• Title/Summary/Keyword: natural medium

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Establishment of propagation system for in vitro calla plants(Zantedeschia spp.) by using auxin and cytokinin hormones treatments (오옥신과 사이토키닌 호르몬 처리를 이용한 칼라 기내 식물체 대량증식체계 확립)

  • Lee, Sang Hee;Kim, Young Jin;Yang, Hwan Rae;Kim, Jong Bo
    • The Journal of the Convergence on Culture Technology
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    • v.4 no.4
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    • pp.325-329
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    • 2018
  • There are two kinds of calla belonging to the Zantedeschia spp. depending on the growing condition. Between them, various colored calla with flower shape and flower color are very popular as cut flowers. However, colored calla is very low in natural reproduction rate, and it takes a long time to obtain a flowering bulb. So it is urgent to establish a large breeding system of good varieties. In this study, various plant growth hormone treatments were carried out to improve the growth of the plants in calla. Root formation was the highest at 81.5 % in basal MS medium, and the formation of shoot and multiple shoots were the highest at 100.0 % and 36.4 % in the combined treatment of BA 2.0 mg/L and IBA 1.0 mg/L, respectively. In fresh weight, the highest growth rate was observed with 11.2 times increase in BA 2.0 mg/L single treatment. Auxin and cytokinin mixed treatment were widely used in previous studies and positive effects on the growth of calla plants will help to establish the micro-propagation system.

An Ethanol Extract of the Brown Seaweed Hizikia fusiformis and Its Active Constituent, Fucosterol, Extend the Lifespan of the Nematode Caenorhabditis elegans (갈조류 톳(Hizikia fusiformis)의 에탄올추출물 및 이의 활성성분 fucosterol에 의한 예쁜꼬마 선충의 수명 연장)

  • Oktaviani, Diyah Fatimah;Bae, Young-Seuk;Meinita, Maria Dyah Nur;Moon, Il Soo;Hong, Yong-Ki
    • Journal of Life Science
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    • v.29 no.10
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    • pp.1120-1125
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    • 2019
  • The short-lived nematode Caenorhabditis elegans has been used as a model organism for many studies, including lifespan extension. To screen common seaweeds for natural anti-aging agents, the lifespan of C. elegans (N2 wild-type strain) was measured by its hatch rate, growth rate, survival rate, chemotaxis, brood size, and egg-laying time after exposure to nematode growth medium (NGM) containing seaweed extracts. Approximately 30 animals synchronized at the first larval stage were incubated until they reached their adult stages before laying their eggs and were transferred to fresh NGM every 3 days. We also identified the major active compound from the seaweed by gas chromatography-mass spectrometry and tested its optimal dose for longevity. Of 13 common seaweed species, an ethanol extract of the brown seaweed Hizikia fusiformis showed the greatest effect on hatching, growth, and survival rates. The lifespan of C. elegans was significantly expanded 1.54-fold and 1.23-fold in the presence of the ethanol extract (0.05 mg/ml) and the main active component, fucosterol (0.05 mg/ml), respectively. Exposure to the ethanol extract also increased chemotaxis 1.13-fold, decreased brood size 0.74-fold, and shortened egg-laying time 0.96-fold. These results suggest that the aquaculturable H. fusiformis may be a promising source of a diet supplement to support health care.

Dynamic Analysis on Electricity Demands for the Steel Industry in Korea: Comparison between SMEs and Large Firms (우리나라 철강산업의 전력수요에 대한 동태 분석: 중소기업과 대기업 간 비교)

  • Li, Dmitriy;Bae, Jeong Hwan
    • Environmental and Resource Economics Review
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    • v.29 no.4
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    • pp.499-520
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    • 2020
  • Input ratio of electricity to other production inputs in the Korean manufacturing sector has been higher than for the other OECD countries. In addition, electricity prices in Korea has been relatively lower than the average of OECD countries. Moreover, electricity sector is responsible for most CO2 emissions in Korea as coal and natural gas account 41.9% and 26.8% of electricity production as of 2018. Therefore, it looks inevitable to raise the electricity tariff for the manufacturing sector in Korea, but there is a concern that increase in the electricity tariff might affect small and medium enterprises (SMEs) more than large firms. This study estimates electricity demand's price and output elasticities for large firms and SMEs in steel industry by employing a time varying parameter model (Kalman filter). The analysis shows that changes in output levels regardless of firms' size affect electricity demands more significantly than do changes in electricity prices. Second, large firms have higher variances for both price and output elasticities of electricity demand. Third, large firms have higher price elasticity but lower output elasticity of electricity demand relative to SMEs. Policy implications are suggested in association with how to reduce electricity demands in the energy-intensive industry.

