• 한국실내디자인학회논문집
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• 제26권4호
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• pp.30-41
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• 2017

Sunlight is perceived by human beings first through the epidermis to space, and is a non - material medium that provides physical awareness of space, diversified expression of spaces, and plenty experience. The purpose of this study is to investigate the characteristics of active building skin based on the inflow of natural light required by humans, looked through among the works of Jacques Herzog & Pierre de Meuron, which show the characteristics of active building skin, TEA(Tenerife Espacio de las Artes, 2008), Messe Basel New Hall (2013) and Elbphilharmonie (2016). First, the interaction between Sunlight and space is divided into spatial characteristics and sensitivities according to their concepts, properties, and characteristics. The characteristics of active skin by light are classified into a physical approach and a constructive approach. Second, (El Croquis 152/153) and analyzed the images, detail drawings, and elevations, and simulated them in 3D to express the relationship between light and active building skin. Third, the changes of light intensity, light color, and distribution of light according to the time of light entering and the skin are determined from 6:00 am to 6:00 pm. Fourth, the images taken from January 30th to February 7th, 2017 on the site were compared with the computer simulated images, and the relationship between active skin and light was compared. This study is to recognize the existence and necessity of light required for human being through the activated epidermis differentiated from the limited or closed epidermis focused on information transmission, I would like to emphasize that I would like to take a step closer to the necessity and possibility of new attempts and developments so that I can feel the various experiential spaces by.

• Journal of Advanced Marine Engineering and Technology
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• 제38권7호
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• pp.883-889
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• 2014

고속유체 환경에서 압력차에 의한 국부적 비등으로 캐비테이션 침식 손상이 발생한다. 캐비테이션은 유체의 압력, 속도, 온도, pH, 그리고 매질 등의 다양한 환경에 영향을 받는다. 특히 해수 용액에서 캐비테이션 환경에 노출될 경우 염소이온에 의한 부식이 캐비티에 의한 침식 손상을 가속화 시킨다. 따라서 본 연구는 마르텐사이트계 스테인리스강에 대해 천연 해수 용액에서 적용 전류밀도와 캐비테이션 시간에 따른 시편 손상 경향을 규명하였다. 정전류 실험 결과 캐비테이션 조건에서 정적인 조건에 비해 비교적 적은 손상 경향이 나타났다. 또한 캐비테이션 실험 결과 3시간부터 급격하게 무게 감소량, 캐비테이션 손상률, 손상깊이가 증가하였다.

• Journal of Plant Biotechnology
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• 제45권2호
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• pp.146-153
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• 2018

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

• Natural Product Sciences
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• 제10권6호
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• pp.289-295
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• 2004

Myristica fragrans seed from Myristica fragrans Houtt (Myristicaceae) has various pharmacological activities peripherally and centrally. The present study aims to investigate the effect of the methanol extract of Myristica fragrans seed (MF) on N-methyl-D-aspartate (NMDA)-induced neurotoxicity in primary cultured rat cerebellar granule neuron. MF, over a concentration range of 0.05 to $5\;{\mu}g/ml$, inhibited NMDA (1 mM)- induced neuronal cell death, which was measured by trypan blue exclusion test and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. MF $(0.5\;{\mu}g/ml)$ inhibited glutamate release into medium induced by NMDA (1 mM), which was measured by HPLC. Pretreatrnent of MF $(0.5\;{\mu}g/ml)$ inhibited NMDA (1 mM)-induced elevation of cytosolic calcium concentration $([Ca^{2+}]_c)$, which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that MF prevents NMDA-induced neuronal cell damage in vitro.

• 생약학회지
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• 제38권4호
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• pp.312-320
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• 2007

The screening of anti-cancer activity for the MeOH extracts of 163 natural sources, which were registered as edible plants by Korea Food & Drug Administration, exhibited 9 extracts to have significant inhibitory effects on farnesyl-protein transferase (FPTase) and phosphatase of regenerating liver-3 (PRL-3). In order to confirm the inhibitory activity of these active extracts, the activity assay was repeated for some fractions obtained from the active extracts using Medium Pressure Liquid Chromatography (MPLC). Some fractions of Carya illinoensis, Chlorella vulgaris, Panicum miliaceum, Perilla frutescens, Rosmarinus officinalis showed over 50% inhibitory activity on FPTase as well as those of Capsella bursa-pastoris, C. illinoensis, C. vulgaris, Coix lacrymajobi, Myristica fragrans, P. miliaceum, R. officinalis did over 50% inhibitory activity on PRL-3.

• 대한지하수환경학회지
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• 제5권1호
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• pp.30-36
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• 1998

저분자량 유기산을 이용한 in-situ flushing 토양세척기법의 실제 현장 적용성 검토를 위해 실험실에서 회분식 실험을 통하여 적당한 세척제를 선정하고 이에 따른 여러 가지 운전변수를 찾아보았다. 대상 중금속으로는 Cu를 택했고, 세척제로는 실제 토양환경에 영향이 비교적 적은 저분자량 유기산인 acetic, citric, oxalic, succinic acid를 이용했다. 유기산을 이용하여 처리한 결과 실험에 사용된 유기산들 모두 1 mM의 저농도에서는 효과를 나타내지 못했고 50 mM 이상의 고농도에서 보다 좋은 제거율을 보였는데, 50 mM과 100mM의 경우 제거율이 큰 차이를 보이지 못했다. citric arid와 oxalic acid의 경우 중성 및 약산성 상태에서 최대의 제거율이 나타났고 특히 citric arid의 경우 87.1%의 높은 제거율을 보였다. 유기산이 없을때는 강산성 상태에서 Cu의 최고제거율이 70% 정도였다. 그러나 각 경우에서의 speciation을 살펴보면 유기산이 없을 경우에는 추출된 Cu의 대부분이 유해한 free ion상태로 존재하게 되고 유기산이 있을 경우에는 추출된 Cu의 대부분이 유기산과 complex를 이룬다는 것을 알 수 있었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.119-123
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• 2005

