• Archives of Pharmacal Research
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• 제24권4호
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• pp.300-306
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• 2001

A novel and two known bioactive mono-tetrahydrofuran (THF) annonaceous acetogenins, annomocherin (1), annonacin (2) and annomontacin (3), have been isolated from the fractionated ethanolic extracts of the seeds of Annona cherimolia, guided by the brine shrimp lethality test (BST). Their structures were elucidated on the basis of spectroscopic and chemical methods. All compounds have a relative stereochemistry of threo/trans/threo for the mono-THF ring with two flanking hydroxyls. Compound 1 has a double bond at C-23/ 24 of aliphatic chain. Compound 1 was isolated from natural sources for the first time, and was named annomocherin. Two known Compounds 2 and 3 which have never been isolated from this species before, were obtained. Compound 1 exhibited potent and selective cytotoxicities against the breast carcinoma (MCF-7) and kidney carcinoma (A-498) cell lines with 100 to 1,000 times the potency of adriamycin. In brine shrimp lethality test (BST), 1-3 exhibited cytotoxicity.

• Natural Product Sciences
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• 제13권4호
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• pp.355-358
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• 2007

Further bioactivity-directed fractionation of the ethanol extract of the seeds of Annona cherimolia has led to the isolation of two mono-tetrahydrofuran acetogenins, xylomaticin (1) and gonionenin (2). The structures of these compounds were characterized on the basis of chemical and spectroscopic data. Compounds 1 and 2 have a relative stereochemistry relationship of threo/trans/threo across the mono-tetrahydrofuran ring with its two flanking hydroxyls. Compounds 1 and 2 are known, but are first isolated from this plant. In brine shrimp lethality test (BST), 1 and 2 exhibited cytotoxic activity.

• Natural Product Sciences
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• 제13권4호
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• pp.295-299
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• 2007

The methanol extract of seeds of Swietenia macrophylla King. (MESM) was studied for its antidiabetic activity in streptozotocin induced diabetic rats. It was principally aimed to correlate the efficacious role of MESM on reduction of oxidative state associated with diabetes. The extract was found to be potent antidiabetic evidenced by significant reduction of blood glucose level in diabetic rats (47.96% reduction of blood glucose level, at 300 mg/kg, on day 10). It was found that, MESM at 300 mg/kg, significantly decreased TBARS (35.03 and 22.22%) whilst increased GSH (86.75 and 31.45%), SOD (93.05 and 45.88%) and CAT (56.99 and 68.46%) levels in liver and kidney respectively in diabetic rats.

• Natural Product Sciences
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• 제24권4호
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• pp.288-292
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• 2018

High-performance countercurrent chromatography (HPCCC) coupled with reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed to isolate dihydrophaseic acid 3'-O-${\beta}$-D-glucopyranoside (DHPAG) from the extract of Nelumbo nucifera seeds. Enriched DHPAG sample (2.3 g) was separated by HPCCC using ethyl acetate/n-butanol/water system (6:4:10, v/v/v, normal-phase mode, flow rate: 4.0 mL/min) to give 23.1 mg of DHPAG with purity of 88.7%. Further preparative RP-HPLC experiment gave pure DHPAG (16.3 mg, purity > 98%). The current study demonstrates that utilization of CCC method maximizes the isolation efficiency compared with that of solid-based conventional column chromatography.

• 식물분류학회지
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• 제53권3호
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• pp.222-229
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• 2023

Ipomoea heptaphylla Sweet and Murdannia nudiflora (L.) Brenan have been discovered on arable land on Jejudo Island, Korea. Ipomoea heptaphylla can easily be distinguished from other Korean Ipomoea species by certain distinctive features, which include thin and coiled long pedicels, small corollas, and, most importantly, palmatisect leaves with five to seven lobes. Murdannia nudiflora is characterized by the absence of a rhizome, the presence of caducous bracteoles, by its bearing of more than one flower, and by its fruits with two seeds per valve and foveolate-reticulate seeds, distinguishing it from M. keisak. In this study, we report the plants I. heptaphylla and M. nudiflora and provide their morphological descriptions, photographs, distribution, and keys.

• 한국자원식물학회지
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• 제25권1호
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• pp.14-23
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• 2012

본 연구는 식 약용 등 여러 방면에서 활용성이 높은 멀구슬나무 종자의 저장방법, 온도조건 및 전처리에 따른 발아특성을 구명하고자 하였다. 종자 저장방법 중에서는 노천매장 조건에서 가장 높은 발아율을 나타내어 난저장성 종자로 추정되었다. 적정 발아온도 조건은 $35^{\circ}C$로 나타났으며, 항온조건 보다는 변온조건이 발아에 더 효과적이었다. 또한, 종피파상 및 발아촉진제를 처리한 결과, 파상처리 및 $GA_3$ 100 ppm 및 $KNO_3$ 1.0% 처리시 발아특성이 증진되었다. 이상의 결과로 보아 난저장성인 멀구슬나무 종자는 변온처리 및 파상처리와 발아촉진제 처리에 영향을 받는 것으로 나타났으나 저장방법, 온도조건 및 전처리의 복합처리에 대한 발아특성이 구명되지 않았으므로 이들의 복합처리에 따른 결과를 도출하여 묘목생산시 활용하여야 할 것이다.