Consistent Boundary Condition for Horizontally-Polarized Shear (SH) Waves Propagated in Layered Waveguides (층상 waveguide에서의 SH파 전파 해석을 위한 경계조건)

  • Lee, Jin Ho
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.34 no.2
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    • pp.113-120
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    • 2021
  • The wave-propagation phenomenon in an infinite medium has been used to describe the physics in many fields of engineering and natural science. Analytical or numerical methods have been developed to obtain solutions to problems related to the wave-propagation phenomenon. Energy radiation into infinite regions must be accurately considered for accurate solutions to these problems; hence, various numerical and mechanical models as well as boundary conditions have been developed. This paper proposes a new boundary condition that can be applied to scalar-wave or horizontally-polarized shear-wave (or SH-wave) propagation problems in layered waveguides. A governing equation is obtained for the SH waves by applying finite-element discretization in the vertical direction of the waveguide and subsequently modified to derive the boundary condition for the infinite region of the waveguide. Using the orthogonality of the eigenmodes for the SH waves in a layered waveguide, the new boundary condition is shown to be equivalent to the existing root-finding absorbing boundary condition; further, the accuracy is shown to increase with the degree of the new boundary condition, and its stability can be proven. The accuracy and stability are then demonstrated by applying the proposed boundary condition to wave-propagation problems in layered waveguides.

An Analysis of Influences of Education Satisfaction of Beauty Salon Franchises on Turnover factors Mediated by Education need (프랜차이즈 직영점 미용실의 교육만족도가 교육요구도를 매개로 이직요인에 미치는 영향)

  • Kim, Min-seok;Ko, Kyoung-sook
    • Journal of Convergence for Information Technology
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    • v.11 no.5
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    • pp.232-241
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    • 2021
  • This study was conducted to prepare a countermeasure for turnover due to the effect of education satisfaction at franchise stores on turnover factors through the medium of education requirements. In the method of collecting data, 237 copies of the questionnaire method were used as the final data. The results are first, the analysis of educational requirements in general characteristics shows that academic background (F=5.655, p<).01), Analysis of the factors for turnover shows that position (t=-2.809, p<).Significant differences were shown in 01. Second, educational satisfaction is the educational requirement (r=-.16, p<).The correlation between 05) and turnover factors (r=-.18) is significant, and the educational requirement is turnover factors (r=.53, p<).It is shown to be a significant static correlation with 01. Third, designers (B=.25), t=2.332, p<.05) and educational requirements (B=.88, t=9.313, p<).001) has been shown to have significant static effects. Fourth, the educational requirements for educational satisfaction and turnover factors are significant (β=.52, p<).We can see that it is fully mediated by 001.Therefore, it is believed that it will be possible to investigate quarterly satisfaction and demand after communication and training with workers at direct stores, and reduce turnover through various curriculum education.

Anisotropic Compression Behavior and Phase Transition of Sepiolite Under Moderate Pressure Conditions (천부지권 압력 하 해포석의 비등방적 압축 특성 및 상전이 연구)

  • Seohee, Yun;Yongjae, Lee
    • Korean Journal of Mineralogy and Petrology
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    • v.35 no.4
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    • pp.423-430
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    • 2022
  • Pressure-dependent elastic behavior and chemical reaction of natural sepiolite (Mg8Si12O30(OH)4·12H2O) was studied under two different pressure-transmitting medium (PTM) conditions using synchrotron X-ray powder diffraction. Under non pore-penetrating silicone oil PTM, we observed that the b-axis length increases up to ca. 3.6 GPa, marking an anisotropic compression region with negative linear compressibility of βb= -0.0012 GPa-1, which then decreases at 7.7 GPa. Under pore-penetrating water PTM, the anisotropic compression behavior is enhanced with doubled negative linear compressibility of βb= -0.0025 GPa-1 up to 3.2 GPa, where transformation into stevensite is observed upon ex-situ temperature treatment at 280 ℃ as confirmed via XRD and SEM. Derived bulk moduli (K0) and linear compressibilities (β) were compared to other structurally and chemically related minerals.

Production of Carotenoids by Bacteria; Carotenoid Productivity and Availability (박테리아에 의한 카로티노이드 생산; 카로티노이드 생산성 및 활용 가능성)

  • Choi, Seong Seok;Kim, Gun-Do
    • Journal of Life Science
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    • v.32 no.5
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    • pp.411-419
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    • 2022
  • Carotenoids are red, orange, and yellow fat-soluble pigments that exist in nature, and are known as physiologically active substances with various functions, such as provitamin A, antioxidant, anti-inflammatory, and anticancer. Because of their physiological activity and color availability, carotenoids are widely used in the food, cosmetics, and aquaculture industries. Currently, most carotenoids used industrially use chemical synthesis because of their low production cost, but natural carotenoids are in the spotlight because of their safety and physiologically active effects. However, the production of carotenoids in plants and animals is limited for economic reasons. Carotenoids produced by bacteria have a good advantage in replacing carotenoids produced by chemical synthesis. Since carotenoids produced from bacteria have limited industrial applications due to low productivity, studies are continuously being conducted to increase the production of carotenoids by bacteria. Studies conducted to increase carotenoid production from bacteria include the activity of enzymes in the bacterial carotenoid biosynthesis pathway, the development of mutant strains using physical and chemical mutagens, increasing carotenoid productivity in strain construction through genetic engineering, carotenoid accumulation through stress induction, fermentation medium composition, culture conditions, co-culture with other strains, etc. The aim of this article was to review studies conducted to increase the productivity of carotenoids from bacteria.

