• 약학회지
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• 제54권5호
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• pp.387-391
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• 2010

Polygonum cuspidatum which is widely distributed in Korea has been used as treatments of dermatitis, gonorrhea and inflammation in traditional medicine. We examined anti-inflammatory activities by the inhibition of NO production in RAW264.7 murine macrophages cells. Phytochemical examination of Polygonum cuspidatum led to the isolation and characterization of emodin (1), emodin 8-O-${\beta}$-D-glucopyranoside (2), emodin 1-O-${\beta}$-D-glucopyranoside (3). Antioxidative activities of these compounds were determined by measuring the radical scavenging effects on DPPH radicals. Compounds 1 and 3 showed potent activities compared with L-NMMA. These results suggested that the antraquinone compounds isolated from Polygonum cuspidatum might be used as antiinflammatory agents.

• 한방안이비인후피부과학회지
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• 제21권3호
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• pp.104-110
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• 2008

Objectives : This experiment study was performed to investigate the anti-inflammation and anti-oxidant effects of Indigo Naturalis(IN) and Rehmanniae Radix(RR) which were herbs for clearing heat. Methods : Anti-inflammation and anti-oxidation effects of IN and RR that were measured by the inhibitory ability of Nitric oxide(NO) production and the scavenging for 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Results : 1. IN and RR groups were not showed cell toxicity. 2. In the inhibitory ability of NO production, IN groups were better than RR groups, but there were no statistical significances among the groups. 3. In the scavenging for DPPH radical, IN groups were better than RR groups, but there were no statistical significances among the groups. Conclusions : These results showed that the IN group was better than the RR group in treating skin eruptions, especially those such as erythema, purple spot, papule, pustules which appear in acute inflammation.

• 대한화장품학회지
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• 제46권4호
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• pp.329-337
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• 2020

본 연구는 willamette 품종의 동결건조라즈베리분말, n-hexane 및 ethyl acetate를 이용하여 라즈베리 추출물(Rubus idaeus (Raspberry) fruit extract, RIFE)을 제조한 다음 페놀 화합물 함량, 철 이온 환원능력, 그리고 라디칼 소거 능력의 측정을 수행하였다. Willamette 품종 라즈베리추출물은 10% 농도까지 세포독성을 보이지 않았다. 해당 농도로 실험을 수행한 결과 총 페놀 화합물 함량은 375.3 ppm, 총 플라보노이드 함량은 43.46 ppm이 포함된 것을 확인하였고, ferric reducing antioxidant power (FRAP) reagent를 이용한 철 이온 환원능력은 FeSO4 0.532 mM에 해당하는 환원능력을 가졌음을 확인하였다. 이를 바탕으로 항산화 소재의 대표적 원료인 L-ascorbic acid와 비교하는 라디칼 소거능 실험을 실시하였다. 그 결과 1,1-diphenyl- 2-picrylhydrazyl (DPPH) 라디칼 소거능력은 94.5 ± 0.7%를 나타냈고, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) 라디칼 소거능은 99.4 ± 2.82%를 나타냈으며, nitric oxide (NO) 라디칼소거능은 88.5 ± 0.4%를 나타냈다. 표준인 L-ascorbic acid 용액과 비교할 때 25 - 50 ppm 사이의 효능을 지닌 DPPH 라디칼 소거능 / 100 ppm에 근접한 효능을 지닌 ABTS 라디칼 소거능 / 1,000 ppm 이상의 효능을 지닌 NO 라디칼 소거능을 가졌음을 확인하였다. 이러한 결과들은 willamette 품종 라즈베리추출물이 항산화 활성을 가지는 효과적인 화장품 소재로 적용 가능하다는 것을 시사한다.

