• Journal of Digital Convergence
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• v.17 no.7
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• pp.395-400
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• 2019

Contemporary society, the era of the 4th industrial revolution, has been enjoying a boom in the cosmetics industry due to the launch of cosmetics through new technology development due to the interest in the beauty industry. However, the cosmetics industry is booming, but dry eye syndrome, skin trouble, Hormonal disturbances, and many other side effects. In order to prove the universal use value of pine needles as a natural cosmetic material that can complement the side effects of existing chemical ingredients cosmetics harmful to human body, and to propose a roll model of desirable natural cosmetics, DPPH radical scavenging activity, ABTS assay, and flavonoid content were studied. The results showed that both the DPPH radical and ABTS radical scavenging activity increased in both the $70^{\circ}C$ and $100^{\circ}C$ pine water hot water extracts. The extracts from the pine tree extracts at $70^{\circ}C$ and $100^{\circ}C$ showed $32.0{\pm}2.5mg\;QE/ml$ and 116.8 A total flavonoid of ${\pm}5.5mg\;QE/ml$ was detected. Therefore, it is considered that the pine needle hydrothermal extract having a large amount of antioxidant which is advantageous to human body is highly applicable as a natural cosmetic material harmless to the human body.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.1
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• pp.17-27
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• 2023

A study was performed to increase skin permeation of tocopheryl acetate, an oil-soluble component among tocopheryl acetate, salicylic acid, and niacinamide, which are the active ingredients of the anti-hair loss toner. As a method of transparently solubilizing tocopheryl acetate while containing salicyl acid and niacinamide, we used a swollen micelle structure that increased the size of the micelle formed by the aggregation of surfactants. The prepared swollen micelle solution contains three kinds of active ingredients, and poloxamer 407 and octyldodeceth-16 were used as surfactants to increase transparent properties and stability. In addition, isostearic acid was used as a co-surfactant to increase the size of micelles. To evaluate the physical properties of the prepared swollen micelles, turbidity at room temperature (25 ℃) was measured. The Franz diffusion cell method was used to evaluate the skin permeation rate of tocopheryl acetate among the hair-loss prevention components contained in swollen micelles. After 24 h, tocopheryl acetate showed a 6-fold improvement in skin permeation compared to the control group. Therefore, it can be seen that the swollen micelles developed in this study can be applied to hair-loss prevention products or solubilized formulations of various functional cosmetics.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.3
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• pp.255-263
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• 2017

In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities ($FSC_{50}$) of non-fermented and fermented extracts were 42.3 and $34.5{\mu}g/mL$, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) in $Fe^{3+}-EDTA/H_2O_2$ system of non-fermented and fermented extracts were 2.6 and $2.5{\mu}g/mL$, respectively. The activity after fermentation was approximately 4.2% higher. In the $^1O_2$-induced cellular damage of erythrocytes, the cellular protective effects (${\tau}_{50}$) of non-fermented and fermented extracts were 126.4 and 173.0 min at $50{\mu}g/mL$, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than $(+)-{\alpha}$-tocopherol (${\tau}_{50}=43.4min$), known as a lipophilic antioxidant at $50{\mu}g/mL$. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities ($IC_{50}$) of non-fermented and fermented extracts were 873.6 and $687.8{\mu}g/mL$, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.3
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• pp.307-317
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• 2019

To develop sustainable new natural anti-aging ingredients from Korean native plants, we investigated the cultivation potential of Nymphoides indica using the eco-friendly aquaponics system, and tested the anti-aging effects from N. indica extracts. N. indica could be grown in aquaponics system using floating leaved deep water culture method, and propagated through rhizome propagation. It was confirmed that the nitrate ($80{\mu}g/mL$), potassium ($63.5{\mu}g/mL$) and water temperature ($25^{\circ}C$) greatly affected the cultivation of the N. indica. In addition, synergistic effects were found when two major components (3,7-di-O-methylquercetin-4'-O-${\beta}$-glucoside & sweroside) were present at more than about $5{\mu}g/mL$. The extract had a significant effect on the recovery of skin cells damaged by environmental pollutant such as $benzo[{\alpha}]pyrene$, ammonium nitrate, formaldehyde. It also suppressed $PGE_2$, $TNF-{\alpha}$ and COX-2, and inhibited the production of MMP-1. Taken together, the results suggested that the standardized extracts of N. indica cultivated in the aquaponics has considerable potential as a new cosmetics ingredient with an anti-aging effect.

• Journal of Convergence for Information Technology
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• v.9 no.12
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• pp.286-293
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• 2019

In this study, four types of retinol, retinyl palmitate, adenocin, and polytoxylate-dretinamide, which are the ingredients of the Ministry of Food and Drug Safety Notice, included in the study. also we looked at trends in research on Sciadopitys verticillata, Prunella vulgaris, Celosia cristata L., Brazilin, Persicaria hydropiper, Astragalus membranaceus Bunge, Forsythiae Fructus, Lithospermum root, Rheum undulatum L. and Cistanche deserticola Y. C. Ma a natural material that has the efficacy of antioxidant aging. The anti-aging study so far has been found to be centered mainly on collagen production and elastase synthesis inhibition mechanisms. However, given that the aging process of the skin is caused by various ageing processes, it is believed that anti-aging studies using safe and effective natural materials that can help the skin age with various mechanisms should be conducted.