Elastic Wave Characteristics According to Cementation of Dissolved Salt (용해된 소금의 고결화에 따른 탄성파 특성)

  • Eom, Yong-Hun;Truong, Q. Hung;Byun, Yong-Hoon;Lee, Jong-Sub
    • Journal of the Korean Geotechnical Society
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    • v.25 no.5
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    • pp.75-86
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    • 2009
  • Salt, one of the most common soluble materials in engineering soil, may have an effect on mechanical behaviors of soils under its cementation process. In order to investigate this natural phenomenon, non-soluble material by using glass beads is mixed with salt electrolyte and cemented by using oven to evaporate water. Three different sizes of glass bead particles, 0.26, 0.5, and 1.29 mm, with different salt concentration, 0, 0.1, 0.2, 0.5, 1.0, and 2.0M, are explored by using P- and S-waves, excited by bender elements and piezo disk elemets, respectively. The velocities of the P-wave and S-wave of the particulate medium cemented by salt show three stages with the degree of saturation: 1) S-wave velocities increase while P-wave velocities reduce with degree of saturation changing from 100% to 90%; 2) Both velocities are stable with degree of saturation varying from 90% to 10%; 3) The velocities change enormously when the specimens are nearly dry with degree of saturation from 10% to 0%. Besides, the resonance frequencies of S-wave show similar stages to the S-wave velocities. This study demonstrates meaningful trends of elastic wave characteristics of geo-materials according to the cementation of dissolved salt.

Antioxidant effect of ergothioneine on in vitro maturation of porcine oocytes

  • Ji-Young Jeong;Lian Cai;Mirae Kim;Hyerin Choi;Dongjin, Oh;Ali Jawad;Sohee Kim;Haomiao Zheng;Eunsong Lee;Joohyeong Lee;Sang-Hwan, Hyun
    • Journal of Veterinary Science
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    • v.24 no.2
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    • pp.24.1-24.13
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    • 2023
  • Background: Ergothioneine (EGT) is a natural amino acid derivative in various animal organs and is a bioactive compound recognized as a food and medicine. Objectives: This study examined the effects of EGT supplementation during the in vitro maturation (IVM) period on porcine oocyte maturation and subsequent embryonic development competence after in vitro fertilization (IVF). Methods: Each EGT concentration (0, 10, 50, and 100 μM) was supplemented in the maturation medium during IVM. After IVM, nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels of oocytes were investigated. In addition, the genes related to cumulus function and antioxidant pathways in oocytes or cumulus cells were investigated. Finally, this study examined whether EGT could affect embryonic development after IVF. Results: After IVM, the EGT supplementation group showed significantly higher intracellular GSH levels and significantly lower intracellular ROS levels than the control group. Moreover, the expression levels of hyaluronan synthase 2 and Connexin 43 were significantly higher in the 10 μM EGT group than in the control group. The expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and NAD(P)H quinone dehydrogenase 1 (NQO1) were significantly higher in the oocytes of the 10 μM EGT group than in the control group. In the assessment of subsequent embryonic development after IVF, the 10 μM EGT treatment group improved the cleavage and blastocyst rate significantly than the control group. Conclusions: Supplementation of EGT improved oocyte maturation and embryonic development by reducing oxidative stress in IVM oocytes.

Data-Driven Technology Portfolio Analysis for Commercialization of Public R&D Outcomes: Case Study of Big Data and Artificial Intelligence Fields (공공연구성과 실용화를 위한 데이터 기반의 기술 포트폴리오 분석: 빅데이터 및 인공지능 분야를 중심으로)

  • Eunji Jeon;Chae Won Lee;Jea-Tek Ryu
    • The Journal of Bigdata
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    • v.6 no.2
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    • pp.71-84
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    • 2021
  • Since small and medium-sized enterprises fell short of the securement of technological competitiveness in the field of big data and artificial intelligence (AI) field-core technologies of the Fourth Industrial Revolution, it is important to strengthen the competitiveness of the overall industry through technology commercialization. In this study, we aimed to propose a priority related to technology transfer and commercialization for practical use of public research results. We utilized public research performance information, improving missing values of 6T classification by deep learning model with an ensemble method. Then, we conducted topic modeling to derive the converging fields of big data and AI. We classified the technology fields into four different segments in the technology portfolio based on technology activity and technology efficiency, estimating the potential of technology commercialization for those fields. We proposed a priority of technology commercialization for 10 detailed technology fields that require long-term investment. Through systematic analysis, active utilization of technology, and efficient technology transfer and commercialization can be promoted.