• Journal of Plant Biotechnology
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• 제44권2호
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• pp.191-202
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• 2017

Chemical compounds from four different tissues of the kenaf plant (Hibiscus cannabinus), a valuable medicinal crop originating from Africa, were examined to determine its potential for use as a new drug material. Leaves, bark, flowers, and seeds were harvested to identify phytochemical compounds and measure antioxidant activities. Gas chromatography mass spectrometry analyses identified 22 different phytocompounds in hexane extracts of the different parts of the kenaf plant. The most abundant volatile compounds were E-phytol (32.4%), linolenic acid (47.3%), trisiloxane-1,1,1,5,5,5-hexamethyl-3,3-bis[(trimethylsilyl)oxy] (16.4%), and linoleic acid (46.4%) in leaves, bark, flowers, and seeds, respectively. Ultra-high performance liquid chromatography identified the major compounds in the different parts of the kenaf plant as kaemperitrin, caffeic acid, myricetin glycoside, and p-hydroxybenzoic acid in leaves, bark, flowers, and seeds, respectively. Water extracts of flowers, leaves, and seeds exhibited the greatest DPPH radical scavenging activity and SOD activity. Our analyses suggest that water is the optimal solvent, as it extracted the greatest quantity of functional compounds with the highest levels of antioxidant activity. These results provide valuable information for the development of environmentally friendly natural products for the pharmaceutical industry.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.164-173
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• 2016

수박의 형태적 변이의 유전적 원인을 분석해 보기 위해 8개 계통에서 re-sequencing을 수행하였다. 유전적 변이의 수는 염색체에 따라 다르게 나왔으며 발견된 SNP의 약 12.9%만이 유전자내에서 발견되었고 나머지는 프로모터나 유전자 사이의 지역에서 발견되었다. SNP 밀도에 대한 분석 결과 염색체 6번의 말단지역에 변이가 집중되어 있는 것을 알 수 있었다. 또한 염색체 10과 11번에 잘 보존된 지역을 발견하였다. Pathway 분석을 통해 DIMBOA(일종의 항생제)-glucoside 분해 대사가 계통간 가장 차이나는 것으로 확인되었으며 이는 각 계통의 병저항성에서 차이가날 가능성을 시사하는 것이다. 당대사 관련 유전자 변이를 분석한 결과 alpha-galactosidase 유전자에 가장 변이가 많은 것으로 밝혀졌다. 이러한 연구 결과는 육종을 분자수준에서 이해하는 데 도움을 줄 것으로 생각한다.

• Nutrition Research and Practice
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• 제7권6호
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• pp.453-459
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• 2013

The abnormal content of blood lipids often results in metabolic diseases, such as hyperlipidemia and obesity. Many agents, including natural sources from traditional food, have been developed to regulate the blood lipid contents. In this study, we examined the anti-hyperlipidemic activity of Rhynchosia nulubilis seeds pickled with brown rice vinegar (RNSpBRV), a Korean traditional pickled soybean food. Since RNSpBRV is made of R. nulubilis seeds (RNS) soaked in brown rice vinegar (BRV), we compared the anti-adipogenic activity between RNS, BRV and solid fraction of RNSpBRV (SF-RNSpBRV), liquid fraction of RNSpBRV (LF-RNSpBRV). For this, the inhibitory effect of lipid accumulation in 3T3-L1 adipocyte was checked by adding methanol extracts of mixed RNS and BRV, LF-RNSpBRV, and SF-RNSpBRV. The addition of each methanol extract up to 1 mg/ml showed no cytotoxicity on 3T3-L1 adipocyte, and approximately 20% of the lipid droplet formation was suppressed with the methanol extract of BRL or SF-RNSpBRV. The highest suppression (42.1%) was achieved with LF-RNSpBRV. In addition, mice fed a high fat diet (HFD) supplemented with 5% RNSpBRV powder led to increased high density lipoprotein (HDL) cholesterol and lower blood glucose, triglyceride, and total cholesterol compared to mice fed with a HFD diet only. Interestingly, the size of the epididymis cells gradually decreased in HFD + 1% RNSpBRV and HFD + 5% RNSpBRV-fed mice if compared those of HFD-fed mice. Taken together, these results provide evidence that RNSpBRV has a regulatory role in lipid metabolism that is related to hyperlipidemia.

• Journal of Periodontal and Implant Science
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• 제30권1호
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• pp.91-104
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• 2000

Many synthetic bone materials have been studied for their potential of regenerative effects in periodontal tissue. Safflower seeds have been traditionally used as a drug for the treatment of fracture and blood stasis in oriental medicines. The purpose of this study was to assess and compare the osseous responses in rat calvarial defects between bone substitutes such as calcium carbonate and bovine-derived hydroxyapatite and feeding of safflower seeds. The calvarial defects were made with 8 mm trephine bur in 24 Sprague-Dawley rats. Two graft materials were implanted in each experimental groups, whereas the control and safflower seed feeding groups were sutured without any other treatment. And then the rats of safflower seed feeding group were supplied with 3 g/day of safflower seeds. Each group was sacrificed at 4 weeks and 8 weeks. To study a histopathology related to bone healing and regeneration, Goldner's Masson Trichrome stain was done at each weeks. The tissue response was evaluated under light microscope. There were more osteoblastic activity, new bone formation, dense bony connective tissues in bovine-derived hydroxyapatite group compared to other groups at 8 weeks. The osseous defect area of safflower seed feeding group was filled with prominent fibrous tissues, where less inflammatory infiltration and new capillary proliferation. In the early phase of bone healing, safflower seed feeding reduces the inflammatory response and promotes the proliferation of connective tissue. These results suggest that natural bovine-derived HA and safflower seed feeding could enhance the regenerative potential in periodontal defects.