oriental melon was added after 4 day of fermentation. The alcoholic beverage added melon juice showed much higher yellowness value than that without melon juice indicating that a clear yellow color similar to melon was actually observed in final product. Adding melon juice led little changes in amount and composition of free sugars, organic acids and amino acids and could improve sensorial properties.

• 한국식품과학회지
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• 제53권5호
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• pp.593-600
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• 2021

본 연구에서는 진공 증류 공정으로 무알코올 와인을 제조하는 과정에서 수율은 최대화하고 알코올만 선별적으로 제거하기 위해 반응표면분석법으로 공정을 최적화하였다. 이를 위해 증류 시간, 끓는점, 온도차를 독립변수로 설정하고 알코올 농도, 수율을 반응값으로 설정하였다. 그 결과, 증류 시간은 24.5분, 끓는점은 65℃, 온도차는 8℃ 일 때 알코올 농도를 1%까지 낮추면서 수율을 81.15%로 유지할 수 있었다. 끓는점에 따른 와인의 이화학적 및 관능적 특성 변화를 확인하기 위해 회귀분석 결과를 통해 도출된 반응표면모델을 활용하여 증류 시간 30분, 온도차 12±5℃, 끓는점 25-65℃의 조건에서 세 종류의 무알코올 와인을 제조하였다(끓는점 25℃, 끓는점 45℃, 끓는점 65℃). 또한, 기호도 향상을 위해 끓는점 25℃ 복원액에 원액 와인을 4.2% 첨가한 배합 와인을 제조하였다. 끓는점이 증가할수록 알코올 농도가 감소하고 CI와 Hue가 증가했지만, pH와 총산도는 영향을 받지 않았다. 관능검사에서는 색상, 향, 전체적인 기호도에서 배합 와인이 가장 높은 값을 나타냈고, 끓는점 65℃ 복원액이 가장 낮은 값을 나타냈다. 결과적으로 낮은 온도 및 끓는점에서 증류함으로써 관능 특성이 우수한 무알코올 와인을 제조할 수 있으며, 원액 와인의 배합으로 기호도를 더욱 향상시킬 수 있을 것으로 판단된다.

• 한국미생물·생명공학회지
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• 제43권3호
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• pp.227-235
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• 2015

발효식품에서 분리한 야생 효모 12 균주의 주류 제조 가능성을 조사하기 위해, 알코올 발효에 관여하는 특성을 분석하였다. 알코올 및 당 내성을 조사한 후, 당화액을 제조하여 pH, 고형분 함량, 적정산도, 아미노산도, 알코올, 유기산 및 향기성분 등을 분석하였다. S. cerevisiae 효모의 알코올 내성은 10%에서 보였으나 non-Saccharomyces 효모는 P. kudriavzevii N77-4를 제외하고 내성이 낮았다. 당 내성은 H. opuntiae HP1-2, C. tropicalis Y447을 제외한 효모에서 모두 우수하였다. 야생 효모를 이용한 알코올 발효액의 pH는 3.01−3.61, 고형분 함량은 8.73−13.10°Brix로 S. cerevisiae보다 non-Saccharomyces가 높았다. 적정산도는 0.25−0.33%로, 아미노산도는 1.61−2.61 g/100 ml으로 non-Saccharomyces 효모가 높은 값을 나타내었다. 알코올 함량은 2.25−8.5%로 S. cerevisiae에 비해 non-Saccharomyces 효모가 매우 낮았다. 야생 효모로 알코올 발효시 유기산은 사과산, 초산, 호박산 등이 증가하였고, 그 중 P. kudriavzevii N77-4는 낮은 초산 함량과 높은 사과산 및 호박산 함량을 나타내었다. 당화액을 전자코로 분석한 결과, DF1의 오른쪽 범주에 속했으며, 효모 발효액은 DF1의 왼쪽 범주에 속하여 발효에 따른 뚜렷한 차이를 보였다. 휘발성 향기성분은 non-Saccharomyces 효모에서 ethyl acetate가, S. cerevisiae 효모는 ethanol 화합물이 높은 향기성분을 나타내었다.

