• Journal of Food Hygiene and Safety
• /
• v.31 no.4
• /
• pp.304-309
• /
• 2016

This study was assessed stability of ethanolic extract from Cirsium setidens Nakai that will be applied for development of functional foods and ingredients. We evaluated pectolinarin content, total phenol content and antioxidant activity (DPPH radical scavenging activity and FRAP assay) of ethanolic extract from C. setidens Nakai against various temperature (4, 25 and $50^{\circ}C$) and pH (4.0, 7.0 and 10.0). Our results show that the pectolinarin and total phenol contents in ethanolic extract from Cirsium setidens Nakai slightly reduced during the storage periods. Moreover, the DPPH radical scavenging activity at $4^{\circ}C$ was higher than those of other temperature. Pectolinarin content, total phenol content and DPPH radical scavenging activity of ethanolic extract from Cirsium setidens Nakai at acidic (pH 4.0) and neutral (pH 7.0) pH ranges were higher than at alkaline pH ranges. These results indicate that the optimum storage condition of C. setidens Nakai ethanol extract are temperature $4^{\circ}C$ and pH 4.0-7.0 ranges.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.27 no.2
• /
• pp.45-56
• /
• 2001

Korea mountain ginseng known as oriental miracle drug is an important medicinal plant. The effect of mountain ginseng adventitious roots extract has been described. The valuable root of mountain ginseng contained several kinds of ginsenosides that have been confirmed to have many active functions for the human body. However, the study of mountain ginseng has a limit because the price of wild ginseng is very expensive and rare. The mountain ginseng adventitious roots were derived from mountain ginseng callus that were induced from mountain ginseng roots. Adventitious roots were separated from callus and grown in solid media(Murachige and stoog media). It was cultured in a 20L bioreactor. After culturing for 40days, adventitious roots were harvested. Afterwards the harvested mountain ginseng adventitious roots were dryed and extracted. We examined the effect on melanogenesis of mountain ginseng adventitious roots extract. Here, we report the inhibitory effect of melanin biosynthesis on the adventitious roots extract of In vitro test. Also, we assessed the safety of adventitious roots extract. In vitro, cytotoxicity of adventitious roots extract was assessed in mouse fibroblast using two method: The neutral red uptake assay and the MTT assay. In vivo, the allergic and irritant were Patch teated in 30 patients. Consequently, extract of mountain ginseng adventitious roots have inhibitory effect on melanin biosynthesis in B-16 melanoma cell test, tyrosinase inhibitory test and DOPA auto-oxidation test. There were decreased 86%(0.5% concentration), 45%(1% concentration) and 61%(1% concentration), respectively.

• Journal of the Korea Society of Computer and Information
• /
• v.18 no.9
• /
• pp.131-138
• /
• 2013

In this paper, I propose and evaluate the method that classifies emotional type of characters with their emotional words. Emotional types are classified as three types such as positive, negative and neutral. They are selected by classification of emotional words that characters speak. I propose the method to extract emotional words based on WordNet, and to represent as emotional vector. WordNet is thesaurus of network structure connected by hypernym, hyponym, synonym, antonym, and so on. Emotion word is extracted by calculating its emotional distance to each emotional category. The number of emotional category is 30. Therefore, emotional vector has 30 levels. When all emotional vectors of some character are accumulated, her/his emotion of a movie can be represented as a emotional vector. Also, thirty emotional categories can be classified as three elements of positive, negative, and neutral. As a result, emotion of some character can be represented by values of three elements. The proposed method was evaluated for 12 characters of four movies. Result of evaluation showed the accuracy of 75%.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.10
• /
• pp.1430-1433
• /
• 2004

A study was conducted to determine the effect of different alkali treatments on changes in chemical composition and on degradability of bagasse and rice straw. This study divided into 2 experiments, the first with bagasse and the second with rice straw. Each experiment comprised 9 treatments which included: untreated control; 3% NaOH; 6% NaOH; 3% urea; 6% urea; 3% NaOH/3% urea; 3% NaOH/6% urea; 6% NaOH/3% urea; 6% NaOH/6% urea. In both experiments, crude protein contents were increased from 2.0 to 12.5 units for bagasse and 3.1 to 13.7 units for rice straw by urea treatments. Ash contents of the treated bagasse and rice straw were increased over the untreated control (1.5-9.7 units for bagasse; 4.2-8.8 units for rice straw). The effects on ether extract, crude fiber, neutral detergent fiber and acid detergent fiber of the treated bagasse and rice straw were variable. Nylon bag degradability of dry matter and crude fiber were increased by treatments applying NaOH and NaOH plus urea but not urea alone. In contrast, the egradability of neutral detergent fiber and acid detergent fiber were reduced compared with the untreated control. From these degradability studies, it can be concluded that the most efficient treatments of bagasse were those treatments with 6% NaOH, followed by treatments with 6% NaOH plus 3% or 6% urea and 3% NaOH plus 3% or 6% urea, respectively. However, when comparison was made on the cost of chemical used to treat the agricultural by-products, particularly in case of rice straw, 3-6% urea would be appropriate.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.2
• /
• pp.188-199
• /
• 2015

