• Journal of the Korea Organic Resources Recycling Association
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• v.28 no.1
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• pp.53-64
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• 2020

A 2㎥/d combined wastewater treatment pilot plant containing the multi-stage vertical stacking type nitrification reactor was installed and operated for more than 1 year under the operating conditions of the short-circuit nitrogen process (pH 8, DO 1mg/L and Internal return rate 4Q from nitrification to denitrification reactor). For economically the combination treatment of food wastewater and the leachate from a landfill, the optimal combination ratio was operated by adjusting the food wastewater with the minimum oil content to 5-25% of the total throughput. The main treatment efficiency of the three-phase centrifugal separator which was introduced to effectively separate solids and oil from the food wastewater was about 52% of SS from 116,000mg/L to 55,700mg/L, and about 48% of normal hexane (NH) from 53,200mg to 27,800 mg/L. During the operational period, the average removal efficiency in the combined wastewater treatment process of BOD was 99.3%, CODcr 94.2%, CODmn 90%, SS 70.1%, T-N 85.8%, and T-P 99.2%. The average concentrations of BOD, CODcr, T-N, and T-P of the treated water were all satisfied with the discharge quality standard for landfill leachate ("Na" region), and SS was satisfied after applying the membrane process. On-site leachate had a relatively high nitrite nitrogen content in the combined wastewater due to intermittent aeration of the equalization tanks and different monthly discharges. Nevertheless nitrite nitrogen was accumulated, denitrification from nitrite nitrogen was observed rather than denitrification after complete nitrification. The average input of anti-forming chemical during the operation period is about 2L/d, which seems to be economical compared to the input of methanol required to treat the same wastewater.

• Korean Journal of Environmental Agriculture
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• v.18 no.2
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• pp.191-195
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• 1999

An integrated wastewater treatment pond system is developed for treatment and recycling of excreta from dairy cattle. It is composed of three ponds in series. A pit with a capacity of $10m^3$, 2-day hydraulic residence time, and overflow velocity of $1.5m^3m^{-2}day^{-1}$ is located internally in primary pond. It is designed for efficient sludge sedimentation and effective methane fermentation. It receives $5m^3/day$ of diluted cattle excreta by the water used for clearing stalls. A submerged gays collector for the recovery of methane is installed on the top of the pit. The average BOD_5 concentration of influent is 398.7mg/l. That of the effluent from primary pond is 49.2mg/l. About 88% of BOD_5 are removed in primary pond. It is assumed that about 60% of the influent BOD_5 is removed in the pit and that almost all of the carbon of the removed BOD_5 in the pit is converted to methane and carbon dioxide. Methane fermentation of the pit is well established at $16^{\circ}C$. This phenomena results from temperature stability, complete anaerobic condition, and neutral pH of the pit. Gas from the collector is almost 90% methane, less than 9% nitrogen, and less than 1% carbon dioxide. Thus a purified methane is produced, which can be used as energy source.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.630-638
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• 2019

Nitrous oxide (N₂O) is a greenhouse gas with a global warming potential 310 times higher than that of carbon dioxide. In this study, an N₂O-reducing consortium was obtained by enrichment culture using advanced treatment sludge as the inoculum. The dominant bacteria in the consortium were Sulfurovum (17.95%), Geobacter (14.63%), Rectinema (11.45%), and Chlorobium (8.24%). The consortium displayed optimal N₂O reducing activity when acetate was supplied as the carbon source at a carbon/nitrogen ratio (mol·mol^-1) of 6.3. The N₂O reduction rate increased with increasing N₂O concentration at less than 3,000 ppm. Kinetic analysis revealed that the maximum N₂O reduction rate of the consortium was 163.9 ㎍-N·g-VSS^-1·h^-1. Genes present in the consortium included nosZ (reduction of nitrous oxide to N₂), narG (reduction of nitrate to nitrite), nirK (reduction of nitrite to nitric oxide), and norB (reduction of nitric oxide to nitrous oxide). These results indicate that the N₂O-reducing consortium is a promising bioresource that can be used in denitrification and N₂O mitigation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.17-25
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• 2016

In this study, the air quality of underground and overground railway stations was evaluated focusing on the degree of influence of the outside air quality. The measured components were particulate matter ($PM_{10}$), carbon dioxide ($CO_2$), carbon monoxide (CO), nitrogen dioxide ($NO_2$), formaldehyde (HCHO), ozone ($O_3$), total airborne bacteria (TAB), total volatile organic carbon (TVOC), and Radon (Rn), which are included in the maintenance standards and recommended standards of the Indoor Air Quality Management Act. Also, the indoor/outdoor concentration ratios of $PM_{10}$, $NO_2$, and $O_3$ were calculated to estimate the influence of the outdoor air quality. The concentrations of $PM_{10}$ HCHO, TVOC, $NO_2$, and Rn in the underground stations were found to be higher than those in the overground stations. These results indicate that the (present) generation of contaminants are caused by the indoor source of the underground station. The ozone concentration of the overground stations was higher than that of the underground stations, which indicates that the outdoor ozone concentration influenced that of the overground stations directly. Thus, methods of improving the IAQ should take into consideration the types of contamination.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.335-340
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• 2010

