• 센서학회지
• /
• 제13권5호
• /
• pp.342-349
• /
• 2004

SK (SPR Kinds) index, which can categorize the complicated measurement methods of surface plasmon resonance by simple method, has been proposed and verified. SK index is composed of three digits, where each digit presents the type of immobilized ligand, the type of illumination and the kinds of varying parameter, sequentially. The measurement method of (33#) series among SK indices shows the possibility of the multi-sensing capability, by which the response of 2-dimensional array of immobilized ligands can be detected simultaneously. The proposed possibility of multi-sensing capability has been verified by the modeling that is based on Fresnel reflection model.

• 센서학회지
• /
• 제15권5호
• /
• pp.347-351
• /
• 2006

A novel surface plasmon resonance sensor, which can measure 2-dimensional array of immobilized ligands without using imaging devices such as CCD, has been proposed. Regular surface plasmon resonance can be directly used due to the insertion of additional layers with different thickness, on which each ligands are immobilized. Surface plasmon resonance signals are separated depending on the thickness of additional layers. The possibility of multi-sensing capability of the proposed surface plasmon resonance sensor has been verified by the modeling that is based on Fresnel reflection model.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회A
• /
• pp.1617-1622
• /
• 2008

We report a new molecular detection process which measures the changes in the plasmon resonance peaks of periodic Au nanoparticle arrays fabricated using the electron beam lithography. As the Au nanoparticle arrays are modified by the chemical reaction in solutions having various concentrations of a target molecule, both the position and intensity of the plasmon peak change in proportion to the concentration of the target molecule. We expect that the process developed in this work can be employed for fine tuning of the plasmon peak wavelength and also for the optical detection of various kinds of molecules. Moreover, this method may improve the measurement accuracy compared with existing approaches that use only one change (peak wavelength or peak intensity) as a readout value for the molecular detection.

• The Plant Pathology Journal
• /
• 제34권2호
• /
• pp.85-92
• /
• 2018

Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QD-based biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.

• Journal of Microbiology and Biotechnology
• /
• 제20권12호
• /
• pp.1769-1771
• /
• 2010

The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.

• Applied Science and Convergence Technology
• /
• 제23권1호
• /
• pp.1-13
• /
• 2014

Semiconductor quantum structures are often characterized by their energy gaps which are modified by the quantum size effect. Energy levels in semiconductors can be realized by optical transitions within confined structures. Photoluminescence spectroscopy in magnetic fields at low temperatures has proved to be a powerful technique for investigating the electronic states of quantum semiconductor heterostructures and offers a complimentary tool to electrical transport studies. In this review, we examine comprehensive investigations of magneto-excitonic and Landau transitions in a large variety of undoped and doped quantum-well structures. Strong magnetic fields change the diamagnetic energy shift of free excitons from quadratic to linear in B in undoped single quantum well samples. Two-dimensional electron gas induced by modulation doping shows pronounce quantum oscillations in integer quantum Hall regime and discontinuous transition at ${\nu}=1$. Such discontinuous transition can be explained as the formation of spin waves or Skyrmions.

• Interaction and multiscale mechanics
• /
• 제3권2호
• /
• pp.111-121
• /
• 2010

Dynamic analysis of nano/micro bio-sensors based on a multiscale atomistic/continuum theory is introduced. We use a generalized atomistic finite element method (GAFEM) to analyze a bio-sensor which has $3{\times}N_a{\times}N_p$ degrees of freedom, where $N_p$ is the number of representative unit cells and $N_a$ is the number of atoms per unit cell. The stiffness matrix is derived from interatomic potential between pairs of atoms. This work contains two studies: (1) the resonance analysis of nano bio-sensors with different amount of target analyte and (2) the dependence of resonance frequency on finite element mesh. We also examine the Courant-Friedrichs-Lewy (CFL) condition based on the highest resonance frequency. The CFL condition is the criterion for the time step used in the dynamic analysis by GAFEM. Our studies can be utilized to predict the performance of micro/nano bio-sensors from atomistic perspective.

