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http://dx.doi.org/10.22156/CS4SMB.2020.10.07.249

Anti-oxidant, anti-inflammatory and Whitening effect of Benincasa hispida seed extract  

Park, Gyu-Ri (Dept. of Beauty Art, Graduate School, Seokyeong University)
Lee, Ji-An (Dept. of Beauty Art, Graduate School, Seokyeong University)
Publication Information
Journal of Convergence for Information Technology / v.10, no.7, 2020 , pp. 249-256 More about this Journal
Abstract
To investigate the natural cosmetic ingredients of Benincasa hispida seed extract on skin care, we measured anti-oxidant and anti-inflammatory, and whitening effect. DPPH free radical scavenging activity was increased in a dose-dependent manner. The total phenolic content was higher in methanol extract (22.42±0.002 mgGAE/g) than water extract (9.77±0.002 mgGAE/g). MTT assay was demonstrated that the seed extract did not have a cytotoxic effect in RAW264.7 and B16F10 cell lines. We also examined to find out the inhibitory activity on NO production and secretion of TNF-α cytokine in LPS-induced RAW264.7 cells. In B16F10 melanoma cells, the seed extract significantly suppressed α-MSH induced melanin synthesis. Furthermore, westernblot analysis revealed that methanol extract dramatically downregulated the expression level of MITF, TRP-1 and TRP-2. Taken together, the B. hispida seed extract posses anti-oxidant, anti-inflammatory and skin whitening activities, which might provided its functional efficacy in cosmetic materials.
Keywords
Anti-oxidant; Anti-inflammatory; Whitening; Cosmeceutical; Benincasa hispida seed;
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Times Cited By KSCI : 6  (Citation Analysis)
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