Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Selection of Peptides Binding to HCV E2 and Inhibiting Viral Infectivity

  • Hong, Hye-Won (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Lee, Seong-Wook (Department of Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University) ;
  • Myung, Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • Received : 2010.07.19
  • Accepted : 2010.09.06
  • Published : 2010.12.28
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Abstract

The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.

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