• 생약학회지
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• 제42권2호
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• pp.195-200
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• 2011

In this study, we investigated on antioxidative activity, tyrosinase inhibitory activity and nitric oxide(NO) production inhibitory activity in the hot water extract of Taraxacum coreanum and its fractions. The total polyphenol and flavonoid contents of the extract were found to be 65.42 mg/g and 9.83 mg/g, respectively. And the total polyphenol and flavonoid contents of the ethyl acetate fraction were found to be 168.23 mg/g and 31.92 mg/g, respectively. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate and butanol fraction were exhibited 64.65 ${\mu}g/ml$ and 277.42 ${\mu}g/ml$, respectively. Moreover, ethyl acetate fraction showed higher inhibitory activity than other samples in tyrosinase inhibitory activity. In NO production inhibitory activity, the extract and its fractions showed NO production inhibitory effect. Especially, the ethyl acetate, chloroform and butanol fraction was exhibited higher NO production inhibitory activity than other samples. As a result, the ethyl acetate and butanol fraction from the water extract of T. coreanum could be applicable to functional materials for skin-related fields.

• 대한화장품학회지
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• 제44권3호
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• pp.335-341
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• 2018

본 연구는 버드나무 속 식물의 화장품 소재로써의 활용 가능성을 평가하기 위해 수행하였다. 버드나무 속 식물 중 갯버들, 능수버들 및 키버들 70% 에탄올 추출물의 DPPH radical 소거능 분석을 통한 항산화 활성을 평가한 결과 모든 추출물이 대조군에 비해 DPPH radical 소거능이 유의적으로 증가하였다. 또한 3종의 버드나무 속 식물 추출물 처리는 RAW 264.7 세포의 nitric oxide (NO) 생성을 유의적으로 억제하여 항염 활성이 있는 것으로 확인되었으며, 모두 콜라게나제 저해 활성이 있는 것으로 확인되었다. 그 중 갯버들 추출물이 가장 높은 콜라게나제 저해활성을 나타내어 갯버들 추출물의 용매 분획물에 대한 추가적인 콜라게나제 저해활성을 분석한 결과, 물 및 부탄올 분획물이 가장 높은 콜라게나제 저해활성이 있는 것으로 확인되었다. 결과적으로 버드나무 속 식물 중 갯버들은 높은 콜라게나제 저해활성이 있는 것으로 확인되어 향후 효과적인 기능성 화장품 소재로 활용 가능 할 것으로 사료된다.

• 한국약용작물학회지
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• 제27권4호
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• pp.259-270
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• 2019

Background: Eucommia ulmoides has long been used as an herbal medicine for the treatment of diabetes, hypertension and other diseases in many Asian countries. Methods and Results: This study aimed at evaluating the antioxidant and anti-inflammatory properties of its water (EU-DW, and REU-DW) and ethanol (EU-EtOH, and REU-EtOH) extracts, as well as those of non-roasted E. ulmoides (EU) and roasted EU (REU) cortex. The following were assessed in each extract: total phenolic and flavonoid contents, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and nitrite radical scavenging activities, reducing power, DNA damage prevention activity, and nitric oxide (NO) inhibition activity. Both EU and REU extracts showed high phenolic and flavonoid content, dose-dependent DPPH radical scavenging capacity, high reducing activity, and considerable DNA damage prevention activity. EU extracts showed remarkable ABTS free radicals scavenging capacity. REU extracts showed a higher radical scavenging capacity and played an important role in inhibiting NO production. Conclusions: The results of this study suggested that aqueous and ethanol extracts of EU and REU possess antioxidant capacities, and prevent oxidative damage to DNA, probably owing to their phenolic and flavonoid content. Therefore, EU and REU could be candidates antioxidant supplements.