Nineteen tanniferous browse plants were collected from South Africa to investigate their digestibility, gas production (GP) characteristics and methane production. Fresh samples were collected, dried in forced oven, and ground and analyzed for nutrient composition. In vitro GP and in vitro organic matter digestibility (IVOMD) were determined using rumen fluid collected, strained and anaerobically prepared. A semi-automated system was used to measure GP by incubating the sample in a shaking incubator at $39^{\circ}C$. There was significant (p<0.05) variation in chemical composition of studied browses. Crude protein (CP) content of the species ranged from 86.9 to 305.0 g/kg dry matter (DM). The neutral detergent fiber (NDF) ranged from 292.8 to 517.5 g/kg DM while acid detergent fiber (ADF) ranged from 273.3 to 495.1 g/kg DM. The ash, ether extract, non-fibrous carbohydrate, neutral detergent insoluble nitrogen, and acid detergent insoluble nitrogen and CP were negatively correlated with methane production. Methane production was positively correlated with NDF, ADF, cellulose and hemi-cellulose. Tannin decreased GP, IVOMD, total volatile fatty acid and methane production. The observed low methanogenic potential and substantial ammonia generation of some of the browses might be potentially useful as rumen manipulating agents. However, a systematic evaluation is needed to determine optimum levels of supplementation in a mixed diet in order to attain a maximal depressing effect on enteric $CH_4$ production with a minimal detrimental effect on rumen fermentation of poor quality roughage based diet.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.5
• /
• pp.693-701
• /
• 2003

In order to evaluate the nutritive value of the forage plants in South Sulawesi, Indonesia, 266 samples (61 grasses and 65 legumes grown in the dry season, 60 grasses and 80 legumes grown in the rainy season) were collected from the highland and lowland in 1998 to 2000, and were subjected to the determination of chemical composition and digestibility. The least-squares analysis of variance demonstrated that the in vitro dry matter digestibility (IVDMD) of grasses was not significantly affected by season or altitude. On the other hand, the some proximate components and cell wall components were significantly affected by season and altitude including the season${\times}$altitude interaction. For the legumes, the in vitro neutral detergent fiber digestibility (IVNDFD) and cellulose content were significantly affected by season. On the other hand, the ether extract (EE) content was significantly affected by season and altitude. The interaction of the season${\times}$altitude for IVDMD, of the year${\times}$season for some proximate components and of the year${\times}$season and the season${\times}$altitude for some cell wall components were significant. These results indicate that the forages grown at highland in dry season have a relatively high quality. The means of the total digestible nutrient (TDN) content estimated from IVDMD in grasses and in legumes were 50.3% and 57.4%, respectively, and the crude protein contents were 7.7% and 17.6%, respectively. The correlation coefficients between IVDMD and the contents of crude fiber, neutral detergent fiber and acid detergent fiber were relatively high in all of forage plants, suggesting that these components would provide an accurate prediction of digestibility or TDN content. A close relationship between IVNDFD and lignin content indicates that the lignin would be the most accurate predictor of cell wall digestibility.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.11
• /
• pp.1531-1539
• /
• 2009

This study compared the nutritional value of canola (B. napa) and mustard (B. hirta) press cakes obtained from the biodiesel industry as ingredients for use in diets fed to broiler chickens. A total of 210, one-day old, male broiler chicks were randomly assigned to one of seven dietary treatments. The control diet was based on wheat and soybean meal and contained 15% canola meal. For the experimental diets, 5, 10 or 15% of the canola meal was replaced with an equal amount of either canola or mustard biodiesel press cake. Dry matter and neutral detergent fiber digestibility were significantly higher for birds fed diets containing either canola or mustard biodiesel press cake compared with canola meal. Dry matter and neutral detergent fiber digestibility of the canola biodiesel press cakes was higher than the mustard biodiesel press cakes. Ether extract digestibility and nitrogen retention were significantly higher for birds fed canola biodiesel press cake compared with canola meal and mustard biodiesel press cake. Body weight gain and feed intake did not differ between birds fed canola or mustard biodiesel press cake and canola meal. In addition, there was no significant difference in body weight gain or feed intake between birds fed diets containing canola or mustard biodiesel press cake. Feed conversion was significantly improved for birds fed either canola or mustard biodiesel press cake compared with canola meal. Mortality was unaffected by treatment. Since the performance of broilers fed canola biodiesel press cakes was essentially the same as that of broilers fed canola meal, it is difficult to justify a premium to be paid for canola biodiesel press cake over that paid for canola meal. In addition, there was no difference in the performance of broilers fed biodiesel press cake obtained from canola or mustard seed. As mustard seeds are generally available at a lower price than canola seed, there may be some incentive to use mustard rather than canola seed for producing biodiesel press cake for use in poultry production.