This study was conducted to select organic materials (OM) and nitrogen sources in composting of waste mushroom bed from Agaricus bisporus. We examined physio-chemical properties of the organic materials and the mixture ratio for preparing the wasted mushroom bed (M) compost. The carbon content of sawdust was higher than those of rice straw (R) as OM source and the nitrogen content was high in the order of fowl manure (F)>> pig manure (P)> cow manure (C). The compost was prepared to maintain the criteria of above 25% organic matter and then the change of their ingredients was estimated during the process of fermentation. The temperature of waste mushroom bed+pig manure+rice straw (MRP) treatment was varied fast throughout fermentation, on the other hand the temperature of waste mushroom bed+pig manure+sawdust (MSP) treatment was steadily elevated to the middle of composting. The pH of the compost was somewhat high to pH 8.5~9.0 at the early stage, but decreased to 7.5 at the end stage of composting. The content of OM after fermentation was decreased to the level of 19~21% in rice straw, but the sawdust treatment maintained 25~27% organic matter. The waste mushroom bed+fowl manure+rice straw (MRF) treatment, which contains 26.2% organic matter and 0.68% nitrogen, was the highest among them. The volume of compost was reduced to 50% by using rice straw as organic matter, but reduced to 30% by using the sawdust. The contents of heavy metal in the compost were suitable within the legal criteria. The number of microorganisms were higher in the rice straw than those in the sawdust. It was high in the order of fowl manure> pig manure> cow manure. The major groups consisted of aerobic bacteria, gram negative bacteria and Bacillus sp. and their populations after fermentation were increased to $1{\times}10^1{\sim}1{\times}10^2\;cfu\;g^{-1}$ rather than those before fermentation. Therefore we concluded that the waste mushroom bed+fowl manure+sawdust (MSF 3:9:1 v/v/v) treatment was suitable combination for high organic matter and nitrogen source, and the periods of composting were 50~60 days.

• Journal of Korean Society on Water Environment
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• v.28 no.2
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• pp.255-268
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• 2012

Nonpoint source pollution has become a concern for water quality in the Han River system, especially during the high runoff events during the monsoon season. The patterns in nonpoint source runoff the relationships with land use, rainfall intensity, and stream nutrients concentrations were surveyed in 19 streams in the Han River watershed. The results show that the magnitude of NPS inputs of nutrients and sediment in the Han River watershed are of a serious concern. In the South Han River watershed, event mean concentrations (EMC) for biochemical oxygen demand (BOD), suspended sediment (SS), dissolved organic carbon (DOC), dissolved total phosphorus (DTP), total nitrogen (TN) Nitrate ($NO_3$-N) and total phosphorus (TP) were $1.94mg{\cdot}L^{-1},\;251mg{\cdot}L^{-1},\;2.75mg{\cdot}L^{-1},\;0.076mg{\cdot}L^{-1},\;2.82mg{\cdot}L^{-1},\;2.40mg{\cdot}L^{-1}$ and $0.232mg{\cdot}L^{-1}$, respectively. In the North Han River watershed, EMCs for BOD, SS, DOC, DTP, TN, $NO_3$-N and TP were $1.34mg{\cdot}L^{-1},\;172mg{\cdot}L^{-1},\;2.63mg{\cdot}L^{-1},\;0.032mg{\cdot}L^{-1},\;1.97mg{\cdot}L^{-1},\;1.55mg{\cdot}L^{-1}$ and $0.148mg{\cdot}L^{-1}$, respectively. The specific export coefficients of nutrient and sediments were much higher than those of other reports. Our study also found that the proportion of agricultural field area was significantly correlated with the EMCs for nutrients. Therefore, efforts to reduce NPS loading must focus on agricultural practices in the watershed. The relationships between land use and nutrient and sediment export found in this study can be used to derive estimates of runoff coefficients for agricultural field and as input data for modeling works and to develop total maximum daily load and best management practices in the Han River watershed.

• KSBB Journal
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• v.15 no.6
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• pp.621-629
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• 2000