• Advances in nano research
• /
• 제14권6호
• /
• pp.527-539
• /
• 2023

Measuring energy burn during intensive combat sport has been a challenging concerns for a long time. In the present article, the energy consumption during combat sports is measured by use of wearable GoBe2 equipped with nanotechnology measuring devices. In this regard, 12 professional combat athletes were asked to wear GoBe2 devices during different sessions of intensive combat exercises. The curves provided by GoBe2 nano-sensor devices are further collected and analyzed for different combat durations. On the other hand, energy consumption in these athlete is calculated using other validated methods to evaluate reliability of GoBe2 wearable devices. Based on the results obtained from these experiments a multi-parameter mathematical model is presented for estimation of combat calorie consumptions. The results show that nanotechnology in these type of sensors could help in estimation of calorie consumption during combat. Moreover, the reliability of using wearable GoBe2 sensors are satisfactory except for some specific conditions. The mathematical model provides a satisfactory results based on athlete physical condition and also duration of the combat with about 8% error margin in the results.

• Journal of Electrochemical Science and Technology
• /
• 제15권1호
• /
• pp.161-167
• /
• 2024

Despite the important role of manganese (Mn) in cobalt-free, Ni-rich cathode materials, existing reports on the effects of Mn as a substitute for cobalt are not consistent. In this work, we analyzed the performance of cathodes comprised of Li(Ni_1-xMn_x)O₂ (LNMO). Both beneficial and detrimental results occurred as a result of the Mn substitution. We found that a complex interplay of effects (Li/Ni mixing driven by magnetic frustration, grain growth suppression, and retarded lithium insertion/extraction kinetics) influenced the performance and was intimately related to calcination temperature. This indicates the importance of establishing an optimal reaction temperature for the development of high-performance LNMO.

• 생명과학회지
• /
• 제18권2호
• /
• pp.234-240
• /
• 2008

매향' 딸기의 안토시아닌 생합성은 개화 후 26일째 시작되어 과실의 성숙기 동안 계속된다. 딸기로부터 안토시아닌의 생합성에 관여하는 주요 유전자를 분리하였다. 각각의 유전자에 대해, 다양한 식물체의 유사 유전자의 염기서열을 비교하여 PCR (polymerase chain reaciton) primer를 제작하였다. 숙기의 딸기에서 분리된 total RNA로부터 합성된 CDNA와 각 primer를 이용하여 RT (reverse transcriptase)-PCR을 수행하였다. 각 CDNA clone의 염기서열을 작성하여 분석한 결과, 이들은 안토시아닌 생합성에 관여하는 phenylalanine ammonia lyase (PAL), 4-cummarate CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone-3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanidine synthase (ANS) 그리고 UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT) 효소에 해당되었다. Northern blot 분석 결과, 이들 유전자는 과실 발달과정에서 시기적으로 조절되었다. 특히 PAL을 제외한 모든 유전자는 과실에서만 주로 발현되었다. PAL, DFR 그리고 ANS유전자는 과실 초기 발달 단계인 개화 후 10일에 검출된 후 감소하다가, 22일에 다시 증가하기 시작하여 34일에 최대가 되었다. 한편, 다른 유전자들은 초기에는 발현되지 않다가, 안토시아닌이 축적되기 시작하는 개화 후 $22{\sim}30$일에 처음으로 검출되었다. 본 연구를 통해, 딸기 과실 발달과정에서 안토시아닌 생합성 과정에 관여하는 여러 유전자가 과실 숙기에 함께 조절되는 현상을 알 수 있다. 이러한 연구 결과는 안토시아닌 합성과정을 제어하는 조절 유전자가 존재한다는 것을 시사한다. 그리고 딸기의 안토시아닌 생합성 유전자의 발현패턴을 크게 두 가지로 나눌 수 있는 것으로 보아, 딸기의 안토시아닌 생합성에는 적어도 두 가지 서로 다른 조절 기작이 관여하여 색소 발달 과정을 제어할 것으로 보인다.