• 한국식품영양과학회지
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• 제28권1호
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• pp.9-15
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• 1999

Taurine contents in alcoholic and non alcoholic beverages, milk and milk products, sugars and condiments were determined for 83 food items commonly used by Koreans. Taurine concentrations of food samples were analyzed by using an automated amino acid analyzer(Biochrom 20, Pharmacia LKB) based on ion exchange chromatography. Taurine was not detected in most carbonated beverages, but found in a variety of fruit and vegetable beverages(canned) in the range of 0.13 ~1.43mg taurine/ 100g wt, and in instant coffee or tea beverages(canned) in the range of 0.10~0.41mg taurine/100g wt. The traditional Korean turbid rice wine(takju) sample contained the highest level of taurine (2.29mg/100g wt) among alcoholic beverages tested, which was followed by wine(0.59~0.88mg tau rine/100g wt) and beer(0.53~0.73mg taurine/100g wt). Ordinary milk samples contained 1.05~1.40 mg taurine/100g wt, and a variety of taurine supplemented infant formulas manufactured by Korean companies included 17.3~25.4mg taurine/100g wt. Taurine was not detected in most sugars and sweetners except a couple of chocolate samples(2.13~2.18mg taurine/100g wt). Exceptionally high level of taurine was found in a curry powder sample(16.9mg taurine/100g wt), while the rest of commonly used condiments contained less than 2.90mg taurine/100g wt.

• Mycobiology
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• 제41권3호
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• pp.139-144
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• 2013

Makgeolli is a traditional cloudy-white Korean rice wine with an alcohol content of 6~7%. The present study investigated the morphological characteristics, carbon-utilizing ability, fatty acid composition, alcohol resistance, glucose tolerance, and flocculence of Saccharomyces cerevisiae Y98-5 and Pichia anomala Y197-13, non-S. cerevisiae isolated from Nuruk, which is used in brewing Makgeolli. Similar morphological characteristics were observed for both isolated wild yeast strains; and the carbon source assimilation of Y197-13 differed from that of other P. anomala strains. Strain Y197-13 was negative for D-trehalose, mannitol, arbutin, I-erythritol, and succinic acid. The major cellular fatty acids of strain Y197-13 included C18:2n6c (33.94%), C18:1n9c (26.97%) and C16:0 (20.57%). Strain Y197-13 was Crabtree-negative, with 60% cell viability at 12% (v/v) ethanol. The flocculation level of strain Y197-13 was 8.38%, resulting in its classification as a non-flocculent yeast.

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1749-1757
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• 2012

Makgeolli is a traditional rice wine favored by the general public in Korea. This study investigated the fermentation and sensory characteristics of using wild yeast strains for brewing makgeolli. A non-Saccharomyces cerevisiae strain was isolated from nuruk and termed Y197-13. It showed 98% similarity to Pichia anomala and had an optimal growth temperature of $25^{\circ}C$. Makgeolli was manufactured using koji, jinju nuruk, and improved nuruk as fermentation agents. Y197-13 makgeolli brewed with koji had alcohol and solids contents of 11.1% and 13.9%, respectively. Sweet sensory characteristics were attributed to residual sugars in makgeolli with 6% alcohol. The makgeolli had a fresh sour taste and carbonated taste. Volatile component analysis showed the isoamyl alcohol, phenylethyl alcohol, isoamyl acetate, and fatty acid, including ethyl oleate and ethyl linoleate, relative peak area was higher in Y197-13 makgeolli than in makgeolli with Saccharomyces cerevisiae. These results suggest the wild yeast, Y197-13, as a candidate for brewing makgeolli.

• Food Science and Biotechnology
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• 제16권6호
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• pp.975-980
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• 2007

