• Korean Journal of Pharmacognosy
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• v.54 no.2
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• pp.72-79
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• 2023

MRSA is Staphylococcus aureus resistant to β-lactam antibiotics, and is a worldwide infectious disease. Even with the discovery of new antibiotics, resistance develops rapidly, so new alternatives are needed. Jakyakgamcho-tang (JGT) is a combination of Jakyak and Gamcho, and has been mainly used as an antispasmodic and analgesic in oriental medicine. This study was conducted to find out whether there is an effect on MRSA in relation to the anti-inflammatory effect of JGT and the antibacterial effect of Jakyak and Gamcho found in previous studies. In this study, in order to investigate the antibacterial activity of JGT and the combined effect of existing antibiotics, after extracting JGT with 70% EtoH, the disc diffusion method, minimum inhibitory concentration (MIC), drug combination effect (FICI), and time-kill analysis (Time-kill assay), metabolic inhibition, Western blot and qRT-PCR analysis were used to confirm the antibacterial activity mechanism of MRSA of JGT. As a result of the experiment, all of MRSA showed antibacterial activity in JGT's disc diffusion method, and the MIC was 250-1000 ㎍/mL. When existing antibiotics and JGT were combined with drugs, most had synergy or partial synergy. In addition, it was confirmed that the degree of bacterial growth was suppressed over time when simultaneous administration for 24 hours. JGT showed a synergistic effect when administered together with the ATPase-inhibitor DCCD, suggesting that it affected the inhibition of ATPase. As a result of observing the expression of PBP2a, and hla protein in the JGT-treated group and the untreated control group through wstern blot, it was confirmed that the protein expression of the JGT-treated group was significantly suppressed, and the expression levels of mecA, mecR1 and hla genes were also suppressed during JGT treatment. was observed by qRT-PCR. Combining the results of the experiment, it can be seen that JGT has antibacterial activity in MRSA, and when combined with existing antibiotics, the effect was increased compared to treatment with the drug alone. This suggests that JGT can be an alternative to treatment for antibiotic resistance of MRSA.

• Natural Product Sciences
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• v.29 no.2
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• pp.98-103
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• 2023

Sphaeranthus africanus is commonly used as a traditional remedy for sore throats and pain treatment in Vietnam. The aerial parts have been studied for its anti-inflammatory and anti-proliferative properties. However, the antioxidant and antidiabetic potential of the plant has not been explored. In this work, hydrophilic extracts of the plant's aerial parts were prepared in order to investigate its antioxidant and anti-diabetic properties. Also, the cytotoxicity of the root was evaluated and compared to that of the aerial parts. All of the extracts inhibited lipid peroxidation with IC₅₀ values ranging from 2.05 to 3.56 ㎍/mL, indicating substantial antioxidant activity. At an IC₅₀ value of 4.80 ㎍/mL, the 50% ethanol extract exhibited the most potent inhibition of α-glucosidase. The cytotoxic activity of root extracts is 2 to 5-fold less than that of the aerial parts. Nevertheless, dichloromethane and ethyl acetate extracts of the root demonstrated a selective effect on leukemia cells, with no harm towards the normal HEK-293 cell line. This work provides a scientific support for the antioxidant and antidiabetic activity of the plant. Hence, it may find a promising material for the development of novel antioxidant and antidiabetic agents. More research can be conducted on the phytochemistry and anticancer activities of the plant's root.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.4A
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• pp.707-717
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• 2006

As an alternative to conventional prestressed concrete (PSC) girders, various types of PSC girders are either under development or have already been applied in bridge structures. Incrementally prestressed concrete girder is one of these newly developed girders. According to the design concept, these new types of PSC girders have the advantages of requiring less self-weight while having the capability of longer spans. However, the dynamic interaction between bridge superstructures and passing trains is one of the critical issues concerning these railway bridges designed with more flexibility. Therefore, it is very important to evaluate modal parameters of newly designed bridges before doing dynamic analyses. In the present paper, a 25 meters long full scale PSC girder was fabricated as a test specimen and modal testing was carried out to evaluate modal parameters including natural frequencies and modal damping ratios at every prestressing stage. During the modal testing, a digitally controlled vibration exciter as well as an impact hammer is applied, in order to obtain precise frequency response functions and the modal parameters are evaluated varying with construction stages. Prestressed force effects on changes of modal parameters are analyzed at every incremental prestressing stage. With the application of reliable properties from modal experiments, estimation of dynamic performances of PSC girder railway bridges can be obtained from various parametric studies on dynamic behavior under the passage of moving train. Dynamic displacements, impact factor, acceleration of the slab, end rotation of the girder, and other important dynamic performance parameters are checked with various speeds of the train.

