Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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A Study on Anti-Inflammatory, Antioxidant Activity and Skin Barrier Improvement of Lactobacillus rhamnosus-derived Exosomes

락토바실러스 람노서스 유래 엑소좀의 항염증, 항산화 효능 및 피부 장벽 개선에 관한 연구

  • 김희식 ((주)제이투케이바이오 기술연구소) ;
  • 현종우 ((주)제이투케이바이오 기술연구소) ;
  • 김민우 ((주)제이투케이바이오 기술연구소) ;
  • 이지연 ((주)제이투케이바이오 기술연구소) ;
  • 장준환 ((주)제이투케이바이오 기술연구소) ;
  • 배준태 ((주)제이투케이바이오 기술연구소)
  • Received : 2023.08.25
  • Accepted : 2023.10.06
  • Published : 2023.12.30
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Abstract

In this study, exosomes were isolated by ultrafiltration from Lactobacillus rhamnosus J2K-821 and their various effects for skin were evaluated. Their size and concentration were identified 50 ~ 200 nm and 3.22 × 108 particles/mL, respectively through nanoparticle tracking analysis. In order to verify the inflammatory relief effect of Lactobacillus rhamnosus-derived exosomes (LRDEs), their nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS) was confirmed. It was revealed that they inhibited NO production in a concentration-dependent manner. To evaluate the antioxidant activity and skin barrier improvement effect of LRDEs, their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and hyaluronidase inhibitory activity were confirmed. It was also revealed that their activities were increased by concentration-dependent manner. Through these results, It is believed that LRDEs can be used as a effective natural cosmetic ingredient for anti-inflammation, antioxidation and skin barrier improvement.

본 연구에서는 락토바실러스 람노서스(Lactobacillus rhamnosus) J2K-821 균주로부터 한외여과를 통해 엑소좀을 분리하고, 피부에 대한 여러 효능을 평가했다. 나노입자추적분석 결과 3.22 × 108 particles/mL 농도의 크기가 50 ~ 200 nm인 락토바실러스 람노서스 유래 엑소좀(LRDEs)을 확인하였다. 염증 완화 효과를 검증하기 위해 LRDEs를 lipopolysaccharide (LPS)로 염증반응을 유도시킨 RAW 264.7 대식세포에서의 nitric oxide (NO) 생성 저해능을 확인한 결과, 농도 의존적으로 NO 생성을 저해하였다. 그리고 항산화 및 피부 장벽 개선 효능을 평가하기 위해 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼 소거 활성, hyaluronidase 억제 활성을 확인한 결과, 농도 의존적으로 항산화 및 hyaluronidase 억제 활성이 증가하는 것을 확인하였다. 이를 통하여 LRDEs는 항염증, 항산화 및 피부 장벽 개선에 효과적인 천연 화장품 소재로 사용 가능할 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 중소벤처기업부와 중소기업기술정보진흥원의 "지역특화산업육성+(R&D, S3264754)" 사업의 지원을 받아 수행된 연구결과입니다.

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