Browse > Article

Inhibitory Effect on the Lipopolysaccharide-Induced Nitric Oxide Formation in RAW264.7 and on the Proliferation of HeLa Cell Lines by the Stem Bark Extracts of Abies nephrolepis  

Bae, Ki-Eun (Department of Forest product, Kook min University)
Chong, Han-Soo (Department of Forest product, Kook min University)
Kim, Dong-Sup (Department of Forest product, Kook min University)
Choi, Young-Woong (Department of Forest product, Kook min University)
Kim, Yeung-Suk (Department of Forest product, Kook min University)
Kim, Young-Kyoon (Department of Forest product, Kook min University)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.1, 2009 , pp. 77-81 More about this Journal
Abstract
Abies nephrolepis(Pinaceae) extracts were tested for determined immune system regulating activity based on antiinflammatory activity, antioxidant activity and anti-proliferative effect on HeLa cell line. The A. nephrolepis extracts increased dose-dependently anti-proliferation of HeLa cell line. The DM fraction of the extracts having anti-proliferatative effects of HeLa cell line was fractionalized four subfractions($D1{\sim}D4$). Inflammation-induced NO production was inhibited by D2 and D4 in LPS-activated RAW264.7 macrophages. And also, this fractions showed antioxidant activity examined by DPPH radical scavenging effects. These results suggest that the potential use of DM fraction of A. nephrolepis in chemoprevention and regulation overproduction of NO on pathogenic conditions. The mechanism of the inflammatory effects, however, must be evaluated through various parameters in the induction cascade of NO production.
Keywords
Abies nephrolepis; Pinaceae; Antioxidant; Antiinflammation; Anticancer;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 MukIS, N., Ishikawa, Y., Ikeda, N., Fujioka, N., Watanabe, S. and Kuno, K. (1996) Novel insight into molecular mechanism of endotoxin shock; biochemical analysis of LPS receptor signaling in a cell-free system targeting NF-kapperB and regulation of cytokine production/action through beta2 integrin in vivo. J. Leukoc. Biol. 59: 145-151   PUBMED   ScienceOn
2 Richard, G. and Salvador, M. (1994) Nitric oxide synthases in mammals. Biochem. J. 298: 249-258   DOI   PUBMED
3 Fabio, V., Kobuchi, H. and Lester, P. (1998) Procyanidins extracted from Pinus maritima (Pycnogenol): scavengers of free radical species and modulators of nitrogen monoxide metabolism in activated murine RAW 264.7 macrophages. Free Radic Biol Med. May: 24(7-8): 1120-9   DOI   ScienceOn
4 Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. Journal of Immunological Methods. 65: 55-63   DOI   PUBMED   ScienceOn
5 Lee, E. S., Ju, H. K., Moon, T. C., Lee, E., Jahng, Y., Lee S.H., Son, J. K., Baek, S. H. and Chang, H. W. (2004) Inhibition of nitric oxide and tumor necrosis factor-$\alpha$ (TNF-$\alpha$) production by propenone compound through blockade of nuclear factor (NF)-$\kappa$B activation in cultured murine macrophage. Biol. Pharm. Bull. 27: 617-620   DOI   ScienceOn
6 Funk, C. D., Frunk, L. B., Kennedy, M. E., Pong, A. S. and Fitzgerald, G. A. (1991) Human platelet/erythroleukemia cell prostaglandin G/H synthase: cDNA cloning, expression, and gene chromosomal assignment. FASEB J. 5: 2304-2312   DOI   PUBMED   ScienceOn
7 Vane, J. A. (1971) Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like durgs. Nat. New. Biol. 23: 232-235   DOI   ScienceOn
8 Lee, S. J., Lee, K. W., Lee, H. J. (2004) Abies nephrolepis leaf phenolics prevent the inhibition of gap junction intercellular communication by hydrogen peroxide in rat liver epithelial cells. Biofactors. 21(1-4): 357-60   DOI   ScienceOn
9 Joydeb, K. K., Young, J. S. (2008) Inflammation: Gearing the journey to cancer. Mutation Research. 659: 15-30   DOI   ScienceOn
10 Yokozawa, T., Chen, D. P., Kong, E., Tanaka, T., Nonaka, G.I. and Nishioka, I. (1998) Study on the inhibitory effect of tanins and flavonoids against the 1,1-diphenyl1-2 picrylhydrazyl radical. Biochem Pharmacol. 56: 213-222   DOI   ScienceOn
11 이창복 (1999) 대한식물도감, 233 향문사, 서울
12 Pharham, P. (2005) The Immune System. Garland Science, New York
13 Balkwill F., Mantovani, A. (2001) Inflammation and cancer: back to Virchow? Lancet. 357: 537-45   DOI   ScienceOn
14 Kim, S. K., Jung, S. M., Ahn, K. H., Jeon, H. J., Lee, D. H., Jung, K. M., Jung, S. Y. and Kim, D. K. (2005) Identification of three competitive inhibitors for membrane-associated, $Mg^{2+}$-dependent and neutral 60 $\kappa$Da sphingomyelinase activity. Arch Pharm Res. Aug: 28(8): 923-9   DOI   ScienceOn
15 McCord, J. (1974) Free radicals and inflammation: protection of synorial fluid by superoxide dismutase. Science. 185: 529-531   DOI   PUBMED   ScienceOn