• Journal of Korea Entertainment Industry Association
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• v.13 no.8
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• pp.267-280
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• 2019

The hybrid phenomenon of pronunciation of K-Pop lyrics, which is the diction fusion phenomenon of lyrics, is not a strategic measure for an overseas expansion but a natural formation in the course of history of K-Pop. With the debut of Seo Tai-ji and Boys in 1992, the course of K-Pop changed and settled from ballad to dance music. After three years, the success of a hip hop dance music, 'Come Back Home', opened up a door to Korean Americans who ascertained the potential competitiveness of a hip hop music in K-Pop market. And the writer insists that such advance of Korean Americans into K-Pop market is the origin of the bilingual usage and diction fusion phenomenon of K-Pop lyrics. Therefore, this research will look into the pronunciation system of Korean and English in order to understand the bilingual pronunciation system shown in the lyrics sung by Korean American hip hop singers in the 1990s. Moreover, through Korean pronunciation system, this analysis will discover the main cause of continual acceptance and application of Korean American's broken Korean in the diction of lyrics. Last but not least, this exploration will show the correlation of English pronunciation method of Korean lyrics appeared in K-Pop and the diction of Korean lyrics used by Korean American hip hop singers in late 90s. This research will identify the primary cause of the diction fusion phenomenon in the course of history of K-Pop.

• Journal of Korea Entertainment Industry Association
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• v.13 no.7
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• pp.321-346
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• 2019

The cycle of human life has a limited time to death. Everyone cannot help but think how to live in the limited time and then they try to reach toward their happiness. Their pursuit for the happiness gives rise to the new trend called 'Well-bing'. Generally speaking, it means to eat well, to feel their own satisfaction with their lives and to spend a time on doing what they want to do. However, considering their current lives which are tough and harsh, it could be impossible for them to accomplish the well-being stated above. From this point, it is natural to think what real well-being means. Its real meaning would be understood by identifying its cause if well-being is the way to happiness. Based on the thesis stated above, let me look into the desire of human being. It has both side; it should be eliminated or overcome as a deficiency and disability in human life while it could be an driving force to let the human being keep their lives. They can find the real meaning of well-being and their happiness can be realized through the control of their desires. Above all, according to the control for them, they can accomplish their happiness or fail to do it. The trend of well-being can be kept in our culture when moral value is reflected to the well-being. Finally, it orients to the purpose of happiness. It also means that everyone shares moral and positive value one another in the process of challenge to achieve their goals. In order to realize it, they should be satisfied with their current situation and have a balance with everything related to themselves.

• Analyses & Alternatives
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• v.1 no.2
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• pp.44-64
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• 2017

From the field survey of the main ethnic minority areas in the south China, it is found that political, economic, cultural and natural environmental changes are the main reasons leading to the change of customary law. The power mechanism of the customary law change include the three aspects, such as the promotion of country elite, the dominance of grassroots government, and the daily demands of the villagers, which promote the change of customary law alone or together. Through the application of customary law, the country elites can adjust and refine the rules of customary law in order to make out the new customary law and promote its development. In the current pattern of rural governance, grassroots self-government is actually the "official supervision of people's autonomy". The executive power of the grassroots government often intervene the practice of customary law and other informal rules. This is another mechanism of customary law change. Customary law arises from the practice of the daily life of the villagers. If the villagers think that the norms of customary law cannot meet the actual needs of daily life practice, the customary law will be promoted in the form of collective consultation. This is the most important dynamic practice mechanism of customary law change. Transformation and abandonment are the two ways to change customary law. No matter what kind of change does not lead to the demise of the customary law system, the demise of the customary law is only an outdated result, which is made by the universality, nature and objectivity of customary law. The procedure of customary law change is the process of continuation and rebirth about customary law. The result of the change is to produce the new customary law of keeping pace with the times, and the customary law will be presented with new content and form after the change. The continuation of customary law means the inheritance of traditional customary law, but it is based on the transformation of traditional customary law. The rebirth of customary law means that the traditional customary law is completely discarded. But it will produce new customary law rules and be based on the needs of social life practice. Customary law occupies a pivotal position in the normative system and the national law cannot be replaced. The purpose of customary law change will let the customary rules better adapt to the development of modern society, adjust the social relations more reasonably and better meet people's needs of production and life, which is decided by the character of customary law.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.318-318
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• 2022

