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http://dx.doi.org/10.4014/jmb.1007.07036

Selection of Peptides Binding to HCV E2 and Inhibiting Viral Infectivity  

Hong, Hye-Won (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Lee, Seong-Wook (Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University)
Myung, Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.12, 2010 , pp. 1769-1771 More about this Journal
Abstract
The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.
Keywords
Hepatitis C virus; E2; CD81; infection; peptide;
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