Benomyl-resistant mutants of entomopathogenic fungus, Metarhizium anisopliae were isolated and their physiological characteristics were investigated. These militants were obtained spontaneously or by UV irradiation in benomyl-treated media. Four spontaneous (S-2, S-11, S-18, S-19) and four UV-induced (UV-4, UV-5, UV-19, UV-24) mutants, which grow stably and normally were selected. No significant differences in conidia or hyphal shape, conidia viability, mycelial biomass, or virulent to the diamondback moth were observed between the wild type and their mutants. But differently from the mycelial growth of other benomyl-resistant mutants which was slower than that of the wild type on a modified Czapek-Dox, SDAY, $4\%$ chitin, or $1\%$ skim milk medium, that in the spontaneous mutants, S-18 and S-19, did not show any difference from the wild type. Especially, S-18 and S-19 grew well at benomyl concentrations up to 50 times or higher than that which inhibits wild type proliferation. These results suggested that S-18 and S-19 could potentially be used with the fungicide, benomyl.

• Journal of Microbiology and Biotechnology
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• 제26권9호
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• pp.1570-1578
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• 2016

Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. gallica TCK was treated with N-methyl-N-nitro-N-nitrosoguanidine and ultraviolet light. Among the mutants isolated, T906 was found to be a high-production strain of laccases. The mutant strain T906 was stabilized via dozens of passages, and the selected ones were further processed for optimization of metallic ion, inducers, and nutritional requirements, which resulted in the optimized liquid fermentation medium MF9. The incubation temperature and pH were optimized to be 30℃ and 4.5, respectively. The mutant strain T906 showed 3-times higher laccase activity than the original strain TCK under optimized conditions, and the maximum laccase production (303 U/ml) was accomplished after 13 days. The extracellular laccase isoenzyme 1 was purified and characterized from the two strains, respectively, and their cDNA sequence was determined. Of note, the laccase isoenzyme 1 transcription levels were overtly increased in T906 mycelia compared with values obtained for strain TCK. These findings provide a basis for C. gallica modification for the production of high laccase amounts.

• 보존과학회지
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• 제8권1호
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• pp.1-9
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• 1999

석굴암은 해체, 복원 및 수차례에 걸친 보수과정을 거쳐 지금에 이르렀다. 현재와 같은 비교적 안정된 환경이 이루어지기 전 까지 오랜 시간에 걸친 자연적인 풍화와 사람에 의한 영향 등에 의해 많은 변화를 겪어 왔다. 석굴암을 이루는 암석은 중립 내지 조립질의 화강섬록암으로 대체로 갈색에서 암갈색에 이르는 풍화면을 보이는데 이는 이 암석의 주구성광물인 장석류의 화학적 풍화에 기인한다. 또한 콘크리트돔이나 균열된 곳에 사용된 시멘트의 풍화물에 의한 표백 및 백화 현상 등에 의해 부분적으로 우백색을 띠거나 때로는 매우 검게 변색되어 있기도 한다. 여러 곳에 크고 작은 균열들이 발달되어 있으며 이는 주로 오랜 풍화작용에 의한 영향과 해체-복원 및 보수 과정중의 충격등에 기인하는 것으로 생각된다. 이러한 일반적인 현상들은 대부분이 현재와 같은 비교적 안정된 환경이 갖추어지기 전에 이루어진 것으로 생각된다. 그러나 앞으로의 더 나은 보전을 위해서는 내부 공기의 온도와 습도, 특히 위치에 따른 상대습도의 지속적인 평형유지, 구조적 균형유지, 그리고 진동 등에 의한 영향을 체계적이고 계속적으로 측정하여 대책을 수립하여야 할 것으로 생각된다.

• 방사성폐기물학회지
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• 제3권2호
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• pp.135-148
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• 2005

방사성 폐기물의 지층 처분장 건설 및 운영을 위한 개념 선정 단계에서는 폐기물 운반 및 거치뿐 아니라, 처분장의 건설/운영/폐쇄 기간 동안 지하 처분장의 작업 환경 및 위생, 안전, 그리고 처분장내의 수분 제거와 같은 향후 처분장의 환경을 위해 처분장 환기시스템에 대한 고려가 향후 처분장의 환경을 위해 반드시 필요하다. 본 논문은 동굴처분 방식의 중-저준위 처분장 및 지하 심부에 위치하게 될 고준위 처분장에 대한 환기시스템 개념설계 기준 및 요구사항에 대한 내용이다. 방사성폐기물 처분장의 환기 시스템에서 가장 주된 기본 설계 개념은 처분장 건설과 폐기물저장을 위한 작업활동을 위해 각각 독립적이고 분리된 환기시스템을 적용하여야 한다는 것이다. 본 논문에서는 방사성폐기물 처분장의 환기시스템의 설계과정에 대해 기술하고 환기회로 모델링 방법, 자연 환기, 환기 모니터링 시스템과 실시간 환기 시뮬레이션, 화재 시뮬레이션 및 비상 방재 시스템에 관한 사항도 논의하였다