Basil(Ocimum basilicum L.) is an annual herb that grows wild in hot and humid regions such as tropical Asia and Africa. This study was carried out for resource discovery, propagation, and DB construction of aromatic plants. In order to evaluate the genetic characteristics, 28 kinds of basil seeds were supplied from the Center for Genetic Resources. For basil characteristics, planting date, plant height, growth shape, leaf length, leaf shape, petiole color, petiole, stem color, flower color, after harvest, Ml length including roots, flower length, stem thickness and biological weight were measured. For anthocyanin analysis, only basil with purple color was selected, anthocyanins were extracted with 60% ethanol containing 1% citric acid, a standard quantitative curve was prepared with cyanidin-3-glucoside, and absorbance was measured at 525 nm. Basil planting started around June 16, and the flowering period lasted for a total of 18 days from July 19 to August 5. On August 6, when flowering was completed, morphological characteristics including flower color were measured at the site by an object. The measured basil plant length was 26.6-59.6 cm, leaf length 2.0-7.9 cm, leaf width 0.7-5.2 cm, and petiole 1.3-3.9 cm. The growth pattern of basil was generally straight, medium in shape. The color of the leaves was observed evenly by mixed green, purple, purple and green, and the recesses in the shape of the leaf were also conspicuously observed. A total of three basil flower colors were observed: white, light pink(149P 10P/84), and purple(37V 2.5RP 4/12). During the second growth survey after harvest, the total length including the basil roots is 26.7-52.0 cm, the flower length is 8.2-29.3cm, the stem thickness is 7.1-15.9mm, the number of stem nodes is 3-12 nodes, and the total weight of the basil was measured to be about 218-1540 g. There are a total of 16 types of basil expressed in purple, and the anthocyanin content was measured to be 73.74 ~ 380.72 mg/100g.

• Journal of The Korean Association For Science Education
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• v.43 no.6
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• pp.573-582
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• 2023

This study aimed to explore the potential use of artificial intelligence in science education for gifted students by analyzing the structure of abstracts written by students at a gifted science academy and comparing the performance of various elements extracted using AI. The study involved an analysis of 263 graduation theses from S Science High School over five years (2017-2021), focusing on the frequency and types of background, objectives, methods, results, and discussions included in their abstracts. This was followed by an evaluation of their accuracy using AI classification methods with fine-tuning and prompts. The results revealed that the frequency of elements in the abstracts written by gifted students followed the order of objectives, methods, results, background, and discussions. However, only 57.4% of the abstracts contained all the essential elements, such as objectives, methods, and results. Among these elements, fine-tuned AI classification showed the highest accuracy, with background, objectives, and results demonstrating relatively high performance, while methods and discussions were often inaccurately classified. These findings suggest the need for a more effective use of AI, through providing a better distribution of elements or appropriate datasets for training. Educational implications of these findings were also discussed.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.4
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• pp.299-306
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• 2023

In this study, exosomes were isolated by ultrafiltration from Lactobacillus rhamnosus J2K-821 and their various effects for skin were evaluated. Their size and concentration were identified 50 ~ 200 nm and 3.22 × 10⁸ particles/mL, respectively through nanoparticle tracking analysis. In order to verify the inflammatory relief effect of Lactobacillus rhamnosus-derived exosomes (LRDEs), their nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS) was confirmed. It was revealed that they inhibited NO production in a concentration-dependent manner. To evaluate the antioxidant activity and skin barrier improvement effect of LRDEs, their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and hyaluronidase inhibitory activity were confirmed. It was also revealed that their activities were increased by concentration-dependent manner. Through these results, It is believed that LRDEs can be used as a effective natural cosmetic ingredient for anti-inflammation, antioxidation and skin barrier improvement.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.11-11
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• 2020

Background : The genus Cypripediums are typically adapted to the low temperature and cold climates of the high land in Korea. The species which were reported in Korean peninsula are C.japonicum, C.macranthos, C.calceolus and C.guttatum. We had already reported the successful germination of the C.macranthos and C.guttatum. The small spotted lady's slipper orchid(C.guttatum) is most endangered plants to extinction of which population was reported less than 100 in Korea. The objective of the present research is restoration of the C.guttatum in ex situ and adaptation in high land and nursery. Methods and Results : The 350 seedlings of C.guttatum were transplanted in soil at alpine area(altitude 750m) and the nursery of Korea National Arboretum(KNA) in YangPyeong city on May 2019 and 2020 in order to verify the possibility of ex situ restoration. The sprouted ratio of the seedlings were less than 10% in the alpine area and 83% in the nursery of KNA. The reasons of the diverse survival ratio are presumed as the low moisture content in the soil and heavy shade of the alpine area. The 15 cypripedium species and 29 hybrids were also effectively adapted in the KNA rare plant garden. Conclusion : These results demonstrated the difficulties of the restoration for small spotted lady's slipper orchid in the natural habitation. From the above results, we may conclude that the critical factors of the genus Cypripedium are intensive care for the fragile seedlings during the 1^st year acclimatization.