• 대한한방내과학회지
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• 제29권2호
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• pp.421-431
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• 2008

Objectives : This study was to investigate the inhibitory effects of Ulmus davidiana on the generation of peroxynitrite $(ONOO^{-})$, nitric oxide (NO) and superoxide anion radicals $(O_{2}^{-})$ in the endothelial cells of rat vessels. The effects of Ulmus davidiana on the expression of inflammation-related proteins, $NF-{\kappa}B$ (p50, p65), COX-2, and iNOS, were examined by western blotting. Methods : For this study, fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed via using anti-$NF-{\kappa}B$ (p50, p65), anti-COX-2, and anti-iNOS, respectively. Results : Ulmus davidiana inhibited the generation of $ONOO^{-}$, NO and $(O_{2}^{-})$ in the lipopolysaccharide (LPS)-treated endothelial cells of rat vessels in vitro. Ulmus davidiana inhibited the expression of COX-2 and iNOS genes by means of decreasing the $NF-{\kappa}B$ activation. Conclusions : These results suggest Ulmus davidiana is effective on inhibiting the generation of $ONOO^{-}$, NO and $O_{2}^{-}$, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.

• 대한의생명과학회지
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• 제23권4호
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• pp.411-415
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• 2017

To evaluate the protective effects of Plantago Major extract (PME) in stimulated BV-2 microglial cells and its anti-oxidant properties, cell viability assessment was performed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to activate BV-2 microglia. Nitric oxide (NO) levels were measured using Griess assay. Tumor necrosis factor-alpha (TNF-${\alpha}$) production was evaluated by enzyme-linked immunosorbent assay (ELISA). Antioxidant properties were evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. LPS-activated excessive release of NO in BV-2 cells was significantly inhibited by PME (P < 0.001 at $100{\mu}g/mL$). PME also scavenged DPPH radicals in a dose-dependent manner (P < 0.05 at $10{\mu}g/mL$ and P < 0.001 at $20{\sim}200{\mu}g/mL$). These results indicate that PME attenuated neuroinflammatory responses in LPS-activated BV-2 microglia by inhibiting excessive production of pro-inflammatory mediators such as NO and TNF-${\alpha}$. The anti-neuroinflammatory potential of PME may be related to its strong antioxidant properties.

• Advances in Traditional Medicine
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• 제7권3호
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• pp.217-228
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• 2007

Agrimonia pilosa Ledeb. has long been used for a useful natural agent ameliorating inflammation related symptoms in the folk medicine recipe. This study was performed to investigate effects of Agrimonia pilosa Ledeb.(AP) on the expression of inflammation related genes such as the inducible nitric oxide synthase (iNOS) in macrophage cell line, RAW 264.7 cells. The AP (whole plants) was extracted with 80% ethanol and sequentially partitioned with solvents in order to increase polarity. Among the various solvent extracts of AP, the n-butanol (BuOH) fraction showed the most powerful inhibitory ability against nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells without affecting cell viability. Reverse transcriptase-polymerase chain reaction and Western blot analysis revealed that the BuOH fraction provided a primary inhibitor of the iNOS protein and mRNA expression in LPS-induced RAW 264.7 cells. The DPPH and OH radical scavenging activities of the several fractions of 80% ethanol extracts of AP significantly increased by EtOAC and BuOH fractions. Thus, the present study suggests that the response of a component of the BuOH fraction to NO generation via iNOS expression provide an important clue to elucidate anti-inflammatory mechanism of AP.

• 생약학회지
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• 제40권1호
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• pp.77-81
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• 2009

Abies nephrolepis(Pinaceae) extracts were tested for determined immune system regulating activity based on antiinflammatory activity, antioxidant activity and anti-proliferative effect on HeLa cell line. The A. nephrolepis extracts increased dose-dependently anti-proliferation of HeLa cell line. The DM fraction of the extracts having anti-proliferatative effects of HeLa cell line was fractionalized four subfractions($D1{\sim}D4$). Inflammation-induced NO production was inhibited by D2 and D4 in LPS-activated RAW264.7 macrophages. And also, this fractions showed antioxidant activity examined by DPPH radical scavenging effects. These results suggest that the potential use of DM fraction of A. nephrolepis in chemoprevention and regulation overproduction of NO on pathogenic conditions. The mechanism of the inflammatory effects, however, must be evaluated through various parameters in the induction cascade of NO production.