A marine bacterium producing carotenoid was isolated from the Yosu coastal area of South Korea, and has been recorded as MCPBK-1. It was identified as Curtobacterium sp.. The optimum conditions of marine carotenoid fermentation from Cutobacterium sp. were pH 7.0, a temperature of $25^{\circ}C$, 4 mM fructose as a carbon source, 0.07% tryptone as a nitrogen source, 0.5 mM $M^{+2}$ ion as a mineral source and $1{\;}\mu\textrm{M}$ of cyanocobalamine as a growth factor in a $7{\;}\ell$ jar-fermentor. 13.0 mg/ml of the marine carotenoid were produced under optimum conditions. The crude marine carotenoid isolated was composed of 5 different compounds, i.e : tunaxanthin(86.6%), diatoxanthin (7.1%), ${\beta}-carotene$ (2.1%), canthaxanthin(1.9%) and cynthiaxanthin (1.9%). Physiological properties including antibacterial activity, cytotoxic effect, antioxidative effect and free radical scavenging activity were characterized with the crude carotenoid, which exhibited no antibacterial activity against E. coli and Lactobacillus bulgaricus, but a strong cytotoxic effect against cancer cells such as HepG2 (Hepatocellular carcinoma, human, ATCC HB-8065) and HeLa (Cervical carcinoma, human, ATCC CCL-2) cells, the ratios of impediment were 86.4% and 39.2%, respectively. This carotenoid, also, expressed a strong antioxidative effect (83%) against CCL-13 (diploid, monotypic hepatocyte, human, ATCC CCL-13) and exhibited free radical scavenging activity (43.4%) when using at a concentration of $50{\;}\mu\textrm{g}/ml$ of the crude carotenoid.

• Journal of Life Science
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• v.29 no.10
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• pp.1126-1135
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• 2019

From a sample of bamboo byproduct, the protease-producing yeast strain CO-1 was newly isolated. Strain CO-1 is spherical to ovoid in shape and measures $3.1-4.0{\times}3.8-4.4{\mu}m$. For the growth of strain CO-1, the optimal temperature and initial pH were $30^{\circ}C$ and 4.0, respectively. The strain was able to grow in 0.0-15.0%(w/v) NaCl and 0.0-9.0%(v/v) ethanol. Based on a phylogenetic analysis of its 18S rDNA sequences, strain CO-1 was identified as Pichia anomala. The extracellular protease produced by P. anomala CO-1 was partially purified by ammonium sulfate precipitation, which resulted in a 14.6-fold purification and a yield of 7.2%. The molecular mass of the protease was recorded as approximately 30 kDa via zymogram. The protease activity reached its maximum when 1.0%(w/v) CMC was used as the carbon source, 1.0%(w/v) yeast extract was used as the nitrogen source, and 0.3%(w/v) $MnSO_4$ was used as the mineral source. The protease revealed the highest activity at pH 7.0 and $30^{\circ}C$. This enzyme maintained more than 75% of its stability at a pH range of 4.0-10.0. After heating at $65^{\circ}C$ for 1 hr, the neutral protease registered at 60% of its original activity. The protease production coincided with growth and attained a maximal level during the post-exponential phase.

• Applied Biological Chemistry
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• v.48 no.3
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• pp.207-211
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• 2005

The metabolites released from cultures of rhizosphere bacteria can inhibit plant growth. Bacillus subtilis IJ-31 inhibited plant growth by the production of hydrocinnamic acid (HCA). The production of HCA by plant-growth inhibiting rhizobacterium B. subtilis IJ-31 was optimized. $90.5\;{\mu}g/ml$ of HCA was obtained under the condition of 1% rice bran as carbon source, 0.5% tryptone as nitrogen source, 0.1% $ZnCl_2$ as metal source at $37^{\circ}C$ for 60 h (pH 7.0). The optimal condition for the HCA production by B. subtilis IJ-31 in the jar fermenter was established using response surface methodology (RSM) of statistical analysis system(SAS) program. The production of HCA by B. subtilis IJ-31 in the jar fermenter culture reached $102.99\;{\mu}g/ml$ when 2.24% soil extracts was added and agitation speed was 290 rpm under the same condition. And the experimental value of HCA production is $102.5\;{\mu}g/ml$ in the same culture condition. The production of HCA by B. subtilis IJ-31 is higher as 12% than that from the flask culture.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.117-126
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• 2010

A chitinase (CHT) and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU017, with shrimp shell as the sole carbon/nitrogen source. The molecular masses of CHT and PRO determined by SDS-PAGE were approximately 65 kDa and 53 kDa, respectively. CHT was inhibited by $Mn^{2+}$ and $Cu^{2+}$, and PRO was inhibited by most tested divalent metals and EDTA. The optimum pH, optimum temperature, pH stability, and thermal stability of CHT and PRO were pH 5, $50^{\circ}C$, pH 5-7, and <$50^{\circ}C$, and pH 9, $40^{\circ}C$, pH 5-11, and <$40^{\circ}C$, respectively. PRO retained 95% of its protease activity in the presence of 0.5 mM SDS. The result demonstrates that PRO is an SDS-resistant protease and probably has a rigid structure. The $4^{th}$-day supernatant showed the strongest antioxidant activity (70%, DPPH scavenging ability) and the highest total phenolic content ($196{\pm}6.2\;{\mu}g$ of gallic acid equiv./ml). Significant associations between the antioxidant potency and the total phenolic content, as well as between the antioxidant potency and free amino groups, were found for the supernatant. With this method, we have shown that shrimp shell wastes can be utilized and it is effective in the production of enzymes and antioxidants, facilitating its potential use in industrial applications and functional foods.