Determination of ethyl carbamate content in imported alcoholic beverages in Korea and an exposure assessment were conducted. In gas chromatography/mass spectrometry/selected ion monitoring (GC/MS/SIM) analysis, 2.5-39, 8-263, 6.3-112, 11.3-23.5, 53-94, 8.5-38.5, 7-9.5, 21.3-31.5, 5-832.5, and $10.5-364.8\;{\mu}g/L$ of ethyl carbamate were detected in imported beers, sakes, whiskies, vodkas, Chinese liquors, cognacs, tequilas, rums, liqueurs, and wines, respectively. The exposure assessment indicated that the exposure of Korean adults to ethyl carbamate were lower than 20 ng/kg BW per day, (the virtual safe dose) indicating that the amount of ethyl carbamate exposed through fermented food and alcoholic beverages including imported products are currently in the 'no significant risk level'. However, the present low exposure to ethyl carbamate through the imported alcoholic products was not due to the low contents of ethyl carbamate in imported products, but low consumption of the imported products. Therefore, given increasing importation of alcoholic beverages in Korea, reductions of ethyl carbamate content in imported alcoholic beverages, especially non-distilled products, should be required by regulating limits on the ethyl carbamate content in the imported alcoholic beverages.

• 한국조리학회지
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• 제6권3호
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• pp.425-442
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• 2000

The main purpose of this study is to propose that Beverage sales should be step-up in Family Restaurant. To achieve the purpose of this study theoretical and empirical approaches were used. In review of theoretical background, basic concepts and characteristics of beverage were studied. For empirical study, three Family Restaurant in Korea was selected and analyzed the beverage sales which classified to Liquor, Beer Wine and Non-alcoholic beverage. Based on it, I suggest a plan of beverage sales step-up of Family Restaurant as follow. First, keep the appropriate valance of the whole item: non-alcohol and alcohol item. Second, develop the menu should be effect beverage sales. Third, through the promotion and event the restaurant should derive the customer to purchasing behavior. Fourth. server can accommodate and offer the information of food and beverage menu that can evoke the customer's attention.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.519-524
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• 2011

The correlation between alcoholic fermentation rate, measured as carbon dioxide ($CO_2$) evolution, and the rate of hydrogen sulfide ($H_2S$) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace $H_2S$ were directly impacted by yeast assimilable nitrogenous compounds in the grape juice. A series of model fermentations was conducted in temperature-controlled and stirred fermentors using a complex model juice with defined concentrations of ammonium ions and/or amino acids. The fermentation rate was measured indirectly by noting the weight loss of the fermentor; $H_2S$ was quantitatively trapped in realtime using a pre-calibrated $H_2S$ detection tube which was inserted into a fermentor gas relief port. Evolution rates for $CO_2$ and $H_2S$ as well as the relative ratios between them were calculated. These fermentations confirmed that total sulfide formation was strongly yeast strain-dependent, and high concentrations of yeast assimilable nitrogen did not necessarily protect against elevated $H_2S$ formation. High initial concentrations of ammonium ions via addition of diammonium phosphate (DAP) caused a higher evolution of $H_2S$ when compared with a non-supplemented but nondeficient juice. It was observed that the excess availability of a certain yeast assimilable amino acid, arginine, could result in a more sustained $CO_2$ production rate throughout the wine fermentation. The contribution of yeast assimilable amino acids from conventional commercial yeast foods to lowering of the $H_2S$ formation was marginal.

• 농업과학연구
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• 제48권2호
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• pp.299-310
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• 2021

Glycerol is a non-volatile compound with no aromatic properties that contributes significantly to the quality of wine by providing sweetness and richness of taste. In addition, it is also the third most significant byproduct of alcoholic fermentation in terms of quantity after ethanol and carbon dioxide. In this study, Fourier transform infrared (FT-IR) spectroscopy was employed as a fast non-destructive method in conjugation with multivariate regression analysis to build a model for the quantitative analysis of glycerol concentration in wine samples. The samples were prepared by using three varieties of red wine samples (i.e., Shiraz, Merlot, and Barbaresco) that were adulterated with glycerol in concentration ranges from 0.1 to 15% (v·v^-1), and subjected to analysis together with pure wine samples. A net analyte signal (NAS)-based methodology, called hybrid linear analysis in the literature (HLA/GO), was applied for predicting glycerol concentrations in the collected FT-IR spectral data. Calibration and validation sets were designed to evaluate the performance of the multivariate method. The obtained results exhibited a high coefficient of determination (R²) of 0.987 and a low root mean square error (RMSE) of 0.563% for the calibration set, and a R² of 0.984 and a RMSE of 0.626% for the validation set. Further, the model was validated in terms of sensitivity, selectivity, and limits of detection and quantification, and the results confirmed that this model can be used in most applications, as well as for quality assurance.