• Journal of Convergence for Information Technology
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• v.10 no.7
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• pp.249-256
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• 2020

To investigate the natural cosmetic ingredients of Benincasa hispida seed extract on skin care, we measured anti-oxidant and anti-inflammatory, and whitening effect. DPPH free radical scavenging activity was increased in a dose-dependent manner. The total phenolic content was higher in methanol extract (22.42±0.002 mgGAE/g) than water extract (9.77±0.002 mgGAE/g). MTT assay was demonstrated that the seed extract did not have a cytotoxic effect in RAW264.7 and B16F10 cell lines. We also examined to find out the inhibitory activity on NO production and secretion of TNF-α cytokine in LPS-induced RAW264.7 cells. In B16F10 melanoma cells, the seed extract significantly suppressed α-MSH induced melanin synthesis. Furthermore, westernblot analysis revealed that methanol extract dramatically downregulated the expression level of MITF, TRP-1 and TRP-2. Taken together, the B. hispida seed extract posses anti-oxidant, anti-inflammatory and skin whitening activities, which might provided its functional efficacy in cosmetic materials.

• The Korea Journal of Herbology
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• v.20 no.3
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• pp.19-27
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• 2005

Objectives : The antioxidant activities of Yeonkyo-san and its ingredient were investigated for industrial application. Methods : We experimented radical scavenging effect, superoxide dismutase and cytotoxicity effect of various cancer cell. Results : The result were obtained as follows : Primary testing of cytotoxicity of Yeonkyo-san and ingredient was done for G361. B16F10, MDA and A549 cell lines. Yeonkyo-san and ingredient water extract, ethanol extract showed cytotoxicity of over 90% respectively against cell line. The electron donating ability(EDA) of water extract from the Yeonkyo-san was higher than 60%, ethanol extract from the Yeonkyo-san was over 80% at 1000 ppm. The electron donating ability(EDA) of water extract and ethanol extract from the Yeonkyo-san ingredients were increased as well. SOD-like activity was high as 75% in 700 ppm, it was increased. All the other samples showed less than 40% SOD-like activity. Conclusions : The results indicated that water extract and ethanol extract of Yeonkyo-san and ingredient can be used as a natural ingredient with biological functions in cosmetic and food composition.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.4
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• pp.339-348
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• 2020

The purpose of this study was to investigate the possible use of the Boehmeria nivea var. nipononivea extract and fractions for the development of natural cosmetic ingredients. The leaves of B. nivea var. nipononivea, extracted by 70% ethanol, were sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and n-butanol. As a result of DPPH and ABTS test, ethyl acetate fractionation was shown to be excellent in radical scavenging activity. For the antimicrobial activities against Staphylococcus aureus, Staphylococcus epidermidis, Cutibacterium acnes and antibiotic resistant strains, MIC and birth control rate were observed by paper disc method. In the antibacterial activity by the disc diffusion assay against S. aureus, S. epidermidis and C. acnes, the dichloromethane and ethylacetate fraction showed stronger antibacterial activity than the other fractions and extract. Moreover, the ethylacetate fraction showed strong nitric oxide (NO) production inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell. In conclusion, we found that B. nivea var. nipononivea extract was not cytotoxic and showed antioxidant, antimicrobial and anti-inflammatory effects. These results suggest that the Boehmeria nivea var. nipononivea extract and fractions could be applied as an effective cosmetic material with antioxidant activity.

• Applied Chemistry for Engineering
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• v.29 no.3
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• pp.342-349
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• 2018

In this study, the physicochemical properties, emulsifying capacity, moisture content and cytotoxicity of the composite material produced by transesterification reactions of the olive oil (olive oil esters) were investigated for cosmetic applications. Olive oil esters with short (S) and long (L) reaction times were studied. From the TLC-image analysis, composition ratios of the olive oil esters S were found to be 5.2, 24.1, 46.4, and 21.9% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. Those of the olive oil esters L were 4.1, 24.7, 40.6, and 28.8% for mono-, di-, tri-glyceride, and fatty acid ethyl ester, respectively. The iodine value, acid value, saponification value, unsaponified matter, refractive index, and specific gravity were determined and purity tests were also carried out and normalized to establish standards and testing methods for using olive oil esters in cosmetics. To evaluate their emulsifying capacities, the O/W emulsion was prepared without surfactants and the formation of the emulsified particles were confirmed. After 5 days of applying the olive oil esters to human skin, the skin moisture retention was improved by 13.1% from the initial state. For the evaluation of toxicity on human skin cells, the olive oil esters showed 90% or more of the cell viability at $0.2-200{\mu}g/mL$. These results suggested that olive oil esters can be applied as natural/non-toxic ingredients to cosmetics industries.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.46 no.3
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• pp.207-218
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• 2020

In this study, the anti-inflammatory and anti-bacterial activities of the extracts from the leaves of the Hydrangea petiolaris were identified, and the chemical structure was identified by separating the active ingredient. As the result of the anti-inflammatory activity experiment using RAW 264.7 cells, it was confirmed that the n-hexane (Hex) and ethyl acetate (EtOAc) fractions inhibited the production of nitric oxide (NO) and the expression of iNOS protein in a concentration-dependent manner without cytotoxicity. In addition, the n-Hex and EtOAc fractions reduced the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6). Upon the anti-bacterial tests using Staphylococcus epidermidis and Cutibacterium acnes, the extract, n-Hex, EtOAc and n-butanol (BuOH) fractions showed potent activities. In order to isolate the active constituents, the n-Hex and EtOAc fractions were further purified to afford four phytochemicals; phytol (1), corosolic acid (2), asiatic acid (3) and 1-O-p-coumaroyl-β-D-glucopyranoside (4). All of the compounds 1 - 4 were isolated for the first time from this plant. In addition, the contents of isolated compounds were determined by HPLC and the quantity of phytol (1) was 27.8 mg/g for the 70% EtOH extract. Based on the above research results, it is believed that it will be possible to develop a natural cosmetic material that has anti-inflammatory and anti-bacterial effects using the extract of H. petiolaris leaves.