• Journal of Korean Society on Water Environment
• /
• v.32 no.6
• /
• pp.628-648
• /
• 2016

Increased attention has been paid to the presence of veterinary antibiotics in various environmental matrices due to their toxicological behavior in the ecosystem and development of antibiotic-resistant strains of pathogenic bacteria. In the this review, 37 target antimicrobials were selected based on annual sales of antibiotics for livestock in South Korea 2014. Also, extraction and clean-up methods for the determination of the antibiotic residues in liquid samples including water, milk, and honey were comprehensively reviewed in the literature. Solid-phase extraction (SPE) was commonly used as a pre-treatment method for the samples. Most of the analytes were extracted in acidic conditions (2.5~4.0) except for aminoglycosides, which were extracted in neutral conditions (7.0~8.0). ${\beta}-Lactams$ showed the highest recoveries in neutral pH due to their degradation characteristics in acidic media. Starta-X, Oasis HLB, and Oasis MCX were frequently applied as an SPE cartridge and Oasis HLB showed the highest recoveries for the majority of antibiotic classes. The homogenized honey and milk were extracted by mixing with acids for deproteinization. Solids and other interfering substances in the extract were eliminated by centrifugation followed by membrane filtration or SPE before injection into HPLC.

• Journal of the Korean Wood Science and Technology
• /
• v.20 no.4
• /
• pp.73-84
• /
• 1992

It was observed that the hot-water extract of the bark of Kalopanax pictus N. had the highest anti-complementary activity among the 11 kinds of forest materials. Methanol-and ethanol-soluble portions had low anti-complementary activities, but crude polysaccharide. HKP-0 had a high activity of 80%. HKP-0 contained 54.8% of total sugar and 27.9% of protein. The neutral sugars of HKP-0 consisted of mainly arabinose, galactose and glucose. HKP-4 fraction obtained by cetavlon treatment of HKP-0 showed the highest anti-complementary activity of 90%. The activity was not changed by pronase digestion bu decreased greatly by periodate oxidation. HKP-4 consisted of mainly arabinose and glucose with molar ratio of 1.0 : 22.4, HKP-4-I, an unabsorbed fraction from HKP-4 on DEAE Sepharose CL-6B column showed higher yield and activity than those of absorbed fractions. HKP-4-I was homogeneous, and its molecular weight was about 25,000. HKP-4-I contained 84.0% of neutral sugar and consisted of arabinose and glucose with molar ratio of 1.0 : 11.2. The anti-complementary activity of HKP-4-I was not decreased by the treatment of polymyxin B, and the polysaccharide activated both classical and alternative pathway in complement system. Void volume fraction obtained from HKP-4-I hydrolyzed with ${\alpha}$-amylase on Sephadex G-25 column only had a high anti-complementary activity.

• Asian-Australasian Journal of Animal Sciences
• /
• v.25 no.6
• /
• pp.861-868
• /
• 2012

During two years, four samples per year were collected in Vietnam from rice bran, cassava residue, brewer's grain, tofu residue, soybean meal, coconut cake, sweet potato vines and water spinach for chemical analysis and assessment of water holding capacity (WHC). The selected feedstuffs represent fibre-rich plant sources and agro-industry co-products commonly used in pig feeding in Vietnam. The content (g/kg DM) of crude protein (CP), ether extract (EE) and non-starch polysaccharides (NSP) varied between feedstuffs and ranged from 21 to 506 for CP, from 14 to 118 for EE and from 197 to 572 for NSP. Cassava residue had a high starch content of 563 g/kg DM, while sweet potato vines, water spinach, coconut cake and soybean meal had a high content of sugars (63-71 g/kg DM). The content of individual neutral sugars varied between feed ingredients, with the highest content of arabinose, galactose and glucose in tofu residue, the highest content of xylose in brewer's grain and the highest content of mannose in coconut cake. The content of uronic acid was high for cassava residue, tofu residue, sweet potato vines and water spinach (57-88 g/kg DM). The content of soluble non-cellulosic polysaccharides (S-NCP) was positively correlated ($r^2$ = 0.82) to the WHC. The content (g/kg DM) of CP, NDF, neutral sugars, total NSP, total NCP, S-NCP and total dietary fibre in tofu residue, water spinach and coconut cake varied (p<0.05) between years. In conclusion, diet formulation to pigs can be improved if the variation in chemical composition of the fibre fraction and in WHC between potential feed ingredients is taken into account.